• Title/Summary/Keyword: microbiology

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Nucleotide Sequence of 16S rRNA Gene from Streptomyces melanosporofaciens 7489

  • LEE, DONG-SUN;SUNG-OUI SUH;SEON-KAP HWANG;TAEG-KYU KWON;TAE-HO KIM;WOO-CHANG SHIN;SOON-DUCK HONG
    • Journal of Microbiology and Biotechnology
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    • v.6 no.5
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    • pp.364-365
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    • 1996

  • A region encoding the 16S rRNA was cloned by PCR from Streptomyces melanosporofaciens 7489 and sequenced by the chain-termination dideoxy sequencing method. A phylogenetic tree constructed by sequence alignment of 24 Streptomyces species suggests that there is little evolutionary distance between this strain and Streptomyces rimosus.

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Co-infection of Giardia intestinalis and Cyclospora cayetanensis in an Immunocompetent Patient with Prolonged Diarrhea: Case Report

  • Koru Ozgur;Araz Engin;Inci Askin;Tanyuksel Mehmet
    • Journal of Microbiology
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    • v.44 no.3
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    • pp.360-362
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    • 2006

  • Cyclospora cayetanensis is an agent of emerging infectious disease, and a recognized cause of diarrhea in some patients. Also, the flagellated protozoan, Giardia intestinalis, induces a diarrheal illness of the small intestine. Cases of cyclosporiasis are frequently missed, primarily due to the fact that the parasite can be quite difficult to detect in human fecal samples, despite an increasing amount of data regarding this parasite. On the other hand, G. intestinalis can be readily recognized via the microscopic visualization of its trophozoite or cyst forms in stained preparations or unstained wet mounts. In this report, we describe an uncommon case of co-infection with G. intestinalis and C. cayetanensis in an immunocompetent patient with prolonged diarrhea, living in a non-tropical region of Turkey.

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Effect of Nursing Students' Learning Motivation in Microbiology Lecture involved in Laboratory Based on the ARCS Model (ARCS모형에 근거하여 실습을 병행한 미생물학수업이 간호대학생의 학습동기에 미치는 효과)

  • Kim, Bo-Hwan;Hyong, Hee-Kyoung
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.6
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    • pp.1425-1434
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    • 2014

  • The purpose of this study was tried to identify the effect of nursing students' learning motivation in microbiology through microbiology laboratory practice based on the Keller's ARCS model. In order to achieve this research, this study was designed a quasi-experimental pre-post tests control group. Experimental group received a microbiology theory and practice based on ARCS model and control group received microbiology theory only. To identify the microbiology learning motivation effect to nursing student, we measured learning motivation by Keller's ARCS model that consisted of attention, relevance, confidence, and satisfaction. The major results of the experimental group showed significantly higher level of total learning motivation and all four subcategories compared to control group. Based upon the above results, microbiology laboratory practice might be beneficial for the nursing students to motivate microbiology learning.

  • https://doi.org/10.13000/JFMSE.2014.26.6.1425 인용 PDF KSCI

Bacteriocinogenic Potential of Newly Isolated Strains of Enterococcus faecium and Enterococcus faecalis from Dairy Products of Pakistan

  • Javed, Imran;Ahmed, Safia;Ali, Muhammad Ishtiaq;Ahmad, Bashir;Ghumro, Pir Bux;Hameed, Abdul;Chaudry, Ghulam Jilani
    • Journal of Microbiology and Biotechnology
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    • v.20 no.1
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    • pp.153-160
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    • 2010

  • The present study was carried out for the isolation of bacteriocin-producing enterococci from indigenous sources. Gram-positive enterococci are known for having the ability to produce enterocins with good antimicrobial potential. A total of 34 strains were isolated from processed dairy products of Pakistan and seven out of them were found to be member of genus Enterococcus on selective enumeration. Biochemical and molecular characterization revealed that four of these isolates (IJ-03, IJ-07, IJ-11, and IJ-12) were Enterococcus faecalis and three (IJ-06, IJ-21, and IJ-31) were Enterococcus faecium. Local processed cheese was the source of all enterococcal isolates, except E. faecium IJ-21 and IJ-31, which were isolated from indigenous yoghurt and butter samples, respectively. Bacterial isolates were sensitive to commonly used antibiotics except methicillin and kanamycin. They also lacked critical virulence determinants, mainly cytolysin (cyl), gelatinase (gel), enterococcal surface protein (esp), and vancomycin resistance (vanA and vanB). Polymerase chain reaction amplification identified that enterocin A and P genes were present in the genome of E. faecium IJ-06 and IJ-21, whereas the E. faecium IJ-31 genome showed only enterocin P genes. No amplification was observed for genes that corresponded with the enterocins 31, AS-48, L50A, and L50B, and ent 1071A and 1071B. There were no signals of amplification found for E. faecalis IJ-11, indicating that the antimicrobial activity was because of an enterocin different from those checked by PCR. Hence, the indigenous bacterial isolates have great potential for bacteriocin production and they had antibacterial activity against a variety of closely related species.

  • https://doi.org/10.4014/jmb.0904.04024 인용 PDF KSCI