• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.29-35
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• 2009

The study was conducted to examine effective concentration and fertilization time of $CO_2$ for the growth of potted Phalaenopsis in growth chambers. Enrichment level of $CO_2$ was 1,000 or $2,000{\mu}mol{\cdot}mol^{-1}$ and fertilization time was 06:00~12:00, 00:00~06:00, or 18:00~24:00. Two, seven, or twelve month-old clonal micropropagules of Phalaenopsis cultivars were cultured for 99 days. Leaf number, leaf length, leaf width, root length and fresh weight of all Phalaenopsis cultivars in $2,000{\mu}mol{\cdot}mol^{-1}$ $CO_2$ were significantly greater than that of $1,000{\mu}mol{\cdot}mol^{-1}\;CO_2$. $CO_2$ fertilization time was the greatest growth in 0:00~06:00.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.127-130
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• 1995

Japanese pepper (Zanthoxylum piperitum Dc.) tree of selected genotype was propagated by a two-step method. Among the media tested, BTM promoted shoot height growth and DKW revealed as superior to micro-canopy increment Multiple shoot formation was greatest when the single shoot were subcultured on medium containing 0.89 $\mu$M BA alone. The numbers of survival shoots could be markedly increased by acclimatization of the multiplied shoots itself in plastic Petridish (punctured with pin on the top) for two weeks and subsequently transplanting each shoot onto peat plug system. After transplanting the micropropagules onto pots, prickliness characteristics seem to be transmitted to all plane produced from selected genotype.

• Journal of People, Plants, and Environment
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• v.23 no.5
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• pp.545-554
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• 2020

Background and objective: Moth orchids in the vegetative stage are suitable for a multi-layer growing environment in a closed-type plant factory which can be a good alternative that can reduce production costs by reducing cultivation time and energy cost per plant. This study was conducted to find out the optimal rhizospheric environment for different irrigation methods without a potting medium and rhizospheric ventilation for the vegetative growth of young Phalaenopsis hybrid 'Blanc Rouge' (P. KV600 × P. Kang 1) and Phalaenopsis Queen Beer 'Mantefon' in a closed-type plant factory system. Methods: The one-month-old clonal micropropagules with bare roots rapped with a sponges were fixed on the holes of styrofoam plates above growth beds, and were watered using the ebb-and-flow (EBB) and aeroponic (AER) methods with Ichihashi solution (0.5 strength) once a day at 06:00 (P) or 18:00 (S), and both (PS). Rhizospheric ventilation (V) was also applied to change the temperature, relative humidity, and CO₂ concentration of the beds. Plants potted into sphagnum moss and watered once a week were used as the control group. Results: After 12 months of treatment, the growth characteristics of the EBB groups were the best among the treatment groups without a medium, but no effect of irrigation timing was observed. V reduced the temperature, relative humidity and CO₂ concentration of the beds. Whereas, EBB+V (ebb-and-flow with ventilation) improved plant growth and reduced the occurrence of disorders and withering. Especially, EBB+V showed a similar performance to the control group. Conclusion: The results indicated that the optimal irrigation method without a potting medium for producing middle-aged potted moth orchids was the EBB system with forced rhizospheric ventilation. Therefore, further studies on the optimal ventilation method and moisture control of the crown need to be carried out to develop the irrigation system without a potting medium for vertical farming in closed-type plant factories.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.191-204
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• 1994

Due to its topographic complexities and various climatical condition, Korea exhibits diverse forest types. Dominant tree species in this zone are Quercus spp., Betula spp., Zelkova spp., Fraxinus spp., Pinus densiflora, Pinus koraiensis, and Pinus thunbergii ete. Genetic conservation in forest species in Korea there are three ways ; one is in situ, other is ex situ and third is in-facility conservation. In situ conservation include that are the present status of conservation of rare and endangered flora and ecosystem, the reserved forest, the national and provincial park, and the gene pool of natural forests. Ex situ conservation means to be established the new forest from in situ forest stands, progeny and provenance test populations, seed orchard and clone banks, and gene conservation in-facility. As a tool for low temperature storage, several aspects on in vitro system were studied ; (1) establishment of in vitro cultures from juvenile and/or rejuvenated tissues, (2) induction of multiple shoots from the individual micropropagules, (3) elongation of the proliferated shoots. Studies on cold storage for short-and long-term maintenance of in vitro cultures under $4^{\circ}C$ in the refrigerator were conducted. For the cryopreservation at $-196^{\circ}C$, various factors affecting survivability of the plant materials are being examined. The necessity of gene conservation of forest trees is enlarged not only to increase the adaptability for various environments but also to gain the breeding materials in the future. For effective gene conservation of forest trees, I would like to suggest followings ; 1. Forest stands reserved for other than the gene conservation purposes such as national parks should be investigated by botanical and gene-ecological studies for selecting bio-diversity and gene conservation stands. 2. Reserved forest for gene pool should be extented both economically important tree spp. and non-economical species. 3. Reserved forest for progeny test and clone bank should be systematically investigated for the use of Ex situ forest gene conservation. 4. We have to find out a new methodology of genetic analysis determining the proper and effective size of subpopulation for in situ gene conservation. 5. We should develop a new tree breeding systems for successful gene conservation and utilization of the genetic resources. 6. New method of in-facility gene conservation using advanced genetic engineering should be developed to save time and economic resources. 7. For the conservation of species with short-life span of seed or shortage of knowledge of seed physiology, tissue culture techniques will be played a great role for gene conservation of those species. 8. It is are very useful conservation not only of genes but of genotypes which were selected already by breeding program. 9. Institutional and administrative arrangements including legistlation must be necessarily taken for gene conservation of forest trees. 10. It is national problems for conservation of forest resources which have been rapidly destroyed because of degenerating environmental condition and of inexperienced management system of bio-diversity and gene conservation. 11. In order to international cooperation for exchanging data of bio-diversity and gene conservation, we should connect to international net works as soon as possible.