• Title/Summary/Keyword: microbial property

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Control of Enzymatic Degradability of Microbial Polyester by Surface Modification (표면 개질을 통한 미생물합성 폴리에스테르의 효소분해속도 조절)

  • 이원기
    • Journal of Environmental Science International
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    • v.11 no.12
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    • pp.1315-1320
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    • 2002
  • Since the enzymatic degradation of microbial poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] (P(3HB-co-3HV)) initially occurs by a surface erosion process, a degradation behavior could be controlled by the change of surface property. In order to control the rate of enzymatic degradation, plasma gas discharge and blending techniques were used to modify the surface of microbial P(3HB-co-3HV). The surface hydrophobic property of P(3HB-co-3HV) film was introduced by CF$_3$H plasma exposure. Also, the addition of small amount of polystyrene as a non-degradable polymer with lower surface energy to P(3HB-co-3HV) has been studied. The enzymatic degradation was carried out at 37 $^{\circ}C$ in 0.1 M potassium phosphate buffer (pH 7.4) in the presence of an extracellular PHB depolymerase purified from Alcaligenes facalis T1. Both results showed the significant retardation of enzymatic erosion due to the hydrophobicity and the enzyme inactivity of the fluorinated- and PS-enriched surface layers.

Relationship between Chemical Property and Microbial Activity of Reclaimed Tidal Lands at Western Coast Area in Korea

  • Ko, Eun-Seong;Joung, Ji-An;Kim, Chang-Hwan;Lee, Su Hwan;Sa, Tongmin;Choi, Joon-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.4
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    • pp.254-261
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    • 2014
  • The scientific information between microbial activities and chemical properties of reclaimed tidal soil is not enough to apply for reclamation projects. This study was conducted to investigate the relation between chemical properties and microbial activities of reclaimed tidal lands located at western coastal area (25 samples from Nampo, Ewon, Sukmoon and Shihwa sites). Most of the reclaimed soils showed chemical characteristics as salinity soil except Nampo site. The major component influenced the salinity of reclaimed soil was identified as a sodium from the relationship between EC and exchangeable cation. With an increase in EC of soil, the population of mesophilic bacteria decreases whereas halotolerant and halophilic bacteria increases. The population of mesophilic bacteria increased with an increase in both organic matter and dehydrogenase activity. However, the population of halotolerant and halophilic bacteria decreased with an increase in organic matter. Based on the relation between chemical property and microbial activity of reclaimed tidal soil, electrical conductivity and organic matter as chemical properties of soil, population of mesophilic bacteria, halotolerant and halophilic bacteria and dehydrogenase activity as microbial activities could be the major parameters for reclamation process.

Microbial Transglutaminase Improves the Property of Meat Protein and Sausage Texture Manufactured with Low-quality Pork Loins

  • Katayama, K.;Chin, K.B.;Yoshihara, S.;Muguruma, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.1
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    • pp.102-108
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    • 2006
  • Microbial transglutaminase (MTGase) was investigated to determine whether it was an effective binding agent for the processing of low-quality pork loins. MTGase especially promoted the coagulation of myosin heavy chain (MHC). However, the effect of MTGase on MHC from low-quality meat was less than that from the normal meat when the reaction time was not enough. The breaking strength of the heat-induced gel made of myosin B from low-quality meat with MTGase was lower than that of normal meat. Sausage made with low-quality meat with MTGase did not exhibit improved hardness, as compared to that made with normal meat. Results of this study indicated that use of low-quality meat in the manufacture of sausage was feasible to get textural property equal to that of normal meat sausage, when a half or more of the raw material was normal meat and MTGase was used in the sausage.

Surface Modification and Enzymatic Degradation of Microbial Polyesters by Plasma Treatments (플라즈마를 이용한 미생물합성 폴리에스테르의 표면개질과 효소분해성)

  • Kim, Jun;Lee, Won-Ki;Ryou, Jin-Ho;Ha, Chang-Sik
    • Journal of Adhesion and Interface
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    • v.7 no.2
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    • pp.19-25
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    • 2006
  • Since the enzymatic degradation of microbial poly(hydroxylalkanoate)s (PHAs), such as poly[(R)-3-hydroxybutyrate] and poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] initially occurs by a surface erosion process, their degradation behaviors can be controlled by the change of surface property. In order to control the rate of enzymatic degradation, plasma modification technique was applied to change the surface property of microbial PHAs. The surface hydrophobic and hydrophilic properties of PHA films were introduced by $CF_3H$ and $O_2$ plasma exposures, respectively. The enzymatic degradation was carried out at $37^{\circ}C$ in 0.1 M potassium phosphate buffer (pH 7.4) in the presence of an extracellular PHB depolymerase purified from Alcaligenes facalis T1. The results showed that the significant retardation of initial enzymatic erosion of $CF_3H$ plasma-treated PHAs was observed due to the hydrophobicity and the enzyme inactivity of the fluorinated surface layers while the erosion rate of $O_2$ plasma-treated PHAs was not accelerated.

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Effect of Microbial Treatment Methods on Biogrout (미생물 처리 방법이 바이오그라우트에 미치는 영향)

  • Kim, Daehyeon;Park, Kyungho;Kim, Hochul;Lee, Yonghee
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.5
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    • pp.51-57
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    • 2012
  • The purpose of study is to understand the possibility of biogrout of soil induced by bacteria. Microbial Calcium Carbonate Precipitation(MCP) has been analysed using the microorganism Bacillus Pasteurii. In order to understand the biogrout of soft ground treated with microbial calcium carbonate precipitation, four types of specimens(sterilization soil, non-sterilization soil, reaction solution and microorganism solution with pre-treatment mix and reaction solution and microorganism solution with post-treatment mix) were made. Scanning Electron Microscope(SEM), EDX and X-ray diffraction(XRD) analyses were performed on the soft ground specimens. On the basis of the preliminary results, it appears that microbial treatment methods using calcium carbonate precipitation may be possible to improve property of biogrout.

Effect of Microbial Fermentation Compost by Cultivating Area (栽培地域 差異에 따른 微생物 발효퇴비의 施용效果)

  • 이종성;정원복;정순재;오주성;강경희;김회태
    • Korean Journal of Organic Agriculture
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    • v.10 no.1
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    • pp.87-97
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    • 2002
  • This study was conducted to investigate the effect of microbial fermentation compost on the growth, yields and components of Altari Radish, and the soil physicochemistry property by cultivating area. microbial fermentation compost, 90kg/10a, 180kg/10a, 270kg/10a, chemical fertilizer plot were used. The results were summarized as follows ; Change of soil microbial after application microbial fermentation compost, The number of bacteria and actinomycetes in developed land showed the increased tendency. The application effects of microbial fermentation compost on the growth and yield of Altari radish were increased by excessive application with all kinds of microbial fermentation compost, especially, treatment plot at 270kg/10a was the highest. Change of components in Altari Radish was not different between treatments.

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Skin Patch Test and Antibacterial Properties of the Anti-microbial Agent and Melamine Resin Blend Treated Fabric (멜라민 수지와 항균제 혼합 수지 가공 직물의 항균성과 피부 적합성)

  • Chun, Tae-Ill;Park, Jung-Whan
    • Fashion & Textile Research Journal
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    • v.7 no.1
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    • pp.81-84
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    • 2005
  • Skin patch test and antibacterial properties of the reaction products between poly(hexamethyl biguanide) hydrochloride and trimethylol melamine on textile fabrics were examined. Antibacterial activities of anti-microbial agent treated samples are very good. The reduction ratios against four kinds of colonies are 99.9 % after repeated laundering ten times. Skin patch test results for anti-microbial agent treated samples are almost-negative by Hi-scope judgement and macroscopical judgement.

Effectiveness of external agents in polluted sedimentary area

  • Alam, Md. Mahabub;Haque, Md. Niamul;Cho, Daechul;Kwon, Sung-Hyun
    • Environmental Engineering Research
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    • v.21 no.1
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    • pp.52-57
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    • 2016
  • Sediment is a useful natural source but deteriorated continually by anthropogenic and industrial sources. Therefore, it is imperative to search a suitable method for improving or restoring sediment quality. Sediment has been tested to identify the effects of some external agents on a polluted area for 28 days. Chemical analysis and total viable counts (TVC) test have been conducted for 4 days interval to assess their performance. The analyses of chemical oxygen demand (COD), acid volatile sulfide (AVS), total phosphorous (T-P), total nitrogen (T-N) indicate that the chemical agents was more efficient to improve sediment quality whereas the microbial agent was more efficient for nutrient releasing from sediment. Oxygen releasing property of the chemical agent was thought to be providing with more congenial environment for the higher growth of the bacterial community than the direct application of microbial agents.

Bacterial Behavior in Soil under Electric Field and its Effect on Electrokientic Bioremediation (전기장하 토양내에서 미생물 이동특성과 동전기 생물학적복원의 효과)

  • Kim, Sang-Joon;Park, Ji-Yeon;Lee, You-Jin;Yang, Ji-Won
    • KSBB Journal
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    • v.21 no.3
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    • pp.175-180
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    • 2006
  • In this study, it could be found that the microbial movement in soil under electric field mainly occurred by electrophoresis and electroosmosis. The contribution of electrophoresis on the microbial mobility and flux was generally higher than that of electroosmosis. In the electrokinetic(EK) bioremediation of a pentadecane-contaminated soil, the microbial population increased simultaneously at anode and cathode regions of the soil specimen because both electrophoresis and electroosmosis affected on the microbial movement. After initial operation, the microbial population was high in order of anode, middle, and cathode regions due to their negatively-charged surface and oxygen generation at anode. However, the uniform contaminant removal was achieved by the microbial movement with two-directionality.

Effect of Continuous Application of Organic Farming Materials on the Soil Physicochemistry Property and Plant Growth, Yield and Components of Tomato (유기농 자재의 연용이 토양의 이화학적 성질과 토마토의 생육, 수량 및 체내성분에 미치는 영향)

  • 오주성;이종성;강경희;김회태;정원복;정순재
    • Korean Journal of Organic Agriculture
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    • v.9 no.1
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    • pp.75-89
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    • 2001
  • This study the effects of the application of organic farming materials on the soil Physicochemistry property and plant growth, yield and components of Tomato were compared with conventional culture. The results obtained from the experiment are summarized as follows : 1. The effect of soil chemical properties after application organic farming materials, The amounts of pH and O.M. in N, P, K treated plot were few of change, To the contrary, fermentation compost and microorganisms fermentation compost treated plot were some increase, Amount of $P_2$$O_{5}$, Ca and K increase in comparison with the N, P, K treated plot. 2. Changes of soil microbial after application organic farming materials, The number of bacteria, actinomycetes and fungi in N, P, K treated plot were appeared definite direction. to the contrary, The number of bacteria and actimycetes in fermentation compost and microorganisms fermentation compost treated plot showed the increased tendency, The number of fungi showed the decreased tendency. 3. Effect of organic farming materials application on the growth and yield of tomato was superior in order of microorganisms fermentation compost plot〉 chemical fertilizers plot〉 fermentation compost plot. especially, chicken manure + microorganisms fermentation compost treated plot was the highest. 4. Effect of organic farming materials application on the components of tomato were not different.

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