• Title/Summary/Keyword: miR-29a

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Anti-Aging Activity of Lavandula angustifolia Extract Fermented with Pediococcus pentosaceus DK1 Isolated from Diospyros kaki Fruit in UVB-Irradiated Human Skin Fibroblasts and Analysis of Principal Components

  • Ha, Ji Hoon;Kim, A Rang;Lee, Keon-Soo;Xuan, Song Hua;Kang, Hee Cheol;Lee, Dong Hwan;Cha, Mi Yeon;Kim, Hye Jin;An, Mi;Park, Soo Nam
    • Journal of Microbiology and Biotechnology
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    • v.29 no.1
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    • pp.21-29
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    • 2019
  • The effects of Lavandula angustifolia extract fermented with Pediococcus pentosaceus DK1 on UVB-mediated MMP-1 expression and collagen decrease in human skin fibroblasts were determined, and the conversion of its components was also analyzed. Fermentation was performed at varying L. angustifolia extract and MRS medium concentrations, and optimal fermentation conditions were selected. L. angustifolia extracts showed decreased cytotoxicity after fermentation in the fibroblasts. UVB-irradiated fibroblasts treated with fermented L. angustifolia extract showed MMP-1 expression 8.2-14.0% lower than that in UVB-irradiated fibroblasts treated with non-fermented extract. This was observed even at fermented extract concentrations lower than those of non-fermented extracts. Fibroblasts treated with fermented L. angustifolia extract showed 20% less reduction in collagen production upon UVB irradiation than those treated with non-fermented extracts. UVB-irradiated fibroblasts treated with fermented L. angustifolia extracts showed 50% higher inhibition of ROS generation than those treated with non-fermented extract. Luteolin and apigenin glycosides of L. angustifolia were converted during fermentation, and identified using RP-HPLC and LC/ESI-MS. Therefore, the effects of L. angustifolia extract on MMP-1 expression and collagen decrease in UVB-irradiated human skin fibroblasts were increased through fermentation by P. pentosaceus.

Hardiness as a Stress-resistance Resource and as a Determinant of Health -promoting Behavior: in a Sample of Nursing Students (강인성이 건강증진행위, 신체증상에 미치는 효과-여대생을 대상으로-)

  • Lee, Mi-Ra;So, Hee-Young;Song, Young-Sin
    • Research in Community and Public Health Nursing
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    • v.9 no.1
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    • pp.21-29
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    • 1998
  • This study was undertaken in order to examine the relationship of hardiness and health-promoting behavior and the effects of hardiness on stress-related physical symptoms. The subjects were 104 female nursing students of one college in Kongju. The instruments used for this study were a survey of general characteristics, hardiness(25 items), health-promoting behavior(44 items), and physical symptoms(35 items), Analysis of data was done by use of mean, Pearson correlation coefficient, stepwise regression and a hierarchical multiple regression with an SAS program. The results of this study are as follows. 1) Significant correlations between health -promoting behavior and subscales of hardiness, that is, control(r=-.35, P<.00l), commitment(r= -.29, P<.0l), and challenge(r= -.23, p<.05) were found. 2) Control was the highest factor predicting health -promoting behavior. 3) Main and buffering effects of hardiness on current physical symptoms were not found.

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Report of 29 unrecorded bacterial species from the phylum Proteobacteria

  • Nam, Yoon-Jong;Beak, Kiwoon;Han, Ji-Hye;Park, Sanghwa;Lee, Mi-Hwa
    • Journal of Species Research
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    • v.7 no.1
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    • pp.60-72
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    • 2018
  • Our study aimed to discover indigenous prokaryotic species in Korea. A total of 29 bacterial species in the phylum Proteobacteria were isolated from freshwater and sediment of rivers and brackish zones in Korea. From the high 16S rRNA gene sequence similarity (${\geq}98.8%$) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to an independent and predefined bacterial species. To our knowledge, there is no official report or publication that has previously described these 29 species in Korea. Specifically, we identified 10, 12, and seven species of eight, 12, and seven genera that belong to classes Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria, respectively; all are reported as previously unrecorded bacterial species in Korea. The Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs for each are also described.

Nitric Oxide and Hydrogen Peroxide Production are Involved in Systemic Drought Tolerance Induced by 2R,3R-Butanediol in Arabidopsis thaliana

  • Cho, Song-Mi;Kim, Yong Hwan;Anderson, Anne J.;Kim, Young Cheol
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.427-434
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    • 2013
  • 2R,3R-Butanediol, a volatile compound produced by certain rhizobacteria, is involved in induced drought tolerance in Arabidopsis thaliana through mechanisms involving stomatal closure. In this study, we examined the involvement of nitric oxide and hydrogen peroxide in induced drought tolerance, because these are signaling agents in drought stress responses mediated by abscisic acid (ABA). Fluorescence-based assays showed that systemic nitric oxide and hydrogen peroxide production was induced by 2R,3R-butanediol and correlated with expression of genes encoding nitrate reductase and nitric oxide synthase. Co-treatment of 2R,3R-butanediol with an inhibitor of nitrate reductase or an inhibitor of nitric oxide synthase lowered nitric oxide production and lessened induced drought tolerance. Increases in hydrogen peroxide were negated by co-treatment of 2R,3R-butanediol with inhibitors of NADPH oxidase, or peroxidase. These findings support the volatile 2R,3R-butanediol synthesized by certain rhizobacteria is an active player in induction of drought tolerance through mechanisms involving nitric oxide and hydrogen peroxide production.

Predictors of Caregivers' First Aid Confidence (요양보호사의 응급처치 수행자신감 예측요인)

  • Soon-Ok Kim;Mi-Hee Kim
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.4
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    • pp.811-824
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    • 2023
  • The purpose of this study was to investigate the communication confidence, self-efficacy, and self confidence in first aid and to identify the predictors of self confidence in first aid. Subjects were 202 caregivers and data were collected by questionnaires from march 1 to 31, 2023. Data were analyzed using t-test, ANOVA, Scheffe's test, Pearson correlation coefficients and Multiple regression analysis using the SPSS 29.0 program. Self-efficacy was a positive correlation with communication confidence (r=.54, p<.001), and self confidence in first aid was a negative correlation with communication confidence(r=-.18, p<.05) and self-efficacy(r=-.31, p<.001). Predictive factors for self confidence in first aid were absence of nurse's aide(β=-.18, p=.009) and self-efficacy(β=-.30, p<.001), and explanatory power was 11.0%(Adj R2=.110, p<.001). Based on the results of this study, to develop and apply an educational program focusing on emergency problems.

Pruritus in Burn Survivors (화상환자의 소양증 특성 조사)

  • Kim, Kyung Ja;Won, Mi Hwa
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.1
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    • pp.45-53
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    • 2016
  • This study examined pruritus to provide the data based on burn patient pruritus. A self-report questionnaire survey was conducted among ninety five burn survivors, who were over 18 years of age with admission periods longer than two weeks. The results indicated that the average burn range was $18.95{\pm}17.10%$, severity was $48.83{\pm}23.96$, and distress was $46.42{\pm}25.80$. A significant difference in pruritus consequences was observed according to frequency. The patients consistently experienced pruritus, even if it happened less often, difficulty in falling asleep (F=4.817, p=.004), poor mood (F= 5.955, p=.015)), and loss of concentration (F=6.483, p<.000). A significant difference in the pruritus consequences according to duration was observed, lasting for 30 minutes for all groups, more than the population and less than 30 minutes, difficulty in falling asleep (F=7.452, p<.000), poor mood (F=3.655, p<.015), and loss of concentration (F=12.564, p<.000). The frequency, duration (r=.29, p=.004), severity (r=.45, p<.000), distress (r=.44, p<.000), and consequences of pruritus in burn patients (r=.29, p=.004) showed a positive correlation. The results of this study could be used as a base material for the care of burn patients experiencing pruritus.

Factors Influencing Preventive Behaviors for COVID-19 in Undergraduate Nursing Students: A Cross-Sectional Descriptive Study

  • Choi, Jeong Sil;Kim, Kyung Mi
    • Journal of Korean Academy of Nursing
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    • v.52 no.6
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    • pp.554-563
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    • 2022
  • Purpose: This study aimed to determine how undergraduate nursing students' knowledge and health beliefs affected their COVID-19-related infection-prevention behaviors. Methods: This study used a descriptive survey. A total of 188 undergraduate nursing students from two universities in South Korea participated in this study. The data were collected from June 2020 to August 2020. Factors influencing infection-prevention behaviors were identified using multiple regression analysis. Results: The participants' mean knowledge level regarding COVID-19 was 84.05 ± 11.78 out of 100. The average health belief score was 2.80 ± 0.32 points out of 5. COVID-19-related preventive health behaviors were correlated with experiences of searching for COVID-19 information (r = .22, p < .01), perceived severity (r = .24, p < .01), perceived benefits (r = .29, p < .01), cues to action (r = .30, p < .01), knowledge (r = .27, p < .01), and perceived barriers (r = - .19, p < .05). Factors that significantly affected COVID-19-related preventive health behaviors were the participants' years of study, experiences regarding COVID-19 prevention education, perceived severity, perceived barriers, and cues to action. Conclusion: COVID-19-related preventive health behaviors are promoted by increasing awareness about the disease and promoting COVID-19 education in nursing curriculums.

Rapid Identification of Diaporthe citri by Gene Sequence Analysis

  • Zar Zar Soe;Yong Ho Shin;Hyun Su Kang;Mi Jin Kim;Yong Chull Jeun
    • Research in Plant Disease
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    • v.29 no.2
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    • pp.130-136
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    • 2023
  • Citrus melanoses caused by Diaporthe citri, has been one of the serious diseases in many citrus orchards of Jeju Island. To protect melanose in citrus farms, a fast and exact diagnosis method is necessary. In this study, diseased leaves and dieback twigs were collected from a total of 49 farms within March to April in 2022. A total of 465 fungal isolates were obtained from a total of 358 isolated plant samples. Among these fungal isolates, 40 representatives of D. citri isolates which were isolated from 22 twigs and 18 leaves on 23 farms were found based on cultural characteristics on potato dextrose agar and conidial morphology. Additionally, the molecular assay was carried out and compared with those by morphological diagnosis. All isolates were identified as D. citri by analyzing the sequences at the internal transcribed spacer (ITS) rDNA region using primers of ITS1/ITS4 or at β-tubulin using primer Btdcitri-F/R. Therefore, based on the present study, where the results of morphological identification of conidial type were consistent with DNA sequence analysis of certain gene, choosing a suitable method for a fast diagnosis of citrus melanose was suggested.

The Effects of Drinking Patterns, Job Stress, and Leisure Satisfaction on Health Promotion Behaviors of Industrial Workers (일 지역사회 산업장 근로자의 음주형태, 직무스트레스 및 여가만족도가 건강증진행위에 미치는 영향)

  • Chang, Koung-Oh;Park, Mi-Hwa
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.3
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    • pp.499-510
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    • 2021
  • This is a descriptive research study on industrial workers to understand the effects of drinking patterns, job stress, and leisure satisfaction on their health promotion behavior. Subjects of the survey were 207 workers undergoing health checkups at three hospitals in U metropolitan city. Data was collected from November 1, 2019, to November 30, 2019, through a structured questionnaire. The IBM SPSS 24.0 program was used to analyze data. The subjects' drinking frequency was 2 to 3 times a week, (36.3%), and the motive was a social gathering (54.6%). The averages were 62.46±5.59 for job stress, 30.29±6.39 leisure satisfaction, 125.64±8.77 for health promotion behaviors, which was higher than the median. Health promotion behavior was found to have a statistically significant positive correlation with job stress (r=.227, p<.001) and leisure satisfaction (r=.542, p<.001). A multiple regression analysis of the factors affecting health-promoting behavior showed that this is affected by drinking patterns, job stress, and leisure with an explanatory power of 29.7%. Based on this study, continuous support is needed for industrial workers to relieve their social gathering and job stress through leisure activities, not drinking, and we expect them to improve their quality of life through a healthy lifestyle and health promotion behavior.