• Title/Summary/Keyword: microflora

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Effect of Lentinus edodes on the Growth of Intestinal Lactic Acid Bacteria

  • Bae, Eun-Ah;Kim, Dong-Hyun;Han, Myung-Joo
    • Archives of Pharmacal Research
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    • v.20 no.5
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    • pp.443-447
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    • 1997
  • As the growth factor of lactic acid bacteria, LD (trehalose) was isolated from Lentinus edode5 by using silica gel column chromatography. LD induced the growth of Bifidobacteria breve and Lactobacillus brevis, which were isolated from human feces. LD selectively induced the growth of lactic acid bacteria among total microflora. When total intestinal microflora were cultured in the medium containing LD, it stimulated the growth of lactic acid bacteria and inhibited harmful enzymes, ${\beta}$-glucosidase, ${\beta}$-glucuronidase, and tryptophanase, of intestinal bacteria. LM, which was a monosaccharide from L. edooles, induced the growth of lactic acid bacteria but it seems to be invaluable in vivo. LH isolated from L. edodes by Sephadex G-100 column chromatography was not effective for the growth of lactic acid bacteria.

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Dietary Regulations of the Intestinal Barrier Function at Weaning

  • Bosi, Paolo;Gremokolini, Cyrien;Trevisi, Paolo
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.4
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    • pp.596-608
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    • 2003
  • Weaning is a complex phase when the mammal suffers the action of different stressors that contribute to negatively affect the efficiency of the intestinal mucosa and of the whole local integrated system, that acts as barrier against any nocuous agent. The components of this barrier are mechanical, chemical, and bacteriological; immunological and not. The development of contact with a saprophyte microflora and the maintenance of feed intake after the interruption of motherly nutrition are essential for the maturation of an equilibrated local immune function and for a functional integrity of villi. Opportunities and limits of some dietary strategies that can contribute to reduce negative effects of weaning on health and performance are discussed. Knowledges on the possible mechanism of action of probiotics are upgraded, particularly for their supposed role in the balance between different immune functions (effectory/regulatory). Some tools to control pathogen microflora are reviewed (acids, herbs, immunoglobulin sources) and practical feeding systems are proposed.

Changes of Microflora in Traditional Kanjang by Fermentation Jar (담금용기에 따른 재래식 간장의 미생물 변화)

  • 정혜정
    • Culinary science and hospitality research
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    • v.6 no.3
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    • pp.357-371
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    • 2000
  • Changes of Microflora, enzyme activity, and contents of several taste compounds in traditional Kanjang(Korean Soy Sauce) with the variation of fermentation jars and Meju(fermented soybean brick)concentration were studied. Substitution possibilities of clay jar with glass jar and improvement possibilities of taste and nutritional value with increased Meju concentration were examined. (1) Aerobic bacteria and lactic acid bacteria were enumerated during fermentation and ripening period. But yeast and molds were hardly isolated. The number of viable cells showed small changes during fermentation and ripening period. (2) Isolated Aerobic bacteria were identified as Bacillus subtilis, Bacillus lichenformis, and lactic acid bacteria were identified as Lactlbacillus lactis lactis and lactobacillus brevis. (3) The activities of $\alpha$-amylase and $\beta$-amylase increased during fermentation period, but did not show any trend by fermentation jars or Meju concentrations. Protense activity showed small difference among four samples, and increased during the fermentation period.

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The Functions of Lactic Acid Bacteria in Colon Cancer Prevention (결장암 예방에 대한 유산균의 기능)

  • Jeon, Woo-Min
    • Journal of Dairy Science and Biotechnology
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    • v.29 no.1
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    • pp.55-58
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    • 2011
  • Certain lactic acid bacteria have anti-tumor activity, especially colon cancer. The fermented milk products containing that kind of lactic acid bacteria have to be recommended for human health as excellent health functional foods. This paper have been classified by 5 regions on the functions of lactic acid bacteria related to prevention of colon cancer. 1) Enhancing of host's immune response; Production of cytokines. 2) Binding and degradation of potential carcinogens; Binding and degradation of mutagenicity. 3) The changes of intestinal microflora and production of antitumorigenic or antimutagenic compounds; Production of azoxymethane. 4) Alteration of the metabolic activity of intestinal microflora; Decrease of harmful enzymes in intestinal tract. 5) Alteration of physicochemical conditions in the colon; Decrease of pH and bile acids contents.

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Effects of Edible Herbs on the Growth of In Vitro Intestinal Microorganisms (산채류가 장내세균의 In Vitro 생육에 미치는 영향)

  • 한복진
    • Journal of Nutrition and Health
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    • v.27 no.7
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    • pp.717-728
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    • 1994
  • This study was aimed to screen edible herbs which control the composition of intestinal microflora. With in vitro experiments, we screened the water or ethanol extracts of about 60 edible herbs and wild plants in terms of the inhibition activity on the growth of the harmful Clostridium perfringens and growth promoting activity for the beneficial Bifidobacteria. The water extracts of mugwort and small water dropwort inhibited the growth of Cl.perfringens both in agar diffusion method and broth culture. On the other hand, the water extracts of petasites, mugwort, yellow day-lily and bitter cress have shown the promotion effect on the growth of Bifidobacterium longum. In the culture test using human feces as starter, the extracts of the above selected herbs increased the population of Bifidobacteria and Lactobacillus while they reduced the numbers of Cl.perfringens and E.coli.

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The Influence of Dietary Fiber on Intestinal Environment in Rats Controlled by Food Restriction (식이섬유 급원 첨가하에서의 식이제한이 흰주의 장내 환경에 미치는 영향)

  • 강어진
    • The Korean Journal of Food And Nutrition
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    • v.11 no.4
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    • pp.460-465
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    • 1998
  • The effects of food restriction on the fecal microflora, moisture, pH, indole, ${\beta}$-glucosidas, and ${\beta}$-glucuronidase in rate were studied for 4 weeks. Four groups of rats for feeding was allocated to the following experimental trials : (1) control containing 1% cellulose, (2) control with food restriction, (3) treatment of diet containing butterbur, (4) treatment of butterbur combined with diet restriction. Treatment of butterbur combined food restriction significantly (p<0.05) reduced the growth of Bacteroides, Peptococus, Streptococcus, Staphylococcus, and Escherchia coli, respectively. No remarkable changes in the ${\beta}$-glucosidase and ${\beta}$-glucuronidase activities were observed but indole content significanlty decreased. Based on these results, treatment of butterbur combined with diet restriction in rats had a significantly effect for preventing the growth of those pathogenic microorganims.

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Effects of the Water Extract of Akebia (Akebia quinata Edcaisne) on the Growth of Clotridium perfringens and Some Intestinal Microorganisms (목통 (Akebia quinata Decaisne)의 물추출물이 Clostridium perfringens 및 주요 장내미생물의 생육에 미치는 영향)

  • 한복진;우상규;신현경
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.633-640
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    • 1995
  • As a result of screening the medicinal herbs which selectively control human intestinal microflora, water extract of Akebia quinata Decaisne was proved to have a strong inhibitory activity against the growth of Clostridium pefringens, a major harmful intestinal bacterium. The anti-bacte-rial activity was stable under the thermal treatment at 100$\circ$C for 120 min and in a range of pH 1 to 11. In addition, the water extract of Akebia quinata Decaisne showed the antibacterial activities against five different strains of Clostridia including C. perfringens. On the contrary, the extract did not inhibit the growths of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis. The extract, however, suppressed markedly the growth of Bacteroides fragilis and Staphylococcus aureus in vitro. Alike in the mixed culture inoculated with human feces as starter, in vivo tests using rats showed that the extract tends to increase the numbers of Bifidobacteria and Lactobacilli in the intestinal microflora of rats, whereas those of Clostridia were attenuated.

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Effects of Dietary Fructan on Cecal Enzyme Activities in Rats

  • Kang, Soon-Ah;Chun, Uck-Han;Jang, Ki-Hyo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.582-586
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    • 2005
  • In this Study, we have attempted to determine the effects of dietary fructose polymers (fructan), high molecular-weight ${\beta}-(2,6)-linked$ levan, and low-molecular-weight ${\beta}-(2,1)-linked$ inulin, on two intestinal enzymes $({\beta}-glucuronidase\;and\;{\beta}-glucosidase)$. As a preliminary experiment, when intestinal microflora were cultured in anaerobic media harboring levan or its oligosaccharides, bacterial cell growth was observed in the levanoligosaccharide-supplemented media, but not in the levan-supplemented media, indicating that levan's size is important for the utilization by intestinal bacteria of levan as an energy Source. In our animal study, the intake of a levan-rich diet was determined to significantly attenuate the activity of the harmful enzyme $({\beta}-glucuronidase$, but d id not affect the activity of ${\beta}-glucosidase$.

Studies on the Selection and the Identification of Bacillus subtilis for Fermentation of Soybean (大豆醱酵를 위한 Bacillus subtilis 菌株의 純粹分離에 관한 연구)

  • Hur, Yun Haeng
    • Journal of Environmental Health Sciences
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    • v.12 no.2
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    • pp.67-74
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    • 1986
  • The study was carried out to investigate for the property of Bacillus strains, on the native growthed microflora in Korean native soybean paste, and Bacillus strains of the high enzyme producing, were selected and identificated, from the microflora, that is, identificated Bacillus strains beared resemblance to B. subtills, on the colony, appearance was pellicle, surface's spreading, color creamy-thin browned, colony elevation flated, and edge lobated, the identfficated B. subtills strain named for the B. subtilis SCF. For the protease activity of B. subtilis SCF, according to the variation with pH, the pH stability was pH 7~8, and on the its protease activity, optimum temperature was 40$\circ$C, on the other hand, temperature of the highest stability of the protease was 50$\circ$C.

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