• Journal of Environmental Science International
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• v.7 no.3
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• pp.393-400
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• 1998

A microorganism, Klebsiella gr. 47, capable of degrading BTX(benzene, toluene and xylene) was isolated from oil-contaminated soil and its characteristics of BTX degradation were investigated. When benzene and toluene were fed to Klebstella gr. 47 simulataneously, they showed competitive ingibition. The degradation rate of xylene was enhanced as much as 3 times when xylene was fed with benzene or toluene. Degradation rate of benzene and toluene was also enhanced by cocultured with Alcaligenes xylosoxidans. When benzene-adapted microorganism was used, each BTX compound was degraded efficiently within 5 hours.

• Journal of the Korean Society of Tobacco Science
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• v.12 no.2
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• pp.85-90
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• 1990

Pot experiment was conducted to find out the effect of decomposing degree of rice straw on growth characteristics of flue-cured tobacco, NC82. Tobacco growth was hindered by fresh straw of rice application. Generally, it was known that if organic matter of high Carbon/Nitrogen ratio had applied in soil, there was temporary nitrogen deficiency in plant caused by soil microorganism utilized nitrogen contained organic matter. In pot experiment, it was supposed that tobacco growth hindered by fresh straw of rice application was not nitrogen deficiency by soil microorganism, but gas toxicity by fresh straw of rice application.

• Applied Chemistry for Engineering
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• v.20 no.3
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• pp.301-306
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• 2009

This research was carried out to establish the effective treatment condition and characteristic of wastewater from the electroplating plant of cold rolling mill by using microorganism. Alkaline wastewater and acidic heavy metal wastewater accounted for 64%, 30%, respectively, of the total wastewater. Highly concentrated thiocyanate was 53890 mg/L as COD and it was 53% of total COD, even though it was 0.03% of wastewater from the electroplating plant. When treating mixed wastewater with microorganism, it was easy to remove when SCN concentrations of mixed wastewater was 200 mg/L or less. While the treatment effect of COD-causing materials was low at the concentration of 400 mg/L or less, it implies that highly concentrated thiocyanate contains a large amount of slowly biodegradable organics. When treating with mixed wastewater, pH was 7.33 at the beginning, but after 8 hours it increased to 7.99. This is caused by ammonia which is generated when SCN of highly concentrated thiocyanate was degraded by microorganism.

• Journal of Korean Academy of Nursing
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• v.45 no.1
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• pp.46-53
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• 2015

Purpose: This study was conducted to examine the effect of oral care with an aroma solution on oral status and oral cavity microorganism growth in elderly patients with stroke. Methods: A non-equivalent control group, with a pretest-posttest design was used in this study. The participants were assigned to the experimental group (n=30) that received oral care with an aroma solution or the control group (n=31) that received 0.9% saline solution. To identify the effect of the experimental treatments, objective/subjective assessments of oral status and oral cavity microorganism growth were performed using the oral assessment guide, oral perception guide, and oral swab culture. Data were analyzed using Chi-square test, Fisher's exact test, and t-test with the SPSS version 21.0 program. Results: The objective oral status was significantly lower in the experimental group than in the control group (t= -3.64, p<.001). There was no significant difference between the subjective oral status of the experimental group and control groups (t= -1.24, p=.109). Oral microorganism growth was significantly lower in the experimental group than in the control group (t= -7.39, p<.001). Conclusion: These findings indicate that special mouth care using an aroma solution could be an effective oral health nursing intervention for elderly patients with stroke.

• The Korean Journal of Food And Nutrition
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• v.30 no.2
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• pp.203-210
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• 2017

The purpose of this study was to compare hand washing awareness and microorganism contamination on the hands for evaluating the difference between awareness and conditions of hand hygiene. The average number of total aerobic bacteria and coliform was 2.7 log CFU/hand and 1.8 log CFU/hand, respectively. Coliform bacteria were detected in all of the students tested in this study. The questionnaire survey data obtained via a direct interview were collected from 100 university students with microorganism analysis. A majority (90%) of the students recognized that good hand hygiene is important in order to prevent food poisoning. In the hand washing survey data, the responses suggesting "always washed" from "before eating food", "after handling money", "after finishing study" and "after visiting patients" were observed in 18, 13, 15 and 33 out of the 100 students, respectively. The questionnaire data of hand washing frequency and time duration showed that 14 students and 6 students answered "more than 8 times of hand washing per day" and "more than 30 seconds of hand washing time", respectively. These results indicated that improper hand washing practices and poor hand hygiene were identified in the majority of the students. This emphasizes the difference between awareness and conditions of hand hygiene. Repeated hand washing education about the frequency, time duration and methods is constantly required to improve hand hygiene. Installation of hand washing equipment in the space of a restaurant and cafeteria is suggested for enhancing hand hygiene before eating food.

• Korean Journal of Organic Agriculture
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• v.26 no.3
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• pp.427-437
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• 2018

In this study, microorganism community characteristics of organic managed soil which applied rye (Secale cereal L.) as green manure for 25 years, were determined. The chemical properties of organic soil showed high level of organic matter and available $P_2O_5$, while the level of exchangeable cation was low. The analysis of dehydrogenase activity and carbon source utilization indicated that the values in on organic soil were significantly higher than those of the control. It suggested that the microorganism community of organic soil had high microorganism activity, compared to the control. In addition, when the 16S rRNA gene-targeted NGS (Next generation sequencing) analysis was conducted to estimate the class of bacterial community, the class level of bacterial taxon composition on organic soil showed higher portion of Sphingobacteriia, Acidobacteriia, Gammaproteobacteria, Solibacteres and Planctomycetia. By base on the results of various reports in which organic managed soil had high portion of Acidobacteriia and Planctomycetia, the characteristic of taxon composition in organic soil, which showed the high percentages of Ktedonobacteria, Sphingobacteriia, Acidobacteriia and Gammaproteobacteria, was resulted from the application of rye as a green manure for the long term. However, further researches were needed because the crop effect was not considered in this study.

• Journal of Environmental Health Sciences
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• v.26 no.3
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• pp.1-10
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• 2000

Although microorganisms play an important role in composting process, researches on the effective microorganism (EM) in composting process are rarely reported. In this study, three stages of work performed 1) investigation of composting facility 2) sample collection and isolation of the EM 3) lab-scale composting by seeding the isolated EM. For this, purpose, physical, chemical, and biological characteristics, such as temperature, moisture content, organic matter, pH population of microorganism, etc., were investigated during the process and the results were compared with those of a control(non-seeded). In the composting facility, the most active degradation of food waste was suppsed to occur in 10-11th day of composting by observing the temperature and CO2 emission. The population of bacterial and fungi was highest in thermophilic stage. Meanwhile that of actinomycetes and yeast was relatively uniform during the process. In the lab-scale test, the thermophilic stage was maintained longer(more than 9 days in 17 days of experiment) in the seeded which was favored for the high reduction of organic matter and moisture. Reduction rates of lipids and salinity were also increased in the seeded. It confirmed these results that the population of microorganisms in the seeded was observed higher in several orders than the control. However, pH of the seeded was maintained as low as about 4.5 throughout the experiment except the final period of the process. Meanwhile, pH of the control rose in the early stage of the experiment. This was probably due that the seeded microorganisms collected from the composting facility was adapted to the low pH environment.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.77-83
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• 2006

A gene encoding a $\gamma-butyrolactone$ autoregulator receptor was cloned from Saccharopolyspora erythraea, and the biochemical characteristics, including the autoregulator specificity, were determined with the purified recombinant protein. Using primers designed for the conserved amino acid sequence of Streptomyces $\gamma-butyrolactone$ autoregulator receptors, a 120 bp S. erythraea DNA fragment was obtained by PCR. Southern and colony hybridization with the 120 bp fragment as a probe allowed to select a genomic clone of S. erythraea, pESG, harboring a 3.2 kb SacI fragment. Nucleotide sequencing analysis revealed a 615 bp open reading frame (ORF), showing moderate homology (identity, $31-34\%$; similarity, $45-47\%$) with the $\gamma-butyrolactone$ autoregulator receptors from Streptomyces sp., and this ORF was named seaR (Saccharopolyspora erythraea autoregulator receptor). The seaR/pET-3d plasmid was constructed to overexpress the recombinant SeaR protein (rSeaR) in Escherichia coli, and the rSeaR protein was purified to homogeneity by DEAE-Sephacel column chromatography, followed by DEAE-ion-exchange HPLC. The molecular mass of the purified rSeaR protein was 52 kDa by HPLC gel-filtration chromatography and 27 kDa by SDS-polyacrylamide gel electrophoresis, indicating that the rSeaR protein is present as a dimer. A binding assay with tritium-labeled autoregulators revealed that rSeaR has clear binding activity with a VB-C-type autoregulator as the most effective ligand, demonstrating for the first time that the erythromycin producer S. erythraea possesses a gene for the $\gamma-butyrolactone$autoregulator receptor.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1140-1145
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• 2005

MJM383, a rare actinomycete sp. strain originated from Chinese soils, was isolated through an antimicrobial screening system. The analysis of 16S rDNA sequences and biochemical characterization determined the strain to belong to genus Kitasatospora. Both NMR and ESI mass data of its purified bioactive compounds revealed Kitasatospora sp. MJM383 to produce two antitumor agents, streptonigrin and oxopropaline G, which have been known to be produced from Streptomyces species. This is the first report to demonstrate the presence of antitumor agents produced by genus Kitasatospora.

• The Korean Journal of Pesticide Science
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• v.13 no.1
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• pp.21-27
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• 2009

A study was carried out to find out the methodology of biological treatment for wastes and wastewater caused by an organophosporus insecticide, fenitrothion, using fenitrothion-degrading microorganism. A fenitrothion-degrading microorganism was isolated by using a selective nutrient broth (NB) medium including fenitrothion, and identified to Bacillus sphaericus NFol based on its morphological and biochemical characteristics. Further, investigation was processed to determine the optimal culture conditions degrading fenitrothion in NB medium by using the NFo1 strain. As results, the cultural conditions determined for temperature, initial pH and inoculum for the optimum growth of the strain and degradation of fenitrothion, which has a exact co-relationship between both of them, were $35^{\circ}C$, 7.5 and 1.5 at $OD_{660}$ value, respectively. In this conditions, fenitrothion could be degraded within 5 days over 90% at the high concentrations of fenitrothion, upto 200 mg/L.