• 디지털융복합연구
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• 제19권11호
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• pp.607-614
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• 2021

본 연구는 간호대학생의 전공만족과 임상실습만족이 취업스트레스에 미치는 영향을 알아보고자 시도되었다. 본 연구는 2021년 5월부터 6월까지 전라지역 소재 M대학의 4학년 간호대학생 152명을 대상으로 구조화된 설문을 통해 데이터를 수집하였다. 자료의 분석은 기술통계, t-test, ANOVA, Pearson's 상관관계 분석, 다중회귀분석을 이용하였다. 연구의 결과 간호대학생의 취업스트레스는 전공만족과는 유의한 음의 상관(r=-.356, p<.001)이 있었지만, 임상실습만족과는 상관이 없는 것으로 나타났다(r=-.018, p=.825). 다중회귀분석의 결과, 간호대학생의 취업스트레스에 영향을 미치는 요인은 나이(β=0.285, p<.001), 성적(β=0.250, p=.001), 전공만족(β=-0.331, p<.001)이었고 설명력은 25.3%였다. 본 연구의 결과로 간호대학생의 취업스트레스를 줄여주기 위해 전공만족을 높일 수 있는 정책적 프로그램의 개발과 적용이 필요하며 나이와 성적을 고려한 프로그램 개발이 필요하다.

• 전력전자학회논문지
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• 제22권4호
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• pp.285-296
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• 2017

This paper proposes an improved modulation scheme for the medium voltage modular multi-level converter (MMC), which operates in the nearest level control and applies in the medium voltage direct current (MVDC) system. In the proposed modulation scheme, the offset (neutral-to-zero output) voltage is adjusted, with the phase voltage magnitude, thereby maintaining a constant value with N+1 level in the controllable modulation index (MI) range. In order to confirm the proposed scheme's validity, computer simulations for the 22.9 kV - 25 MVA MMC were performed with PSCAD/EMTDC, as well as hardware experiments for the 380 V - 10 kVA MMC. The proposed modulation scheme offers to build a constant pole voltage regardless of the MI value, and to build a phase voltage with improved total harmonic distortion (THD).

• 한국해양생명과학회지
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• 제2권1호
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• pp.20-26
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• 2017

본 연구에서는 해산 규조류(Skeletonema cosatatum)의 개체군성장률(r)을 사용하여 중금속 3종 (As, Cr, Pb)의 독성영향 평가를 실시하였다. S. costatum을 As (0, 0.32, 0.63, 1.25, 2.50 및 5.00 mg L^-1), Cr (0, 0.16, 0.32, 0.63, 1.25 및 2.50 mg L^-1) 및 Pb (0, 1.25, 2.50, 5.00, 10.00 및 20.00 mg L^-1)에 96시간 노출한 이후에 r 값을 측정하였다. 대조구에서 r은 0.04보다 높았으며 As 농도 0.63, Cr 농도 0.63 및 Pb 농도 10.00 mg L^-1 이상에서 r이 대조구 대비 유의하게 감소하였고 중금속 농도가 증가할수록 농도 의존적으로 감소되는 경향을 나타냈다. EC₅₀(반수영향농도)을 이용한 중금속의 독성세기는 Cr>As>Pb 순서를 나타냈으며, EC₅₀ 각각 0.80, 2.57 및 13.88 mgL^-1으로 나타났으며, 최소영향농도(LOEC)는 각각 0.63, 0.63 및 10.00 mg L^-1으로 나타났다. LOEC를 기준으로 해양환경에서 Cr, As, Pb 농도가 0.63, 0.63 및 10.00 mg L^-1 이상이 되었을 때, S. costatum에게 독성 영향이 나타날 수 있을 것으로 판단된다. 또한, 본 연구를 통해 S. costatum의 r은 해양생태계 내에서 생산자 역할을 하는 생물에 대한 중금속의 독성평가를 위하여 유용하게 사용될 수 있을 것으로 판단된다.

• 한방안이비인후피부과학회지
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• 제24권2호
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• pp.68-78
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• 2011

Objective : The purpose of this clinical research was to investigate the effects of herbal cosmetics containing Momordica charntia L. extracts on the anti-wrinkle and whitening effects. Methods : A total of 25 subjects who visited Daegu Hanny Oriental Medical Center from November 1st, 2010 to December 31th, 2010 were included in this study. In this study, we observed change of R1, R2, R3, R4, R5 for using products, change of average melanin content and pigmental area, analysis results of subjective wrinkle improvement, Analysis results of subjective whitening improvement. Statistical analysis was performed by using paired t-test and wilcoxon signed ranks test. Statistical significance was achieved if the probability was less than 5%(p<0.05) Results : Statistically, herbal cosmetics containing Momordica charntia L. extracts showed siginificant effect on change of R1, R2, R3, R4, R5 for using products, change of average melanin content and pigmental area, analysis results of subjective wrinkle improvement, Analysis results of subjective whitening improvement(p<0.05). And satisfaction after using herbal cosmetics was near good. Conclusions : Considering the above results, we have concluded that herbal cosmetics containing Momordica charntia L. extracts have the remarkable effects on the anti-wrinkle and whitening effects.

• 대한본초학회지
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• 제25권4호
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• pp.47-54
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• 2010

Objectives : The original plant species of Schisandrae Fructus (O-mi-ja) is prescribed as Schisandra chinensis $B_{AILL.}$, in Korea, but S. chinensis $B_{AILL.}$ and S. sphenanthera $R_{EHD.}$ et $W_{ILS.}$ in China. Moreover, fruit of several other species in genus Schisandra also have been used as the same herbal medicines. To develop a reliable method for correct identification of Schisandrae Fructus and to evaluate the phylogenetic relationship of S. chinensis and its related species, we analyzed internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA). Methods : Twenty-four plant samples of three Schisandra species and one Kadsura species, S. chinensis $B_{AILL.}$, S. spenanthera $R_{EHD.}$ et $W_{ILS.}$, S. nigra $M_{ax.}$ and Kadsura japonica $D_{UNAL}$ were collected from each different native habitate and farm in Korea and China. The nrDNA-ITS region of each samples were amplified using ITS1 and ITS4 primer and nucleotide sequences were determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW based on the entire nrDNA-ITS sequence. Results : In comparative analysis of the nrDNA-ITS sequences, we found specific nucleotide sequences including indels (insertions and deletions) and substitutions to distinguish C. chinensis, S. spenanthera, S. nigra, and K. japonica. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origin of O-mi-ja. Moreover, we evaluated the phylogenetic relationship of four plant species by the analysis of nrDNA-ITS sequences. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterants for O-mi-ja.

• 환경생물
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• 제27권3호
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• pp.292-296
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• 2009

본 연구는 한국의 멸종위기곤충인 꼬마잠자리 알의 발육과 온도의 관계를 추정하고자 8가지 서로 다른 온도 조건(17, 20, 22, 25, 28, 30, 33 및 $36^{\circ}C$)에서 실시하였다. 꼬마잠자리의 알은 2007년 6월 경상북도 문경시 일대의 산간 습지에 서식하는 암컷 성충으로부터 채취하였다. 실험의 결과, 부화율은 17, 20, 22, 25, 28, 30, 33, 그리고 $36^{\circ}C$에서 각각 2.86, 17.09, 24.32, 39.67, 34.43, 40.57, 44.79 및 1.75%였다. 각 온도에서의 알의 발달율(평균발육속도)은 변형된 Sharpe와 DeMichele의 비선형 모형에 부합하였다. 알의 저온발육임계온도는 $14.02^{\circ}C$(y=0.005988x-0.084, $r^2$=0.99)로 추정되었고, 발육최적온도는 $30{\sim}35^{\circ}C$로 추정되었다.

• Journal of Korean Biological Nursing Science
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• 제19권4호
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• pp.215-226
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• 2017

Purpose: The purpose of this study was to investigate the effects of general, health, and alcohol-related characteristics on blood pressure and stress according to the Alcohol Use Disorders Identification Test (AUDIT). Methods: This was a descriptive study. From participants in the 2013 Korea National Health and Nutrition Examination Survey, 319 participants who responded to the survey when they were attending college or university were selected. Data were analyzed using the SPSS Win 23.0 program for composite sample data reflecting the composite design elements of stratification, colonization, and weighting. Results: Mean age of onset of alcohol drinking was $17.91{\pm}0.16$ years, the average AUDIT score was $7.56{\pm}0.41$, and 25 (9.1%) participants fell into the high-risk drinking group. In the high-risk AUDIT group, systolic blood pressure was influenced by age, gender, institution, health status, body mass index, and suicidal ideation, and the explanatory power of the model ($R^2$) was 75.0%. For diastolic blood pressure, institution and health status were the influential factors, and $R^2$ was 66.2%. Predictors of stress were health status, depression, and the age of onset of drinking, and $R^2$ was 57.2%. Conclusion: It was confirmed that the explanatory power of the variables in models of systolic and diastolic blood pressure and stress is greater in those with higher scores on AUDIT.

• 한국치위생학회지
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• 제19권2호
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• pp.317-327
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• 2019

Objectives: This study aimed to evaluate the effects of occlusion patterns using the T-scan $III^{(R)}$ system and oral parafunctional behaviors on temporomandibular joint disorder (TMD) in female college students. Methods: A questionnaire survey assessed TMD symptoms and oral behavioral checklist (OBC) scores in 120 female college students in their early twenties. Occlusion was evaluated using the T-scan $III^{(R)}$ system. Logistic regression analysis was performed to investigate the relationship between occlusion and OBC scores based on patients' symptoms of TMD and to determine whether OBC scores were a predictor of TMD. Results: A total of 101 participants with an average age of 22 (${\pm}2.17$) years were included in the analysis. The reported symptoms of TMD were categorized as moderate (28.8%), slight (27.7%), severe (25.7%), and asymptomatic (17.8%). Occlusion was analyzed using the T-scan $III^{(R)}$ system, and the mean occlusion time was 0.42(${\pm}0.5$) seconds. The results of logistic regression analysis showed that the group that would 'clench or grind teeth' during sleep showed an odds ratio for TMD that was 8.9 times higher than that in the group without this behavior. The group that would 'hold, tighten, or tense muscles without clenching' while awake showed an odds ratio for TMD that was 21.3 times higher than that in the group without this behavior. Conclusions: We confirmed that oral parafunctional behavior affects TMD. Therefore, we would like to continue studying the diagnosis and treatment of TMD by evaluating occlusion patterns and related OBC scores.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.274-279
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• 2015

Receptor activator of nuclear factor-kappa B ligand (RANKL) is a critical factor in osteoclastogenesis. It makes osteoclasts differentiate and multinucleate in bone remodeling. In the present study, RANKL was expressed as a soluble maltose binding protein (MBP)-fusion protein using the Escherichia coli maltose binding domain tag system (pMAL) expression vector system. The host cell E. coli DH5α was cultured and induced by isopropyl β-_D-1-thiogalactopyranoside for rRANKL expression. Cells were disrupted by sonication to collect soluble MBP-fused rRANKL. The MBP-fusion rRANKL was purified with MBP Trap affinity chromatography and treated with Tobacco Etch Virus nuclear inclusion endopeptidase (TEV protease) to remove the MBP fusion protein. Dialysis was then carried out to remove binding maltose from the cleaved rRANKL solution. The cleaved rRANKL was purified with a second MBP Trap affinity chromatography to separate unsevered MBP-fusion rRANKL and cleaved MBP fusion protein. The purified rRANKL was shown to have biological activity by performing in vitro cell tests. In conclusion, biologically active rRANKL was successfully purified by a simple two-step chromatography purification process with one column.

• Applied Biological Chemistry
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• 제50권2호
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• pp.87-94
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• 2007

전통 장류 발효식품의 발효도와 저장성을 증진시키기 위하여 전통 콩 발효식품으로부터 cellulase, amylase, protease 활성 및 항균활성 물질 생성이 우수한 바실러스 HS25 균주를 분리하여 그 배양학적 특성을 검토하였다. HS25 균주는 편모와 내생포자를 가지며, 다량의 점질물을 형성하는 그람양성 간균으로 catalase 양성, oxidase 음성으로 esculin을 가수분해하였고, 16S rDNA분석 등을 통하여 Bacillus subtilis로 밝혀져 B. subtilis HS25로 명명하였다. B. subtilis HS25 균주의 생육 및 항균물질 생산을 위하여 최적 배지조성은 soluble starch 1%, yeast extract 0.5%, peptone 0.5% 및 $MgCl_2{\cdot}6H_{2}O$ 0.05%이었으며, 최적 배양온도는 $25{\sim}45^{\circ}C$, 초기 pH는 $6.5{\sim}9.5$, 최적 교반속도는 $160{\sim}200$ rpm이었고, 항균활성이 가장 높게 나타나는 배양 시간 범위는 $12{\sim}36$시간째였다.