• Current Optics and Photonics
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• v.6 no.6
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• pp.550-564
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• 2022

Optical microscopy is a useful tool for study in the biological sciences. With an optical microscope, we can observe the micro world of life such as tissues, cells, and proteins. A fluorescent dye or a fluorescent protein provides an opportunity to mark a specific target in the crowd of biological samples, so that an image of a specific target can be observed by an optical microscope. The optical microscope, however, is constrained in resolution due to diffraction limit. Super-resolution microscopy made a breakthrough with this diffraction limit. Using a super-resolution microscope, many biomolecules are observed beyond the diffraction limit in cells. In the case of volumetric imaging, the super-resolution techniques are only applied to a limited area due to long imaging time, multiple scattering of photons, and sample-induced aberration in deep tissue. In this article, we review recent advances in super-resolution microscopy for volumetric imaging. The super-resolution techniques have been integrated with various modalities, such as a line-scan confocal microscope, a spinning disk confocal microscope, a light sheet microscope, and point spread function engineering. Super-resolution microscopy combined with adaptive optics by compensating for wave distortions is a promising method for deep tissue imaging and biomedical applications.

• 한국지구물리탐사학회:학술대회논문집
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• 2004.06a
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• pp.3-17
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• 2004

Space-borne Earth observation technique is one of the most cost effective and rapidly advancing Earth science research tools today and the potential field and micro-wave radar applications have been leading the discipline. The traditional optical imaging systems including the well known Landsat, NOAA - AVHRR, SPOT, and IKONOS have steadily improved spatial imaging resolution but increasing cloud covers have the major deterrent. The new Earth observation satellites ENVISAT (launched on March 1 2002, specifically for Earth environment observation), ALOS (planned for launching in 2004 - 2005 period and ALOS stands for Advanced Land Observation Satellite), and RADARSAT-II (planned for launching in 2005) all have synthetic aperture radar (SAR) onboard, which all have partial or fully polarimetric imaging capabilities. These new types of polarimetric imaging radars with repeat orbit interferometric capabilities are opening up completely new possibilities in Earth system science research, in addition to the radar altimeter and scatterometer. The main advantage of a SAR system is the all weather imaging capability without Sun light and the newly developed interferometric capabilities, utilizing the phase information in SAR data further extends the observation capabilities of directional surface covers and neotectonic surface displacements. In addition, if one can utilize the newly available multiple frequency polarimetric information, the new generation of space-borne SAR systems is the future research tool for Earth observation and global environmental change monitoring. The potential field strength decreases as a function of the inverse square of the distance between the source and the observation point and geophysicists have traditionally been reluctant to make the potential field observation from any space-borne platforms. However, there have recently been a number of potential field missions such as ASTRID-2, Orsted, CHAMP, GRACE, GOCE. Of course these satellite sensors are most effective for low spatial resolution applications. For similar objects, AMPERE and NPOESS are being planned by the United States and France. The Earth science disciplines which utilize space-borne platforms most are the astronomy and atmospheric science. However in this talk we will focus our discussion on the solid Earth and physical oceanographic applications. The geodynamic applications actively being investigated from various space-borne platforms geological mapping, earthquake and volcano .elated tectonic deformation, generation of p.ecise digital elevation model (DEM), development of multi-temporal differential cross-track SAR interferometry, sea surface wind measurement, tidal flat geomorphology, sea surface wave dynamics, internal waves and high latitude cryogenics including sea ice problems.