• 한국식품영양과학회지
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• 제31권2호
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• pp.216-220
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• 2002

부산시내 초등학교 4곳을 방문하여 급식소 환경 , 기계, 기구에 대해 HACCP 적용시 중점관리점을 파악하기 위하여 위생평가를 실시하였다. 환경에 대한 위해 요소분석 결과 위해도가 높은 지역과 낮은 지역이 확실히 드러났으며 위생에 특별한 주의를 기울여야 할 장소가 확인되었다. 위생평가시 조사한 21곳의 시료장소 중에서 급식소의 바닥과 스테인레스 스틸제재로 된 배수설비 표면에서 높은 수의 세균과 대장균 군이 관찰되었다. 또한 덤웨이터에서 가장 높은 수의 세균과 대장균군이 발견되었고 밥통, 칼, 도마, 고무장갑, 운반대, 비닐앞치마, 플라스틱 소쿠리, 프라이 팬, 냉장고내부에서도 높은 수의 미생물이 검출되었으며 특히 기구들을 건조시키는 공기건조대에서도 오염도가 심해 특별히 관리 해야되는 부분으로 확인되었다. 또한 급식소의 내부에서 사용되는 물건 중 스테인레스 스틸로 만들어진 운반대, 선반, 식판, 국통 등에서도 단위면적당 수천 마리의 미생물이 오염된 것으로 나타나 용기를 통한 식중독균의 오염이 우려되고 있다. 따라서 단체 급식소의 HACCP의 적용에 앞서 이들 장소에 대해 위생표준 관리기준(SSOP)에 의한 중점관리가 수행되어져야 하며 주기적인 미생물 검사를 실시하여 미생물의 오염상태를 점검해야 할 것이다. 또한 주방기구나 용기는 이물질을 먼저 제거하고 세척제를 사용하여 세척한 다음 소독제를 사용한 소독을 실시한 후 말려 재사용하는 방법 이 철저히 적용되어야 할 것이다.

• 한국수산과학회지
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• 제56권2호
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• pp.182-187
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• 2023

Fifteen samples of dried laver Pyropia tenera were collected from markets and processing plants in Korea for an assessment of their microbial and chemical hazards, in accordance with the Korean food code. The contamination levels of total viable bacteria, coliforms, Escherichia coli, and nine other pathogenic bacteria (Staphylococcus aureus, Salmonella spp., Vibrio parahaemolyticus, Clostridium perfringens, Listeria monocytogenes, Enterohemorrhagic Escherichia coli, Yersinia enterocolitica, Bacillus cereus, and Campylobacter jejuni) were evaluated The concentrations of heavy metals (lead, cadmium, total mercury, and total arsenic) and radioactive isotopes (¹³¹I, and ¹³⁴Cs+¹³⁷Cs) in the laver samples were also determined. The total viable count of bacteria was 2.62±0.80 (1.48-4.45) CFU/g. The contamination levels of lead, cadmium, total mercury, and total arsenic were 0.024±0.005 (0.018-0.035), 0.090±0.038 (0.041-0.146), 0.008±0.005 (0.003-0.018) and 1.315±0.372 (0.814-1.930) mg/kg, respectively. All samples tested negative for significant levels of radioactivity, the nine pathogenic bacteria, coliforms, and E. coli (<1.00 CFU/g). We assume that ensuring the microbiological and chemical safety of dried laver can increase the demand for its exportation. The present study may serve as a basis for microbiological and chemical hazard assessment of dried lavers.

• 한국식품영양과학회지
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• 제40권1호
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• pp.116-122
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• 2011

어린이집 유아 휴대 수저 및 수저집의 미생물 오염도를 분석하고, 가정과 어린이집에서 수저 및 수저집의 위생 안전성을 확보할 수 있는 세척 방법과 자외선 살균소독 시간을 제시하고자 유아 휴대 수저 36건 및 수저집 42건 등 총 78건을 실험하였다. 수저 36건 중 20건(55.6%), 지퍼형 수저집 20건 중 9건(45.0%), 플라스틱형 수저집 22건 중 13건(59.1%)에서 2.7 log CFU/$100cm^2$ 이상의 일반세균수가 검출되어 즉각적인 위생조치를 강구해야 하는 수준으로 조사되었다. 또한, 수저 36건 중 19건(52.8%), 지퍼형 수저집 20건 중 14건(70.0%), 플라스틱형 수저집 22건 중 14건(63.6%)에서 대장균군이 검출되어 어린이집 유아 휴대 수저 및 수저집에 대한 위생조치가 요구되며, 가장 높은 위해수준을 나타낸 지퍼형 수저집에 대한 각별한 주의가 필요한 것으로 판단되었다. 수저와 플라스틱형 수저집에서는 실험한 모든 식중독 미생물이 검출되지 않았으나, 지퍼형 수저집 20건 중 2건(10.0%)에서 Staph. aureus가 검출되었고, 3건(13.6%)에서 B. cereus가 검출되어 지퍼형 수저집이 식중독 미생물 오염에 가장 취약한 것으로 나타났다. 세척 방법에 따른 미생물 저감화 효과를 실험한 결과 수세미에 주방세제를 첨가하여 30초간 세척한 후 30초간 흐르는 수돗물로 세척했을 경우 수저, 지퍼형 및 플라스틱형 수저집 모두 대장균이 검출되지 않았다. 따라서 가정에서 수저 및 수저집을 1분 이상 깨끗이 세척, 건조시켜야 될 것으로 판단되었다. 자외선 살균소독 시간에 따른 미생물 저감화 효과를 실험한 결과 15분 살균시 대장균이 검출되지 않아 어린이집에서 수저 및 수저집을 15분 이상 자외선 살균소독기로 살균할 경우 대장균을 제어할 수 있을 것으로 판단되나, 각 어린이집의 자외선 살균소독기의 램프 출력량과 살균대상 수저집의 수량을 고려하여 각각의 제조사가 제시하는 자외선 살균소독기 권장살균시간 이상 수저 및 수저집을 살균하여 제공하여야할 것으로 판단된다.

• 한국식품위생안전성학회지
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• 제27권1호
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• pp.30-36
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• 2012

어린이집 유아 손의 미생물 오염도를 분석하고 손 씻기 교육의 미생물 저감화 효과를 실험하여 어린이집 유아 손의 위생 안전성을 확보하고자 어린이집 유아 59명을 대상으로 실험하였다. 일반세균수의 경우 평균 $3.72{\pm}0.38log\;CFU/hand$로 검출되었고, 위생지표미생물인 대장균군의 경우 남자어린이 18명 중 2명(11.1%)과 여자어린이 17명 중 3명(17.6%), 총 35명 중 5명(14.3%)에서 검출되어 어린이들에 대한 반복적인 손 씻기 지도가 필요한 것으로 판단되었다. $Staph.$ $aureus$는 35명 중 9명(25.7%), $B.$ $cereus$는 35명 중 16명(45.7%)에서 검출되었고 남자어린이의 손이 식중독 미생물 오염에 가장 취약한 것으로 나타났다. $Staph.$ $aureus$ 장독소는 9균주 중 $Staph.$ $aureus$ Hand-1 한 균주에서만 SEA 장독소가 검출되었으나 HBL과 NHE 장독소 중 한 가지 이상의 장독소를 생산하는 $B.$ $cereus$는 총 12균주(70.0%)로 나타나 어린이 손에 의한 교차오염 위험성이 상존한다고 판단되었다. 손 씻기 교육의 미생물 저감화 효과를 실험한 결과 교육을 실시한 그룹이 0.18 log CFU/hand 일반세균수가 더 저감화가 되었으나 저감화 정도가 매우 미약하여 손에 부착되어 있는 세균을 완전히 제거하기 위해서는 손 씻기와 손 소독을 병행하며 지속적인 손 씻기 교육을 실시하여야할 것으로 판단되었다.

• 대한영양사협회학술지
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• 제12권2호
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• pp.157-171
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• 2006

This study was intended to suggest HACCP-based standard recipe for bellflower roots, classified as no thermal cooking process, served in a university foodservice operation. The time-temperature and microbial contamination level in each cooking step were analyzed. The temperatures of bellflower root, peeled garlic and green onion at receiving were at 13.8$\pm$2.8, 12.6$\pm$2.9 and 13.7$\pm$$0.8^\circC$ respectively, which were above the temperature limit. The time consumed for pre-preparation was up to 90 min at room temperature having high microbial growth potential. The levels of total plate counts (TPC) of bellflower root and garlic were over the limit of $10^6$ CFU/g as were the numbers of coliforms in bellflower roots. There were no microbial reductions in pre-preparation and cooking, which resulted in over $10^5$- $10^6$ CFU/g of TPC at service step. Two CCPs identified were washing/sanitation at pre-preparation and service steps. The control measures were washing/sanitation and temperature control. It was verified that CCPs for no cooking process developed in preceding studies were applicable for the microbiological food safety of this menu item. The HACCP-based standard recipe was developed to produce a quantity for 100 servings by observing the critical limits established for CCPs. These results suggest that the selection of proper provider is imperative to control the microbial contamination of raw materials at purchasing step. Also, the sanitary education program should be developed for the employees to understand and comply the HACCP plan and standard recipe.

• 동아시아식생활학회지
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• 제24권6호
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• pp.845-852
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• 2014

The quality of commercial sauce products was evaluated through pH, soluble solid content, salinity, water activity and microbial analyses. The pH of sauces was 2.38~5.30, soluble solids were between 6.03 and 71.67, and distributions of salt were 0.23~5.00% in 32 commercial sauce products. In addition, water activity of vinegar red pepper sauce and spicy soft tofu stew stock were determined 0.773 and 0.988, respectively. Yeast, mold, Staphylococcus aureus, E. coli and coliform were not detected in any sauces. Higher level of total viable cells (TVC) resulted in pH over 4.2. TVC of shelf-stable sauces was 1.0~3.6 log CFU/g. TVC of seven sauce products was classified as non-potentially hazardous foods by temperature controlled for safety standard (TCS), even though levels were over acceptable guidelines of the USDA (3 log CFU/g). These results indicate that the standard and classification of commercial sauce products should be modified and controlled strictly.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2000년도 International Meeting 2000
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• pp.94-101
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• 2000

We investigated the viral contamination of water environment including tap water in Korea. River water used for source water was analyzed about monthly between 1997 and 1999 over a period 26 months. A total of 22 tap water samples were collected in 10 sites in 2 urban areas between 1997 and 1998 over a 11 months. All samples were examined for infectious enteroviruses and adenoviruses by a cell culture technique followed by PCR amplification. To identify the recovered viruses from tap water, sequence analysis of PCR products was performed. Infectious viral particles were detected in river water all year round, ranging from 0.93 to 17.3 Most Probable Number of Infectious Unit (MPNIU) /100L. Tap water samples also contained infectious viral particles. The frequency of enteroviruses and adenoviruses in tap water were $50.0\%$ (11/22) and $36.7\%$ (8/22), respectively. Both enteroviruses and adenoviruses were detected in five tap water samples $(22.7\%)$. The level of viral contamination in tap water was quite high, ranging from 0.2 to 2.9 MPNIU/100L, far above the recommended virus level in drinking water set by the U.S. EPA. Poliovirus type 1 derived from vaccine was frequently detected and the remainder comprised coxsackievirus B type or echovirus type 6, which were causative agents of aseptic meningitis in Korea in 1997 and 1998, respectively. Several types of adenovirus were detected in tap water samples and some water samples were found to contain adenoviruses which were closely related to enteric adenovirus type 40 and 41. This stusy shows that surface water and tap water in Korea may be exposed to the risk of viral contamination, especially from recently recognized viruses and this constitutes a potential public health hazard.

• 한국식품조리과학회지
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• 제24권5호
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• pp.652-664
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• 2008

In this study, a Hazard Analysis Critical Control Point (HACCP) plan was developed for the sanitary mass production of commercial Korean rice cake products (Gaepidduk, Injulmi, and Julpyon). The microbiological properties of manufacturing flow were evaluated in order to develop the HACCP Plan. The moisture contents of the rice cakes ranged between 36.2${\sim}$55.3%, whereas the water activity of all samples ranged between 0.954${\sim}$1.0. Microorganisms testing was conducted during various phases of the product flow of Korean rice cake preparation, and included assessments of food equipment, work environment, and cooking employees on a small scale. During the manufacture of Injulmi, Julpyon and Gaepidduk, CCPs were purchasing & storage, steaming and cooling, molding, and holding in the A and B manufactories. At the critical limit of CCPs, storage was conducted below at $5^{\circ}C$ in soybean powder, oil, and paste with redbeans. The steaming process was conducted above at $99^{\circ}C$ for 40 min. Cooling and holding processes were conducted for 2 hours below at $15^{\circ}C$. The molding process included sanitary education for foodhandlers and training for operators. Thus, certain prerequisite programs had to be implemented prior to the implementation of the HACCP system. High levels of bacterial contamination were detected in the aprons worn to work by some employees. Additionally, periodic sanitary education for foodhandlers and training for operators or managers was required. Cross contamination by materials was expected at the place where materials were processed or stored.

• 한국축산식품학회지
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• 제38권6호
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• pp.1237-1245
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• 2018

In this study, the contamination levels of hygienic indicators and foodborne pathogens in retail meat products were investigated in relation to the various market factors including processing temperature, processing area, and market type. Ground beef samples (n=80) were purchased from 40 meat markets and investigated for microbiological quality. Beefs processed below $20^{\circ}C$ had significantly lower numbers of total coliforms (TC) than these processed over $20^{\circ}C$ (2.01 vs. 2.79 log CFU/g; p<0.05). Interestingly, separation of processing area did not affect the contamination levels. Remarkably, the contamination levels of hygienic indicator differ among market types, indicating that not only processing condition but distribution structure that is directly related with storage period could affect the final microbiological loads of the meat products. In addition, the prevalences of Listeria monocytogenes (a psychrotroph), Enterococcus faecium, and Enterococcus faecalis were 7.5% (6/80), 10.0% (8/80), and 20.0% (16/80), respectively, which is irrelevant to market factors except meat products from wholesale markets where no L. monocytogenes were found among 30 samples. The results of this study indicate that the contamination level of hygiene indicator and foodborne pathogens in retail beef is more related with processing temperature and storage period than other environmental factors.

• 급식외식위생학회지
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• 제3권1호
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• pp.38-44
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• 2022

This study investigated the microbiological hazards in the manufacturing processes of Naengmyeon. Sanitary indicative bacteria, such as aerobic plate counts and coliforms as well as pathogenic bacteria, were examined from raw materials, manufacturing processes, working area, 17 utensils and equipment. The aerobic plate counts for raw materials and arrowroot starch estimated as 0.77±0.68~5.02±0.28 and 5.02±0.28 log CFU/g, respectively. Coliforms were detected from wheat flour, buckwheat flour, and potato starch. Staphylococcus aureus was detected to be 0.61±1.06 log CFU/g in wheat flour and 0.20±0.35 log CFU/g in buckwheat flour. During the manufacturing process, aerobic plate counts for kneading process were 4.54±0.34 log CFU/g. But after the press out and heat process, contamination of aerobic plate counts and coliforms decreased and remained at a low level until the release process. Aerobic plate counts before washing disinfection of screw were 3.28±0.62 log CFU/100 cm², the level of which was high in utensils and equipment that had contact with employees or water. These results represent not only an important indicator for the hygienic level but also a scientific basis for analyzing biological hazards, which lead to the introduction of HACCP for the production of safe and hygienic cold noodles processed by manufacturers.