• Title/Summary/Keyword: microbial content

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Dehydrogenase Activity and Physico-chemical Characteristics of Golf Course Soils in Kyonggi Province (경기도 골프장 토양의 탈수소효소 활성과 물리화학적 특성)

  • Lee, In-Sook;Kim, Ok-Kyung
    • The Korean Journal of Ecology
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    • v.17 no.2
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    • pp.143-148
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    • 1994
  • The relationships between microbial activity and physico-KDICical characteristics of soils were investigated in three golf courses of Kwanak, Gold and Korea Country Clubs, with different open years. The soil samples were collected in tee, fairway and rough. There were ranges of 4.80-5.55 in pH, $25.55-98.50{\mu}S$ / cm in conductivity, 10.96-16.73% in moisture content, 0.18-0.36g / g in water holding capacity, 3.68-5.39% in organic matter, and 0.10-0.25% in total nitrogen. Dehydrogenase activity(DHA) as an index of soil microbial activity was determined. DHA values of soil were $69.83-314.43{\mu}$g / g in three courses and showed the order of Kwanak>Gold>Korea Country Club with open year. This indicates that DHA was affected by several fertilizer treatments rather than herbicide and pesticide treatments. DHA was significantly different with golf clubs as well as golf courses and positively correlated with water holding capacity and total nitrogen.

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Development and Application of Natural Antimicrobial Agent Isolated from Grapefruit Seed Extract (Grapefruit 종자추출물로부터 광범위 항균제 개발 및 응용에 관한 연구)

  • 조성환;이상열;김재원;고경혁;서일원
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.33-39
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    • 1995
  • The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed coparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

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Study on Application of Microbial Fertilizers to the Red Pepper (생균제 미생물비료의 고추시비에 관한 연구)

  • 김경제
    • Korean Journal of Organic Agriculture
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    • v.6 no.2
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    • pp.63-74
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    • 1998
  • The result of application of the organic manure obtained from livestocks fed with BLCSs in the red pepper under structure were as follow. The no. of fruit. fruit weight. fruit length. fruit width. and sugar content of red pepper treated with dong of fowl(BLCS) were higher, also. The contents of total N, P2O5, K2O, CaO, MgO, and cap-saicin in the red pepper fruit of treated with Dung of fowl(BLCS) were the highest, and the second was cattle dropping(BLCS). The contents of OM, P2O5, K, Ca, Mg, and CEC of soil treated with microorganism were the highest in the cattle dropping(BLCS) in any other plots, however. In general, effect of cattle manure fed with BLCS on the red pepper yield and microbial activity were higher than cattle manure did not feed BLCS.

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A study for the quality of vegetable dishes without heat treatment in foodservice establishments (단체급식소에서 이용되는 전처리 식품 중 생채소의 품질에 관한 연구)

  • 김혜영;차재맹
    • Korean journal of food and cookery science
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    • v.18 no.3
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    • pp.309-318
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    • 2002
  • The purposes of this study were to estimate the microbial and physicochemical quality of vegetable dishes without heat treatment such as sengchae, a traditional Korean vegetable dish, and to suggest a safer method of preparation. Platycodon sengchae and vegetable salad were monitored from the ingredient to final product before serving while storing at different temperature (4, 10$^{\circ}C$) and period (1, 2, 4, 7 days) at foodservice establishments. The results showed that the storage temperature pH, Aw, moisture content, and microbial loads are the important factors affecting the quality of vegetable dishes without heat treatment, and a thorough hygienic management from the purchase to the preparation of the dishes is needed to secure the quality of prepared foods in the foodservice establishments.

Microbial Diversity Information Facility: Bacteriology Insight Orienting System (BIOS)

  • Shimura, Junko;Shimiz, Hideyukiu;Tsuruwaka, Keiji;Moritani, Yukimitsu;Miyazaki, Kenji;Tsugita, Akira;Watanabe, Makoto M.
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.04a
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    • pp.135-141
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    • 2000
  • Global Biodiversity is common interest of humans for better health and sustainable development of the society. To provide access and analysis on microbial diversity information, Bacteriology Insight Orienting System (BIOS) has been developed. BIOS contains 6402 species and subspecies names of bacteria and archaea, 2606 names of cyanobacteria by March 2000. BIOS of which web based analytical tool provides windows to compare the results of phylogenetic analysis based on 16S rDNA sequence and the results of cluster analysis on proteome profiling. The sequence data and 2 dimensional gel electrophoresis analysis data were accumulated in BIOS database content for cyanobacteria reclassification and taxonomy. (BIOS URL: http.://www-sp2000ao.nies.go.jp/bios/index.html).

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Complete genome sequence of biofilm-producing strain Staphylococcus xylosus S170 (생물막 생성 Staphylococcus xylosus S170 균주의 유전체 분석연구)

  • Hong, Jisoo;Roh, Eunjung
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.167-168
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    • 2018
  • Here we report the complete genome sequence of Staphylococcus xylosus S170, strong biofilm-producing strain, which comprised a single circular 2,910,005 bp chromosome and 32.97% G + C content. The genome included 2,674 protein-coding sequences, 22 rRNA genes, and 57 tRNA genes. Gene analysis of S. xylosus S170 could contribute to better understanding of biofilm-forming mechanisms.

Effect of Irradiated Red Pepper Powder on Kimchi Quality during Fermentation

  • Lee, Seung-Cheol
    • Preventive Nutrition and Food Science
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    • v.9 no.3
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    • pp.218-221
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    • 2004
  • Irradiated red pepper powder (IRPP) was tested for its ability to retard fermentation and to maintain a high quality of Kimchi by the reduction of the initial microbial load. Kimchi containing IRPP at the doses of 0, 5, 10, 15, or 20 kGy was prepared. Quality indices for Kimchi in this study were pH, titratable acidity, reducing sugar content, total microbial count, lactic acid bacterial load, and sensory evaluation. Based on the pH and titratable acidity, the Kimchi with IRPP showed a retarded fermentation until 15 days. The number of the total aerobes and lactic acid bacteria of the Kimchi with IRPP were lower by about 1 log CFU/mL compared to control at day 0, however, the counts increased to 8.5 log CFU/mL after 10 days, which was similar to the control group. Kimchi that was fermented with 5 kGy IRPP was better than control and other treatments in odor and color, whereas the control scored highest in taste. Addition of IRPP showed a limited retardation of Kimchi fermentation without other quality deterioration.

Evaluation of Optimum Moisture Content for Composting of Beef Manure and Bedding Material Mixtures Using Oxygen Uptake Measurement

  • Kim, Eunjong;Lee, Dong-Hyun;Won, Seunggun;Ahn, Heekwon
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.5
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    • pp.753-758
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    • 2016
  • Moisture content influences physiological characteristics of microbes and physical structure of solid matrices during composting of animal manure. If moisture content is maintained at a proper level, aerobic microorganisms show more active oxygen consumption during composting due to increased microbial activity. In this study, optimum moisture levels for composting of two bedding materials (sawdust, rice hull) and two different mixtures of bedding and beef manure (BS, Beef cattle manure+sawdust; BR, Beef cattle manure+rice hull) were determined based on oxygen uptake rate measured by a pressure sensor method. A broad range of oxygen uptake rates (0.3 to 33.3 mg $O_2/g$ VS d) were monitored as a function of moisture level and composting feedstock type. The maximum oxygen consumption of each material was observed near the saturated condition, which ranged from 75% to 98% of water holding capacity. The optimum moisture content of BS and BR were 70% and 57% on a wet basis, respectively. Although BS's optimum moisture content was near saturated state, its free air space kept a favorable level (above 30%) for aerobic composting due to the sawdust's coarse particle size and bulking effect.

지중오존산화시 토양유기물질과 수분이 토착미생물의 생존과 재성장에 미치는 영향

  • 손규동;정해룡;최희철;김수곤;양지원
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.334-337
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    • 2003
  • This study was carried out to investigate the effect of soil properties, such as soil organic matter(SOM) content and water content on die-off and regrowth of indigenous microbes due to in-situ ozonation. Four different soils were collected and the soil samples applied to different ozonation time(0-360 min) were incubated during 4 weeks. Population of the indigenous microbes was monitored during incubation period. The number of indigenous microbes in all samples dramatically decreased (more than 90%) within 30 minutes of ozone injection. With increased ozonation time by 360 minutes, the number of the indigenous microbes decreased by 99.99% in all samples. Die-off of the indigenous microbes due to ozone treatment was inversely proportional to SOM and water content. Especially, sample 3 and Sample 4 containing relatively high SOM content and water content showed high regrowth rate, and this resulted from the increase of water soluble and biodegradable organic fraction in soil water after ozone treatment. Soil sample ozonated for 360 minutes showed minor increase in microbial population during 4 weeks of incubation period.

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Implementation of an Efficient Microbial Medical Image Retrieval System Applying Knowledge Databases (지식 데이타베이스를 적용한 효율적인 세균 의료영상 검색 시스템의 구현)

  • Shin Yong Won;Koo Bong Oh
    • Journal of the Korea Society of Computer and Information
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    • v.10 no.1 s.33
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    • pp.93-100
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    • 2005
  • This study is to desist and implement an efficient microbial medical image retrieval system based on knowledge and content of them which can make use of more accurate decision on colony as doll as efficient education for new techicians. For this. re first address overall inference to set up flexible search path using rule-base in order U redure time required original microbial identification by searching the fastest path of microbial identification phase based on heuristics knowledge. Next, we propose a color ffature gfraction mtU, which is able to extract color feature vectors of visual contents from a inn microbial image based on especially bacteria image using HSV color model. In addition, for better retrieval performance based on large microbial databases, we present an integrated indexing technique that combines with B+-tree for indexing simple attributes, inverted file structure for text medical keywords list, and scan-based filtering method for high dimensional color feature vectors. Finally. the implemented system shows the possibility to manage and retrieve the complex microbial images using knowledge and visual contents itself effectively. We expect to decrease rapidly Loaming time for elementary technicians by tell organizing knowledge of clinical fields through proposed system.

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