• Toxicological Research
• /
• v.12 no.2
• /
• pp.155-161
• /
• 1996

The enzymatic properties for NADPH-P450 reductase domain of a fusion protein between human cytochrome P450 1A1 and rat NADPH-P450 reductase expressed in Escherichia coli were investigated. The fusion plasmid pCW/1A1OR-expressed E. coli membrane showed high NADPH-cytochrome c reductase activity ($830.1\pm 85.8 nmol\cdot min^{-1}\cdot mg protein^{-1}$), while pCW control vector and P 450 1A1 expression vector pCW/1A1 showed relatively quite low activity ($4.35\pm 0.49, 3.27\pm 0.50 nmol\cdot min^{-1}\cdot mg protein^{-1}$, respectively). The kinetic curves for NADPH-cytochrome c reductase followed typical Michaelis-Menten kinetics. The $K_{max}$ and $V_{max}$ for NADPH-dependent reductase activity were $8.24\pm 2.61\mu $and $817.9\pm 60.8 nmol\cdot min^{-1}\cdot mg protein^{-1}$, respectively, whereas those for cytochrome c-dependent reductase activity were $19.97\pm 2.86\mu M$ and $1303.5\pm 67.1 nmol\cdot min^{-1}\cdot mg protein^{-1}$. The reductase activities were also compared with those of rat, porcine and human liver microsomes. The activity of pCW/ 1A1OR-expressed E. coli membrane was 15.2-fold higher than that of rat liver microsome. Treatment with benzo(a)pyrene, 7-ethoxyresorufin and $\alpha$-naphthofiavone which are known as specific substrates or inhibitor for human P450 1A1 increased NADPH-cytochrome c reductase activity of fusion protein in E. coli membrane dose-dependently. These results demonstrate that the membrane topology of fused enzyme may be important for activity of its NADPH-P450 reductase domain.

• The Korean Journal of Physiology and Pharmacology
• /
• v.13 no.1
• /
• pp.39-47
• /
• 2009

Gaegurin 4(GGN 4), an antimicrobial peptide isolated from a Korean frog, is five times more potent against Gram-positive than Gram-negative bacteria, but has little hemolytic activity. To understand the mechanism of such cell selectivity, we examined GGN4-induced $K^+$ efflux from target cells, and membrane conductances in planar lipid bilayers. The $K^+$ efflux from Gram-positive M. luteus(2.5 ${\mu}g/ml$) was faster and larger than that from Gram-negative E. coli(75 ${\mu}g/ml$), while that from RBC was negligible even at higher concentration(100 ${\mu}g/ml$). GGN4 induced larger conductances in the planar bilayers which were formed with lipids extracted from Gram-positive B. subtilis than in those from E. coli(p<0.01), however, the effects of GGN4 were not selective in the bilayers formed with lipids from E. coli and red blood cells. Addition of an acidic phospholipid, phosphatidylserine to planar bilayers increased the GGN4-induced membrane conductance(p<0.05), but addition of phosphatidylcholine or cholesterol reduced it(p<0.05). Transmission electron microscopy revealed that GGN4 induced pore-like damages in M. luteus and dis-layering damages on the outer wall of E. coli. Taken together, the present results indicate that the selectivity of GGN4 toward Gram-positive over Gram-negative bacteria is due to negative surface charges, and interaction of GGN4 with outer walls. The selectivity toward bacteria over RBC is due to the presence of phosphatidylcholine and cholesterol, and the trans-bilayer lipid asymmetry in RBC. The results suggest that design of selective antimicrobial peptides should be based on the composition and topology of membrane lipids in the target cells.

• Proceedings of the KIPE Conference
• /
• 2008.06a
• /
• pp.458-461
• /
• 2008

This paper deals with a single-phase impedance-source dynamic voltage restorer (Impedance-DVR) to mitigate voltage sag/swell for the critical loads. The proposed system is composed of passive filter and impedance-source topology inverter. As an ESS(Energy Storage System) of the proposed system is employed the Proton Exchange Membrane Fuel Cells (PEMFC). To calculate and control the compensation voltage, single-phase $^id-^iq$ theory in dq rotating reference frame and PI controller are used. Simulation results under voltage sag and swell are presented to show the performance.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.41 no.12
• /
• pp.940-949
• /
• 2013

In this paper, wrinkle reduction design was studied for triangular sail. Wrinkles in the solar sail membrane can change the load path and surface topology which may have an adverse effect on propulsion performance and controllability. In this study, wrinkle reduction strategies of adjusting cable angle, catenary and catenary-wire schemes were considered and the design parameters for wrinkle reduction were systematically investigated.

• Proceedings of the KIEE Conference
• /
• 2005.10c
• /
• pp.329-333
• /
• 2005

In this study paper, a 3[kW] Fuel Cell Generation (FCG)system with Fuel Cell(FC)simulator has been proposed. The developed FC simulator generates the actual voltage and current output characteristics of the Proton Exchange Membrane Fuel Cell (PEMFC), so that the overall performance and the dynamics of the proposed system could be effectively examined and tested. In this paper, at first, the system configuration and operational principle of the developed FC simulator has been investigated and the design process of the FCG system is explained in detail. In addition, the validity of the proposed system has been verified by the informative simulation and experimental results.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.15 no.1
• /
• pp.75-85
• /
• 2010

In this paper, a Z-source hybrid active power filter is proposed to compensate the harmonics and reactive power in power distribution system. The proposed system is composed of a 7th harmonics-tuned passive filter and an active power filter with a Z-source inverter topology, while voltage source PWM inverter or current source PWM inverter are applied as the power converter topology of conventional active power filters. The Z-source impedance network along with shoot through capability would ensure a constant DC voltage across the DC link. A polymer electrolyte membrane fuel cell is employed as an compensation DC energy source of the proposed system and its equivalent R-L-C circuit is modeled for simulation. As the compensation and control algorithm of the proposed system, the current synchronous detection algorithm is applied. The simulation analysis by PSIM is performed under the three-phase 220V/60Hz voltage source and 25A nonlinear diode loads. The effectiveness of the proposed the system is verified in the steady and transient states.

• Journal of the Microelectronics and Packaging Society
• /
• v.13 no.1 s.38
• /
• pp.57-61
• /
• 2006

Fuel cells are direct current (DC) power generators. They generate electricity through an electrochemical process that converts the energy stored in a fuel directly into electricity. Fuel cells have many benefits, which produce no particulate matter, nitrogen or sulfur oxides. And they have few moving parts and produce little or no noise. When fueled by hydrogen, they yield only heat and water as byproducts. Their wide application can reduce our dependence on fossil fuels and foreign sources of petroleum. This paper is studied on a high efficiency power conditioning system (PCS) applied to the proton exchange membrane fuel cell (PEMFC) generation system. This paper is designed to a novel PCS circuit topology of high efficiency. Some experimental results of the proposed PCS is confirmed to the validity of the analytical results.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.22 no.10
• /
• pp.41-48
• /
• 2008

This paper proposes a three-phase Z-source dynamic voltage restorer (Z-DVR) to mitigate the voltage sag for the critical loads. The proposed system is composed of passive filter and Z-source topology inverter. As an ESS(Energy Storage System) of the proposed system is employed the Proton Exchange Membrane Fuel Cells (PEMFC). To calculate and control the harmonics and compensation voltage, $i_{d}-i_{q}$ theory in dq rotating reference frame and PI controller are used. In case that three-phase voltage sags occurred, a PSIM simulation was done for the performance comparison of the conventional method employed battery stacks and proposed method. As a result, considering the voltage compensation performance, each method was nearly similar. Also, the compensation performance and the %THD(%Total Harmonic Distortion) result under the various source voltage conditions (sag or swell) were presented and discussed to show the performance of the proposed system.

• BMB Reports
• /
• v.32 no.4
• /
• pp.409-413
• /
• 1999

The cDNA encoding CMP-NeuAc:lactosylceramide ${\alpha}2$,3-sialyltransferase (GM3 synthase) was isolated from a human fetal brain cDNA library using sequence information obtained from amino acid sequences found in the conserved regions of the previously-cloned mouse GM3 synthase (mST3Gal V) and human sialyltransferases. The cDNA sequence included an open reading frame coding for 362 amino acids, and the primary structure of this enzyme predicted all the structural features characteristic of other sialyltransferases, including a type II membrane protein topology and both sialylmotifs. Comparative analysis of this cDNA with mST3Gal V showed 85% and 86% identity of the nucleotide and amino acid residues, respectively. The expression of this gene is highly restricted in both human fetal and adult tissues.

• Journal of Microbiology
• /
• v.44 no.1
• /
• pp.83-91
• /
• 2006

The sudden appearance and potential lethality of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in humans has resulted in a focusing of new attention on the determination of both its origins and evolution. The relationship existing between SARS-CoV and other groups of coronaviruses was determined via analyses of phylogenetic trees and comparative genomic analyses of the coronavirus genes: polymerase (Orflab), spike (S), envelope (E), membrane (M) and nucleocapsid (N). Although the coronaviruses are traditionally classed into 3 groups, with SARS-CoV forming a $4^{th}$ group, the phylogenetic position and origins of SARS-CoV remain a matter of some controversy. Thus, we conducted extensive phylogeneitc analyses of the genes common to all coronavirus groups, using the Neighbor-joining, Maximum-likelihood, and Bayesian methods. Our data evidenced largely identical topology for all of the obtained phylogenetic trees, thus supporting the hypothesis that the relationship existing between SARS-CoV and group 2 coronavirus is a monophyletic one. Additional comparative genomic studies, including sequence similarity and protein secondary structure analyses, suggested that SARS-Co V may bear a closer relationship with group 2 than with the other coronavirus groups. Although our data strongly suggest that group 2 coronaviruses are most closely related with SARS-CoV, further and more detailed analyses may provide us with an increased amount of information regarding the origins and evolution of the coronaviruses, most notably SARS-CoV.