• 대한한방부인과학회지
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• 제22권4호
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• pp.28-45
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• 2009

Purpose: This study was performed to elucidate the inhibitory effect of Yukmijihwangtanghapyijihwangagambang (YM) on melanin synthesis in B16F10 melanoma cells. Methods: To demonstrate the inhibitory effects of YM on melanin synthesis, we measured the amount of released and produced melanin in B16F10 melanoma call. Also, we evaluated tyrosinase-activity in vitro as well as in B16F10 melanoma call. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2, PKA, $PKC{\beta}$, ERK-1 ERK-2, AKT-1 and MITF in B16F10 melanoma call. Results: 1. YM decreased the release and production of melanin in B16F10 melanoma cells. 2. YM decreased tyrosinase activity in vitro and in B16F10 melanoma cells. 3. YM decreased the expression of tyrosinase, TRP-1, TRP-2 in B16F10 melanoma cells. 4. YM decreased the expression of PKA, $PKC{\beta}$ in B16F10 melanoma cells. 5. YM increased the expression of ERK-1, ERK-2 and AKT-1 in B16F10 melanoma cells. 6. YM decreased the expression of MITF in B16F10 melanoma cells. Conclusion: From these results, it may be concluded that YM has antimelanogenetic effects.

• 대한암한의학회지
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• 제21권1호
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• pp.35-40
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• 2016

Objectives : choroidal melanoma is a rare solid tumor and has been known that thers is few effective drugs for the treatment. The purpose of this case report is to report a case of reduction of choroidal melanoma-related eye pain and headache treated with Seganmeongmok-tang. Methods : A patient with choroidal melanoma-related eye pain and headache was treated with Seganmeongmok-tang. The changes in clinical findings were evaluated. Results : The symptoms of patients were improved after about 1 week taking Seganmeongmok-tang. Conclusion : This case report suggests that Seganmeongmok-tang may have an effect on reduction of choroidal melanoma-related eye pain and headache. Further rigorous studies are necessary to investigate the therapeutic effects of Seganmeongmok-tang on choroidal melanoma.

• 대한세포병리학회지
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• 제7권2호
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• pp.185-191
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• 1996

Metastatic melanoma is often difficult to diagnose morphologically. However, the prognostic and therapeutic decision in cases of metastatic melanoma depends upon the morphologic documentation of the metastatic disease, which may rapidly and accurately be done by cytologic methods such as aspiration cytology of clinically suspicious lesion. Cytological features of 12 metastatic melanoma are described. Evaluation for melanin pigment, background composition, cytoplasmic vacuoles, intranuclear inclusions, bi- or multinucleated cells, eccentric nuclei, nucleoli, and degree of cellular cohesion were made. the presence of melanin pigment, prominent nucleoli, intranuclear cytoplasmic inclusions, lack of cell cohesion, and eccentric nuclei are the important and useful cytologic features in the diagnosis of metastatic melanoma. Bi or multinucleated cells, cytoplasmic vacuoles, and bloody background are also frequently found in smears from metastatic melanoma.

• KSBB Journal
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• 제13권6호
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• pp.718-723
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• 1998

The effect of glycyrrhizin on melanoma cells was investigated. DNA fragmentation in cultured melanoma cells was promoted by the addition of glycyrrhizin in a dose dependent manner. Administration(i.m.) of glycyrrhizin to Mel/ret transgenic mice resulted in apoptosis induction, reduction of mitochondrial transmembrane potential in melanoma cells. Decreased B220+ B cells were recovered by the treatment of glycyrrhizin in splenocytes and mesenteric lymph node cells, while Thy-1+ T cells were not influenced. Results suggested that glycyrrhizin acted as an inducer of apoptosis of melanoma cells and an immuno-potentiator via recovered B lymphocyte population in Mel/ret transgenic mice.

• 한국임상수의학회지
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• 제37권5호
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• pp.282-285
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• 2020

A 14-year-old neutered-male Shih-Tzu was referred for glaucoma and hyphema accompanied by systemic hypertension. After topical corticosteroid and antiglaucoma medications, the left eye (OS) progressed phthisically, but ocular hypertension redevelop one-year-and-seven-months later. Suspected limbal melanoma developed in peripheral cornea/limbal sclera with heavily pigmented cornea OS. Ultrasound biomicroscopy and ocular ultrasonography differentiated ciliary body origin-heterogeneous mass from limbal mass. Persistent hyphema and pigmented cornea might obscure intraocular lesion and advanced diagnostic methods played a crucial role. Unlike the generally benign limbal melanoma, aggressive treatment was recommended for uveal tumor. Histopathology of enucleated OS confirmed malignant uveal melanoma with chronic hypertensive vasculopathy.

• 약학회지
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• 제47권6호
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• pp.404-409
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• 2003

To investigate the effect of chitosan oligosaccharide on melanin synthesis, we measured tyrosinase activity and melanin production in B16 melanoma cells. Chitosan oligosacchaide itself did not have any anti-oxidant activity in DPPH radical scavenging, and did not affect the proliferation of B16 melanoma cells. Chitosan oligosaccharide dose-dependently increased melanin production in the absence or presence of MSH. However, chitosan oligosaccharide did not have any influence on the tyrosinase activity and tyrosinase expression in B16 melanoma cells. These results suggest that chitosan oligosaccharide-induced melanin production may be independent on tyrosinase activity in B16 melanoma cells. From the above results. chitosan oligosaccharide dose-dependently appears to increase melanin production in B16 melanoma cells, suggesting that chitosan oligosaccharide may be used as a tanning agent.

• 임상이비인후과
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• 제29권2호
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• pp.259-263
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• 2018

There are many treatment options for the malignant melanoma. Wide excisional surgery is one of the most acceptable treatments for locoregional treatment. Depending on the pathologic classification, however, some other treatment option can be included such as chemotherapy, radiotherapy and immunotherapy as adjuvant treatment. Small cell type malignant melanoma is a rare variant of malignant melanoma. It is known that melanomas manifesting this morphology are invariably in vertical growth phase and have an aggressive course. The authors encountered small cell type malignant melanoma and would like to share the experience of successful treatment with surgery plus immunotherapy as one of adjuvant treatment options.

• 한국멀티미디어학회논문지
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• 제25권8호
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• pp.1203-1211
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• 2022

Melanoma is a skin cancer that starts in pigment-producing cells (melanocytes). The death rates of skin cancer like melanoma can be reduced by early detection and diagnosis of diseases. It is common for doctors to spend a lot of time trying to distinguish between skin lesions and healthy cells because of their striking similarities. The detection of melanoma lesions can be made easier for doctors with the help of an automated classification system that uses deep learning. This study presents a new approach for melanoma classification based on an ensemble of deep convolution neural networks and a Log-Gabor filter. First, we create the Log-Gabor representation of the original image. Then, we input the Log-Gabor representation into a new ensemble of deep convolution neural networks. We evaluated the proposed method on the melanoma dataset collected at Yonsei University and Dongsan Clinic. Based on our numerical results, the proposed framework achieves more accuracy than other approaches.

• 대한화학회지
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• 제67권3호
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• pp.181-190
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• 2023

Melanoma is a malignant skin tumor caused by damage to melanocytes that can spread to other organs. Hence, various studies have been conducted on preventing the spread of melanoma. Flavonoid-structured substances such as apigenin and galanzin are effective therapeutic agents for inhibiting the proliferation and metastasis of melanoma. In this study, luteolin, quercetin, and their respective derivatives were synthesized. These compounds inhibited cell proliferation of B16 melanoma cells. These results confirmed that the derivatives of quercetin and luteolin may be useful as therapeutic agents to prevent melanoma metastasis.

• Obstetrics & gynecology science
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• 제61권6호
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• pp.698-701
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• 2018

We report a rare case of vaginal amelanotic melanoma. Malignant melanomas are cutaneous and extracutaneous tumors that arise from embryological remnants of neural crest cells/melanocytes. Amelanotic melanomas at such rare locations can be misdiagnosed both clinically and radiologically. Therefore, histopathological examination and immunohistochemistry are mandatory for the diagnosis of these tumors. We diagnosed this case using histopathology and confirmed the diagnosis based on the presence of immunohistochemical markers human melanoma black 45 (HMB45) and S-100.