• Title/Summary/Keyword: microbiological distribution

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Distribution of Azotobacter in Rhizosphere and Sea (Rhizosphere와 해수에 있어서의 Azotobacter의 분포)

  • 홍순우;최영길
    • Korean Journal of Microbiology
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    • v.12 no.1
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    • pp.15-24
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    • 1974
  • This experiment has been carried out with a view to elucidating the distribution of Azotobacter and their population size in rhizosphere and sea and designed ro compare the results with some environmental factors. Results of the experiment are summarized as follows: 1) It was observed that the population sizes of Azotobacter were decided upon the moisture content of soil and that the soil pH was one of the most impertant factors influencing the distribution of Azotobacter. 2)Population sizes of Azotobacter in rhizosphere were changed in accordance with the kinds of vegetation on soil: The rhizosphere where bamboo, corn, legume, and oak inhabit showed the largest population size of Azotobacter. On the other hand, rhizosphere of ginseng revealed no Azotobacter. However, the largest population of general fungi were measured at the rhizosphere. 3)Comparing the population sizes of general microbes in rhizosphere with those of non-rhizosphere, the population sizes of microbes in rhizosphere are larger than those of non-rhizosphere. 4)In coastal environments, population sizes of Azotobacter in surface water of sea are similar to those of the soil(mud) of tidal land. But the sizes are generally smaller than those of terrestrial soils.

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Microbial Distribution in Jinhae-bay (I) (진해만의 미생물분포 I)

  • 최영길
    • Korean Journal of Microbiology
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    • v.19 no.2
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    • pp.45-51
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    • 1981
  • The physico-chemical and biological factors of coastal sea water were measured bimonthly from 1976 to 1979 for elucidating the relationship between microbial distribution and environmental factors at Masan and Jinhae bay. The experimental results are summarized as followings : 1) The polulation size of bactriz in sea water were increasing as the water temperature increased, and that was higher at station 2 and 3 than at station 1. The number of fungi showed the highest value on July on bottom. The population size of yeast showed no seasonal variation and also showed a relation with the geographic distance. 2) The correlationship between microbial distribution and environmental factors showed little coefficiency in surface water. And the other hand, at bottom water, between general bacteria and water temperature and dissolved oxygen, and between yeast and salinity, there were relatively high coefficiecy.

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Distribution of heterotrophic bacteria and physico-chemical characteristics of sediments in Kum river estuary (금강 하구 퇴적토의 이화학적 성질과 종속영양세균의 분포에 관하여)

  • 이건형;아영칠;홍순우
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.308-316
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    • 1986
  • Vertical distribution of heterotrophic bacteria and physico-chemical characteristics were measuted in Kum River estuarine sediments. And interrelationship between heterotrophic bacterka and environmental factors was also studied. The type of sediment of Site 1 was silty clay, and sand at Site 2. Annual pH ranges were between 7.1 and 7.7 in the clay type sediment (Site 1) and 6.9-7.2 in the sand type sediment (Site 2). It was shown that organic matter contents were higher in the clay type sediment than those of sand type sediment. Redox potential values of sediments were decreased rapidly with depth at Site 1, but those of Sete 2 showed vertical fluctuation. Nitrogens(ammonia+amino acid-N, nitrate-N, nitrite-N) and phosphate in the clay type sediment showed higher values than those of sand type sediment. Annual distribution of heterotrophic bacteria were ranged $6.71{\times}10^4$ cells/g dry wt. $-2.50{\times}10^6$ cells/g dry wt. In the clay type sediment and $2.67{\times}10^3$ cells/g dry wt. $-1.94{\times}10^6$ cells/g dry wt. in the sand type sediment. Distribution of proteolytic, lipolytic, and amylolytic bacteria were decreased with the depth and the highest density was found in April and the lowest in January. Bacterial populations in sediments were closely correlated with such environmental factors as pH, redox potential, moisture content, organic matter contents, and inorganic nutrients such as nitrite-N and phosphate-P.

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Detection of Microbial Contamination in Commercial Berries

  • Cho, Kyu-Bong
    • Biomedical Science Letters
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    • v.23 no.4
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    • pp.333-338
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    • 2017
  • This study was performed to assess microbial contamination of Aronia melanocarpa, blueberry, raspberry, and cranberry sold in several markets. We investigated total aerobic bacteria and detected foodborne bacteria by multiplex PCR from Aronia melanocarpa, blueberry, raspberry, and cranberry. Total aerobic bacteria of each sample showed mean 3.54 log CFU/g for Aronia melanocarpa, mean 1.90 log CFU/g for blueberry, and mean 1.40 log CFU/g for raspberry, but not detected in cranberry. Specially, Aronia melanocarpa contained high total aerobic bacteria contamination among various berries and contamination level reached 4.17 log CFU/g in sample 5. To evaluate the effect of distribution conditions, we also investigated total aerobic bacteria of various berries. Total aerobic bacteria showed mean 2.89 log CFU/g for berries in refrigerated distribution and 1.40 log CFU/g in frozen distribution, but not in dry distribution. For assessment of foodborne bacteria contamination, we conducted PCR with multiplex primers of E. coli O157, S. aureus, B. cereus, V. parahaemolyticus, L. monocytogenes, Y. enterocolitica, Salmonella spp., Shigella spp. Among these foodborne bacteria, B. cereus was amplified in Aronia melanocarpa in sample 4 and blueberry in sample 1, 2, 3, and 5. The result of quantitative analysis of B. cereus contamination showed 4.08 log CFU/g of Aronia melanocarpa in sample 4 and higher contamination rate 4.07 log CFU/g of blueberry in sample 3. These results suggest that strict food safety control in harvest and distribution of various berries is necessary to prevent foodborne disease and improve microbiological safety.

Bioavailability and Comparative Pharmacokinetics of Two Enrofloxacin Formulations in Broiler Chickens (육계에서 경구투여시 enrofloxacin 제제에 따른 생체이용율 및 약물동태)

  • 윤효인;박승춘
    • Journal of Veterinary Clinics
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    • v.14 no.2
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    • pp.195-200
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    • 1997
  • In order to compare the pharmacokinetic profiles of enrofloxacin-HCL)ENFLX-HCL) and enrofloxacin-KOH (ENFLX-KOH) after oral administration in broiler chickens, the study was performed. The chickens used in this study weighed $1.82 {\pm}0.2 kg$ and clinically healthy. The dose of intravenous and lral administration was 5 mg per kg of body weight as enrofloxacin. After intravenous injection of enrofloxacin, it showed two-compartment model with the rapid distribution phase and the slow elimination phase. The mean apparent volume of distribution (Vd) was 2.70 l/kg. The mean half-life of elimination and distribution showed 8.26 h and 0.44 h, respectively. The mean area under curve (AUC) was calculated as $19.7 {\mu} g{\cdot} h/ml$. After oral administration of ENFLX-HCL and ENFLX-KOH with a rate of dose 5 mg of enrofloxacin/kg of body weight, Both of the products were showed one-compartment model unlike that of i.v. enrofloxacin standard solution showed the mean bioavailability of 79.64% for the ENFLX-KOG and 86.24% for the ENFLX-HCL. The mean total body clearance of the former was 0.24 l/kg/h and the latter 0.42 l/kg/h. Both enorfloxacin formulations seemed to have good tissue distribution and penetration as indicated by large volume of distribution : 2.72 l/kg for the -KOH and 4.44 l/kg for the -HCL. With the results obtained in this study, ENFLX-HCL could be used in place of its salt form in chickens.

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Microbial Distribution in Refrigerated Beef (냉장 우육내의 미생물의 분포)

  • 정해만;조광필
    • Korean Journal of Microbiology
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    • v.29 no.3
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    • pp.195-198
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    • 1991
  • Isolation and identification of mesophilic and psychrotrophic bacteria distributed in Korean refrigerated beef were attempted. Total isolated colonies were 192, and identified as 5 genera and 10 species. Among them, mesophilic bacteria were Enterobacter aerogenes, E. agglomerans, Serratia liquefaciens, Proteus mirabilis, and "psychrotrophic" bacteria were Pseudomons fluorescens, P. putida, P. pickettii, P. mendocina, P. stutzeri, Alcaligenes faecalis. Dominant species was Serratia liquefaciens as mesophiles, and Pseudomonas putida as psychrotroph.chrotroph.

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Isolation and Identification of Detergents Degrading Bacteria in the Han River Downstream (서울시역 한강수계에서의 합성세제 분해세균의 분리 및 동정)

  • 배경숙;하영칠;홍순우
    • Korean Journal of Microbiology
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    • v.20 no.2
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    • pp.98-104
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    • 1982
  • Detergent-degrading bacteria isolated from the Han River downstream running through the center of Seoul area were identified. Samples were monthly taken from the surface waters at four designated sites from October, 1980 to August, 1981. Isolated strains were characteristically Gram-negative bacteria in all, and they were classified within 18 genera, 43 spceies. The dominant strains of detergent -degrading bacteria were within geneus Pseudomonas. On the regional distribution of detergent-degrading bacteria, Euterobacteriaceae and genus Aeromonas were suddenly increased at the Third Bridge of site 2 and the First Bridge of site 3, and others were almost the same regardless of sites.

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Evaluation of Biodegradation Characteristics of Haloacetic Acids by a Biofilm in a Drinking Water Distribution System (상수관망에서 생물막에 의한 Haloacetic Acids 생물분해 특성 평가)

  • Son, Hee-Jong;Kim, Do-Hwan;Han, Young-Rip;Choi, Young-Ik
    • Journal of Environmental Science International
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    • v.23 no.9
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    • pp.1635-1642
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    • 2014
  • Haloacetic acids (HAAs) concentrations have been observed to decreased at drinking water distribution system extremities. This decrease is associated with microbiological degradation by pipe wall biofilm. The objective of this study was to evaluate HAAs degradation in a drinking water system in the presence of a biofilm and to identify the factors that influence this degradation. Degradation of monochloroacetic acid (MCAA), dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA) was observed in a simulated distribution system. The results obtained showed that different parameters came into play simultaneously in the degradation of HAAs, including retention time, water temperature, biomass, and composition of organic matter. Seasonal variations had a major effect on HAAs degradation and biomass quantity (ATP concentration) was lower by 25% in the winter compared with the summer.

Seasonal Distribution and Characteristics of Heterotrophic Marine Bacteria in the Intertidal Zone Near Kunsan of Yellow Sea, Korea (군산부근 조간대에서의 해양종속영양 세균의 계절적 분포와 특성)

  • 이건형;김상진;이원호;이다미
    • Korean Journal of Microbiology
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    • v.28 no.4
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    • pp.331-336
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    • 1990
  • Annual distribution of hiterotrophic marine bacteria and seasonal characteristics were investigated in the intertidal waters and sediments in the vicinity of Kunsan of Yellow Sea, Korea. Annual distribution of heterotrophic marine bacteria ranged from $ 7.5*10^{2}$ to $1.1*10^{5}$ c.f.u./ml in water and from $1.6*10 ^{4}$ to $4.8*10^{6}$ c.f.u.per g dry sediments. As for the morphological distribution measured by epifluorescence microscopy, rod-form bacteria were distributed more than 74% of total observed bacteria during surveying periods. Average biovolume of sampled bacteria ranged from $3.19\;+-\;0.59*10^{-2}$ to $6.19\;+-\;0.76*10^{-2}$ $\mu$$m^{3}$ for coccus-form bacteria. Isolated bacteria showed various utilization of carbon sources such as glucose, maltose, lactose, xylose and arabinose, and showed tolerance at various range of salinity. It was isolated 82 genus in seawater and 114 genus in sediments. Dominant genera were Pseudomonas, Vibrio, Flavobacterium and Acinetobacter in seawater, and Pseudomonas, Acinetobacter, Vibrio, and Mycobacterium in sediments.

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A Study on simulation analysis of the microbe transport of air-born virus in hospital for microbiological safety (미생물 안전을 위한 병원건물의 환기설계에 따른 공기 감염균 확산에 관한 시뮬레이션 연구)

  • Choi, Sang-Gon
    • Journal of the Korea Safety Management & Science
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    • v.19 no.4
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    • pp.115-123
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    • 2017
  • Recently there is a growing interest in the airborne spread of virus. In particular, there is growing interest in secondary infection through the air in the hospital. The distribution of air-born virus depends on ventilation system installed in a hospital. In this study, simulations were carried out to predict the move of air-born virus by ventilation system at hospital. Simulation results showed that pressure distribution was -372.05Pa ~ -3.45 Pa at 1st floor incase of only used mechanical exhaust at bathroom, shower stall, storage, kitchen etc.. if ventilation switch from used mechanical exhaust to mechanical exhaust & mechanical supply. Simulation results showed that pressure distribution was -336.44Pa at stair hall < -0.2Pa at bathroom < mean 1.19Pa at other room. So simulation results showed that using all of the mechanical supply and mechanical exhaust was more effective then the mechanical exhaust for maintain the pressure distribution in hospital. It was also showed that when using the mechanical supply and mechanical exhaust more effectively prevention of air born virus diffusion.