• Title/Summary/Keyword: microbiological analysis

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Study of the Microbiological Limitation Standards Setting of Baking Processing by Confectionery (과자류의 굽기공정에 미생물학적 한계기준 설정에 관한 연구)

  • Lee, Ung-Soo;Kwon, Sang-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.8
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    • pp.5422-5429
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    • 2015
  • Main idea of this study is applying The HACCP (Hazard Analysis Critical Control Point) system to Confectionery. LB company, located in Gyeryong-si, Chungnam, provided main ingredients of confectionery, work facilities and workers between September 1, 2014 and January 15, 2015. The manufacturing process was made by Referring to the typical manufacturing process of confectionery manufacturers. Based on the microbiological hazard analysis, egg contained $6.2{\times}10^3CFU/g$ of bacteria, which has most amount of bacteria among agricultural materials. However, the Microbiological hazard analysis of the raw materials and after the baking process of confectionery showed a safe result. As a result, the microbiological hazard should be reduced by systematic cleaning, disinfection and improved sanitary education.

A treatment of drug resistant denture stomatitis by microbiological analysis and adjuvant therapy: a case report (미생물학적 분석과 보조요법을 이용한 내성을 가진 의치성구내염 치료)

  • Song, Young-Gyun
    • Journal of Dental Rehabilitation and Applied Science
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    • v.38 no.2
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    • pp.120-126
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    • 2022
  • In severe cases of denture stomatitis, antifungal drugs are sometimes ineffective. This case is a 72-year-old patient who was prescribed fluconazole for five months but had no improvement in symptoms. After re-diagnosis, medication was performed, and temporary dentures were fabricated for oral rehabilitation. This report describes that microbiological analysis and adjuvant therapy can be helpful, in case does not improve symptoms even with continuous medication of antifungal agent.

Guidelines for Microbiological Standards of Food in Foreign Countries (제외국의 식품안전관련 미생물 정량기준 가이드라인)

  • Lee, Mi-Seon;Woo, Gun-Jo;Park, Jong-Seok;Lee, Dong-Ha;Oh, Sang-Suk
    • Journal of Food Hygiene and Safety
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    • v.19 no.3
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    • pp.140-150
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    • 2004
  • It has been recognized that international food standards should be considered for a long time. The Codex Alimentarius programme initiated in the early 1960s has received valuable background documentation from the ICMSF (International Commission on Microbiological Specification for Foods) and also from ISO(the International Organization for Standardization). There has been a considerable move towards international harmonization of standards and methods of analysis. Many foreign countries recommend the guidelines for food microbiological standards quantitatively. To complement microbiological limits and tolerance levels for permissible number of defective samples, allowances should be made for sampling and other variations in laboratory methods. It may be necessary for guidelines of domestic food microbiological standards to be established so that the domestic food standards can be harmonized with foreign food standards. Food safety related microbiological guidelines of ICMSF, EU (European Union), UK (United Kingdom), China (Hong Kong) and Japan were reviewed and shown in examples.

Hazard Analysis of Packaged Meals (Dosirak) During Delivery (도시락 유통과정의 미생물적 품질관리를 위한 연구)

  • Shin, Sung-Won;Rew, Kyung;Kwak, Tong-Kyung
    • Journal of Food Hygiene and Safety
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    • v.5 no.3
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    • pp.85-98
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    • 1990
  • Microbiological quality of packaged meals (Dosirak) was assessed in three operations under the both conditions of actual food processing and simulated time-temperature. Time and temperature data indicated that all the phases after cooking were critical for microbiological quality control. Microbiological test results by simulated time-temperature conditions revealed that microbiological quality of packaged meals decreased as the storage time and temperature increase. Delivery practices without refrigeration in summer and hot-holding below $60^{\circ}C$ in supermarket were crucial in microbiological quality control.

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Isolation of an Isocarbophos-Degrading Strain of Arthrobacter sp. scl-2 and Identification of the Degradation Pathway

  • Li, Rong;Guo, Xinqiang;Chen, Kai;Zhu, Jianchun;Li, Shunpeng;Jiang, Jiandong
    • Journal of Microbiology and Biotechnology
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    • v.19 no.11
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    • pp.1439-1446
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    • 2009
  • Isocarbophos is a widely used organophosphorus insecticide that has caused environmental pollution in many areas. However, degradation of isocarbophos by pure cultures has not been extensively studied, and the degradation pathway has not been determined. In this paper, a highly effective isocarbophos-degrading strain, scl-2, was isolated from isocarbophos-polluted soil. The strain scl-2 was preliminarily identified as Arthrobacter sp. based on its morphological, physiological, and biochemical properties, as well as 16S rDNA analysis. The strain scl-2 could utilize isocarbophos as its sole source of carbon and phosphorus for growth. One hundred mg/l isocarbophos could be degraded to a non detectable level in 18 h by scl-2 in cell culture, and isofenphos-methyl, profenofos, and phosmet could also be degraded. During the degradation of isocarbophos, the metabolites isopropyl salicylate, salicylate, and gentisate were detected and identified based on MS/MS analysis and their retention times in HPLC. Transformation of gentisate to pyruvate and fumarate via maleylpyruvate and fumarylpyruvate was detected by assaying for the activities of gentisate 1,2-dioxygenase (GDO) and maleylpyruvate isomerase. Therefore, we have identified the degradation pathway of isocarbophos in Arthrobacter sp. scl-2 for the first time. This study highlights an important potential use of the strain scl-2 for the cleanup of environmental contamination by isocarbophos and presents a mechanism of isocarbophos metabolism.