• The Korean Journal of Zoology
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• v.23 no.1
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• pp.13-24
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• 1980

The germarium contains the apical complex consisting of a multinucleated syncytium. Apical complex has a nutritive role for the early spermatogonia. Trophocytes are irregularly shaped and are distributed throughout the space from spermatogonial cysts to sperm bundles, and they increase in size by endopolyploidization. As meiotic divisions are not complete until the final moult, spermatogenesis continues even in the adult. Chromosome number is 2n=24. Spermiogenesis is divided into seven stages in terms of chromatin concentration, cytoplasmic granules, and the nuclear size and shape. These stages could be grouped into the spherical, the elongating and the rodform spermatids in shape.

• Development and Reproduction
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• v.4 no.1
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• pp.79-85
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• 2000

This study was made to optimize the conditions needed to produce two types of gynogenetic diploids in the sweet fish, Plecoglossus altivelis. Firstly, ultraviolet (UV) ray doses between 3,000 erg to 14,000 erg/$\textrm{mm}^2$ were tested to inactivate sperm genetically. Based on the appearance of the haploid syndromes in the embryo, a dose of UV ray 6000~7000 erg was required to inactivate sperm genetically. Then, cold shock treatment at 1~2$^{\circ}C$ for 15~30 min were conducted to retain the 2nd polar body in inseminated egg. The best elapsed time before the start of the cold shock was examined between 5~8 min. The experiments in which began 5 min after insemination at 1~2$^{\circ}C$ during 17.5 min gave 21.2％ survival rate and 89.7% normal eyed embryo rate. The gynogenetic diploid produced by suppression of the first cleavage, a considerably high number of heteroploids appeared and high mortality was observed at the metamorphosis stage, so further investigation is needed. The production of gynogenetic diploids were confirmed by GPI isozyme marker. The heterozygous type in Gpi-1 locus was observed in the meiotic-G2N as a result of gene-centromere recombination during meiosis. The heterozygous type was never observed in mitotic-G2N and showed segregation into two homozygous types at Gpi-1 locus.