• Animal cells and systems
• /
• v.3 no.2
• /
• pp.215-219
• /
• 1999

Monoclonal antibody for delta-9-tetrahydrocannabiol (THC Ab), conjugated with protein A-gold, was employed as a probe to detect THC localization in the gland and subjacent cells of chemically fixed bracts of Cannabis. THC was detected in the outer wall of the disc cells, fibrillar matrix, the surface feature of secretory vesicles, and sheath throughout development of the secretory cavity. The probe was absent from vesicles. Label was also present in anticlinal walls of disc cells and walls of dermal and mesophyll cells. Little or no THC Ab was present in disc cells and none were detected in control tissues. This distribution pattern of THC Ab was similar to that in tissues prepared by high pressure cryofixation-cryosubstitution. Consistent association of THC with wall and wall-derived materials suggests that cannnabinoids are synthesized outside the plasma membrane and bound to a wall component, where-upon they are transported to the cavity with wall materials released from the disc cell wall during development of the secretory cavity.

• Journal of Korean Society for Atmospheric Environment
• /
• v.1 no.1
• /
• pp.25-32
• /
• 1985

To examine the effects of air pollution on Pinus thunbergii growing around Yocheon Industrial Complex, evaluation of visible injury based on Injury Index and chemical analysis of water-soluble sulfur and fluorine contents in needles were carried out on 30 sampling sites. Histological responses of needles were also investigated on the necrotic needle tissues. Not only Injury Index but water-soluble sulfur and fluorine contents were higher at all sites than those at control site, and showed significant correlationship among them. Especially, they were rather higher in the vicinity of air pollutants sources. Water-soluble sulfur contents were positively correlated with average atomospheric $SO_3$ concentrations. Microscopic examination of necrotic needle tissues showed that mesophyll cells were collapsed with hypertrophy and collapse of transfusion cells.

• Plant Disease and Agriculture
• /
• v.5 no.1
• /
• pp.50-54
• /
• 1999

Leaf blight outbreak was investigated in sweet persimmon tree orchards in Korea during a three-year period from 1995 to 1997. The man percentage of sweet perismmon leaves blighted by Pestalotiopsis theae was 15.9%. The highest disease incidence was surveyed to 20% in Milryang. The disease began from early June to late October, and peaked in September and October. The lesion size on leaf ranged mainly 1-3 cm. The typical symptoms were large grayish concentric lesions of oval patterns of either mesophyll or margin of the leaf, and olde trees were more susceptible than younger ones.

• The Plant Pathology Journal
• /
• v.27 no.2
• /
• pp.187-190
• /
• 2011

Symptoms induced in the leaves of strawberry (Fragaria x ananassa Duch.), 'Seolhyang' and 'Eyeberry', were mosaic, distortion and black colored edge on leaves at Nonsan area, one of the important production areas in Korea. Electron microscopy by quick-dip revealed the flexuous rod-shape particles having about 550-600 nm length. Cytoplasmic inclusion bodies composed of aggregated virus particles were observed frequently in mesophyll parenchyma and epidermal cells for the leaves of strawberry. The specific primers amplifying products of 635 bp and 729 bp were developed for RT-PCR detection of Strawberry mild yellow edge virus (SMYEV). Nucleotide identity of the CP gene of SMYEV was 92.8-99.2% with those of other SMYEV isolates from Gen-Bank database.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.9 no.3
• /
• pp.119-123
• /
• 1989

레드 클로버의 葉肉細胞로 부터 原形質體의 裸出에 影響을 미치는 要因들을 調査하여 다음과 같은 結果를 얻었다. 原形質體의 裸出에 使用된 酵素는 Cellulase, Macerozyme, Driselase 및 Pectolyase였으며 그 中 1%의 Cellulase ONOZUKA R-10과 0.5%의 Macerozyme R-10의 組合에서 가장 좋은 結果를 얻었다. 裸出된 原形質體의 適正 膨壓을 維持하기 爲하여 Mannitol의 濃度를 0.3~O.8M의 범위에서 6단계로 조정하였을 때, 0.7M에서 가장 높은 收率을 얻을 수 있었다. 原形質體 裸出을 爲한 酵素溶液의 pH는 5.6에서 가장 좋은 結果를 나타내었으며, 材料로 쓰인 레드 클로버 일을 4가지 크기로 區分하여 裸出實驗한 結果, 작은 잎보다 큰 잎에서 收率이 높은 傾向이었다. 酵素處理 後 裸出된 原形質體의 정제에는 0.8M의 Sucrose가 가장 適當하였다.

• Applied Microscopy
• /
• v.2 no.1
• /
• pp.33-37
• /
• 1972

The distribution, ultrastructure, and cytochemical properties of microbodies (peroxisomes) from the main roots and mature leaves of Panax ginseng were studied by electron microscopy included the activity of catalase in 3, 3'-diaminobenzidine (DAB) medium at pH 9, using glutaraldehyde-fixed tissues. The microbodies, which are about $0.5{\sim}1.5{\mu}$ diameter, were described from mesophyll cells of mature leaves and storage cells and cambial cells of main roots. The microbodies of the ginseng include the coreless, homogeneous matrix, in which catalase activities are present.

• Journal of Plant Biology
• /
• v.37 no.3
• /
• pp.285-292
• /
• 1994

Ultrastructural changes in Panax ginseng C. A. Meyer mesophyll chloroplasts and variation of thylakoid membrane protein in responce to the light intensity were studied in leaves of two-y-old plants exposed to two different light intensities under field coditions. The leaves were allowed to function for three months after emergence under two contrasting light conditions. The ginseng chloroplasts of 5% light were filled with highly stacked grana of condensely arrayed thylakoids, so that the stroma space was hardly observed. In contrast, chloroplasts from leaves at 100% sunlight had fewer thylakoid membranes and smaller grana stacks. The number of osmiophilic globules increased. Total Chl content and Chl b content were lower at 100% sunlight than 5% sunlight. The thylakoid membrane proteins in the leaves grown at 100% sunlight showed lower CPIa, LHCII and CP29 than those with 5% sunlight. This effect was most obvious for LHCII. Polypeptides showed major bands at 90, 64, 29-30, 22 and 14 kD, and minor bands at 59, 58, 54, 52, 49, 46, 44, 35, 23, 21 and 18-19 kD. All these bands were lower in intensity in the leaves exposed to 100% sunlight. Moreover, the bands at 58-59, 46-47 and 23 kD disappeared.

• Journal of Plant Biology
• /
• v.23 no.2
• /
• pp.49-54
• /
• 1980

The optimal conditions for the protoplast isolation from the leaves of pea (Pisum sativum L. cv. Sparkle) and barley (Hordeum vulgare L. cv. Baecdong) were determined in order to achieve a somatic hybridization between two species. It was revealed that the use of 0.5M sorbitol as an osmoticum was appropriate for pea. The yield of intact protoplasts was the highest (40%) when pea leaves were incubated in the enzyme solution for 4 hours. In case of barley, the optimal concentrations of cellulase, pectinase and mannitol as the enzyme solution were 2%, 1% and 0.35M, respectively. And the yield of barley protoplasts was the highest(87%) when leaves were incubated in this enzyme solution for 3.5 hours. A fusion of protoplasts from pea and barley was induced by PEG treatment enriched with calcium salts within 60 minutes.

• Journal of Plant Biology
• /
• v.15 no.1
• /
• pp.28-32
• /
• 1972

Young haploid leaf derived from the anthers of tobacco plant was cultuerd and plantlets of various ploidies were obtained. When the leaf was put on the medium supplemented with kinetin as growth regulator, plantlets developed directly from the leaf, and the plants coming out in early stage of culture were all haploid. Plants developing in later stage were mostly haploids with some exception of diploid and aneuploid. Leaves were also cultured on the callus-inducing media supplemented with 2,4-D and kinetiion, and the calluses were sub-cultured for six months. Plants developed from these calluses were mostly aneuploids of various chromosome numbers. In view of the fact that the plants directly developed from the leaf were all haploid, the tissue of the original leaf explant was assumed to be uniform as far as chromosome number was concerned. On the other hand, it seemed that the occurrence of various ploidies in the plants derived from the calluses of same origin was the result of the influence of in vitro culture. Apical meristem tissues and various multicellular bodies were formed in the epidermal and inner mesophyll tissues as well as in the sub-epidermal cells.

• Proceedings of the Botanical Society of Korea Conference
• /
• 1999.07a
• /
• pp.69-73
• /
• 1999

In plants as well as in other multicellular organisms, programmed cell death plays essential roles in the abortion or formation of specific cells and tissues during development to organize the plant [11, 15, 18]. A typical example of developmentally programmed cell death in plants is the death during differentiation of tracheary elements which are components of vessels and tracheids, a water-conducting system. The programming of cell death during tracheary element differentiation has been revealed to be unique to plant cells by using the in vitro Zinnia mesophyll cell culture system. In particular, new biosynthesis of autolysis-related enzymes such as cysteine proteases and nucleases, their accumulation of the vacuole and the programmed collapse of the vacuole are essential to the death of tracheary elements and differ greatly from the process of the apoptotic cell death in animals.