• 한국식품과학회지
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• 제11권2호
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• pp.93-98
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• 1979

본실험(本實驗)에서는 Maillard형(型) 갈색화반응(褐色化反應)의 중요(重要)한 중간생성체(中間生成體)의 하나인 pyrazine유도체(誘導體)의 항산화효과(抗酸化效果)를 보기위해 먼저 이를 주요구성성분(主要構成成分)으로 하는 methylene chloride추출물(抽出物)의 항산화효과(抗酸化效果)를 검토(檢討)하고저 했다. 즉, pyrazine유동체생성(誘導體生成)의 최적조건(最適條件)에 따라 glucose-ammonia(1M+8M)갈색화(褐色化) 반응액(反應液)을 $100^{\circ}C$에서 4시간(時間) 반응(反應)시킨후, 여기서 methylene chloride추출물(抽出物)을 얻었다. 이 추출물(抽出物)과 별도(別途)로 furfural을 식용(食用)평지씨기름기질(基質)에 첨가(添加)하여, control과 함께 $37^{\circ}C{\pm}1.0^{\circ}C$에서 저장(貯藏)하여, 그 과산화물가(過酸化物價)(POV), thiobarbituric acid value(TBA 가(價))와 산가(酸價)(AV)를 규칙적으로 측정(測定)하였다. 그리고 methylene chloride추출물(抽出物)과 furfural이 들은 기질(基質)들의 POV, TBA가(價)와 AV의 변화(變化)를 control의 그것과 비교(比較)하여 methylene chloride추출물(抽出物)과 furfural의 항산화효과(抗酸化效果)를 검토(檢討)하였다. 이상(以上)의 실험(實驗)의 결과(結果), glucose-ammonia (1M+8M)갈색화반응액(褐色化反應液)의 methylene chloride추출물(抽出物)에는 상당(相當)한 항산화효과(抗酸化效果)를, 그리고 furfural에도 다소의 항산화효과(抗酸化效果)를 확인(確認)할 수 있었다.

• 한국세라믹학회지
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• 제11권2호
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• pp.11-16
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• 1974

Korean Yung-il bentonite was treated with potassium chloride, zinc chloride, calcium chloride, ferric chloride, or chromic chloride solutions respectively varying their concentration, treating temperature and treating time. The adsorbabilities of methylene blue on these pretreated bentonite were investigated. In the case of treatment with potassium chloride solution, the improvement of the adsorbahility of methylene blue on the products was observed, and in the best result the adsorbability was 1.6 times better than that on the original bentonite. With zinc chloride solution, the optimum adsorbability was a value of 1.7 times better than that on the original bentonite. With ferric chloride, chromic chloride or calcium chloride solution, slight improvement of the adsorbability was observed.

• 한국산업보건학회지
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• 제9권1호
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• pp.87-98
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• 1999

The objectives of this study were to evaluate exposures to airborne methylene chloride and postshift carboxyhemoglobin (COHb) in blood of workers engaged in processes using blowing or cleaning agents, and to investigate correlation between methylene chloride concentrations and the blood COHb levels of workers. The geometric mean (GM) of workers' exposures (8 hour-time weighted averages, TWA) to airborne methylene chloride during cleaning molds using rags wetted with the solvent in the manufacture of flexible polyurethane foam (GM = 61.4 ppm), during operating the dip tank for cleaning molds in the manufacture of lens (GM = 61.0 ppm), and during cleaning the blowing nozzles by spraying the solvent in the manufacture of shoes (GM = 117.2 ppm) were exceeded the American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value ($TLV^{(R)}$)-Time Weighted Average (TWA) (50 ppm). The COHb levels were significantly different among groups (p<0.05). The average COHh levels in blood of non-smoking workers were 2.0% in. low-level (<50 ppm) exposure group, and 3.9 % in high-level (>50ppm) exposure group. The average COHb levels in smoking workers were 3.1% in low-level exposure group, and 4.8% in high-level exposure group. The blood COHb levels of no-exposed workers to methylene chloride were 1.8% in non-smoking group, and 2.8% in smoking group. It was found that the COHb level dependeds on the methylene chloride concentration and smoking habit, and was highly correlated with methylene chloride concentration in air. The correlation coefficient was 0.81 among non-smoking workers. The estimated COHb level (3.6%) and 95% upper confidence limit (4.0%) corresponding to TLV-TWA of methylene chloride exceeded the current ACGIH Biological Exposure Index (COHb 3.5%) for carbon monoxide. The estimated COHb level (5.4%) at 100 ppm exceeded the standard (5%) recommended by National Institute for Occupational Safety and Health (NIOSH) for preventing adverse cardiovascular effect. The estimated COHb value and 95% upper confidence limit at 25 ppm of the Occupational Safety and Health. Adminstration (OSHA) Permissible Exposure Limit-TWA (PEL-TWA) were 2.6% and 3.0%, respectively. It is suggested that COHb in blood be kept below 3.0% to comply with OSHA PEL-TWA.

• KSBB Journal
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• 제16권3호
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• pp.233-236
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• 2001

원료 의약품의 경우 정제된 최총제품 내려 잔류용매 제거는 매우 중요하다. 일반적인 진공건조 방법에 의한 제품생산시 case hardening 효과 때문에 잔류용매획 제거가 상당히 어렵다. 본 연구에서는 초임제 이산화탄소를 아용하여 정제된 paclitaxel 내의 methylene chloride와 methanol 의 잔류용매를 효과적으로 제거하였다. 초임계 이산화탄소를 이용하여 $40^{circ}C$, 80 bar에서 30분간 조업하였율 경우 최초 최종제품 내 잔류 용매인 methylene chloride 와 methanol이 각각 5,869 ppm과 2,135 ppm에서 468 ppm과 403 ppm으로 줄어 ICH 규정치를 충분히 만족시킬 수 있다. 이러한 연구 결과는 초임계 유체의 새로운 응용 분야를 개척하고 많은 원료 의약품의 잔류용매 제거에 효과적으로 사용할 수 있을 것으로 사료된다.

• Safety and Health at Work
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• 제1권1호
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• pp.98-101
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• 2010

This study explored the health hazard of those exposed to methylene chloride by assessing its atmospheric concentration when a tear gas mixture was aerially dispersed. The concentration of methylene chloride ranged from 311.1-980.3 ppm (geometric mean, 555.8 ppm), 30 seconds after the dispersion started. However, the concentration fell rapidly to below 10 ppm after dispersion was completed. The concentration during the dispersion did not surpass the National Institute for Occupational Safety and Health 'immediately dangerous to life or health' value of 2,300 ppm, but did exceed the American Conference of Governmental Industrial Hygienists excursion limit of 250 ppm. Since methylene chloride is highly volatile (vapor pressure, 349 mmHg at $20^{\circ}C$), the post-dispersion atmospheric concentration can rise instantaneously. Moreover, the o-chlorobenzylidenemalononitrile formulation of tear gas (CS gas) is an acute upper respiratory tract irritant. Therefore, tear gas mixtures should be handled with delicate care.

• 한국표면공학회지
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• 제32권2호
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• pp.109-124
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• 1999

A study for replacing methylene chloride for an alternative cleaning agent which can be utilized for cleaning aluminium parts of hard disk drives was carried out at Suwon factory of Samsung Electro-mechanics Co. Ltd. As a result, quite a good cleaning agent was selected through data collection of alternative cleaning agents, their analysis of environment effects and safty factors and testing of their cleaning power. As compared with methylene chloride, the selected cleaning agent has better cleaning power. It is also more environmental-friendly, less toxic and more economical. And the operating cost reduction due to adoption of this alternative cleaning agent in Samsung Electro-mechanics Co. Ltd. was more than ￦l46,000,000 annually without any capital instrument in addition to improvement of environmental and safty conditions in the plant.

• 대한임상독성학회지
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• 제7권1호
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• pp.41-43
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• 2009

A 52-year-old man was found dead in the driver's seat of his tank lorry. The tank lorry was used to transport industrial wastewater to a disposal plant; the material was transferred into a storage tank with the help of compressed air. The wastewater contained methylene chloride and 2-chloropyridine. No respiratory protective equipment was used while working under these conditions. The autopsy report showed extensive edema and congestion of the brain, lung, and intraperitoneal organs. The concentrations of methylene chloride in lung and brain were reported at 398 and 67 mg/kg, respectively.

• Biomolecules & Therapeutics
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• 제7권1호
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• pp.29-34
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• 1999

The cellular growth inhibition of 20 natural products was screened using SRB (sulforhodamine B) assay against 4 human cancer cell lines(SNU-1, SNU-C$_{4}$, Hep3B, Kato III). Ethanol extracts of Salvia miltiorrhiza, Saussurea lappa and Chelidonium majus showed potent anticancer activity among them, and further, it was fractionated into methylene chloride, hexane and methanol. Methylene chloride and methanol fraction of Salvia radix showed significant inhibitory activity against 4 human cancer cell lines. The effect of Salvia miltiorrhiza on anticancer activity in vitro models was evaluated with methylene chloride fraction of Salvia miltiorrhiza. Life span of ICR mice implanted with sarcoma-180 was increased by 40-61% and BDF$^{1}$ mice implanted with L1210 was increased by 66-89% upon intraperitoneal administration with methylene chloride fraction of Salvia miltiorrhiza. Based on these result, we suggested that Salvia miltiorrhiza showed anticancer activity on the in vivo and in vitro models

• 폴리머
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• 제32권5호
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• pp.483-488
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• 2008

광학용 폴리카보네이트 필름 제조에 사용되는 methylene chloride/1,3-dioxolane 혼합 용매가 폴리카보네이트 결정화에 미치는 영향에 대하여 살펴보았다. Methylene chloride에 환경 친화성 용매인 1,3-dioxolane을 혼합한 co-solvent를 용액 캐스팅 PC 필름 가공의 용매로 사용하는 경우, 혼합된 1,3-dioxolane에 의하여 필름 건조 시 용매 제거 속도가 느려져 PC필름의 결정화를 유발시킴을 알 수 있었다. 이러한 결정화 현상은 용매 조성비 및 용매 건조 온도를 조절하여 용매 제거 속도를 증가시킴으로 최소화할 수 있음을 확인하였다. PC의 결정화는 PC 필름의 표면 거칠기를 증가시키고 이는 필름의 광학 특성을 감소시키는 요인으로 작용함을 알 수 있었다.

• 한국미생물·생명공학회지
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• 제39권2호
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• pp.168-174
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• 2011

본 연구에서는 안전하고 효율적인 항진균제 개발 연구의 일환으로 73종의 약용 및 식용식물의 메탄올 추출물을 조제하고 이들의 hexane, methylene chloride, ethylacetate, butanol 분획물 및 water 잔류물 365종을 각각 조제하여 Aspergillus niger에 대한 항진균력을 조사하였다. 활성 선별단계에서 비교적 경제적이면서도 손 쉬운 순차적 유기용매 분획물을 제조하여 항진균 천연물 탐색을 시도함으로서 기존에 천연물 추출물에서 검색되지 않은 참나물, 뽕나무, 단삼의 항진균 활성을 확인하였다. 특히 항진균 활성이 보고된 바 없는 참나물의 경우 methylene chloride 분획에서 A. niger에 대한 MIC, MFC 가 각각 0.25 및 0.5 mg/mL임을 확인하여 강력한 항진균 활성을 확인하였으며, 0.25 mg/mL 농도에서 포자발아 억제활성도 나타냄을 확인하였다. 또한 활성분획은 0.5 mg/mL농도까지 인간 적혈구에 대해 용혈활성을 나타내지 않았으며, 활성물질은 당이 결합된 flavonoid로 추측되었다. 본 연구결과는 참나물 methylene chloride 분획의 우수한 항진균 활성을 제시하며, 비교적 간단한 유기 용매 분획법을 이용한 천연물 시료제조 및 이를 이용한 활성탐색이 새로운 활성물질 선별에 효과적임을 제시하고 있다.