• 한국농공학회:학술대회논문집
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• 한국농공학회 2005년도 학술발표논문집
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• pp.661-666
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• 2005

Wastewater reuse are exposed public health risk by pathogens. Therefore, this study was examined for microbial risk assessment after irrigation as treated wastewater in paddy rice plots. Five treatments were used: biofilter effluent, UV disinfected water, pond treatment, wetland treatment and conventional irrigation water. Risk assessment was calculated based on the beta-Poisson model by concentration of E. coli from 2003 to 2005. Monte-Carlo simulation (n=10,000) was used to estimate the risk characterization of uncertainty. The risk range was from $10^{-5}$ to $10^{-8}$ except biofilter effluent was $10^{-4}$ in June. The USEPA(1992) has recommended that risk of < $10^{-4}$ is acceptable level of safety for potable waters. In 2005, risk value was lower than 2003, 2004 because of the first irrigation for plowing water is lower E. coli concentration used tap water. It is shown that the first irrigation water quality was important for wastewater irrigation in paddy. UV disinfection and natural treatment used pond and wetland were thought to be an effective for wastewater reuse.

• 한국식품위생안전성학회지
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• 제33권4호
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• pp.248-254
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• 2018

본 연구에서 소비-섭취 시나리오와 온도-시간의 장염비브리오 생육모델을 활용하여 국내 생굴의 병원성 장염 비브리오균의 위해평가를 실시하였다. 장염 비브리오균의 오염 수준 및 병원성 인자 데이터를 활용하였으며, 국민건강영양조사와 농촌진흥청의 표준레시피를 활용하여 섭취량을 조사하였고 용량반응관계는 Beta-Poisson모델을 활용하였다. 국내 소비자가 생굴을 섭취할 때 병원성 장염 비브리오균으로 발생하는 위해는 식중독이 주로 발생하는 4월, 10월, 11월에 $5.71{\times}10^{-5}$ (5퍼센타일 $2.71{\times}10^{-8}$, 95퍼센타일 $1.03{\times}10^{-4}$로 추정되었다. 본 연구에서 생굴의 장염비브리오 위해의 영향인자는 소비시점 생굴의 장염비브리오균의 오염수준, 생굴 섭취량, 병원성 인자(tdh or trh 유전자)의 존재 여부, 상온의 노출온도 및 시간으로 나타났으며 위해관리방안을 제시하였다.

• 환경생물
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• 제30권1호
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• pp.71-76
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• 2012

Spills of $M/V$ Hebei Spirit on $7^{th}$ December 2007 caused a seriously damage to the ecosystem of Korean coast. Of these, microbial communities (i.e., attached benthic micro-algae) were reported to be sentive to the environmental change so it can be used for ecological risk assessment. Our experiment was designed to examine the ecological risk assessments for oil pollutant using benthic attached algal community on the artificial substrates of acrylic plates. Field monitoring in the culture system was conducted in Jangmok Bay. The abundances of attached micro-algae on artificial substrates gradually increased with increasing of sampling times. Among them, diatoms were the most important colonizer of coastal water, with the genera $Cylindrotheca$ and $Navicular$ most abundant. In particular, developed the culture system has correctly measured qualitative and quantitative abundance of attached micro-algae because same acrylic plates as artificial substrates were used. Thus, this culture system may be directly applied to the ecological risk experiments of microbial community structure from oil pollutants.

• Fisheries and Aquatic Sciences
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• 제22권11호
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• pp.26.1-26.7
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• 2019

Background: The monitoring of pathogens of fishery auction markets is important to obtain safe fishery products regarding hygiene and sanitation. In this study, aerobic, coliform, Escherichia coli, and Vibrio cholerae were monitored in the fishery products and environmental samples obtained from fishery auction markets. Methods: The fishery products (flounder, octopus, skate, rock cod, sea bass, snail, monkfish, flatfish, comb pen shell, corb shell, conger eel, hairtail, croaker, and pilchard) were placed in filter bags, and the environmental samples (samples from the water tanks at the fishery auction markets, seawater from the fishery distribution vehicles, ice from wooden or plastic boxes, and surface samples from wooden and plastic boxes used for fish storage) were collected. Aerobic bacteria, E. coli, and coliform in the samples were enumerated on aerobic count plates and E. coli/coliform count plates, respectively. For V. cholerae O1 and V. cholerae non-O1 quantification, most probable number (MPN)-PCR analysis was performed. Results: Aerobic and coliform bacteria were detected in most samples, but E. coli was not detected. Wooden boxes were contaminated with high levels of aerobic and coliform bacteria in all seasons (spring, summer, and fall). During fall, V. cholerae non-O1 were detected in snails, hairtails, croakers, flatfishes, pilchards, plastic boxes, and water samples. Conclusions: These results indicate an increased prevalence of V. cholerae contamination in fishery products in fall, including food contact samples, which can be vehicles for cross-contamination.

• 축산식품과학과 산업
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• 제5권1호
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• pp.53-56
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• 2016

• 식품과학과 산업
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• 제46권1호
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• pp.13-25
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• 2013

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2018년도 하계 학술발표초록집
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• pp.131-131
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• 2018

• Journal of Preventive Veterinary Medicine
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• 제42권4호
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• pp.143-147
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• 2018

The present study aimed to develop growth prediction models of Listeria monocytogenes in processed meat products, such as mixed pressed hams, to perform accurate microbial risk assessments. Considering cold storage temperatures and the amount of time in the stages of consumption after opening, the growth of L. monocytogenes was determined as a function of temperature at 0, 5, 10, and $15^{\circ}C$, and time at 0, 1, 3, 6, 8, 10, 15, 20, 25, and 30 days. Based on the results of these measurements, a Baranyi model using the primary model was developed. The input parameters of the Baranyi equation in the variable temperature for polynomial regression as a secondary model were developed: $SGR=0.1715+0.0199T+0.0012T^2$, $LT=5.5730-0.3215T+0.0051T^2$ with $R^2$ values 0.9972 and 0.9772, respectively. The RMSE (Root mean squared error), $B_f$ (bias factor), and $A_f$ (accuracy factor) on the growth prediction model were determined to be 0.30, 0.72, and 1.50 in SGR (specific growth rate), and 0.10, 0.84, and 1.35 in LT (lag time), respectively. Therefore, the model developed in this study can be used to determine microorganism growth in the stages of consumption of mixed pressed hams and has potential in microbial risk assessments (MRAs).

• 한국환경농학회지
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• 제40권4호
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• pp.248-259
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• 2021

BACKGROUND: Contaminated water was a major source of food-borne pathogens in various recent fresh produce-related outbreaks. This study was conducted to investigate the microbial contamination level and correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water by logistic regression analysis. METHODS AND RESULTS: Agricultural water was collected from 457 sites including surface water (n=300 sites) and groundwater (n=157 sites) in South Korea from 2018 to 2020. Sanitary indicator bacteria (total coliform, fecal coliform, and Escherichia coli) and food-borne pathogens (pathogenic E. coli, E. coli O157:H7, Salmonella spp., and Listeria monocytogenes) were analyzed. In surface water, the coliform, fecal coliform, and E. coli were 3.27±0.89 log CFU/100 mL, 1.90±1.19 log CFU/100 mL, and 1.39±1.26 log CFU/100 mL, respectively. For groundwater, three kinds of sanitary indicators ranged in the level from 0.09 - 0.57 log CFU/100 mL. Pathogenic E. coli, Salmonella and Listeria monocytogenes were detected from 3%-site, 1.5%- site, and 0.6%-site water samples, respectively. According to the results of correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens by logistic regression analysis, the probability of pathogen detection increased individually by 1.45 and 1.34 times as each total coliform and E. coli concentration increased by 1 log CFU/100mL. The accuracy of the model was 70.4%, and sensitivity and specificity were 81.5% and 51.7%, respectively. CONCLUSION(S): The results indicate the need to manage the microbial risk of agricultural water to enhance the safety of fresh produce. In addition, logistic regression analysis is useful to analyze the correlation between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water.

• 한국식품조리과학회지
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• 제25권3호
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• pp.297-305
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• 2009

This study aimed to determine the physicochemical, microbiological, and sensory qualityies of steamed chicken samples by prepared with combined additions of oregano-allspice and Ascorbic acid (OA/AA), and processed by a sous-vide cook - chill system. The hurdle effect of the OA/AA addition was examined in terms of microbial stability improvements and their effects on sensory were also evaluated. First, the microbial risk was lowered and chicken quality was good with the addition of the OA/AA hurdle as compared to the control. Second, over various days of storage, the microbial quality of the OA/AA samples was relatively high. In standard plate counts, the control group presented a bacteria level of 2.75 LogCFU/g at 10 days of storage, but the OA/AA groups were had counts of 2.38, 1.89 and 1.81 LogCFU/g, respectively. And at 15 days of storage, the control group had a level of 3.65 LogCFU/g whereas the OA/AA groups had counts of 3.55, 3.54, and 3.24 LogCFU/g, respectively. Lastly, the sensory scores of the OA/AA groups were higher than those of the control group. Accordingly, overall microbial and sensory characteristics were better in the OA/AA hurdle groupsf than in the control group. Thus, the combined addition of Oregano-allspice and Ascorbic acid may be and alternative means for extending shelf - life.