• Journal of The Korean Society of Clinical Toxicology
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• v.8 no.1
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• pp.1-6
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• 2010

Methylene blue is a very effective reducer of drug-induced methemoglobinemia. It has dose-dependent oxidation or reduction properties. In most cases, a dose of 1 to 2 mg/kg IV given over 5 minutes and immediately followed by a 15- to 30-mL fluid flush to minimize the local pain is both effective and relatively safe. The onset of action is quite rapid, and the effects are usually seen within 30 minutes. The dose may be repeated after 30 to 60 minutes and then every 2 to 4 hours as needed. The total dose should not exceed 7 mg/kg as a single dose or 15 mg/kg within 24 hours. Repeated treatment may be needed for treating compounds that have prolonged elimination or those compounds that undergo enterohepatic recirculation (e.g., dapsone). Methylene blue can cause dose-related toxicity. At high doses, methylene blue can also induce an acute hemolytic anemia and rebound methemoglobinemia. The reasons for treatment failure with methylene blue include ineffective GI decontamination, the existence of other forms of hemoglobin (e.g., sulfhemoglobin), a low or high dose of methylene blue and the toxicokinetics of some agents, such as aniline, benzocaine or dapsone.

• Journal of the Korean Chemical Society
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• v.39 no.1
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• pp.57-65
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• 1995

The electrochemical reduction of methylene blue (MB) in 1.0${\times}$10-2 M KNO3 aqueous solution was investigated by direct current (DC), differential pulse (DP) polarography, cyclic voltammetry (CV) and controlled potential coulometry (CPC). The electrode reduction of melthylene blue was processed CE reaction mechanism by two electrons transfer at the first reversible wave (- 0.18 volts vs. Ag/AgCl). MB was strongly adsorbed on the stationary mercury electrode and the reduction product of conptrolled potential electrolysis was rapidly auto-oxidized in air to the original methylene blue. Upon the basis of interpretation of cyclic voltammogram with pH change, possible CE electrode reaction mechanism was suggested.

• Textile Coloration and Finishing
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• v.5 no.3
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• pp.182-187
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• 1993

The binding isotherms of Methylene Blue with Poly(vinyl pyrrolidone) (PVP) were determined in a Mcllvaine buffer of pH 8.0 at 3$0^{\circ}C$ by a dynamic dialysis methods. The isotherms showed a partition binding which was increase linearly with the increase of free dye concentration in solution. The Scatchard plots for Poly(vinyl pyrrolidone)-Methylene Blue gave a constant value. The results were interpreted by the McGhee and von Hippel theory considering non-cooperative binding. The intrinsic binding constant k, for Poly(vinyl pyrrolidone)-Methylene Blue was 6.02$\ell$/base mol.

• Carbon letters
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• v.12 no.2
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• pp.85-89
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• 2011

Coloured wastewater is released as a direct result of the production of dyes as well as from various other chemical industries. Many dyes and their breakdown products may be toxic for living organisms. Activated carbon is one of the best materials for removal of dyes from aqueous solutions. The present study describes the adsorption behaviour of methylene blue dye on three microporous activated carbons, where two samples (AC-1 and AC-2) were prepared by a polymer blend technique and the other is a microporous activated carbon (ARY-3) sample from viscose rayon yarn prepared by chemical-physical activation. The effects of contact time and activated carbon dosage on decolourisation capacity have been studied. The results show that activated carbon having mixed microporosity and mesoporosity show tremendous decolourisation capacity for methylene blue. In addition, the activated carbon in the powder form prepared by the polymer blend technique shows better decolourisation capacity for methylene blue than the activated rayon yarn sample.

• The Korean Journal of Zoology
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• v.10 no.1
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• pp.27-34
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• 1967

SM 계 자성 마우스를 대조군과 실험군으로 나누어,대조군에는 0.9% 생리식염수를 , 실험군에는 methylene blue(110 mg/kg, 식염수에 용해, pH 7.0)를 각각 피하주사하고, 40 분, 6 시간 또는 24시간후에 양군을 다같이 120 r X-선으로 일시 전신조사하였다. 조사후 21 시간에 걸쳐 albumin-globulin 비, 전혈청단백질 , 그리고 체중에 대한 간 및 신장중량비를 측정하여 조사전 처리시핵에 따른 methylene blue 의 방어효과를 조사하였다. 일반적으로 양군에서 조사후 일구간에 따라 2개의 변화가 나타났으며, 첫째는 1 일구에 , 둘째 상은 8 일구에 나타났다. methylene blue 처리는 이 두 개 상 일구에서 albumin -globulin 비의 증가성, 전혈청단백질 및 간장중량의 감소성에 대하여 큰 지체성을 나타냈다. 조사전 40 분 처리군에서 특히 현저한 방어작용이 있었으나 6 시간 및 24 시간전 처리구에서는 별 영향이 없었다.

• Journal of the Korean Chemical Society
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• v.17 no.1
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• pp.53-59
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• 1973

The adsorptivity of Methylene Blue on Korean Yeongil bentonite which was premixed with a salt of KF or $NH_{4}Cl$ etc., treated at $200-500^{\circ}C$, washed and dried, was studied. In case of treatment with$NH_{4}Cl$, slight improvement of the adsorptivity of methylene blue on the products was observed. With KF, treated at$200-300^{\circ}C$, the best results was obtained. The adsorption capacity of the products was improved about 1.7 times than that of original bentonite. With $FeSO_4$ or $Na_{2}CO_3$ etc. improvement of the adsorption capacity on the products was not observed.

• Journal of The Korean Society of Clinical Toxicology
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• v.4 no.2
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• pp.151-154
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• 2006

Acute toxic methemoglobinemia is an infrequent complication of the use of various drugs. Severe methemoglobinemia is very often fatal. Methylene blue is an effective drug in the treatment of methemoglobinemia patients. However, failure to respond to methylene blue has been described in patients with sulfhemoglobinemia, chlorate poisoning, and glucose-6-phosphate dehydrogenase deficiency. It is even possible that hemolysis may occur due to methylene blue treatment itself. We encountered a case of a 71-year-old woman who developed methemoglobinemia caused by alprazolam intoxication. She presented with hemolytic anemia and did not respond to methylene blue. In spite of concerted N-acetylcysteine therapy, the hemolytic anemia became aggravated and the patient died eleven days after intoxication.

• Journal of Yeungnam Medical Science
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• v.14 no.2
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• pp.337-349
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• 1997

This study was undertaken to examine the intensity of involvement of inducible nitric oxide synthase (iNOS) and cyclic GMP signal transduction pathway as one of the mechanisms of vaso-relaxative action of bacterial lipopolysaccharide (LPS) on the canine femoral artery strips. Canine femoral arteries were isolated and spiral strips of 10 mm long and 2 mm wide were made in the Tyrode solution of $0-4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with 95% $O_2$/5% $CO_2$ at $37^{\circ}C$ and nitric oxide (NO) production was measured simulltaneously with isolated nitric oxide meter. LPS induced NO production, suppressed the phenylephrine (PE) induced contraction and enhanced the acetylcholine (ACh) induced relaxation. $N^G$-nitro-L-arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue, a guanylyl cyclase inhibitor, potentiated PE induced contraction and suppressed ACh induced relaxation on the LPS treated strips. The inhibitory potency of methylene blue for LPS induced vascular hyporesponsiveness was stronger than that of L-NAME. These results suggest that in canine femoral artery, both iNOS and cyclic GMP signal trnasduction pathway are related with LPS induced vascular hyporeponsiveness, but in minor with iNOS and in major with cyclic GMP signal trnasduction pathway.

• Applied Chemistry for Engineering
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• v.16 no.3
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• pp.366-371
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• 2005

In this study treatment efficiencies of methylene blue were evaluated in term of BOD, COD, TOC, absorbance and initial decolorization rates. Ozonation of the dye in distilled water was performed in a laboratory scale cylindrical batch reactor. The decolorization process of methylene blue was carried out by bubbling ozone at the bottom of a bubble column reactor containing the dye solution. Decolorization, determined by measuring the light absorbance at the maximum wavelength in the (${\lambda}_{max}$, 660 nm), was almost complete after 40 min with an ozone concentration of $50{\pm}10mg/L$. The $TOC/TOC_0$ ratio after ozonation was about 83.8%, the COD was diminished to 44.0% of the initial value. The $BOD_5/COD$ ratio was increased from 64.2% to about 90.8%, indicating an enhancement of biodegradable compounds in the ozonated solutions. The pseudo first-order rate constants of the ozonation was $3.30{\times}10^{-2}min^{-1}$ and the activation energy was $3.01kcal{\cdot}mol^{-1}$ at $30^{\circ}C$.

• Journal of the Korean Chemical Society
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• v.27 no.6
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• pp.411-418
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• 1983

The effects of existing anions in hydrofluoric acid solutions, $F^-$ and $HF_2$on the extraction of methylene blue-$BF_4^-$ ion-pair into 1,2-dichloroethane have been investigated by spectrophotometry. The absorbance of the extracted ion-pair is found to be independent of $F^-$ion up to $10^{-2}$ molar concentration, which implies that $F^-$ion dose not directly interfere with the extraction. However, $HF_2^-$ ion competes with $BF_4^-$ion for methylene blue and the extraction constant for methylene blue-H$F_2$ion-pair is calculated to be 8.5 at $25^{\circ}C$.