• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.21-21
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• 2003

An efficient plant regeneration system was developed for Hordeum vulgare L. cv Baegdong - an important Korean cultivar. The protocol was based on a series of experiments involving the sizes of mature embryos and the culture media. The embryo size is found to be critical for the establishment of embryogenic callus. Embryos of 1.1-1.5 mm size showed a much higher ability to produce embryogenic callus capable of regenerating green plants. The auxins picloram and dicamba proved effective in inducing callus from mature embryos. 2.5 mg $I^{-1}$ dicamba and 4.0 mg $I^{-1}$ picloram in Murashige and Skoog's (MS) medium was optimum for the induction of primary callus. The induced primary callus was loose and friable which ultimately developed into creamy white and compact callus after transferring into the fresh medium. Multiple shoots were induced in the MS medium supplemented with 6.0 g $I^{-1}$ maltose, 20 mg $I^{-1}$ sorbitol, 0.5 mg $I^{-1}$ 2,4-D and 1.0 mg $I^{-1}$ kinetin and the rate was 6.5 shoots per embryo. Regenerated plants were hardy and developed roots rapidly in the medium containing 0.2 $I^{-1}$ IBA. This efficient plant regeneration system provides a foundation for generating transgenic plants of this important barley cultivar.

• Journal of the Korea Safety Management & Science
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• v.16 no.3
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• pp.351-362
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• 2014

A prerequisite for improving the quality of a product is to enhance the quality of its parts supplied from outsourced enterprises. An enterprise certified by ISO 9001 Quality Management System is expected to develop their effective quality assurance system and maintain it through continuous control and improvement, thereby ensuring the good quality of products that they produce. However, it is reported that small and medium-sized enterprises certified by ISO 9001 have difficulty establishing an effective quality assurance system. This study aims to propose a new method of improving a quality assurance system that can be more effective in small and medium-sized enterprises. This study collectively considered requirements specified in several international standards on quality system and the results of expert group brainstorming to identify evaluation areas and items for assessing a quality assurance system. Based on these evaluation areas and items, a set of checklists for diagnosing and improving a quality assurance system was developed. In order to demonstrate the usefulness of the proposed method, this study applied it to ten small and medium-sized enterprises and could identify four approaches for improving their current quality assurance system. The presented improvement method, which consists of establishment of quality goals and quality innovation plan, defective products corrective activities, 4M and design change process, improvement of customer quality information management, is expected to be utilized as a useful tool for the enhancement of the quality assurance system of small and medium-sized enterprises.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.456-460
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• 2016

Biofertilizers are increasingly available in the market as one of the alternatives to chemical fertilizers. The supply of a high number of viable microorganisms is important for farmers. Lysobacter capsici YS1215 producing chitinases and gelatinases, isolated from soil in Korea, was evaluated for the establishment of an optimal medium condition of its shelf life under an in vitro condition. In this study, the population density of a biofertilizer (L. capsici YS1215) in media containing crab shell and gelatin powder (M1, M2, M3 and M4) was observed to be higher than that of populations grown in TSB (Tryptic soy broth) media (M5, M6 and M7) during experimental period. In addition, the population density at 11 months was over $10^6\;CFU\;mL^{-1}$ in M1, M3 and M4, but under $10^6\;CFU\;mL^{-1}$ in M2, M5, M6 and M7. The best optimal medium for the shelf life was M1 ($2.6{\times}10^6\;CFU\;mL^{-1}$) containing both chitinous and gelatinous materials at 11 months. Therefore, this study provided results of the appropriate medium composition for the enhancement of the shelf life of L. capsici YS1215.

• Archives of design research
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• v.15 no.3
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• pp.191-200
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• 2002

In the 1980s, even though Italy faced economic depression, Italy (she), now, internationally became a strong nation with its best design by national unity and the developmental leap of industry through design. In Italy, especially, small and medium enterprises are accumulated by the same business in each area on the structure of a Cluster system. Italy has some characteristics that being the Italians' elastic way of management, family management and development of a consortium of small and medium enterprises, which have flexibility, imagination and decisiveness. We have to bring these intensified factors of design competitiveness to our country so that we should form the union in order to have a monopoly of specific enterprises based on the specialized technology of small and medium enterprises innovating the structure of a small quantity production having developmental flexibility. We also heighten the sense of designer and publics by achieving social opinion and cult! ural revival. Moreover, the mind of managers has to be changed more aggressively. Finally, the actual policy of design will be established and peformed, and design-company (enterprises) and the industrial economy will generally develop when we activate the structure of the industry centered on small and medium enterprises and design-related association that is united and participates in the establishment of design policy.

• Journal of the Korean Institute of Gas
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• v.3 no.1
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• pp.8-13
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• 1999

The article 49-2 of Industrial Safety Law requires that all the owners of industrial plants which contains dangerous facilities perform the process safety management. That is, this law requires the owners of industrial plants to take necessary measures to prevent fire, harmful gas leaking, explosion, and other serious accidents that could cause demage and injuries to the employees. So far, domestic chemical plants have tried to invest money and time in safety management. But, such efforts have been made only in chemical plants that were subsidiaries of large business groups. Moreover, since the economic crisis of Korea which is symbolized by the IMF bailout, small and medium size companies could not afford to invest in safety management. Their major concern is to increase productivity and thereby, survive in this crisis. The goal of this research is to develop the process safety management system that can help small and medium size companies to positively secure the process safety management. So, in developing the process safety management system, the financial and practical difficulties of such companies are fully taken into consideration.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10a
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• pp.59-67
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• 2003

An efficient plant regeneration system was developed for Hordeum vulgare L. cv Baegdong - an important Korean cultivar. The protocol was based on a series of experiments involving the sizes of mature embryos and the culture media. The embryo size is found to be critical for the establishment of embryogenic callus. Embryos of 1.1-1.5 mm size showed a much higher ability to produce embryogenic callus capable of regenerating green plants. The auxins picloram and dicamba proved effective in inducing callus from mature embryos. $2.5\;m;I^{-1}$ dicamba and $4.0\;mg\;I^{-1}$ picloram in Murashige and Skoog's (MS) medium was optimum for the induction of primary callus. The induced primary callus was loose and friable which ultimately developed into creamy white and compact callus after transferring into the fresh medium. Multiple shoots were induced in the MS medium supplemented with $6.0\;g\;I^{-1}$ maltose, $20\;mg\;I^{-1}$ sorbitol, $0.5\;mg\;I^{-1}$ 2, 4-D and $1.0\;mg\;I^{-1}$ kinetin and the rate was 6.5 shoots per embryo. Regenerated plants were hardy and developed roots rapidly in the medium containing $0.2\;I^{-1}$ IBA. This efficient plant regeneration system provides a foundation for generating transgenic plants of this important barley cultivar.

• Journal of Microbiology and Biotechnology
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• v.32 no.9
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• pp.1178-1185
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• 2022

Steroids are a class of compounds with cyclopentane polyhydrophenanthrene as the parent nucleus, and they usually have unique biological and pharmacological activities. Most of the biosynthesis of steroids is completed by a series of enzymatic reactions starting from cholesterol. Synthetic biology can be used to synthesize cholesterol in engineered microorganisms, but the production of cholesterol is too low to further produce other high-value steroids from cholesterol as the raw material and precursor. In this work, combinational strategies were established to increase the production of cholesterol in engineered Saccharomyces cerevisiae RH6829. The basic medium for high cholesterol production was selected by screening 8 kinds of culture media. Single-factor optimization of the carbon and nitrogen sources of the culture medium, and the addition of calcium ions, zinc ions and citric acid, further increased the cholesterol production to 192.53 mg/l. In the 5-L bioreactor, through the establishment of strategies for glucose and citric acid feeding and dissolved oxygen regulation, the cholesterol production was further increased to 339.87 mg/l, which was 734% higher than that in the original medium. This is the highest titer of cholesterol produced by microorganisms currently reported. The fermentation program has also been conducted in a 50-L bioreactor to prove its stability and feasibility.

• Korean Journal of Construction Engineering and Management
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• v.13 no.1
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• pp.95-105
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• 2012

The government introduced the technical proposal drafting system in September 2007, as a new method to stimulate technical competence among construction companies to require ingenuity, creativity, artistic sense, and high-level technology. However, an examination of instances has shown that this system has been applied only to large scale constructions worth 30 billion won or more, and not to small and medium constructions worth less than 30 billion won. This is because the evaluation criteria of the system is structured in a manner that makes it difficult to apply to small and medium constructions. This study presented methods to improve the evaluation factors, categories, and scoring scheme of the technical proposal creation system for medium to small scale constructions to be able to further apply the current system to small and medium constructions. The technical proposal evaluation criteria presented in this study was significant in that it enabled the technical proposal creation system that was mainly used for large scale constructions to be further applied to medium to small scale constructions. And it also contributes to the establishment and settlement of a new technical proposal tendering for medium to small scale constructions.

• Journal of Digital Convergence
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• v.18 no.11
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• pp.195-205
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• 2020

As the use of the Internet has become more common worldwide, digital marketing has gained a great weight as an advertising medium. Low cost and effective digital marketing tools include websites, eyes, blogs, search advertisements, online banners, mailings, YouTube, etc., which most companies around the world use one or more of them for digital marketing. I have. In this environment, global companies with more than one overseas subsidiary have become very interested in how to establish and implement digital marketing strategies. However, many studies have been conducted on the necessity of digital marketing and its implementation strategy, but there are no studies on specific implementation methods that can be applied in practice. Therefore, in this study, we intend to define digital marketing suitable for global SMEs, establish a promotion strategy model, and present a specific implementation strategy establishment planl. Through this, we intend to contribute to establishing a general-purpose SME global digital marketing strategy.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.21-27
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• 2020

Somatic cell nuclear transfer derived embryonic stem cells (NT-ESCs) have significant advantages in various fields such as genetics, embryology, stem cell science, and regenerative medicine. However, the poor establishment of NT-ESCs hinders various research. Here, we applied fasudil, a Rho-associated kinase (ROCK) inhibitor, to develop somatic cell nuclear transfer (SCNT) embryos and establish NT-ESCs. In the study, MII oocytes were isolated from female B6D2F1 mice and performed SCNT with mouse embryonic fibroblasts (MEFs). The reconstructed NT-oocytes were activated artificially, and cultured to blastocysts in KSOM supplemented with 10 μM fasudil. Further, the blastocysts were seeded on inactivated MEFs in embryonic stem cell medium supplemented with 10 μM fasudil. A total of 26% of embryos formed into blastocysts in the fasudil treated group, while this ratio was 44% in the fasudil free control group. On the other hand, 30% of blastocysts were established NT-ESCs after exposure of fasudil, which was significantly higher than the control group (10%). The results suggest that fasudil reduced blastocyst development after SCNT due to inhibition of 2 cell cleavage while improved the establishment of NT-ESCs through the anti-apoptotic pathway.