• 생약학회지
• /
• 제35권2호통권137호
• /
• pp.134-138
• /
• 2004

A bioassay-guided fractionation of the whole extract of Viscum album var. coloratum Ohwi (Loranthaceae) led to the isolation of two triterpenoidal components; oleanolic acid (1) and ${\beta}-amyrin$ acetate (2), and a flavonoid, homoflavoyadorinin B (3) as well as large quantity of free fatty acid mixtures as active ingredients of the extract responsible for the antitumoral property. The EtOAc soluble fraction and BuOH soluble fraction of the extract demonstrated a significant inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system), and HCT-15 (colon) in vitro, whereas the remaining water soluble fraction exhibited a poor inhibition. The intensive phytochemical investigation of the EtOAc soluble fraction and BuOH soluble fraction of the extract indicated that the oleanolic acid (1) and large amounts of free fatty acid mixtures might be attributed to the in vitro antitumoral property of the whole extract of Viscum album var. coloratum.

• 한국식품저장유통학회지
• /
• 제24권6호
• /
• pp.879-884
• /
• 2017

몽골산 I. bungei를 80% 메탄올로 침지 추출하여 얻어진 추출물에 대해 n-hexane, EtOAc 및 n-BuOH의 유기용매를 활용하여 순차 분획을 실시하였고, 얻어진 결과물에 대하여 총 페놀함량, DPPH, $ABTS^+$ 라디칼 소거능 및 ${\alpha}$-glucosidase 저해활성을 평가하였다. DPPH 라디칼 소거활성은 페놀성 화합물의 함량이 높은 EtOAc 가용 분획물에서 $IC_{50}$ 값이 $148.7{\pm}1.6{\mu}g/mL$으로 우수한 DPPH 라디칼 소거 활성을 확인하였고, $ABTS^+$ 라디칼 소거활성 역시 I. bungei의 EtOAc 가용 분획물에서 $IC_{50}$ 값이 $27.8{\pm}0.9{\mu}g/mL$의 매우 우수한 라디칼 소거활성을 확인하였고, 다량 존재하는 페놀성 화합물과 라디칼 소거활성의 관련성을 나타내었다. 또한 ${\alpha}$-glucosidase 저해활성을 평가한 결과 EtOAc 가용 분획물에서 $IC_{50}$값은 $4.6{\pm}0.1{\mu}g/mL$의 강력한 저해활성을 나타내었으며, 이는 양성 대조군인 acarbose의 $IC_{50}$ 값인 $473.2{\pm}5.5{\mu}g/mL$과 비교했을 때 매우 우수한 활성을 나타내었으며, 다양한 화합물이 함유된 상태의 추출물 시료를 단일물질로 정제할 경우 더욱 우수한 활성의 화합물이 존재할 가능성을 기대하게 한다. 향후 이들 추출물의 활성물질 동정을 통한 활성 기작에 대한 연구가 필요하며, 본 연구결과로 보다 우수한 라디칼 소거활성 및 ${\alpha}$-glucosidase 저해활성을 가지는 새로운 천연 기능성 소재 발굴을 위한 기초자료로 활용 가능할 것으로 사료된다.

• 약학회지
• /
• 제25권4호
• /
• pp.153-160
• /
• 1981

The investigation aimed to study the effect of ginseng on the hepatic glutathion S-transferase activity. The ginseng methanol extract was administered to rats and mice for 10 days and their hepatic gluthatione S-transferase activities were measured by the method of Habig et al. Glutathione S-transferase activities in the rat treated with 100 and 500mg/kg ginseng methanol extract were increased by 13.4% and 17.10%, respectively and their increases were statistically significant. Similar results were also found in the mouse treated with ginseng 100mg/kg methanol extract. To investigate components of the extract which induce the enzyme, the methanol extract was fractionated into ether and butanol fraction and their effect on the enzyme was compared. Glutathione S-transferase activities in the rat treated with ether fraction were increased by 13.1%, similar to that obtained with ginseng methanol extract, whereas, butanol fraction did not show any increase in the enzyme activities. In the rats treated with maltol, one of the components in ether fraction, 5mg/kg for 10 days, activity of glutathione S-transferase was increased by 7.89%, but its increase was not significantly different from control group. Therefore, it may be concluded that ginseng methanol extract and its ether soluble fraction had effect on the elevation of glutathione S-transferase activities, whereas, butanol fraction of ginseng methanol extract had no effect on the enzyme.

• 한국식품영양과학회지
• /
• 제27권4호
• /
• pp.633-638
• /
• 1998

Fibrionolytic activity(FA) of kimchi in rat and FAs of water and methanol extracts of kimchi ingredient were determined using fibrin plate method to see where the active principles for FA are present in kimchi. Nine Sprague-Dawley per each group were fed diet containing 3, 5 or 10% of freeze dried kimchi for 6 weeks. The FA of plasma obtained from 10% kimchi group showed stronger FA than that of control. FAs of 3 and 5% kimchi group were not noticeable. FA from methanol extract of kimchi was approximately 6 times higher than that from water extract assuming that the active material may be present in a fat soluble fraction. From water extract of kimchi ingredients, dropwort, green onion, radish and baechu showed FA in descending order. And from methanol extracts, red pepper powder, radish, green onion and baechu showed FA in decending order. The Fa of methanol extract of red pepper powder was the most noticeable among samples.

• 한국자원식물학회지
• /
• 제3권1호
• /
• pp.64-70
• /
• 1990

The physicochemical activities of the underground parts of Roso rugosa Thunb (Rosaceae) , which have been used as an antidiabetic in the folkmedicines of Korea , was carried outThe methanol extract from this plant reduced serum Lipid Level the normal rat . When various fractions from the methanol extract was administered to the rat treated with 1% choholesterol and 0.5% cholic aciddiet , the ethyl acetate soluble fraction lowered the serum triglycerideand phospholipid level most remarkably.

• 한국미생물·생명공학회지
• /
• 제8권3호
• /
• pp.199-206
• /
• 1980

전보에서 분리한 균을 사용하여 당밀과 가용성 전분을 발효기질로써 구연산생산에 관한 몇 가지 실험을 한 결과는 다음과 같다. 1) 가용성전분을 기질로 하여 분리균 M-80을 표면배양할 경우 soluble starch 120g, (N $H_4$)$_2$S $O_4$3g, K $H_2$P $O_4$2g, MgS $O_4$.7$H_2O$ 0.2g, F $e^{++}$ 1.5mg, $Zn^{++}$ 1mg, methanol 20m1, D. W. 1ι, initial pH 5.0 으로 하여 8일간 배양하였을 때 61.8mg/ml의 구연산이 생성되었다. 2) 분리균 M-315를 황혈염으로 전처리한 당밀을 기질로 하여 발효조건을 구한 결과, 표면배양은 당밀 250g, N $H_4$N $O_3$0.3g, K $H_2$P $O_4$0.05g, MgS $O_4$.7$H_2O$ 0.01g, methanol 30m1, D. W. 1ι, initial PH 5.0이었고, 액내배양의 경우는 당밀 250g, N $H_4$N $O_3$0.3g, K $H_2$P $O_4$0.1g, MgS $O_4$.7$H_2O$ 0.01g, methanol 30m1, D. W. 1ι, initial pH5.0이었으며 9일 배양으로 각각 69.4mg/ml, 39.6mg/ml의 구연산이 생성되었다.

• Preventive Nutrition and Food Science
• /
• 제8권4호
• /
• pp.383-389
• /
• 2003

This study was conducted to investigate the antioxidative effects of Paeonia lactiftora Pall. (PL) seed extracts and Forsythia viridissima Lindl. (FVL) extracts on the antioxidative defense system and lipid peroxidation in the erythrocytes of rats fed a high-cholesterol diet. Sprague-Dawley male rats weighing 100 10 g were randomly assigned to nine experimental groups and fed 0.5 % cholesterol. The HC group did not receive any supplement, while the MP group was supplemented with 0.1 % methanol extract of PL seed, the MP2 group with 0.2 % methanol extract of PL seed, the EP1 group with 0.05 % ether-soluble fraction of PL seed, the EP2 group with 0.1 % ether-soluble fraction of PL seed, the MS1 group with 0.05 % methanol extract of FVL, the MS2 group with 0.1 % methanol extract of FVL, the ES group with 0.025% ethyl acetate-soluble fraction of FVL, and the ES2 group with 0.05 % ethyl acetate-soluble fraction of FVL. The experimental diets were fed ad libitum for 3 weeks. The erythrocyte SOD activity in the EP1 and EP2 groups increased 38% and 59%, respectively, when compared with the HC group, while the erythrocyte GSHpx activity in the EP1, EP2, and ES2 groups increased 30%, 31 %, and 29%, respectively, when compared with the HC group. The level of erythrocyte TBARS was significantly lower in the MP2, EP1, and EP2 groups than in the HC group, yet the level of serum TBARS was significantly lower in the all supplemented groups than in the HC group. The level of serum HDL- TBARS was significantly lower in the EP1 and EP2 groups than in the HC group, while the level of serum LDL- TBARS was significantly lower in the all the supplemented groups than in the HC group. Accordingly, the results indicated that the PL seed extracts and FVL extracts reduced oxidative damage by activating the antioxidative defense system in the erythrocytes of rats fed a high-cholesterol diet.

• Natural Product Sciences
• /
• 제9권4호
• /
• pp.210-212
• /
• 2003

In the present study Momordica dioica fruit pulp was successively extracted with hexane and methanol. To the concentrated methanolic extract ethyl acetate was added and ethyl acetate soluble portion was separated. Both hexane extract (HE) and ethyl acetate soluble portion (EASP) of methanolic extract was vacuum dried to yield the respective HE and EASP. HE and EASP were evaluated for its analgesic and anti-inflammatory activities in a dose of 50 and 100 mg/kg in mice and rats. Both HE and EASP significant exhibited analgesic and anti-inflammatory activities when compare to standard drug.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.201-202
• /
• 2001

Mass culture of microalgae as feed for mollusc, crustaceans and fish is an important components of the mariculture industry (Metting Jr., 1996) Growth activator for tetraselmis suecica were screened in methanol and water soluble extracts of several seaweed tissues. Also examined in this study were change in growth rate, biochemical composition, and digestion efficiency, of T.suecica cultured with and without the most effective of these extracts, the water soluble component extracted form M. nitidium. (omitted)

• 약학회지
• /
• 제49권5호
• /
• pp.405-409
• /
• 2005

To investigate anti-inflammatory activity of Noni extracts, we measured the phospholipase $A_2$ activity using both in vitro and in vivo system. Water soluble fraction of Noni extracts did not affect melittin-induced arachidonic acid release, whereas lipid soluble fraction inhibited it in a dose dependent manner in Raw 264.7 cells. The purified phos­pholipase $A_2$ activity was dose-dependently inhibited by lipid soluble fraction of Noni extracts but not by its water soluble fraction. Lipid peroxidation, myeloperoxidase and phospholipase $A_2$ activity in incised skin of mice were significantly increased as compared with those in non-incised skin, and these increase was attenuated by the treatment with Noni pow­der. Our data suggest that Noni extracts has anti-inflammatory activity, and this is, in part, caused by inhibitory activity of phospholipase $A_2$.