• Title/Summary/Keyword: mesocotyl length

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Genetic analysis of photoblastism and mesocotyl elongation

  • Lee, Hyun-Sook;Kang, Ju-Won;Jeon, Yun-A;Ahn, Sang-Nag
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.26-26
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    • 2017
  • Seed germination stimulated by light is said to be photoblastism. Photoblastism has not been reported in cereal crops, especially in the rice, but Korean weedy rice was reported to have photoblastism and longer mesocotyl than cultivar. Photoblastic weedy rice (PBR) was used to identify QTLs for photoblastism and mesocotyl length. In previous works, QTLs for photoblastism, pbr1 and pbr12 were identified on chromosomes 1 and 12 using 124 F4 lines from a cross between Ilpum and PBR using bulked segregant analysis. Two QTLs for mesocotyl elongation, qMel-1 and qMel-3 were mapped on chromosomes 1 and 3 120 F8 lines from the same cross. Of interest, the RM8260-RM246 region of pbr1 overlapped with a region of qMel-1. To know whether these two QTLs are functionally related, 110 F3 lines were developed from a cross between Ilpum and CR7124. CR7124 having photoblastism and long mesocotyl was selected from 120 F8 lines. 95 F3 lines were measured for germination rate in a light and dark condition and mesocotyl length. Mesocotyl length and germination rate in the dark condition in F3 lines showed significant correlation (r = 0.7, P < 0.0001). 95 $F_3$ lines were genotyped with RM7419 on chromosome 1. ANOVA showed that RM7419 was tightly linked to QTLs for photoblastism as well as mesocotyl length on chromosome 1 (P < 0.0001) indicating the tight linkage of two QTLs. Fine mapping of the two QTL is underway to analyze their functional relationship.

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Morpho-Physiological Studies on Elongation of Mesocotyl and Seminal Root in Rice Plant II. Effects of Seed Treatment and Soil Moisture Content on Mesocotyl Elongation (수도 중배축 종근 생장의 형태.생리학적 연구 II. 종자처리와 토양수분이 유묘의 중배축 신장에 미치는 영향)

  • 김진호;이성춘;송동석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.4
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    • pp.325-330
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    • 1989
  • This study was undertaken to assess the effects of seed treatment and soil moisture content on mesocotyl elongation in rice seedlings. The effect of the high temperature pre-treatment on the mesocotyl elongation was 7 times as great as the control (non treatment). The mesocotyl lengths were of maximum value at the 8% soil moisture, but of minimum value at the 16% soil moisture plot, showing decreasing trend of mesocotyl length as soil moisture increases. The mesocotyl elongations were much greater at the ABA treated plot than at the GA$^3$, IAA and Kinetin plots. The relationship between the mesocotyl and excised root elongations was not evident, but the weight of excised roots became heavier with longer mesocotyl length.

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Morpho-Physiological Studies on Elongation of Mesocotyl and Seminal Root in Rice Plant I. Varietal Differences and Effects of Seed Maturity and Storage Condition on Mesocotyl Elongation (수도 중배축 및 종근 생장의 형태.생리학적 연구 I. 중배축 신장의 품종간 차이와 종자숙도 및 저장조건의 영향)

  • 김진호;정병관;이성춘
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.3
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    • pp.296-302
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    • 1989
  • The mesocotyl elongation, having much effect on emergence and stand establishment in the direct sowing culture of rice, was investingated and summarized as follows: As to origin of cultivars, the average lengths of mesocotyl were 9.6 mm in the Indica-Japonica hybrid cultivars, 4.4 mm in the native cultivars and 3.2mm in the Japonica type, respectively. The mesocotyl lengths were tjereh, aman, aus, boro and bulu in order, with the mesocotyl lengths of 29.3mm and 5.4 mm in tjereh and bulu, respectively. The mesocotyl lengths were great in Baekkyungjo, Dadajo & Hejo among the native cultivars, in Sangpungbyeo, Paltal & Gokyangdo of the Japonica type and in Weonpungbyeo, Gayabyeo, Milyang 30 & Sujeongbyeo in Indica- Japonica hybrid cultivars, respectively. The mesocotyl length was the greatest at the seeds sampled I week after flowering (39. 3mm in length), and became decreased with the longer grain-filling. The mesocotyl elongated worse with the longer duration of seed storage regardless of seed maturity, and became longer at the 5$^{\circ}C$ storage plot than at the 15 and 25$^{\circ}C$ plots.

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GWAS analysis and selection of useful resources for direct-seeding related mesocotyl elongation in rice

  • Park, So-Yeon;Lee, Ah-Rim;Wang, Heng;Son, Tae-Soo;Ryu, SuNoh;Kwon, Soon-Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.151-151
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    • 2017
  • In Asia, rice production has some difficulties with reduction of farm household population and increase of elderly population. As a result, it has resulted in inefficiency and we needs to reduce labor force and improve labor productivity. Direct-seeding in rice could reduce labor and production costs, the area of direct seeding is increasing in japonica rice production in Asia. In direct seedling cultivation competition against weeds is one of most important concern. So, low temperature germinability and mesocotyl elongation should be considered. In this study, we evaluated the mesocotyl length and low temperature germination conducted association analysis using 137 korea core collections. An average length of mesocotyl among 137 core collections was skewed range from 0mm to 43mm. we searched candidate gene around target SNP. Such related traits, genome-wide association study (GWAS) analysis was carried out using GAPIT. Also, average mesocotyl length of 394 korea landrace cultivars was measured ranging from minimum 0 mm to maximum 34mm. 30 out of 394 Korea landrace cultivar conducted re-sequencing, and haplotype analysis of candidate gene. we searched these related resources, which including germination of low temperature and mesocotyl elongation. This could be used for the development of direct-seeding cultivars. The valiated accession of core collection and landrace cultivars will be used development of direct-seedling cultivar in the future.

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Seedling Emergence of Dry-seeded Rice Varieties under Two Soil Moisture Regimes (건답직파에서 토양수분조건에 따른 벼 품종의 출아특성)

  • 이변우;명을재
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.5
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    • pp.502-511
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    • 1994
  • Investigated were the relationships between plumule elongation characteristics and seedling emergence of 98 varieties including native, improved and red rice varieties of Korea, and varieties from U.S.A., Italy, Japan, etc. under proper and excess soil moisture regimes. Under excess soil moisture condition the seedling emergence and establishment rates were much lower in most of the varieties and the seedling emergence rates were delayed substantially in all varieties employed. The seedling emergence rates were lowest in dwarf and some semi-dwarf varieties showing poor plumule elongation under proper soil moisture condition. The seedling emergence and establishment rates were significantly correlated with the length of mesocotyl, coleoptile and incomplete leaf under both soil moisture regimes, and with the lower first and second internode length under excess soil moisture condition. Average emergence duration showed significant negative correlation with the length of mesocotyl, coleoptile and incomplete leaf under both soil moisture regimes and significant positive correlations with the first and third lower internode length. Based on the standardized partial regression analysis only the variations of mesocotyl and coleoptile length contributed significantly to the varietal difference in seedling establishment rate, and so did the variations of mesocotyl and incomplete leaf length to the varietal differences in emergence speed. In conclusion varieties with greater elongation characteristics of mesocotyl, coleoptile and incomplete leaf are desirable for higher and speedy seedling establishment in dry-direct seeding.

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Effect of Seeding Depth on the Growth, Mesocotyl Elongation and Herbicidal Response of Rice and Barnyardgrass (파종심도(播種深度)에 따른 벼와 피의 생장(生長), 중경(中莖) 신장(伸長) 및 제초제반응(除草劑反應) 차이(差異))

  • Chon, Sang-Uk;Guh, Ja-Ock
    • Korean Journal of Weed Science
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    • v.15 no.1
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    • pp.19-29
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    • 1995
  • The effect of seeding depth on the growth, mesocotyl elongation and herbicidal response of rice(Oryza sativa L., Japonica type, cv. "Tongjin") and barnyardgrass(Echinochloa crus-galli Beauv. var orizicola Ohwi.) were studied in greenhouse experiments. Barnyardgrass growth as affected by different water depths was briefly tested. Rice and barnyardgrass were broadcast in soil into Oem, 1cm, 2cm and 3cm in seeding depth under dry direct-seeded condition. Butachlor(N-(buthoxymethyl)-2-chloro-N-(2,6-diethylphenyl) acetamide) at dose rate of 1800g ai/ha and thiobencarb(S-[(4-chlorophenyl)methyl] diethyl carbamothioate) at dose rate of 2100g and 4200g ai/ha were soil applied to them at 5 days after seeding. At 10 days after seeding, plants harvested to examine their growth as affected by seeding depth. Root length and shoot fresh weight of rice untreated was greatest in 1cm- and 3cm-seeding depth, respectively, however, mesocotyl did not elongate. While plant height of barnyardgrass grew regardless of seeding depth and water depth, but root length was greatest in 1cm-seeding depth of dry condition and reduced with increased water depth. And mesocotyl was elongated in only dry condition and its length increased with increased seeding depth. At 10 days after application thiobencarb applied pre-emergence inhibited plant height, and shoot fresh weight of rice in only 0cm-seeding depth under dry condition whereas was unaffected in above 1cm-seeding depth, similar to untreated control, and ever increased root length and root fresh weight of rice. On the other hand, shoot, root and leaf growth of barnyardgrass was, severely inhibited regardless of application rates and seeding depths. Reduction of shoot growth by treatment of herbicide was significantly greater than that of root growth. Consequently, reduction of barnyardgrass growth by treatment of thiobencarb did not be associated with seeding depth and mesocotyl elongation as affected by different seeding depth.

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Antidoting Effect of 1,8-Naphthalic Anhydride on Butachlor Phytotoxicity in Rice (벼에 있어서 Butachlor 약해(藥害)에 미치는 1, 8-Naphthalic Anhydride 의 약해경감효과(藥害輕減效果))

  • Chun, J.C.;Hwang, I.T.;Han, M.S.
    • Korean Journal of Weed Science
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    • v.5 no.2
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    • pp.169-174
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    • 1985
  • Antidoting effect of 1,8-naphthalic anhydride (NA) on butachlor [2-chloro-2', 6'-diethyl-N-(butoxyethyl) acetanilide] phytotoxicity in rice was determined at different seeding depths and application rates of butachlor. The most sensitive response to NA was found in mesocotyl of rice (Oryza saliva L.). The mesocotyl length decreased with use of NA when seeded 2 to 4 cm deep, whereas no effect was obtained in plant height, root length and coleoptile length. Phytotoxic effect of butachlor to rice sown in vermiculite saturated with butachlor solutions decreased with use of NA at all seeding depths employed. However, depth protection was not observed when planted 3 to 4 cm deep without use of NA. Use of NA resulted in reduction in the phytotoxicity at concentrations of butachlor lower than 40 ppmw. The results indicated that the antidoting effect of NA was not due to reduction of mesocotyl elongation which would result in decrease in butachlor uptake through the mesocotyl.

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Seedling Emergence and Initial Growth between Water and Dry Seeding in Forage Millet (사료용 피의 건답과 무논 파종 시 입모 및 초기생육특성)

  • Ahn, Seok-Hyeon;Chung, Nam-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.1
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    • pp.83-88
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    • 2014
  • This research was carried out to determine the characteristics of germination in relation to temperature, and seedling emergence and growth affected by soil moisture in forage millet. The seeds of forage millet could germinate at more than $15^{\circ}C$ at the rate of 0, 90.5, 97.3 and 96.8% respectively 10 days after seeding at 10, 15, 20 and $25^{\circ}C$. The result showed that the temperature needs to be $15^{\circ}C$ or more for the seeds to germinate. The effects of seeding depth on the growth characteristics and emergence of millet seed were investigated in waters and direct seeding methods by thoroughly mixing the seed and soil. In water treatment, the emergence rates were 3.7% at $15^{\circ}C$, 7.6% at $20^{\circ}C$, 6.3% at $25^{\circ}C$, while direct seeding treatment showed the emergence rates of 65.6, 75 and 71% at 15, 20 and $25^{\circ}C$, respectively. At 15, 20, and $25^{\circ}C$, seeds in water seeding could germinate at 0.5, 1.8, 1.5 cm of soil depth, while in direct seeding, they could germinate at the depth of 9.5, 10.0, 9.9 cm. When the initial growth characteristics of the seeds were investigated, there was positive correlation between seeding depth and mesocotyl length in both water and direct seed methods. Growth and development in terms of leaf age, root length, coleoptiles and mesocotyl length, and plant height were found maximum at 0.5 cm soil depth for water seeding, and 3-5 cm soil depth for direct seeding. Results showed that there were close relationship among temperature, soil depth and moisture acquiring methods, and the combination of these factors greatly affected the initial growth characteristics and development of millet seeds. It can be concluded that, to get good seedling stand and germination of millet seed, millet should grown in field condition at the depth of 0.5 cm or less for water seeding, and 3-5 cm for direct seeding method.

Seedling Emergence of Dry -seeded Rice under Different Sowing Depths and Irrigation Regimes (건답직파에서 파종심도와 관개조건에 따른 벼 품종들의 출아특성)

  • 이변우;명을재
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.59-68
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    • 1995
  • Investigated were the relationships between plumule elongation characteristics and seedling emergence of 46 varieties including native, improved and red rice varieties of Korea, and varieties from U.S.A., Italy, India, Japan under 1, 3, and 5cm deep sowing with irrigated and non-irrigated condition. Experiments were carried out in paddy field of sandy loam. There was heavy shower of 19.2mm on the next day of seeding and thereafter, clear and dry weather continued during the experiment period. Soil temperature averaged over 30 days after seeding was $16.4^{\circ}C$ at 3cm depth. Soil hardness increased linearly up to 2.5kg /$cm^2$ on the 14th day after seeding, on which date irrigated plot was irrigated through furrow, and up to 4kg / $cm^2$ on the 28th day in non-irrigated plot. Soil hardness dropped near to 0kg /$cm^2$ after irrigation and developed up to 2.5kg /$cm^2$ again by 28 days after seeding. Seedling emergence was higher in irrigated plots than non-irrigated plots at all seeding depths. Korean improved varieties were substantially lower in seedling emergence under non-irrigated condition of 1 cm deep sowing than those under irrigated condition. This poor seedling emergence resulted mainly from delayed emergence by exposing them to greater soil strength. Percent seedling emergence under irrigated and non-irrigated condition showed signifi-cant correlations at 3 and 5 cm deep sowing. Korean improved varieties belonged to the group of poor seedling emergence, and I taliconaverneco, Chinsura Boro and Weld Pally to best group under both irrigation conditions at 3 and 5cm deep sowing. Seedling emergence showed highly signifi-cant positive correlation with the plumule length of mesocotyl + 1st internode + incomplete leaf and of mesocotyl+coleoptile. Among the characters constituting plumule length, incomplete leaf length showed greatest positive correlation followed by coleoptile and mesocotyl under irrigated condition at 3 and 5 cm deep sowing, and highest correlation with mesocotyllength followed by first internode and incomplete leaf under non-irrigated condition. Days to 50% seedling emergence at 1 cm deep sowing with irrigation showed great varietal variation of 10 to 30 days, and showed high significant negative correlations with percent seedling emergence under both irrigation conditions except for 1 cm deep sowing with irrigation, Days to seedling emergence revealed sig-nificant negative correlations with plumule characters except 2nd internode, showing highest cor-relation with incomplete leaf length.

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Effect of Soil Strength on Seedling Emergence of Rice and Barnyardgrasses in Direct Dry-Seeding (건답직파에서 토양경도가 벼와 피의 출아에 미치는 영향)

  • Kwon, Yong-Woong;Lee, Byun-Woo;Kim, Do-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.489-495
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    • 1996
  • Seedling emergences of four rice varieties (Dongjinbyeo, Dadajo, Galsaekggarak-sharebyeo, and Italiconaverneco) and three barnyardgrass species(Echjnochloa oryzjcola, E. crus-gali var. crus gali E. crus-gali var. praticola) were evaluated in relation to soil strength. Soil strength was varied by compressing the entire volume of soil with a hydraulic jack so as to be 0.5, 1, 2, 3, 6kg /$\textrm{cm}^2$. Soil strength was measured with a penetrometer (Yamanaka type) and soil covering above the seed was 4cm deep. Experiments were conducted at two air temperature conditions of 17 and $25^{\circ}C$. At a soil strength of up to 2kg/$\textrm{cm}^2$, little or no decrease in seedling emergence occurred in all rice varieties and barnyardgrasses tested. Above that value, seedling emergence decreased progressively as the soil strength increased. The degree of decrease was greatest in Dongjinbyeo and smallest in Dadajo among tested rice varieties, and greatest in Echinochloa oryzicola among barnyardgrasses, being greater in barnyardgrasses than rice. Seedling emergence was delayed almost linearly as the soil strength increased. The delay was greatest in Dongjinbyeo among rice varieties and in Echinochloa oryzicola among barnyardgrasses. Mesocotyl length increased as soil strength increased up to 2 to 3kg / $\textrm{cm}^2$ in Dongjinbyeo and Dadajo in 17$^{\circ}C$ and $25^{\circ}C$, and up to 6kg/$\textrm{cm}^2$ in Galsaekggaraksharebyeo and Italiconaverneco in $25^{\circ}C$. Dongjinbyeo showed the least elongation of mesocotyl among rice varieties in any soil strength. The total length of mesotyl, first internode and incomplete leaf showed little variation with soil strength. The total length was longer than the 4cm covering depth in other varieties except Dongjinbyeo. This might have caused the lower emergence rate in Dongjinbyeo than other varieties in higher soil strength.

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