• Title/Summary/Keyword: microscope image processing

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Simple optical microscope using Light Field Rendering technology (Light Field Rendering 기술을 이용한 간이 광학현미경)

  • Kim, Da-Hee;Cho, Joong-Hwee
    • Proceedings of the Korea Information Processing Society Conference
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    • 2020.11a
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    • pp.725-728
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    • 2020
  • Light Field Rendering 을 이용하여 성능이 떨어지는 Low resolution 카메라 장비로 얻는 영상의 한계를 극복하고, Image Processing 기술로 직접 조정해야 해결할 수 있는 수차 및 성능 문제 해결한다. 저가형 장비, 렌즈를 사용하여도 컴퓨터기반 처리를 이용하여 물리적인 한계를 극복한 간이 광학현미경을 만들고자 함. 3D print 를 이용한 뼈대구조를 만들고, 저렴한 raspberry pi 임베디드 플랫폼을 이용하여 설계도만 있다면, 누구나 쉽게 만들 수 있기에 많은 사람들이 이 분야에 더 쉽게 다가설 수 있게 한다.

Evaluation of Crack Monitoring Field Application of Self-healing Concrete Water Tank Using Image Processing Techniques (이미지 처리 기법을 이용한 자기치유 콘크리트 수조의 균열 모니터링 현장적용 평가)

  • Sang-Hyuk, Oh;Dae-Joong, Moon
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.10 no.4
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    • pp.593-599
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    • 2022
  • In this study, a crack monitoring system capable of detecting cracks based on image processing techniques was developed to effectively check cracks, which are the main damage of concrete structures, and a program capable of imaging and analyzing cracks was developed using machine vision. This system provides objective and quantitative data by replacing the appearance inspection that checks cracks with the naked eye. The verification of the development system was applied to the construction site of a self-healing concrete water tank to monitor the crack and the amount of change in the crack width according to age. In the case of crack width detected by image analysis, the difference from the measured value using a digital microscope was up to 0.036 mm, and the crack healing effect of self-healing concrete could be confirmed through the reduction of crack width.

Development of Line Standards Measurement System Using an Optical Microscope (광학 현미경을 이용한 선표준물 측정 시스템 개발)

  • Kim, Jong-Ahn;Kim, Jae-Wan;Kang, Chu-Shik;Eom, Tae-Bong
    • Journal of the Korean Society for Precision Engineering
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    • v.26 no.8
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    • pp.72-78
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    • 2009
  • We developed a line standards measurement system using an optical microscope and measured two kinds of line standards. It consists of three main parts: an optical microscope module including a CCD camera, a stage system with a linear encoder, and a measurement program for a microscopic image processing. The magnification of microscope part was calibrated using one-dimensional gratings and the angular motion of stage was measured to estimate the Abbe error. The threshold level in line width measurement was determined by comparing with certified values of a line width reference specimen, and its validity was proved through the measurement of another line width specimen. The expanded uncertainty (k=2) was about 100 nm in the measurements of $1{\mu}m{\sim}10{\mu}m$ line width. In the comparison results of line spacing measurement, two kinds of values were coincide within the expanded uncertainty, which were obtained by the one-dimensional measuring machine in KRISS and the line standards measurement system. The expanded uncertainty (k=2) in the line spacing measurement was estimated as $\sqrt{(0.098{\mu}m)^2+(1.8{\times}10^{-4}{\times}L)^2}$. Therefore, it will be applied effectively to the calibration of line standards, such as line width and line spacing, with the expanded uncertainty of several hundreds nanometer.

The Enhancement of Inner-solid Image by the Bandwidth Increment of Vertically Spatial Frequency (축 방향 공간주파수 대역의 확장을 통한 고체 내부영상 개선)

  • Koo, Kil-Mo;Kim, Sang-Baik;Kim, Hyun;Jun, Kye-Suk
    • Proceedings of the KSME Conference
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    • 2001.06b
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    • pp.176-180
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    • 2001
  • In this paper, we have studies the images have been reconstructed by using combination of images which has been acquired by the variation of operating frequency. When inner images has been reconstructed, inner image has been superposition by surface state effect. In this case, image enhancement the phase object and enhance the contrast of inner image. In the result of the specimen for the round defect with 2mm diameter, for the types of the depth are 1.5mm, 2mm, 2.5mm, and 3mm, it has been show that the shape of defect has better than before this processing and phase contrast grow large twice. And we have constructed the acoustic microscope by using quadrature detector that is able simultaneously to acquired the amplitude and phase of the reflected signal. Father more we have studied the reconstruction method of the amplitude and phase images and the enhancement method of the defect images' contrast.

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Confocal Microscopy Measurement of the Fiber Orientation in Short Fiber Reinforced Plastics

  • Lee, Kwang Seok;Lee, Seok Won;Youn, Jae Ryoun;Kang, Tae Jin;Chung, Kwansoo
    • Fibers and Polymers
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    • v.2 no.1
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    • pp.163-172
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    • 2001
  • To determine three-dimensional fiber orientation states in injection-molded short fiber composites a CLSM (Confocal Laser Scanning Microscope) is used. Since the CLSM optically sections the composites, more than two cross-sections either on or below the surface of the composite can be obtained. Three dimensional fiber orientation states can be determined with geometric parameters of fibers on two parallel cross-sections. For experiment, carbon fiber reinforced polystyrene is examined by the CLSM. Geometric parameters of fibers are measured by image analysis. In order to compactly describe fiber orientation states, orientation tensors are used. Orientation tensors are determined at different positions of the prepared specimen. Three dimensional orientation states are obtained without the difficulty in determining the out-of-plane angles by utilizing images on two parallel planes acquired by the CLSM. Orientation states are different at different positions and show the shell-core structure along the thickness of the specimen.

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Measurement Algorithms of Sizing removed state using Image Process And Development of Carbon fibers with Electromagnetic shielding Performance (영상처리를 이용한 사이징 제거 상태 측정 알고리즘과 전자파 차폐 성능을 갖는 탄소 섬유 개발)

  • Cho, Joon-Ho;Jeon, Kwan-Goo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.2
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    • pp.95-101
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    • 2017
  • In this paper, the sizing removal condition for the pretreatment of composite materials is obtained numerically by applying an image processing algorithm and nickel-plated carbon fiber is fabricated by a dry process method to enhance its electromagnetic shielding performance. Sizings that are wrapped in a polymer type material during the manufacturing of carbon fiber should be removed for dry coating. A numerical value, that is the correlation, can be obtained by determining the regular pattern of the carbon fiber in the image taken by a scanning electron microscope (SEM) after the sizing is removed. The application of the proposed numerical method to the SEM image of the fiber after the sizing is removed with solution, compressed air, solution and compressed air (hybrid), showed that this method of eliminating the sizing is superior to the hybrid method. Then, by spreading the carbon fiber roll with the sizing removed, we were able to produce nickel plated carbon fiber by the roll-to-roll sputtering method. The electromagnetic shielding performance of the fabricated 30, 40 and 100 nickel coated carbon fibers was measured. The Korea Advanced Institute of Science and Technology evaluated the electromagnetic shielding performance of the 100 nickel-coated carbon fiber to have a maximum value of 73.2 (dB) and a minimum value of 66.7 (dB). This is similar to the electromagnetic shielding rate of copper and shows that this material can be used as a cable for EV / HEV automobiles.

Design and Implementation of the System for Automatic Classification of Blood Cell By Image Analysis (영상분석을 통한 혈구자동분류 시스템의 설계 및 구현)

  • Kim, Kyung-Su;Kim, Pan-Koo
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.36C no.12
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    • pp.90-97
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    • 1999
  • Recently, there have been many researches to automate processing and analysing image data in medical field, due to the advance of image processing techniques, the fast communication network and high performance hardware. In this paper, we design and implement the system based on the multi-layer neural network model to be able to analyze, differentiate and count blood cells in the peripheral blood image. To do these, we segment red and white-blood cell in blood image acquired from microscope with CCD(Charge-coupled device) camera and then apply the various feature extraction algorithms to classify. In addition to, we reduce multi-variate feature number using PCA(Principle Component Analysis) to construct more efficient classifier. So, in this paper, we are sure that the proposed system can be applied to a pathological guided system.

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A Study on Droplet Distribution of Bio Diesel Fuels Using Immersion Sampling Method (액침법에 의한 바이오디젤유의 액적분포에 관한 연구)

  • Kim, M.S.;Doh, H.C.;Koh, D.K.;Ahn, S.K.
    • Journal of Power System Engineering
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    • v.10 no.4
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    • pp.5-10
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    • 2006
  • The purpose of this study is to measure the droplet distribution and Sauter mean diameter(SMD) of biodiesel fuel, using the immersion sampling method. This method involves using an optical microscope and a CCD camera, to take an image of the droplets. These images are then measured by using a 'Sigma Scan' processing program. The results of the above experiment are summarized as followed ; (1) There can be as much as a 10% error rate when measuring the diameter of these droplets, using the image processing method and the naked eye. (2) The result of droplet size distribution test, TVO(transesterified vegetable oil) big size droplet distribution were increased at ambient pressure $6kg/cm^2$. (3) When ambient pressure increased $6kg/cm^2$ above, SMD variation of TVO and UVO(used vegetable oil) 30 are small. (4) On Rosin-Rammler analysis, droplets size distribution of UVO(used vegetable oil) 30 uniform more than TVO 20 on ambient pressure $1kg/cm^2$.

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Cell Counting Algorithm Using Radius Variation, Watershed and Distance Transform

  • Kim, Taehoon;Kim, Donggeun;Lee, Sangjoon
    • Journal of Information Processing Systems
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    • v.16 no.1
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    • pp.113-119
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    • 2020
  • This study proposed the structure of the cluster's cell counting algorithm for cell analysis. The image required for cell count is taken under a microscope. At present, the cell counting algorithm is reported to have a problem of low accuracy of results due to uneven shape and size clusters. To solve these problems, the proposed algorithm has a feature of calculating the number of cells in a cluster by applying a radius change analysis to the existing distance conversion and watershed algorithm. Later, cell counting algorithms are expected to yield reliable results if applied to the required field.

Vessel extraction in optical microscope image with non-uniform illumination (명도 불균형 현상을 가진 광학 현미경 영상에서의 혈관 추출)

  • Lee, Ji-Hye;Kim, Myoung-Hee
    • Proceedings of the Korea Information Processing Society Conference
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    • 2009.11a
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    • pp.371-372
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    • 2009
  • 세포단위에서 혈관 생성과정 관찰 시 사용되는 광학현미경 영상은 광원의 특성으로 인해 부분적으로 영상이 밝거나 혹은 어둡게 나타난다. 이러한 현상은 혈관 영상에서의 혈관 분석을 어렵게 하므로 본 논문에서는 신 혈관 생성 영상에서 명도 불균형 문제를 해결하는 혈관 추출 및 정량화 기법을 제안한다. 불균형 명도 문제를 해결하기 위해 지역적 문턱치화 방법을 사용하고, 잡음 및 혈관 내 틈새 문제를 해결하기 위해 형태학적 연산 처리를 하였다. 그 결과 명도가 어두운 지역에서 배경 잡음이 없고, 영역이 끊어지지 않고 하나로 연결된 혈관을 추출할 수 있었다. 추출된 혈관 영역에서 골격화와 혈관 세그먼트에 기반하여 두께, 길이, 넓이를 정량화함으로써 혈관 분석 시 현미경에 탑재되어 있어 가장 널리 사용되고 있는 메타 모르프보다 정확한 정량화 결과를 얻었다.