• Title/Summary/Keyword: miR-29a

Search Result 226, Processing Time 0.031 seconds

Objective and Subjective Quality Characteristics of Pork Longissimus Muscle as a Function of the Ultimate pH

  • Cho, Byung-Wook;Oliveros, Maria Cynthia;Park, Kyoung-Mi;Do, Kyoung-Tag;Lee, Ki-Hwan;Seo, Kang-Seok;Choi, Jae-Gwan;Lee, Moon-Jun;Cho, In-Kyung;Choi, Byoung-Chul;Ryu, Kyeong-Seon;Hwang, In-Ho
    • Food Science of Animal Resources
    • /
    • v.29 no.6
    • /
    • pp.685-694
    • /
    • 2009
  • The aim of the present study was to evaluate the variation in ultimate pH of commercial populations of pure-breed (Landrace, Duroc and Yorkshire) pig's longissimus muscles and their effect on objective meat quality traits and sensory characteristics. Fifty boars were sampled from 184 pigs, which were reared at three breeding farms and slaughtered at a commercial abattoir. The selection was determined based on ultimate pH, and animals were segregated into three groups: low pH (pH $\leq$ 5.5, n=13), medium pH (pH 5.5 to 5.6, n=18) and high pH (pH $\geq$ 5.6, n=16). The breeds had no significant effects; however, pigs with a higher ultimate pH had significantly (p<0.05) higher intramuscular fat content, lower level of polyunsaturated fatty acids, lower level of lipid oxidation and higher eating quality compared to those with lower ultimate pH. As the ultimate pH increased, the relative proportion of C14:0, C16:0 and C18:1 increased while C18:2n6 and C20:4n6 decreased. The present study demonstrates that the economic value of pigs can be characterized by the ultimate pH and/or intramuscular fat content. However, these results do not necessarily indicate that a high ultimate pH directly corresponds to high intramuscular fat content and vice versa.

Detection and Differentiation of Intentional and Unintentional Mixture in Raw Meats Using Real-time PCR (Real-time PCR을 이용한 식육원료의 의도적, 비의도적 혼입 판별법 개발)

  • Kim, Kyu-Heon;Kim, Mi-Ra;Park, Young-Eun;Kim, Yong-Sang;Lee, Ho-Yeon;Park, Yong-Chjun;Kim, Sang Yub;Choi, Jang Duck;Jang, Young-Mi
    • Journal of Food Hygiene and Safety
    • /
    • v.29 no.4
    • /
    • pp.340-346
    • /
    • 2014
  • In this study, the detection method was developed using real-time PCR to distinguish 4 species (bovine, porcine, horse, and chicken) of raw meats. The genes for distinction of species about meats targeted at 12S rRNA and 16S rRNA parts in mitochondrial DNA. Probes were designed to have a 5' FAM and a TAMRA at the 3' end. This study is to develop 4 species-specific primer and probes about raw materials and real-time PCR on 10 strains to observe the products of non-specific signal for similar species. As a result, any non-specific signal were not detected among each other. Real-time PCR method was developed for quantitation and identification of intentional and unintentional mixture in ground mixed meat (The difference of $C_T$ value between intentional mixture and 100% meat: $${\leq_-}$$ cycles, The difference of $C_T$ value between unintentional mixture and 100% meat: $${\geq_-}$$ cycles). The detection and differentiation of intentional and unintentional mixture in this study would be applied to food safety management for eradication of adulterated food distribution and protection of consumer's right.

Effect of Myers-Briggs Type Indicator(MBTI) Personality Type, Emotional Intelligence, Sress Coping Strategies on Nursing student's Adjustment to College Life (간호대학생의 MBTI성격유형, 감성지능, 스트레스 대처방식이 대학생활적응에 미치는 영향)

  • Han, Young-Mi;Kim, Sun-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.18 no.8
    • /
    • pp.124-134
    • /
    • 2017
  • The purpose of this study was to investigate the MBTI personality type, emotional intelligence, and stress coping strategies of nursing students and to examine the influence of these factors on their level of college adjustment.The subjects were 267 nursing students who are located in Chonnam city. The survey was conducted from March 6th to March 31st, 2017. The level of college adjustment showed a close correlation with the emotional intelligence(r=.29, p<.001) and stress coping strategies(r=.27, p<.001), and the level of MBTI personality type was not statistically insignificant in the nursing students. It was found that the effect of the emotional intelligence and stress coping strategies on the college life adjustment was 17.5%(F=8.03, p<.001). According to the results of this study, it is necessary to develop a systematic management program to integrate the educational curriculum and extra-curricular activities(in order to enhance the) emotional intelligence and stress coping strategies in nursing students. In addition, the type of psychological function and psychological temperament of the MBTI personality type were found not to have influenced the level of college adaptation of the nursing students and this should be confirmedin a large scale sample.

Evaluation of Indoor Mold Exposure Level in dwelling Using DNA-Based Mold Assessment Method (DNA 기반 곰팡이 평가기법을 활용한 주택의 실내 곰팡이 노출수준 평가)

  • Hwang, Eun-Seol;Seo, Sung Chul;Lee, Ju-Yeong;Ryu, Jung-min;Kwon, Myung-Hee;Chung, Hyen-Mi;Cho, Yong-Min;Lee, Jung-Sub
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.28 no.4
    • /
    • pp.382-392
    • /
    • 2018
  • Objective: Allergic diseases such as asthma due to fungal exposure in houses have increased, and proper management is urgent. Mold can grow in the air, floor, walls, and other areas according to environmental conditions, and there are many limitations to the conventional methodology for examining fungal exposure. For this reason, the degree of fungal contamination is being evaluated by ERMI (Environmental Relative Moldiness Index), a quantitative analysis method proposed by the EPA. In this study, we compared ERMI values between water-damaged dwellings and non-damaged ones to evaluate the effectiveness of Korean ERMI values. We also explored the association of ERMI values with the level of airborne mold and characteristics of dwellings. Methods: Floor dust was collected after installing a Dustream collector on the suction port of a vacuum cleaner. The collected samples were filtered to remove only 5 mg of dust, and DNA was extracted using the FastDNA SPIN KIT protocol. Results: The ERMI values were found to be 19.6 (-6.9-58.8) for flooded houses, 7.5 (-29.2-48.3) for leaks/condensation, and 0.8 (-29.2-37.9) for non-damaged dwellings. The airborne concentration of mold for flooded, leakage or condensed, and non-damaged houses were $684CFU/m^3$, $566CFU/m^3$, and $378CFU/m^3$, respectively. The correlation between ERMI values and the levels of airborne mold was low (R = 0.038), but a weakly significant association of the ERMI values with the concentration of particulate matter ($PM_{10}$) was observed as well(R=0.231,P<0.05). Conclusions: Our findings show that the reference value using ERMI can be used to distinguish water-damaged and non-damaged dwellings. It is believed that ERMI values could be a promising tool for assessing long-term fungal exposure in dwellings.

Inhibition of Side Reactions Forming Dimers of Diols in the Selective Hydrogenation of Methacryl Aldehyde (메타아크릴 알데히드의 선택적 수소화에서 2가 알코올의 이합체 형성 부반응 억제효과)

  • Kook-Seung Shin;Mi-Sun Cha;Kyoung-Ku Kang;Chang-Soo Lee
    • Clean Technology
    • /
    • v.29 no.2
    • /
    • pp.79-86
    • /
    • 2023
  • The homogeneous catalyst, Ru-MACHO-BH, selectively performs hydrogenation reactions only on the carbonyl group of α, β-unsaturated aldehyde compounds with extremely high reactivity and selectivity. However, the hydrogenation of α, β-unsaturated aldehydes involves a heterogeneous Diels-Alder reaction, resulting in the formation of significant amounts of byproducts, such as dimers. In this study, we used the Ru-MACHO-BH catalyst (Carbonyl hydrido (tetrahydroborato) [bis (2-diphenyl phosphino ethyl) amino] ruthenium(II)) to selectively hydrogenate the carbonyl group of a specific type of α, β-unsaturated aldehyde called methacryl aldehyde, leading to the synthesis of methallyl alcohol. Simultaneously, we applied diols to inhibit the formation of byproducts. The results demonstrate that monoethylene glycol can significantly reduce the formation of diols. Based on these results, we effectively suppressed the formation of dimers containing vinyl groups in methacryl aldehyde by using hydroquinone, which can efficiently inhibit the chemical interaction of vinyl groups. Consequently, the conversion rate of methacryl aldehyde was increased. Ultimately, by reducing the amount of the expensive homogeneous catalyst Ru-MACHO-BH to 1/10, we achieved a selectivity of over 90% and a yield of over 80% for the desired product, methallyl alcohol. These results provide a method to minimize yield reduction while reducing the usage of expensive catalysts, thereby improving cost-effectiveness. We expect that the reaction could be applied to various kinds of selective hydrogenation and has been successfully run on an industrial scale.

Effect of Temperature on Development and Reproduction of the Emma Field Cricket, Teleogryllus emma(Orthoptera: Gryllidae)

  • Kim, Nam-Jung;Hong, Seong-Jin;Seol, Kwang-Youl;Kim, Seong-Hyun;Ahn, Nan-Hee;Kim, Mi-Ae
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.15 no.1
    • /
    • pp.69-73
    • /
    • 2007
  • To establish the indoor-rearing system of the emma field cricket, Teleogryllus emma, the effects of temperature on development and reproduction of the emma cricket have been studied. The influence of temperature on developmental periods of emma field cricket was investigated under the eight temperatures of 15, 18, 21, 25, 27, 29, 31 and $35^{\circ}C$, under $60{\pm}5%$ R.H. and 16L:8D photoperiod. The developmental periods of T. emma nymphs had a range of 124.8 days to 44.4 days at the temperature of $21^{\circ}C$ and $35^{\circ}C$, respectively. At $15^{\circ}C$ and $18^{\circ}C$, however, all tested individuals died before emergence. The highest survival rate was 90% at $25^{\circ}C$, but there were no statistically significant differences among the temperatures. The adult weight increased with increasing temperatures although the weight at $35^{\circ}C$ was decreased. In addition, the influence of temperature on reproduction of emma field cricket was investigated under three temperatures $22^{\circ}C,\;25^{\circ}C\;and\;28^{\circ}C$, under $60{\pm}5%$ R.H. and 16L:8D photoperiod. The longevity of female/male adults were 65.8/79.2 days, 68.5/67.8 days, 46.8/57.4 days at the temperature $22^{\circ}C,\;25^{\circ}C\;and\;28^{\circ}C$, respectively. The preoviposition periods were 32.5 days at $22^{\circ}C,\;22.9^{\circ}C$ days at $25^{\circ}C$ and 22.1 days at $28^{\circ}C$. The highest average fecundity per female was 737.3 at $25^{\circ}C$.

Allometric Relations of Take-off Speed and Power with Body Mass of Anuran Amphibians

  • Choi, In-Ho;Shin, Jae-Seung;Kim, Mi-Hyun
    • Animal cells and systems
    • /
    • v.2 no.4
    • /
    • pp.477-481
    • /
    • 1998
  • Previous studies have postulated that isometric animals exert similar locomotory capacity (speed, distance) because the amount of energy available for the motion would be the same regardless of body mass (m). To test propriety of this theory, we examined body shape and take-off potential of two frog species, Rana nigromaculata (powerful jumpers) and Bombina orientalis (slow hoppers). Morphological measurements included thigh muscle mass (indicative of total muscle force), hindlimb length (L, determining acceleration distance), and interilial width (shaping take-off motion). To gauge locomotory capacity, take-off speed (v) and take-off angle ($\theta$) were measured from video analyses, and jump distance (R) and take-off Power ($P_{t}$ ) were calculated from equations $R=V^{2}sin2\theta/g$ and ($P_{t}$$㎷^{3}/2L$(where g is the gravitational constant). Scaling exponents of morphometric variables for both species were 0.96-1.11 for thigh muscle mass, 0.28-0.29 for hindlimb length, and 0.30-0.36 for interilial width. Scaling exponents of locomotory performance for the two species were -0.01-0.14 for take-off speed, 0.24-0.31 for jump distance, and 0.66-0.84 for take-off power. The results demonstrate that the frogs of this study showed isometric body shape within species, but that take-off response changed allometrically with body mass, indicating that these data did not fully support the previous proposition. An exception was found in take-off speed of B. orientalis, in which the speed changed little with body mass (slope=-0.01). These findings suggest that the energy availability approach did not properly explain the apparent allometric relations of the take-off response in these animals and that an alternative model such as a power production approach may be worth addressing.

  • PDF

Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

  • Park, Jun Seok;Guevarra, Robin B.;Kim, Bo-Ra;Lee, Jun Hyung;Lee, Sun Hee;Cho, Jae Hyoung;Kim, Hyeri;Cho, Jin Ho;Song, Minho;Lee, Ju-Hoon;Isaacson, Richard E.;Song, Kun Ho;Kim, Hyeun Bum
    • Journal of Microbiology and Biotechnology
    • /
    • v.29 no.9
    • /
    • pp.1391-1400
    • /
    • 2019
  • Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.

Application of ultra-high-temperature ceramics to oxidation-resistant and anti-ablation coatings for carbon-carbon composite (탄소-탄소 복합재의 내삭마 내산화 코팅을 위한 초고온 세라믹스의 적용)

  • Kim, Hyun-Mi;Choi, Sung-Churl;Cho, Nam Choon;Lee, Hyung Ik;Choi, Kyoon
    • Journal of the Korean Crystal Growth and Crystal Technology
    • /
    • v.29 no.6
    • /
    • pp.283-293
    • /
    • 2019
  • As applications in extreme environments such as aerospace, high-energy plasma and radio-active circumstances increases, the demand for materials that require higher melting points, higher mechanical strength and improved thermal conductivity continues to increase. Accordingly, in order to improve the oxidation/abrasion resistance of the carbon-carbon composite, which is a typical heat-resistant material, a method of using ultra high temperature ceramics was reviewed. The advantages and disadvantages of CVD coating, pack cementation and thermal plasma spraying, the simplest methods for synthesizing ultra-high temperature ceramics, were compared. As a method for applying the CVD coating method to C/C composites with complex shapes, the possibility of using thermodynamic calculation and CFD simulation was proposed. In addition, as a result of comparing the oxidation resistance of the TaC/SiC bi-layer coating and TaC/SiC multilayer coating produced by this method, the more excellent oxidation resistance of the multilayer coating on C/C was confirmed.

Comparison of Life Table and Feeding Behavior of Resistance and Susceptible Population of the Green Peach Aphid, Myzus persicae (Hemiptera: Aphididae) against Etofenprox (Etofenprox에 대한 복숭아혹진딧물(Myzus persicae)의 저항성과 감수성 개체군의 생명표와 섭식행동 비교)

  • Jo, Chang-Wook;Park, Cho-Rong;Yoon, Kyu-Sik;Kang, Min-A;Kwon, Hae-Ri;Kang, Eun-Jin;Seo, Mi-Ja;Yu, Yong-Man;Youn, Young-Nam
    • Korean journal of applied entomology
    • /
    • v.48 no.3
    • /
    • pp.301-310
    • /
    • 2009
  • For the comparing of mortality of the resistance and susceptible population of Myzus persicae, etofenprox was treated in the recommended concentration of 200ppm. Mortalities of resistance population were 16.7 and 36.7%, and susceptible population were 86.7 and 86.7% after 24 and 48 hours treatment, respectively. For the detect of cross resistance to other pyrethroids, 6 pyrethroids were examined. Mortalities of susceptible and resistance populations were 90 and 31% to deltamethrin, 92 and 23% to lambda cyhalothrin, 81 and 14% to cypermethrin, 70 and 20% to $\alpha$-cypermethrin, 29 and 28% to fenpropathrin, 84 and 29% to fenvalerate, respectively. It was showed that resistance populations were generally resistive to other pyrethroids. On the other hands, for recognized ecological characteristic of M. persicae susceptible and resistance populations life table was tested on the pepper leaves in the petri dish and on the plant in the pot. This results were showed that intrinsic rate of increase ($r_m$), net reproduction number ($R_0$) and generation time in day ($T_c$) were significantly different between two population in both tested. However, life span and reproduction period were slightly different between them. Otherwise, feeding behaviors were tested using EPG technique with non- and treated etofenprox. First potential drop time of susceptible and resistance population was 73.5 and 257.9 sec with non-treated and 93.3 and 1076.2 sec after treated, respectively. Electrical probing signals were 8.2 and 48.8 times with susceptible and resistance individuals after treated etofenprox, respectively. It was supposed that the resistance is more probings than susceptible population. After treated, total feeding time have more 6,728.9 sec on resistance than 965.5 sec on susceptible population. So, total non penetration time of susceptible population was 3,000 sec longer than resistance population.