• 제목/요약/키워드: miR-155

검색결과 48건 처리시간 0.025초

Development of Kinetic Models Describing Kinetic Behavior of Bacillus cereus and Staphylococcus aureus in Milk

  • Kim, Hyoun Wook;Lee, Sun-Ah;Yoon, Yohan;Paik, Hyun-Dong;Ham, Jun-Sang;Han, Sang-Ha;Seo, Kuk-Hwan;Jang, Aera;Park, Bum-Young;Oh, Mi-Hwa
    • 한국축산식품학회지
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    • 제33권2호
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    • pp.155-161
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    • 2013
  • This study developed predictive models to evaluate the kinetic behaviors of Bacillus cereus and Staphylococcus aureus in milk during storage at various temperatures. B. cereus and S. aureus (3 Log CFU/mL) were inoculated into milk and stored at $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$, and $30^{\circ}C$, as well as $5^{\circ}C$, $15^{\circ}C$, $25^{\circ}C$, and $35^{\circ}C$, respectively, while bacterial populations were enumerated. The growth data were fitted to the modified Gompertz model to estimate kinetic parameters, including the maximum specific growth rate (${\mu}_{max}$; Log CFU/[$mL{\cdot}h$]), lag phase duration (LPD; h), lower asymptote ($N_0$; Log CFU/mL), and upper asymptote ($N_{max}$; Log CFU/mL). To describe the kinetic behavior of B. cereus and S. aureus, the parameters were fitted to the square root model as a function of storage temperature. Finally, the developed models were validated with the observed data, and Bias (B) and Accuracy (A) factors were calculated. Cell counts of both bacteria increased with storage time. Primary modeling yielded the following parameters; ${\mu}_{max}$: 0.14-0.75 and 0.06-0.51 Log CFU/mL/h; LPD: 1.78-14.03 and 0.00-1.44 h, $N_0$: 3.10-3.37 and 2.09-3.07 Log CFU/mL, and $N_{max}$: 7.59-8.87 and 8.60-9.32 Log CFU/mL for B. cereus and S. aureus, respectively. Secondary modeling yielded a determination of coefficient ($R^2$) of 0.926.0.996. B factors were 1.20 and 0.94, and A factors were 1.16 and 1.08 for B. cereus and S. aureus, respectively. Thus, the mathematical models developed here should be useful in describing the kinetic behaviors of B. cereus and S. aureus in milk during storage.

델파이 기법을 이용한 대표적인 한국의 맛과 음식에 관한 연구 (Exploring Korean Typical Tastes, Flavors and Foods Using Delphi Technique)

  • 차성미;정라나;정서진;김광옥;한귀정;이새롬
    • 한국식품조리과학회지
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    • 제26권2호
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    • pp.155-164
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    • 2010
  • The present study attempted to conceptualize Korean typical tastes, flavors and foods and to gather professionals' opinions about the globalization of Korean foods. A total of 23 experts participated in a three round survey using the Delphi technique, which was used to integrate and share the professional ideas of each expert. The survey was categorized into two parts: 1. Korean typical tastes and flavors, 2. Korean typical foods. According to the results, 'hot chili pepper', 'Kimchi', 'soy sauce', and 'garlic' were represented as Korean typical tastes and flavors. Also 'harmonized' was determined to be a type of food that should be introduced to foreigners and, 'sweet' and 'tart' were shown to also be liked by foreigners. In addition, 'neobiani', 'kalbi', 'bibimbap', 'kimchi', 'japchae', and 'neobiani' were categorized as typical Korean foods, foods that should be introduced to foreigners, and foods that would be liked by foreigners. These results showed that appropriate foods should be globalized and R&D should be expanded to determine the preferences of foreigners in terms of tastes and flavors.

가족의 지지와 제왕절개술 산모의 자아개념간의 관계연구 (A Study on the Relationship Between Perceived Family Support & Self Concept of Cesarean Section Mothers)

  • 장명자;이미라
    • 모자간호학회지
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    • 제1권
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    • pp.23-33
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    • 1991
  • Recently, Cesarean Section has been revealed as major events in maternity nursing, because it has influence on tile formatter of the self concept of cesarean section mothers as well as later coping mechanism and mother-child relationship including the mothering role. The study is conducted to clarify the relationships between the perceired family support and the self concept of cesarean section mothers, and to find out other Influencing factors on the formation of the self concept. Measuring instrument used in this study was Lee's scale to measure the self concept and the degree of perceived family support of the cesarean section mothers, The subject were 155 cesarean section mothers who were selected conveniently. Volunteers answered self reporting questionnaires which asked about their self concept. Data was analyzed as follows using SAS. The relationship between scores of the self concept and degree of the perceived family support was analyzed by Pearson's product moment correlation. In order to identify the influencing effects of demographic variables on the formation of self concept & F test were used. Result were summarized as follows : 1. Scores of self concept of cesarean section mothers were significantly correlated with scores of the perceived family support (r=0.3438, p<0.01) These results show that the family support is closely related to the self concept of cesarean mothers and has positive effects on their coping capacity. 2. Scores of self-concept of those who have lower ducation background and are multipara were significantly higher than those who have higher education background and are primipara(M=29.90$\pm$9.37, 27.69$\pm$3.89, t=2.006, df=105.8, p=0.0480: M=27.75$\pm$3.49. 29.46$\pm$8.68, t=-1.7183, 2. p=0.08).

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ANALYSIS OF THE LiF:Mg,Cu,Si TL AND THE LiF:Mg,Cu,P TL GLOW CURVES BY USING GENERAL APPROXIMATION PLUS MODEL

  • Chang, In-Su;Lee, Jung-Il;Kim, Jang-Lyul;Oh, Mi-Ae;Chung, Ki-Soo
    • Journal of Radiation Protection and Research
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    • 제34권4호
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    • pp.155-164
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    • 2009
  • In this paper, we used computerized glow curve deconvolution (CGCD) software with several models for the simulation of a TL glow curve which was used for analysis. By using the general approximation plus model, parameters values of the glow curve were analyzed and compared with the other models parameters (general approximation, mixed order kinetics, general order kinetics). The LiF:Mg,Cu,Si and the LiF:Mg,Cu,P material were used for the glow curve analysis. And we based on figure of merits (FOM) which was the goodness of the fitting that was monitored through the value between analysis model and TLD materials. The ideal value of FOM is 0 which represents a perfect fit. The main glow peak makes the most effect of radiation dose assessment of TLD materials. The main peak of the LiF:Mg,Cu,Si materials has a intensity rate 80.76% of the whole TL glow intensity, and that of LiF:Mg,Cu,P materials has a intensity rate 68.07% of the whole TL glow intensity. The activation energy of LiF:Mg,Cu,Si was analyzed as 2.39 eV by result of the general approximation plus(GAP) model. In the case of mixed order kinetics (MOK), the activation energy was analyzed as 2.29 eV. The activation energy was analyzed as 2.38 eV by the general order kinetics (GOK) model. In the case of LiF:Mg,Cu,P TLD, the activation energy was analyzed as 2.39 eV by result of the GAP model. In the case of MOK, the activation energy was analyzed as 2.55 eV. The activation energy was analyzed as 2.51 eV by the GOK model. The R value means different ratio of retrapping-recombination. The R value of LiF:Mg,Cu,Si TLD main peak analyzed as $1.12\times10^{-6}$ and $\alpha$ value analyzed as $1.0\times10^{-3}$. The R of LiF:Mg,Cu,P TLD analyzed as $7.91\times10^{-4}$, the $\alpha$ value means different ratio of initial thermally trapped electron density-initial trapped electron density (include thermally disconnected trap electrons density). The $\alpha$ value was analyzed as $9.17\times10^{-1}$ which was the difference from LiF:Mg,Cu,Si TLD. The deep trap electron density of LiF:Mg,Cu,Si was higher than the deep trap electron density of LiF:Mg,Cu,P.

초등학교 아동의 구강보건에 관한 지식과 행동의 상관관계 연구 (Interrelation Research of the Knowledge Regarding the Oral Health of the Elementary School Child and Behavior)

  • 이혜경;박정순;김미정
    • 치위생과학회지
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    • 제8권3호
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    • pp.155-161
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    • 2008
  • 본 연구는 초등학교 학생들에게 필요한 구강보건교육목표를 설정하는 데에 기초 자료를 수집할 목적으로 전라북도 익산시에 소재하고 있는 초등학교 1개교 4학년, 6학년 513명을 대상으로 하여 초등학교 학생의 구강보건에 관한 지식과 행동의 상관관계를 조사한 결과 다음과 같은 결론을 얻었다. 1. 조사대상자들의 구강보건지식 정도는 14점 만점에 8.77로 높지 않았으며, 여학생이 남학생보다 구강보건지식 정도가 높았고 유의한 차이를 보였다(p < .05). 2. 학교구강보건교육 경험이 있는 학생일수록 구강보건지식 정도도 높게 나타났으며, 통계적으로 유의한 차이를 보였다(r = 0.105, p < .05). 3. 구강보건행동 정도는 5점 Likert 척도로 조사한 결과, 전체 평균이 3.13으로 높지 않았으며, 학년에 따른 유의한 차이를 보였다(p < .05). 4. 구강보건지식이 높은 학생일수록 구강보건행동 정도가 높게 나타나, 구강보건지식과 행동의 유의한 차이를 보였다(p < .001). 5. 구강보건지식과 구강보건행동 및 학교구강보건교육 경험은 정(+)의 선형 상관이 있어 구강보건지식 정도가 높으면 구강보건행동도 높아짐을 알 수 있었다(r = 0.345, p < .001, p < .05). 6. 성별과 학년에 관계없이 학교구강보건실의 필요성과 구강건강담당 교사의 필요성은 인식하고 있었다(p < .05).

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심박변이도를 통한 폐경 전 한국인 비만 여성의 비만 관련 대사체 농도 예측을 위한 회귀분석 (Predicting the Concentration of Obesity-related Metabolites via Heart Rate Variability for Korean Premenopausal Obese Women: Multiple Regression Analysis)

  • 김종연;양요찬;이운섭;김제인;맹태호;유덕주;심재우;조우영;송미연;이종수
    • 한방재활의학과학회지
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    • 제24권4호
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    • pp.155-162
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    • 2014
  • Objectives Advanced researches on the relationship between obesity and heart rate variability (HRV), heretofore, focused on characteristics of HRV depending on the state of obesity. However, the previous researches have not quantified predictive power of HRV toward the obesity-related variables, which is rather more meaningful for clinicians who regularly treat obese patients. Hence, we designed a research to investigate whether HRV could predict serum levels of obesity-related metabolites. Methods Ninety obese premenopausal women meeting the inclusion criteria were recruited. The HRV test, blood sampling, and measurement of physical traits were conducted. Multiple regression analysis of the measurement data was carried out, putting obesity-related metabolites (insulin, glucose, triglyceride, hs-CRP, HDL, LDL, total cholesterol) as outcome variables and the others as predictors. To select appropriate predictive variables, the Akaike's Information Criterion (AIC) was applied. Normality and homoskedasticity of residuals for each model were tested to identify if there were any violations of the regression analysis's basic assumption. Logarithm transformation was used for the values of the concentration of metabolites and the HRV. Results The regression model including Total Power (TP) value and BMI had significant predictive power for serum insulin concentration (F(2, 88)=835.7, p<0.001, $R^2=0.95$). The regression coefficient of ln (TP) was -0.1002. However, it was not sure if the HRV could predict concentrations of other metabolites. Conclusions The results suggest that the Total Power (TP) value of the HRV can predict the level of serum insulin. If the BMI could be assumed as being constant, when the TP value is multiplied by n, the predicted change of insulin could be drawn by multiplying $n^{-0.1002}$. The uncertainty of this model can be assumed as approximately 5%.

청소년의 인터넷 중독 : 수면, 우울과의 관련성 (Internet Addiction in Adolescents and its Relation to Sleep and Depression)

  • 송호광;정미향;성다정;정정경;최진숙;장용이;이진성
    • 수면정신생리
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    • 제17권2호
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    • pp.100-108
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    • 2010
  • 목 적: 컴퓨터의 보급으로 인터넷 사용이 보편화되면서 인터넷 중독에 대한 관심이 증가하고 있다. 그러나 인터넷 중독의 정확한 병태생리에 대한 이해와 진단 기준은 아직 확립되어 있지 않다. 청소년은 발달 과정 중에 있기 때문에 인터넷 중독에 더 취약할 가능성이 있으며, 우울감과 수면 문제에 대한 이해가 중요하다. 본 연구에서는 청소년의 인터넷 중독, 수면 양상, 그리고 우울감의 특징과 그 연관성을 살펴보고자 하였다. 방 법:서울 시내 일 중, 고등학교의 중학교 2학년(155명), 고등학교 1, 2학년 학생(644명)을 대상으로 하였고 그 중 696명의 설문지를 분석 대상으로 하였다. 설문지에는 Young 인터넷 중독 척도(Young's Internet Addiction Scale, YI-AS), 피츠버그 수면의 질 척도(Pittsburgh Sleep Quality In-dex, PSQI), 우울감 척도(The Center for Epidemiologic Studies Depression Scale, CES-D), 그리고 인터넷 사용 패턴과 기본적인 인구학적 정보에 대한 질문들이 포함되었다. 연구 대상자들을 학년별, YIAS 점수에 따라 나누어 비교하였고, YIAS와 다른 변인과의 상관관계를 분석하였다(two-tailed, p<0.05). 결 과: 인터넷 중독군(internet-addicted, IA)은 14명(2.0%), 과사용군(over-using, ou)은 193명(27.7%), 비중독군(not-addicted, NA)군은 489명(70.3%) 이었으며, 전체 대상군의 평균 IAS 점수는 35.24점, PSQI 점수는 5.53점, CES-D 점수는 16.72점이었다. 평일 평균적인 수면 시간은 학년이 올라가면서 점점 감소하였다(F=51.909, p< 0.001). IA군, OU군, NA군으로 나누어 비교하였을 때 컴퓨터를 공부 이외의 목적으로 사용하는 시간(96.36${\pm}$63.31분 vs. 134.92${\pm}$86.79분 vs. 213.57${\pm}$136.87분, F=34.287, p<0.001), 휴대용 전자기기를 공부 이외의 목적으로 사용하는 시간(84.22${\pm}$79.11분 vs. 96.97${\pm}$91.89분 vs. 152.31${\pm}$93.64분, F=5.400, p=0.005)에 유의한 차이가 있었다. PSQI 총점(5.26${\pm}$2.97 vs. 6.08${\pm}$2.97 vs. 7.50${\pm}$4.41, F= 8.218, p<0.001)과 CES-D 점수(15.40${\pm}$8.08 vs. 19.05${\pm}$8.42 vs. 30.43${\pm}$13.69, F=32.692, p<0.001)에서도 유의한 차이가 있었다. YIAS 점수는 컴퓨터를 공부 이외의 목적으로 사용하는 시간(r=0.356, p${\pm}$0.001), 휴대용 전자기기를 공부 이외의 목적으로 사용하는 시간(r=0.136, p<0.001)과 유의한 상관관계가 있었다. YIAS 점수와 PSQI 점수(r= 0.131, p=0.001), YIAS 점수와 CES-D 점수(r=0.265, p< 0.001), PSQI 점수와 CES-D 점수(r=0.357, p<0.001)는 서로 유의한 상관관계가 있었다. 결 론: 인터넷 중독은 행위 중독의 한 가지로 여러 정신병리와 관련이 있을 뿐만 아니라, 수면의 질을 결정하는 여러 가지 요인들, 우울감과도 밀접한 관련이 있다는 것을 알 수 있었다. 따라서 청소년의 인터넷 중독을 평가할 때 동반될 우울감과 수면 양상에 대한 고려가 함께 이루어지는 통합적인 접근이 필요할 것이다.

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HPLC-UVD를 이용한 농산물 중 살충제 sulfoxaflor의 시험법 개발 및 검증 (Development and Validation of an Analytical Method for the Insecticide Sulfoxaflor in Agricultural Commodities using HPLC-UVD)

  • 도정아;이미영;박혜진;권지은;장효진;조윤제;강일현;이상목;장문익;오재호;황인균
    • 한국식품과학회지
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    • 제45권2호
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    • pp.148-155
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    • 2013
  • 국내 신규 살충제로 등록되어진 sulfoxaflor에 대해 농산물 중 잔류실태를 조사하기 위하여 HPLC-UVD와 LC-MS를 이용한 시험법을 개발하였다. Sulfoxaflor 시험법의 회수율은 시료별 적당한 추출 및 정제 방법을 적용한 결과 현미, 감자, 감귤, 사과, 배, 고추, 대두에서 0.05, 0.4 및 0.5 mg/kg 수준에서 82.8-108.2%이었다. 회수율에 대한 모든 분석오차는 10% 미만으로 CODEX 잔류물질 분석 가이드라인에 만족하였다. 분석을 위한 기기 조건에서 sulfoxaflor의 정량한계는 0.05 mg/kg이었으며 0.1-5.0 mg/kg 범위에서 상관계수($R^2$) 0.999 이상의 높은 직선성을 보여주었다. 따라서 본 연구에서 개발된 잔류농약 시험법은 농산물을 대상으로 효과적인 시료 전처리 방법과 최적의 정제 과정을 확립하여 다양한 농산물에 대한 잔류량 검출이 적당함을 확인할 수 있었다. 이와 같이 확립한 sulfoxaflor의 잔류시험법은 국제적 분석 기준을 만족할 뿐만 아니라 LC-MS를 이용한 잔류분의 재확인한 결과를 살펴볼 때 분석과정의 신뢰성 또한 확보할 수 있어 식품공전 공정시험법으로 사용될 것이다.