• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.1045-1054
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• 1996

Tissue culture, or micropropagation , is being used for the vegetative multiplication of several hundred millions of superior plants annually for horticulture and forestry. It is often more expensive than other forms of propagation using cuttings or seeds, because it is labor intensive and more specialized . The aim of automation is to reduce the cost per plantlet by reducing labor input, and finally, to yield profit, as business activity . Labor usually account for 70-80% of th ein vitro and ex vitro cost. This paper aspects of tissue culture automization , such as technical and economical approaches in view of automization.

• Journal of Plant Biotechnology
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• v.6 no.3
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• pp.165-169
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• 2004

The fruits of Vitex rotundifolia in Korea, known as 'Man Hyung Ja', occupy an important position as traditional oriental medicine in Asian countries. It is known that propagation of this plant by seed is difficult and time-consuming with little success. Attempts were made to develop a method by using nodal culture techniques. Explants of stem node without leaves cultured on Nitsch medium containing 1 ml/L BA, gave the best shoot induction ratio. Also, BA with IAA or TDZ treatment showed positive effect on shoot induction. Half-strength Nitsch medium was supplemented with 0.5 mg/L NAA produced better success than did the others on root formation. It showed that many of the regenerants grew successfully on growth chamber at $24^{\circ}c$.

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.252-253
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• 2010

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.17-22
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• 1994

This study was carried out to investigate the optimum medium and concentrations of growth regulators for induction of multiple shoot by meristem culture of floe otorefcenf Mill. MS medium supple-mented with 3${\mu}{\textrm}{m}$ TDZ was effective for induction of multiple shoot. Shoot multiplication was more ef-fective when 2mg/1 BA combined with 0.Img/1 IAA than when only BA were treated on medium. Halfstrength of MS medium supplemented with 2mg/L IAA was effective for rooting of shoots regenerated.When plantlets regenerated from meristem culture were transferred to pot, survival rate of plantletswas 80% on perlite and was 95% on vermiculite, respectively.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.115-119
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• 2003

To determine the appropriate concentrations of nutrients and growth regulators for shoot proliferation and root initiation, several rose hybrid tea cultivars were cultured. Cultured shoot tips and lateral buds from different cultivars proliferated multiple shoots on Murashige and Skoog (MS) medium supplemented with 0 to 4 mg/L BA and 0 to 0.05 mg/L NAA. The ability of the explants to proliferate shoots and initiate roots was affected by genotype, the nodal position of explant, the strength of MS basal medium and growth regulators used. The buds nearest the apex exhibited the slowest rate of development. Most cultivars had the highest shoot proliferation when cultured on MS medium with 2 mg/L BA and 0.01 mg/L NAA, but the degree varied by cultivars. Root development was enhanced by lowering the concentration of MS salts.

• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.173-178
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• 2021

Basal callus formation and leaf abscission is a problem in clonal micropropagation. We have described an in vitro clonal propagation protocol of Dalbergia sissoo Roxb (shisham) 'FRI-14' in which AgNO₃ played important role not only in mitigating problem of leaf abscission and basal callus, but also improved shoot induction and multiplication. Best induction and shoot multiplication was obtained on MS media with 1.5 mg/l 6-BAP and 10 mg/l AgNO₃ and half-strength MS media with 0.5 mg/l 6-BAP, 2 mg/l AgNO₃ and 50 mg/l Adenine sulphate whereas best ex vitro rooting was obtained with 200 mg/l IBA in pulse treatment.

• Journal of Forest and Environmental Science
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• v.26 no.1
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• pp.31-36
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• 2010

Sixteen microsatellite markers (simple sequence repeat (SSR) markers) were employed to examine the genetic stability of 27 randomly chosen date palm (Phoenix dactylifera L.) plants produced through somatic embryogenesis with upto forty two in vitro subcultures. No microsatellite DNA variation was observed among all micropropagated plants. Our results indicate that the micropropagation protocol used for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated that somatic embryogenesis can also be used as one of the safe modes for production of true-to-type plants of date palm. This is the first report on the use of microsatellite DNA markers to establish the genetic stability in micropropagated date palm plants.

• Journal of Plant Biotechnology
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• v.50
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• pp.255-266
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• 2023

Hyperhydricity is a physiological anomaly that significantly affects the growth and proliferation rate of crops cultivated by tissue culture techniques. To better understand the mechanisms that govern hyperhydricity incidence, we examined the effects of several media components, particularly cytokinin and gelling agents. These elements were found to be influential in both in vitro propagation and the development of hyperhydricity. Our study revealed that Arabidopsis thaliana seedlings had a greater manifestation of hyperhydricity symptoms when exposed to high cytokinin concentrations compared with the control. The presence of gelrite led to the manifestation of hyperhydric symptoms by elevated water build-up in the apoplast. The phenomenon of stomata closure was observed in the hyperhydric leaves, resulting in an increased ability to retain water and a decrease in the transpiration rates when compared to their respective control leaves. Additionally, histological examinations of the cross sections of hyperhydric leaves revealed an irregular cellular arrangement and large intercellular spaces. Furthermore, hyperhydric seedlings displayed impaired cuticular development in comparison to their normal seedlings.

• Journal of Korean Society of Forest Science
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• v.82 no.1
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• pp.26-33
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• 1993

In vitro shoot proliferation and rooting were tested for 2-0 seedlings of half-sib families of 4 plus oaks trees. Nodal segments having axillary buds from 37 families(16 of Quercus acutissima, 10 of Q. variabilis, 7 of Q. serrata, and 4 of Q. mongolica) were cultured on WPM(Woody Plant Medium) supplemented with 0.5 mg/l BA (6-benzyladenine) and 0.01 mg/l NAA(${\alpha}$-naphthalene acetic acid) and subcultured at 2-3 weeks of intervals fur 6 months. In vitro rooting was carried out on GD(Gresshoff and Doy) medium supplemented with 0.5mg/l IBA(indole butyric acid). The capacity for shoot proliferation and rooting was highly varied with families. Generally, white oaks(Q. serrata and Q. mongolica) showed poor response than black oaks(Q. acutissima and Q, variabilis) in shoot proliferation and rooting. Among the total of 37 families, 7 of Q. acutissima, each 2 of Q. variabilis, Q. serrata, and Q. mongolica revealed abilities for continuous shoot proliferation, and the others failed to proliferate. Rooting of the selected oak trees also greatly varied among the families. In Q. acutissima, rooting ratio ranged from 10.0%(CB 25. KG 4) to 89.8%(CB 18). Although 26.7% of KG 16 in Q. variabilis, 3.3% of JN 15 in Q. serrata were rooted, Q. mongolica was not rooted at all in this experimental conditions. No relationship between shoot growth and the rooting ability was observed. Present results suggest the possibility of large-scale micropropagation, but further studies on family differences, shoot-tip necrosis, and callusing of rooting junction are still required to develop reliable micropropagation systems.

• Journal of Bio-Environment Control
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• v.18 no.1
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• pp.15-22
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• 2009

Adequate environment conditions with growth stage of potato were decided in a photoautotrophic micropropagation system using models. Total 20 day-period of growth were divided into three growth periods such as 6 (stage 1), 7(stage 2), and 7(stage 3) days. At the 1st stage, no significant differences were observed in the growth of potato plantlets at various photosynthetic photon flux density (PPFD) and $CO_2$ conditions. Considering damaged leaves, $80\;mmol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and ambient $CO_2$ level were adequate in this stage. At the 2nd stage, significant differences were partly observed in several growth characteristics including dry weight. Based on the dry matter model, over $240\;mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD was too high to cultivate potato plantlets at this stage due to the occurrence of damaged leaves. Considering both plant growth and energy efficiency, $160\;mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and $700\;mol{\cdot}mol^{-1}\;CO_2$ were selected for the adequate combination. At the 3rd stage, the biomass accumulation was significantly induced in potato plantlets under higher levels of PPFD and $CO_2$ concentration as suggested by increased fresh and dry weights. However, we could not find the saturated point with regard to dry matter due to continuous increase of dry mater even under maximum PPFD ($320\;mmol{\cdot}m^{-2}{\cdot}s^{-1})$. Thus, $320\;mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and $1800\;mol{\cdot}mol^{-1}\;CO_2$ were considered as the best choice at final stage in this study. In conclusion, even though the growth period of micropropagated potato plantlets was quite a short, favorable environmental conditions required at each growth stage were different. This technique could improve the growth of micropropagated plantlets compared to the conventional micropropagation and apply to other agriculturally important crops as well as potato in the future.