• 한국대기환경학회지
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• 제9권1호
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• pp.44-50
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• 1993

The suspended particulate matters had been collected by a cascade impactor having 9 size fragnated stages for 2 years (Dec. 1989 to Nov. 1991) in Kyung Hee University-Suwon Campus. The particulate matters were then collected on each stage by membrane filters. The weight concentration on each stage was estimated by a microbalance and further chemical element concentrations were determined by an XRF system. The study focused on developing of a receptor model using the TTFA (target transformation factor analysis) to apportion aerosol mass with the 30 ambient data sets. Each data set consists of the 8 size-ranged subdata sets characterized by 13 element variables. Even though a couple of sources could not be identified, three to five sources were extracted from each aerosol size-range, and then 9 statistical source profiles were generated. Finally, source contribution of particulate matters on each size-range could be estimated in Suwon area.

• 미생물학회지
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• 제38권4호
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• pp.250-250
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• 2002

This study confirmed that white rot fungus Irpex lacteus was able to metabolize 2,4,6-trinitrotoluene (TNT) with two different initial transformations. In one metabolic pathway of TNT a nitro group was removed from the aromatic ring of TNT. Hydride-Meisenheimer complexes of TNT (H/sup -/-TNT), colored dark redo were confirmed as the intermediate in this transformation by comparison with the synthetic compounds. 2,4-Dinitrotoluene as a following metabolic product was detected, and nitrite produced by denitration of $H^-$-TNT supported this transformation. In the other TNT pathway, nitro groups in TNT were successively reduced to amino groups via hydroxylamines. Hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes were identified as the intermediates. The activity of a membrane-associated aromatic nitroreductase was detected in the cell-free extract of I. lacteus. This enzyme catalyzed the nitro group reduction of TNT with NADPH as a cofactor, Enzyme activity was not observed in the presence of molecular oxygen.

• Journal of Microbiology
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• 제38권4호
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• pp.250-254
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• 2000

This study confirmed that white rot fungus Irpex lacteus was able to metabolize 2,4,6-trinitrotoluene (TNT) with two different initial transformations. In one metabolic pathway of TNT a nitro group was removed from the aromatic ring of TNT. Hydride-Meisenheimer complexes of TNT (H$\^$-/-TNT), colored dark redo were confirmed as the intermediate in this transformation by comparison with the synthetic compounds. 2,4-Dinitrotoluene as a following metabolic product was detected, and nitrite produced by denitration of H$\^$-/-TNT supported this transformation. In the other TNT pathway, nitro groups in TNT were successively reduced to amino groups via hydroxylamines. Hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes were identified as the intermediates. The activity of a membrane-associated aromatic nitroreductase was detected in the cell-free extract of I. lacteus. This enzyme catalyzed the nitro group reduction of TNT with NADPH as a cofactor, Enzyme activity was not observed in the presence of molecular oxygen.

• 구강회복응용과학지
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• 제25권3호
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• pp.237-242
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• 2009

악관절부위의 활액성 연골종증은 매우 드물기 때문에 확진하기 까지 종종 몇 개월 몇 년이 걸리도 한다. 활액성 연골종증은 드문 양성 병소로 활액 내 수 많은 연골성, 골연골성 결정과 관절강내의 소성체가 형성되는 질환으로 관절강 내에 소성체를 형성하는 활액막의 연골성 변성으로 정의할 수 있다. 본 증례에서 29세 여자 환자로 저작시 동통을 주소로 내원하였다. 방사선 소견상 상관절강내에 석회화된 소성체가 발견이 되었다 치료는 석회된 소성체와 활액막 일부를 제거하였다. 술 후에 환자의 저작시의 동통이 완전히 사라졌다. 현재 아무런 재발 증상을 보이지 않고 있다.

• Applied Microscopy
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• 제31권2호
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• pp.199-206
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• 2001

완두 자엽세포에 대한 저장단백질 과립의 발달은 단백질 저장 액포의 가장자리에 축적된 단백질의 fragmentation에 의해서 이루어지는 것으로 알려져 왔다. 그러나 최근 이 외에도 terminal dilation, transformation, denovo development등의 독립적인 과정이 각각 다른 시기에 관찰되므로서 단백과립의 발달은 이러한 여러 과정이 모두 나타나는 복합과정임이 알려졌다. 이러한 과정과는 별도로 종자발달의 이른시기에 소포체 내강에 축적되는 저장단백질과 여기서 발달하는 단백과립에 대하여 규명하고자 발달중인 종자의 자엽으로부터 단일 세포를 얻어서 면역세포 화학적 반응을 실시하였다. 그 결과 종자발달의 이른 시기에는 legumin이, 중간시기 이후 에는 vicilin이 축적되므로서 단백질이 축적된 소포체가 단백과립으로 발달하는 것으로 나타났다 소포체 내강에 존재하는 단백질인 $\alpha-Tip$은 비교적 늦은시기에, toneplast membrane protein인 PPase는 이른시기에 각각 면역 세포화학적 반응이 관찰되었다.

• Molecules and Cells
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• 제42권2호
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• pp.113-122
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• 2019

Communications at the interface between the apical membrane of follicular cells and the follicular lumen are critical for the homeostasis of thyroid gland. Primary cilia at the apical membrane of thyroid follicular cells may sense follicular luminal environment and regulate follicular homeostasis, although their role in vivo remains to be determined. Here, mice devoid of primary cilia were generated by thyroid follicular epithelial cell-specific deletion of the gene encoding intraflagellar transport protein 88 (Ift88). Thyroid follicular cellspecific Ift88-deficient mice showed normal folliculogenesis and hormonogenesis; however, those older than 7 weeks showed irregularly dilated and destroyed follicles in the thyroid gland. With increasing age, follicular cells with malignant properties showing the characteristic nuclear features of human thyroid carcinomas formed papillary and solid proliferative nodules from degenerated thyroid follicles. Furthermore, malignant tumor cells manifested as tumor emboli in thyroid vessels. These findings suggest that loss-of-function of Ift88/primary cilia results in malignant transformation from degenerated thyroid follicles.

• 대한환경공학회지
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• 제35권2호
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• pp.144-150
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• 2013

본 연구에서는 고급산화공정에서 촉매제로 사용되는 산화철 나노입자를 세라믹 멤브레인 표면에 부착하여 오존 산화 공정과 연계 처리가 가능한 반응성 세라믹 멤브레인을 합성하고, 이를 이용한 하이브리드 세라믹 멤브레인 시스템(일원화된 오존-멤브레인 시스템)을 통해 자연유기화합물에 의한 막 오염 제어 특성을 평가하였다. 디스크 형태의 알루미나 정밀여과 및 한외여과 세라믹 멤브레인에 소결법을 사용하여 산화철 나노입자를 부착하였으며, 산화철 나노입자 양에 따른 반응성 세라믹 멤브레인의 특성을 분석하였다. 주사전자현미경(SEM) 분석을 통해 세라믹 멤브레인 표면 위에 산화철 나노입자 층이 형성되었음을 확인할 수 있었고, 부착된 산화철 나노입자의 크기는 대략 50 nm임을 확인할 수 있었다. 반응성 세라믹 멤브레인과 기존 세라믹 멤브레인의 막 투과 성능(Pure water permeability) 비교 실험 결과 큰 차이를 보이지 않았는데, 이는 반응성 세라믹 멤브레인 표면에 형성된 산화철 나노입자 층이 멤브레인의 투과 유량에 큰 영향을 끼치지 않음을 확인할 수 있었다. 하이브리드 세라믹 멤브레인 시스템을 통한 자연유기화합물의 막 오염(Fouling) 및 막 오염 회복(Fouling recovery) 실험을 통해, 반응성 세라믹 멤브레인을 사용한 시스템이 산화철 나노입자와 오존과의 반응을 통해 생성된 수산화라디칼이 보다 효율적으로 자연유기화합물을 분해하여 막 오염을 저감하는 것을 확인할 수 있었다. 또한 원수와 처리수 내의 자연유기화합물 분석을 통해, 반응성 세라믹 멤브레인 시스템이 보다 효과적으로 자연유기화합물의 방향성 성분 감소, 고분자량 비율 감소, 소수성 성분 감소 등을 통해 막 오염을 제어함을 확인할 수 있었다.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.747-758
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• 2002

The skin displays a highly active metabolism of polyunsaturated fatty acids (PUFA). Dietary deficiency of linoleic acid (LA), an 18-carbon (n-6) PUFA, results in characteristic scaly skin disorder and excessive epidermal water loss. Although arachidonic acid (AA), a 20-carbon (n6) PUFA, is metabolized via cyclooxygenase pathway into predominantly prostaglandin $E_2(PGE_2)$ and $PGF_{2{\alpha}}$, the metabolism of AA via the 15-lipoxygenase (15-LOX) pathway, which is very active in skin epidermis and catalyzes the transformation of M into predominantly 15S-hydroxyeicosatetraenoic acid (15S-HETE). Additionally, the 15-LOX also metabolizes the 18-carbon LA into 13S-hydroxyoctadecadienoic acid (13S-HODE), respectively. Interestingly, 15-LOX catalyzes the transformation of $dihomo-{\gamma}-linolenic$ acid (DGLA), derived from dietary gamma-linolenic acid, to 15S-hydroxyeicosatrienoic acid (15S-HETrE). These monohydroxy fatty acids are incorporated into the membrane inositol phospholipids which undergo hydrolytic cleavage to yield substituted-diacylglycerols such as 13S-HODE-DAG from 13S-HODE and 15S-HETrE-DAG from 15S-HETrE. These substituted-monohydroxy fatty acids seemingly exert anti-inflammatory/antiproliferative effects via the modulation of selective protein kinase C as well as on the upstream/down-stream nuclear MAP-kinase/AP-1/apoptotic signaling events.

• Clinical and Experimental Reproductive Medicine
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• 제14권2호
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• pp.93-100
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• 1987

To determine the effect of calcium on the preimplantational development of mouse two-cell embryo, the various concentrations of calcium were added into the culture media and the rate of blastocyst formation was observed. Also, to examine the effect of trifluoperazine, an inhibitor of calmodulin which is involved in the several intracellular calcium functions, embryos were cultured for 48 hours at the various concentrations of this inhibitor. An additional 24 hour culture was done to examine the effect of this drug on the transformation from morula to blastocyst. The results are as following ; 1. About 1.71mM of extracellular calcium is adequate for blastocyst formation and the higher concentrations of calcium (3.43mM and 8.55mM) do not affect on the blastocyst formation and the degenerating rate. 2. Trifluoperazine $100{\mu}M$ presents the inhibitory effect on the blastocyst formation while $1{\mu}M$ and $10{\mu}M$ do not so. 3. After an additional 24 hour culture, there is transformation of morula to blastocyst and the degenerating rate of embryo is increased all together.

• 대한두개안면성형외과학회지
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• 제22권2호
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• pp.126-130
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• 2021

Sebaceous neoplasms are rare adnexal tumors that can present a challenge to clinicians. Only four cases of sebaceous carcinoma with sebaceoma have been reported in the literature. Herein, we describe the case of a sebaceous carcinoma originating from a sebaceoma in a solitary nodule of the posterior neck. Immunohistochemically, the tumor cells were strongly positive for epithelial membrane antigen and p53. It is possible that adnexal carcinomas may arise from malignant transformation of their benign counterparts as well as de novo. Malignant transformation was likely in this case because the lesion was composed of distinct benign and malignant components, and the benign component showed the typical histopathological features of sebaceoma. This case underscores the fact that partial and superficial biopsies sometimes may not provide the correct diagnosis. If a surgeon suspects malignancy based on a clinical examination, then it is mandatory to perform a deep biopsy.