• 제목/요약/키워드: miR-25

검색결과 441건 처리시간 0.031초

암환자들의 식행동과 건강식품 섭취실태에 관한 연구 (Dietary Behaviors and Consumption of Health Food among the Cancer Patients)

  • 김용신;김상연;정경아;권순형;장유경;박미현;황성주
    • 동아시아식생활학회지
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    • 제15권5
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    • pp.516-523
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    • 2005
  • This study was performed to investigate dietary behaviors and consumption of health food in cancer patients. The subjects were 163 cancer patients recruited from the general hospital in Seoul, Korea. The data were obtained by the structured self administered questionnaire. The mean age of subjects was 51 years. Most of subjects didn't eat the visual fat of meat and chicken skin. The mealtime of the subjects was generally regular and most of subjects had breakfast. The subjects of this survey liked to eat foods with sweet taste but disliked to eat hot, salty and sour taste ones. The preference for fish, marine products and vegetables was high but that for instant foods and frying foods was very low. Most of subjects consumed the foods that is generally known as anticancer foods such as vegetables and fruits. On the other hand, most of subjects didn't intake butter, margarine, and frying foods that is known as risk factors of cancer. The majority of subjects$(84.7\%)$ consumed the health food The main reasons for taking health foods by subjects were to cure disease$(58.0\%)$, to prevent disease$(45.3\%)$, to supply nutrients$(39.3\%)$, to maintain the mental state$(12.7\%)$ and to recover fatigue$(10.7\%)$. Majority of subjects$(66.0\%)$ spent money more than 200,000 won/month to buy health foods. The purchasing channels of health foods by subjects were recommendation by family or friend$(64.0\%)$, by physician/pharmacist$(18.0\%)$ and by nutritionist or dietician(6.0), and advertisement through TV or radio$(12.0\%)$. The types of taking health foods of the subjects were vegetable extracted food$(60.0\%)$, mushrooms$(51.3\%)$, Lactobacillus food$(25.3\%)$, enzyme food$(22.6\%)$ calcium containing food$(20.0\%)$ and so on. The results of the current study show that although many cancer patients already practice healthy dietary behaviors, there is a substantial proportion who do not and most of cancer patients consume health foods.' Further intervention is needed to explore the effect of health foods in cancer patients

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DNA 교차 오염 방지 기능을 가진 돼지 인플루엔자바이러스 감별진단용 one-step multiplex RT-PCR 진단법 (One step multiplex RT-PCR preventing DNA carryover contamination for differential diagnosis of swine influenza viruses)

  • 김희정;김은미;신연경;송재영;김성희;이경기;이명헌;김영화;박준철;여상건;박최규
    • 한국동물위생학회지
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    • 제37권4호
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    • pp.263-271
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    • 2014
  • In this study, we developed a cost and time saving one-step multiplex RT-PCR for the simultaneous detection and differentiation of swine influenza viruses (SIV) and 2009 pandemic influenza H1N1 virus (pH1N1). The one-step multiplex RT-PCR using four sets of primer was confirmed to be capable of detection of all SIV subtypes and differential diagnosis of major SIV subtype H1, H3 and pH1N1 on individual or mixed viral culture samples. The sensitivity of the multiplex RT-PCR was determined to be at least $2^{-6}$ $HA/25{\mu}L$ of the presented SIVs, providing sufficient efficacy for a routine SIV monitoring in diagnostic laboratories. In addition, compared with the conventional RT-PCR methods that cannot avoid the carryover DNA contamination, the developed RT-PCR applied with the uracil DNA glycosylase (UNG) system was proven to prevent a false positive reaction by carryover contamination of the pre-amplified DNA. In conclusion, the one-step RT-PCR with UNG system could be applicable to detect and differentiate of SIV from the viral cultures without worry of carryover DNA contamination in clinical laboratories.

간호대학생의 경제의식에 관한 연구 (A Study on the Consciousness of Economic Ethics in Nursing Students)

  • 홍윤미
    • 간호행정학회지
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    • 제9권3호
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    • pp.429-445
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    • 2003
  • Purpose : The present study attempted to consider the degree of consciousness of economic ethics in nursing students and the factors affecting these perceptions. Method : A survey was conducted to a total of 874 nursing students from the freshmen and seniors of 11 depts of nursing science nationwide selected by convenience sampling (one for each province, and as for Gangwon-do, two schools were selected from Yeongdong area and Yeongseo area ; 13 male students were excluded). A structured questionnaire was used to collect data on their demographic characteristics and economic ethical perceptions. Collected data were analyzed using the SAS V8.1 statistical package. Result : (1) The score for the economic ethical consciousness of the subjects was $36.76{\pm}10.20$. As for each sub-categories, the score for industry was $7.67{\pm}2.77$; thrift, $7.42{\pm}2.37$; cooperation, $7.41{\pm}2.21$; occupational consciousness, $7.18{\pm}2.20$; and, for consumption, $7.02{\pm}1.90$. The score for the consciousness of consumption was the lowest. (2) Among the demographic characteristics of the subjects, age was found to have a statistically significant positive relation to the consciousness of economic ethics(r=.13, p<.001). The next significant factor was grade: seniors seemed to have a higher economic consciousness in all the sub-categories than freshmen(t=-4.32, p<.001). The number of in-home family has a statistically significant negative correlation with economic attitudes(r=-.15, p<.001). In addition, their economic ethical perceptions were significantly higher with no religion (t=2.14, p<.05); have an unemployed father (t=2.78, p<.05); have credit cards under their own names (t=3.04, p<.05); have ever had overdue card bills (t=4.25, p<.001); have ever had part time job(t=1.74, p<.1) and when they don't live with their parents (t=-2.01, p<.05). 3) A multiple regression analysis was conducted to examine the influential power of the factors affecting the consciousness of economic ethics of the subjects. The factors had more influence on the economic attitudes of the seniors than those of freshmen; in those who having credit cards under their own names than under others; and, in those who have ever experienced credit default than those haven't. Though these factors raised average 3.0 points of economic consciousness, their expository power for the consciousness were low. Conclusion : The nursing students had medium-high consciousness of economic ethics and they seemed to have low consciousness of the proper consumption practices. Their actual life experiences had an influence on their economic attitudes. Therefore, practical programs on economic knowledge should be developed and taught to students systematically at school so that they could have sound consciousness of economic ethics and appropriate knowledge closely related with their real life.

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Serum anti-M$\ddot{u}$llerian hormone levels as a predictor of the ovarian response and IVF outcomes

  • Choi, Min-Hye;Yoo, Ji-Hee;Kim, Hye-Ok;Cha, Sun-Hwa;Park, Chan-Woo;Yang, Kwang-Moon;Song, In-Ok;Koong, Mi-Kyoung;Kang, Inn-Soo
    • Clinical and Experimental Reproductive Medicine
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    • 제38권3호
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    • pp.153-158
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    • 2011
  • Objective: The aim of this study was to investigate whether anti-M$\ddot{u}$llerian hormone (AMH) levels could be predict ovarian poor/hyper response and IVF cycle outcome. Methods: Between May 2010 and January 2011, serum AMH levels were evaluated with retrospective analysis. Three hundred seventy infertile women undergoing 461 IVF cycles between the ages of 20 and 42 were studied. We defined the poor response as the number of oocytes retrieved was equal or less than 3, and the hyper response as more than 25 oocytes retrieved. Serum AMH was measured by commercial enzymelinked immunoassay. Results: The number of oocytes retrieved was more correlated with the serum AMH level (r=0.781, $p$ <0.01) than serum FSH (r=-0.412, $p$ <0.01). The cut-off value of serum AMH levels for poor response was 1.05 ng/mL (receiver operating characteristic [ROC] curves/area under the curve [AUC], $ROC_{AUC}$=0.85, sensitivity 74%, specificity 87%). Hyper response cut-off value was 3.55 ng/mL $ROC_{AUC}$=0.91, sensitivity 94%, specificity 81%). When the study group was divided according to the serum AMH levels (low: <1.05 ng/mL, middle: 1.05 ng/mL - 3.55 ng/mL, high: >3.55 ng/mL), the groups showed no statistical differences in mature oocyte rates (71.6% vs. 76.5% vs. 74.8%) or fertilization rates (76.9% vs. 76.6% vs. 73.8%), but showed significant differences in clinical pregnancy rates (21.7% vs. 24.1% vs. 40.8%, $p$=0.017). Conclusion: The serum AMH level can be used to predict the number of oocytes retrieved in patients, distinguishing poor and high responders.

각시붕어, Rhodeus uyekii와 칼납자루, Acheilognathus koreensis에 대한 염산리도카인과 Clove Oil의 마취효과 (Effect of Lidocaine Hydrochloride and Clove Oil as an Anaesthetic on Korean Rose Bitterling, Rhodeus uyekii and Oily Bifterling, Acheilognathus koreensis)

  • 강언종;김은미;김영자;임상구;심두생;김용호;박인석
    • 한국양식학회지
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    • 제18권4호
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    • pp.272-279
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    • 2005
  • 각시붕어, Rhodeus uyekii (Mori, 1935)와 칼납자루, Acheilognathus koreensis (Kim and Kim, 1990)에 대한 염산리도카인과 Clove oil의 $10^{\circ}C,\;15^{\circ}C,\;20^{\circ}C$$25^{\circ}C$ 수온 조건에서의 마취 효과를 조사하였다. 3분 미만의 완전마취(Stage A5)와 10분 미만의 완전회복(Stage R5)을 기준시, 마취 수온 $20^{\circ}C$에서의 염산리도카인 적정 농도는 각시붕어와 칼납자루에서 각각 250${\~}$550 ppm, 150${\~}$550 ppm 이었으며 Clove oil에서는 각각 40${\~}$200 ppm, 80${\~}$240 ppm 이었다. 본 실험의 2종을 대상으로 한 염산리도카인과 Clove oil에 의한 마취시 negative곡선의 농도 의존성을 보였다. 회복시간은 마취제 농도에 따라 다소 변이를 보였으나, 일반적으로 고농도 마취는 긴 회복시간을 보였다. 마취 수온과 마취 효과는 있어 양성의 상관관계가 있어, 저온에서의 마취시 마취시간과 회복시간이 더 소요되었다. 본 연구에서 사용된 어류 마취제 염산리도카인과 Clove oil은 본 실험에 사용된 종들에 효과적인 마취제임을 시사한다. 본 연구로부터의 결과는 각시붕어와 칼납자루의 사육시 어체 진정이 요구되는 취급이나, 이들의 양식시 유용할 것이다.

GC-NPD와 GC-MSD를 이용한 농산물 중 Isotianil의 공정분석법 개발 (Development of analytical method for Isotianil in agricultural commodities using GC-NPD and GC-MSD)

  • 도정아;이미영;강일현;권기성;오재호
    • 분석과학
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    • 제25권6호
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    • pp.364-369
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    • 2012
  • Isotianil은 쌀 도열병을 방제하기 위한 새로이 개발된 식물활성 살균제로 다른 식물활성제에 비해 소량으로도 장시간의 효과를 나타내는 특징을 가지고 있다. 신규 농약 Isotianil에 대한 기준은 국내의 경우 2010년 쌀에 대하여 0.1 mg/kg으로 최초 설정되었으며, 이에 따른 식품 중 안전관리를 위해 Isotianil을 분석하기위한 검사법을 개발하였다. 검체 일정량에 acetonitrile을 가하고 균질화하여 대상농약을 추출하였으며, 액-액분배하고 florisil 카트리지를 이용한 고체상 추출 후 GC-NPD 및 GC-MSD로 분석하였다. 분석결과 농산물에 의한 간섭물질은 없었으며, 대표 농산물 현미 등 6 종에 대한 Isotianil의 분석 시험법의 정량한계와 직선성은 각각 0.05 mg/kg과 0.999($r^2$)이었다. 회수율은 대표 농산물에서 70.0~103.9%이었으며, 분석오차는 10% 미만으로 CODEX 잔류분석 기준에 적합하였다.

염장 멸치 (Salited Anchovy)의 제조조건 1. 염장방법에 따른 염장 멸치 (salted anchovy)의 제조 중 성분 변화 (Establishment of Processing Conditions of Salted Anchovy 1. Changes of Chemical Compositions during Fermentation of Salted Anchovy by Salting Methods)

  • 심길보;김태진;주정미;조영제
    • 한국수산과학회지
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    • 제34권2호
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    • pp.98-102
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    • 2001
  • Anchovy fillet 제조를 위하여 염장방법 및 숙성온도에 따른 염장 멸치의 숙성 중 성분변화를 조사하였다. 물간에 비하여 마른간 하였을 때 육의 탈수량이 많았으며, $20^{\circ}C$에 숙성한 것이 $5^{\circ}C$에 비하여 수분함량이 다소 낮았다. 염분함량은 물간한 것이 마른간에 비하여 높았으며, $20^{\circ}C$에서 숙성 한 것이 $5^{\circ}C$에 비하여 높았다. 총 질소는 물간이 마른간한 것에 비하여 낮았으며, 염장 초기에 총질소의 저하가 현저하였다. 마른간이나 물간 모두 $20^{\circ}C$에서 염장 멸치의 숙성 중 아미노태질소가 현저하게 증가하여 마른간은 숙성120일, 물간은 숙성 30일에 거의 최대를 나타낸 반면, $5^{\circ}C$에서는 미미한 증가를 보였다. 휘발성염기질소의 변화는 아미노태질소와 거의 비슷한 경향을 나타내었다. 물간한 것은 마른간에 비하여 동일 온도에서 가수분해가 빠르게 진행되었으며, $5^{\circ}C$에서는 가수분해가 현저하게 억제되었다. 과산화물가는 마른간이 물간에 비하여 빠르게 증가하였으며, $5^{\circ}C$에 숙성한 것이 $20^{\circ}C$에 비하여 낮은 과 산화물가를 나타내었다. 이상의 결과로 보면, 염장 멸치의 제조를 위해서는 대멸치를 가염지 한 다음, $25\%$의 식염으로 마른간하여 $5^{\circ}C$에서 숙성시키는 것이 육의 연화를 막고, 숙성이 어느 정도 진행된 염장 멸치론 얻을 수 있을 것으로 사료된다.

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당근발효액 첨가에 따른 닭꼬치의 품질 특성 (Quality Characteristics of Seasoned Skewered Chicken Added with Fermented Carrot Juice)

  • 김현정;황보미향;황은영;정영태;박수경;이삼빈;이인선
    • 한국식품영양과학회지
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    • 제38권8호
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    • pp.1097-1103
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    • 2009
  • 당근발효액 첨가 닭꼬치는 간장양념액과 당근발효액 5$\sim$10%을 첨가하여 숙성, 가열한 후 양념소스를 발라 재가열하여 제조한 다음, 진공 포장하여 $4^{\circ}C$에서 21일 동안 보관하면서 이화학적 및 관능적 품질특성을 조사하였다. 당근발효액은 당근액에 2% Leuconostoc mesenteroides SM를 접종하여 $25^{\circ}C$에서 18$\sim$20시간 발효시켜 제조하였다. 닭꼬치의 pH 및 색도는 각 구별 차이 없이 저장기간이 증가할수록 감소하였고, VBN값은 저장기간이 경과할수록 각 구별 차이 없이 증가하였다. 당근발효액을 첨가한 T1과 T2구는 대조구에 비해 포화지방산 함량은 감소하고 불포화지방산 함량은 증가하였으며, 총 세균이 검출되지 않았다. 또한 대조군에 비해 당근발효액이 첨가된 T1, T2구의 경도가 감소하였고, 특히 10% 당근발효액을 첨가한 T2구에서 유의적으로 낮은 경도를 확인하였다. 관능평가에서도 당근발효액이 첨가된 처리구가 높은 다즙성과 연도, 풍미에 의해 전반적인 기호도에서 높은 평가를 받았다. 따라서 당근발효액을 첨가하여 제조한 닭꼬치는 미생물의 증식 억제, 경도 저하와 함께 다즙성, 연도, 풍미 등 전반적인 기호도에서도 우수한 것으로 확인되었다.

레이더기반 다중센서활용 강수추정기술의 개발 (Development of Radar-Based Multi-Sensor Quantitative Precipitation Estimation Technique)

  • 이재경;김지현;박혜숙;석미경
    • 대기
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    • 제24권3호
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    • pp.433-444
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    • 2014
  • Although the Radar-AWS Rainrate (RAR) calculation system operated by Korea Meteorological Administration estimated precipitation using 2-dimensional composite components of single polarization radars, this system has several limitations in estimating the precipitation accurately. To to overcome limitations of the RAR system, the Korea Meteorological Administration developed and operated the RMQ (Radar-based Multi-sensor Quantitative Precipitation Estimation) system, the improved version of NMQ (National Mosaic and Multi-sensor Quantitative Precipitation Estimation) system of NSSL (National Severe Storms Laboratory) for the Korean Peninsula. This study introduced the RMQ system domestically for the first time and verified the precipitation estimation performance of the RMQ system. The RMQ system consists of 4 main parts as the process of handling the single radar data, merging 3D reflectivity, QPE, and displaying result images. The first process (handling of the single radar data) has the pre-process of a radar data (transformation of data format and quality control), the production of a vertical profile of reflectivity and the correction of bright-band, and the conduction of hydrid scan reflectivity. The next process (merger of 3D reflectivity) produces the 3D composite reflectivity field after correcting the quality controlled single radar reflectivity. The QPE process classifies the precipitation types using multi-sensor information and estimates quantitative precipitation using several Z-R relationships which are proper for precipitation types. This process also corrects the precipitation using the AWS position with local gauge correction technique. The last process displays the final results transformed into images in the web-site. This study also estimated the accuracy of the RMQ system with five events in 2012 summer season and compared the results of the RAR (Radar-AWS Rainrate) and RMQ systems. The RMQ system ($2.36mm\;hr^{-1}$ in RMSE on average) is superior to the RAR system ($8.33mm\;hr^{-1}$ in RMSE) and improved by 73.25% in RMSE and 25.56% in correlation coefficient on average. The precipitation composite field images produced by the RMQ system are almost identical to the AWS (Automatic Weather Statioin) images. Therefore, the RMQ system has contributed to improve the accuracy of precipitation estimation using weather radars and operation of the RMQ system in the work field in future enables to cope with the extreme weather conditions actively.

Successful Robotic Gastrectomy Does Not Require Extensive Laparoscopic Experience

  • An, Ji Yeong;Kim, Su Mi;Ahn, Soohyun;Choi, Min-Gew;Lee, Jun-Ho;Sohn, Tae Sung;Bae, Jae-Moon;Kim, Sung
    • Journal of Gastric Cancer
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    • 제18권1호
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    • pp.90-98
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    • 2018
  • Purpose: We evaluated the learning curve and short-term surgical outcomes of robot-assisted distal gastrectomy (RADG) performed by a single surgeon experienced in open, but not laparoscopic, gastrectomy. We aimed to verify the feasibility of performing RADG without extensive laparoscopic experience. Materials and Methods: Between July 2012 and December 2016, 60 RADG procedures were performed by a single surgeon using the da $Vinci^{(R)}$ Surgical System (Intuitive Surgical). Patient characteristics, the length of the learning curve, surgical parameters, and short-term postoperative outcomes were analyzed and compared before and after the learning curve had been overcome. Results: The duration of surgery rapidly decreased from the first to the fourth case; after 25 procedures, the duration of surgery was stabilized, suggesting that the learning curve had been overcome. Cases were divided into 2 groups: 25 cases before the learning curve had been overcome (early cases) and 35 later cases. The mean duration of surgery was 420.8 minutes for the initial cases and 281.7 minutes for the later cases (P<0.001). The console time was significantly shorter during the later cases (168.6 minutes) than during the early cases (247.1 minutes) (P<0.001). Although the volume of blood loss during surgery declined over time, there was no significant difference between the early and later cases. No other postoperative outcomes differed between the 2 groups. Pathology reports revealed the presence of mucosal invasion in 58 patients and submucosal invasion in 2 patients. Conclusions: RADG can be performed safely with acceptable surgical outcomes by experts in open gastrectomy.