• The Journal of the Korean Society for Microbiology
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• v.35 no.5_6
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• pp.366-366
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• 2000

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.230.4-230
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• 2005

• Journal of Microbiology and Biotechnology
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• v.4 no.3
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• pp.159-164
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• 1994

Myceliuml growth and spore formation of Streptomyces albidoflavus SMF301 in submerged culture were compared with the metabolism of cysteine. Cysteine added to the culture was metabolized by cysteine desulfhydrase (EC 4.4.1.1.) to produce ammonium ions, hydrogen sulfide, and pyruvate. The redox potential of the culture broth was lowered immediately as the result of the metabolism of cysteine, which caused a lag period of mycelium growth. However enhanced activities of pyruvate dehydrogenase and a-ketoglutarate dehydrogenase were confirmed in the culture containing cysteine, indicating that pyruvate was utilized to support further mycelium growth.

• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.129-134
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• 1992

Segregational instability of a recombinant plasmid, pDML6, encoding extracellular $\beta$-lactamase in Streptomyces lividans PD6 was characterized by growth kinetic analysis. The quantitative determination of the plasmid harbored in the mycelia was evaluated with mycelia fragmented mechanically, and also with colonies regenerated from protoplasts. Conditions for the formation of protoplasts and regeneration of protoplasts were established. The maximal specific growth rates of the host strain and the plasmid-harboring strain in a chemically defined medium without selection pressure were the same. The probability of plasmid loss from the harbouring cells was higher at higher growth rates. Mathematical models for the prediction of cell growth, substrate uptake, and accumulation of the cloned gene product were developed.

• Proceedings of the Microbiological Society of Korea Conference
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• 2009.05a
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• pp.70-71
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• 2009

• Proceedings of the Microbiological Society of Korea Conference
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• 2009.05a
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• pp.163-164
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• 2009

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.116.1-116.1
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• 2006

• Proceedings of the Korean Biophysical Society Conference
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• 2001.06a
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• pp.64-64
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• 2001

Escherichia coli has developed soxRS regulon to defend against toxicity of superoxide radical. SoxR, superoxide sensor, is oxidized by superoxide-generating agents or nitric oxide and oxidized SoxR activates the transcription of soxS gene. In order to find out the trans-acting factors regulating SoxR activity in vivo, soxS：：lacZ single copy operon fusion construct was prepared and random Tn10 insertional mutatons were performed.(omitted)

• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1218-1223
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• 2012

${\varepsilon}$-Poly-$_L$-lysine (${\varepsilon}$-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of $_L$-lysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce ${\varepsilon}$-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more ${\varepsilon}$-PL than those immobilized on synthetic sponge, and sugarcane bagasse. Moreover, loofah sponge supported the maximum biomass. Hence, loofah sponge was chosen for cell immobilization. Meanwhile, the ion-exchange resin D152 was employed for ISA. The loofah sponge-immobilized cells produced $0.54{\pm}0.1g/l$ ${\varepsilon}$-PL, which significantly increased to $3.64{\pm}0.32g/l$ after combining with ISA through the addition of resin bags. The free cells with ISA using the dispersed resin yielded $2.73{\pm}0.26g/l$ of ${\varepsilon}$-PL, an increase from $0.82{\pm}0.08g/l$. These data illustrate that the proposed combination method improved production most significantly compared with either immobilization or ISA only. Moreover, the immobilized cells could be repeatedly used and an ${\varepsilon}$-PL total amount of $8.05{\pm}0.84g/l$ was obtained. The proposed combination method offers promising perspectives for ${\varepsilon}$-PL production.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.292-296
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• 2004