• 대한기계학회논문집A
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• 제32권10호
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• pp.831-835
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• 2008

We present a continuous electrical cell lysis chip, using a DC bias voltage to generate the focused high electric field for cell lysis as well as the electroosmotic flow for cell transport. The previous cell lysis chips apply an AC voltage between micro-gap electrodes for cell lysis and use pumps or valves for cell transport. The present DC chip generates high electrical field by reducing the width of the channel between a DC electrode pair, while the previous AC chips reducing the gap between an AC electrode pair. The present chip performs continuous cell pumping without using additional flow source, while the previous chips need additional pumps or valves for the discontinuous cell loading and unloading in the lysis chambers. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present chip generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100% lysis rate of Red Blood Cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of $30{\mu}m/s{\pm}9{\mu}m/s$. In conclusion, the present chip is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2108-2112
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• 2007

The air and water flow distribution are experimentally studied for a round header-ten microchannel tube configuration. Three different inlet orientations (parallel, side, normal) were investigated. Tests were conducted with downward flow configuration for the mass flux from 70 to 130 kg/$m^2s$, quality from 0.2 to 0.6, non-dimensional protrusion depth (h/D) from 0.0 to 0.5. It is shown that, for almost all the test conditions, normal inlet yielded the best flow distribution, followed by side and parallel inlet. Possible reasoning is provided using flow visualization results.

• 전기학회논문지
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• 제56권3호
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• pp.561-565
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• 2007

We propose glass and PDMS (polydimethylsiloxane) chips for DNA amplification with continuous-flow PCR (polymerase chain reaction). The PDMS microchannel was fabricated using a negative molding method for sample injection. Three heaters and sensors of ITO (indium-tin-oxide) thin films were fabricated on glass chip. ITO heaters and sensors were calibrated accurately for the temperature control of the liquid flow. ITO heater generated stable heat versus applied power. ITO sensor resistance was changed linearly versus temperature increase as a RTD (resistance temperature detector) sensor. As a result, we enable precision temperature control of continuous-flow PCR chip. Using the continuous-flow PCR chip DNA plasmid pKS-GFP 720 bp was successfully amplified.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2003년도 추계학술대회 논문집
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• pp.75-78
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• 2003

An interfacial instability has recently been observed for the DC- and AC-powered electroosmotic flows of the two miscible electrolyte layers having different concentrations in microchannels. It is rather contrary to our common belief that the flow inside a microchannel is generally stable due to the dominant role of the viscous damping. In this work, we visualized the electroosmotic flow inside a T-channel to validate the numerical predictions. It is clearly shown that the strong vortices (which characterize the interface shapes) are generated at the interface of the two fluids, as was predicted in the numerical analysis.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2004년도 Proceedings of 2004 Korea-Japan Joint Seminar on Particle Image Velocimetry
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• pp.141-145
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• 2004

Visualization and PIV measurements of the symmetrical recirculation flow in a nanoliter-sized droplet have been performed using the micro PIV system. The airflow sweeps over the nanoliter-sized liquid droplet fixed in a microchannel and the frictional force drags the liquid on the round interface, which causes the symmetrical recirculation flow in the droplet. The internal recirculation flow in the droplet has been visualized and measured successfully. The results of micro PIV measurement show the maximum speed of the recirculation flow is up to 10 mm/s. The high-speed recirculation can enhance a stirring effect and generate strong shear in the droplet, resulting in acceleration of mixing.

• Korean Chemical Engineering Research
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• 제43권1호
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• pp.92-97
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• 2005

본 연구에서는 음성 감광제를 이용하여 모형을 제작하고, PDMS(polydimethylsiloxane)로 본을 뜬 후에 유리와 접합시켜 마이크로채널을 제작하였다. 특히 PDMS의 접촉각 변화에 따른 마이크로채널에서 유체의 속도변화를 측정하기 위하여, PDMS의 표면을 플라즈마를 이용하여 처리하였다. 표면처리된 PDMS의 접촉각은 $19^{\circ}$, $46^{\circ}$ 그리고 $69^{\circ}$였으며, 미처리된 PDMS의 접촉각은 $105^{\circ}$였다. 표면처리된 PDMS와 플라즈마 처리를 하지 않은 PDMS에 대하여 외부전압을 변화시켜서 마이크로채널에서의 유체의 속도를 측정하였다. 그 결과 동일한 접촉각을 갖는 PDMS에 대하여 외부전압을 변경시켰을 때, 외부전압이 증가할수록 유체의 속도가 비선형적으로 증가하였다. 이는 외부전압이 증가할수록 계면에서의 전하밀도가 증가하게 되고, 이로 인하여 전기이중층이 압축되어 표면전위가 증가하며, 따라서 제타전위의 값이 증가하기 때문인 것으로 해석된다. 또한, 동일한 외부전압에서 PDMS의 접촉각이 가장 작은 $19^{\circ}$일 때 유체의 속도가 가장 빠르게 나타났다. 이는 유체와 PDMS의 부착 정도에 따라 전기이중층 두께가 달라지고, 이러한 두께변화가 결과적으로 동일한 외부전압에서 접촉각의 크기에 따라 유체의 속도차이를 가져오는 것으로 사료된다.

• 대한기계학회논문집B
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• 제32권2호
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• pp.125-132
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• 2008

The bubble dynamics and heat transfer associated with nucleate boiling in parallel microchannels is studied numerically by solving the equations governing conservation of mass, momentum and energy in the liquid and vapor phases. The liquid-vapor interface is tracked by a level set method which is modified to include the effects of phase change at the interface and contact angle at the wall. Also, the reversible flow observed during flow boiling in parallel microchannels has been investigated. Based on the numerical results, the effects of contact angle, wall superheat and the number of channels on the bubble growth and reversible flow are quantified.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2002년도 추계학술대회 논문집
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• pp.51-52
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• 2002

Electro-osmotic flow in a PDMS microchannel of $66{\mu}m\;\times\;200{\mu}m\;\times\;3cm$ has been investigated using a micro PIV system. The field of view was $1056{\mu}m\;\times\;200{\mu}m$ and instantaneous velocity fields were obtained using two-frame cross-correlation method with $64\;\times\;64\;pixels^2$ interrogation window. In this study, we focused on the effect of applied electric field on the variation of internal flow with varying the electric field and seeding particles. The electro-osmotic flow shows a flat velocity profile and the mean velocity is proportional to the applied electric field.

• 대한기계학회논문집B
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• 제34권1호
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• pp.9-18
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• 2010

2 상 유동 압력강하에 대한 모델은 균질유동모델과 분리유동모델 두 가지가 있다. 많은 선행 연구자들은 마이크로채널에서의 2 상 유동 압력강하에 대한 상관식을 제시하였고, 대부분은 분리유동모델에 해당하는 Lockhart- $Martinelli^{(27)}$의 수정된 상관식에 기초하고 있다. 본 연구에서는 사각 마이크로채널에서의 압력강하에 대한 연구를 위해서 액상의 물과 기상의 질소를 사용하여 사각 마이크로채널에서의 실험을 수행하였다. 2 상 마찰 압력강하는 2 상 유동양식에 큰 연관성을 가지고 있는 결과를 확인할 수 있었다. 6 가지의 2 상 점성 모델을 포함한 균질유동 모델 ($Owen^{(21)}$'s, $MacAdams^{(22)}$'s, Cicchitti et ${al.}^{(23)}$'s, ${al.,}^{(24)}$ Beattie and ${Whalley,}^{(25)}$ Lin et ${al.}^{(26)}$)과 6 가지의 분리유동 모델 (Lockhart and $Martinelli,^{(27)}$ ${Chisholm,}^{(31)}$ Zhang et ${al.,}^{(15)}$ Lee and ${Lee,}^{(5)}$ Moriyama and ${Inue,}^{(4)}$ Qu and $Mudawar^{(8)}$)에 대한 평가를 실험결과와 비교를 통해 수행하였다. 가장 우수한 2 상 점성 모델은 Beattie and Whalley 의 모델이었고, 가장 우수한 분리유동 모델은 Qu and Mudawar 의 상관식이였다. 균질유동모델과 분리유동모델 모두에 대해서 2 상 유동양식에 종속성을 나타내었다. 그러므로, 본 연구에서는 2 상 유동 양식에 기초한 새로운 상관식을 균질유동모델과 분리유동모델에 대해 각각을 제시하였다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3187-3190
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• 2007

Elastin-Like polypeptide (ELP) composed of elastin-based repeating units is an artificial biomaterial which is biocompatible and non-immunogenic. ELP shows a characteristic inverse phase transition between hydrophobic and hydrophilic phase by external stimuli such as salt, pH and temperature. In this study, ELP coated PS (polystyrene) beads are packed in tubing and the thermo -responsive flow characteristics of the packed bed are investigated. Preliminary test results show that the control of the fluid flow can be achieved by using the temperature driven phase transition effect of the ELP coated beads in a microchannel.