• Title/Summary/Keyword: meat protein

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Skeletal Ryanodine Receptor 1-Heterozygous PSE (Pale, Soft and Exudative) Meat Contains a Higher Concentration of Myoglobin than Genetically Normal PSE Meat in Pigs

  • Obi, T.;Matsumoto, M.;Miyazaki, K.;Kitsutaka, K.;Tamaki, M.;Takase, K.;Miyamoto, A.;Oka, T.;Kawamoto, Y.;Nakada, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.9
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    • pp.1244-1249
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    • 2010
  • Comparisons of properties between skeletal ryanodine receptor 1 (sRyR1)-heterozygous-mutated and normal types of meat were carried out in pigs using PSE (pale, soft and exudative) meat found during the butchering process. All samples considered to be PSE meat showed irregular running and disorder of the muscle fibers and a wider inter-fiber space upon light microscopic observation. Electron microscopy revealed disintegration, twisting, and disorder of the myofibril arrangement and elimination of the Z line in PSE meat, compared with normal meat. Meat property tests demonstrated greater decreases in water holding capacity, moisture and sarcoplasmic protein, and higher $L^*$ values for the meat color index in PSE meat than in normal meat, but there were no differences in these factors between genetically normal and sRyR1-heterozygous PSE meat. On the other hand, higher $a^*$ and $b^*$ values were observed in sRyR1-heterozygous than in normal PSE meat, and similar alterations to the a* value were observed in terms of the amount of myoglobin and density of the 17-kDa protein band, corresponding to the molecular mass of myoglobin, on SDS-PAGE gels. These results suggest that sRyR1-heterozygous PSE pork contains much more myoglobin than genetically normal PSE meat.

Effects of Dietary Lamb and Beef Meat on the Growth and Protein Utilization in Rats (양고기와 쇠고기의 식이가 흰쥐의 성장과 단백질 이용성에 미치는 영향)

  • 박선희
    • Journal of Nutrition and Health
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    • v.24 no.1
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    • pp.20-29
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    • 1991
  • This study was carried out to compare the protein quality of lamb and beef meat. by feeding to growing rats. Sixty weanling rats, 30 males and 30 females, were blocked into 12 groups(6 gruops of males and 6 groups of females). They were fed casein. beef, or lamb as a protein source at two levels, 6 and 15%, for 5 weeks. The amount of food intake. food efficiency ratio, protein efficiency ratio. body weight gain. and the weights of skeletal muscles and liver were measured. Nitrogen retention, protein content in the liver and skeletal muscles, and the levels of protein and cholesterol in the serum were also assayed. Summarzing the results, there were no significant differences between lamb and beef on the growth and nitrogen utilization in the rats fed same percentage of protein diet. However. rats fed 15% protein diet showed significantly higher growth rate than those fed 6%. Therefore, it can be concluded that lamb is as good a protein food as beef in terms of protein quality.

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The Nutritional Analysis in Fish/Meat/Doenjang and Wild Herbs/Fish/Meat Doenjang (어육장과 산채어육된장의 영양성분 분석)

  • An, Eun-Ju;Park, Won-Jeong;Lee, Je-Hyuk;Kim, MyungHee
    • The Korean Journal of Food And Nutrition
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    • v.29 no.2
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    • pp.171-177
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    • 2016
  • The objectives of this study were to nutritionally evaluate Korean traditional fermentation food, Doenjang, and compare the nutrients in fish/meat-Doenjang and herb/fish/meat-Doenjang. In Doenjang, sugar, protein, fat, dietary fiber, and minerals, such as sodium and calcium, were analyzed using HPLC and the ICP analysis method authorized by the Korean Food Standards Codex. Doenjang is known to prevent arteriosclerosis, cancer and hypertension, and to aid liver function, as well as serving as a protein source. Doenjang, fish/meat Doenjang, and wild-herb/fish/meat Doenjang were made according to the original recipes in antique cookbooks. The highest levels of calories, carbohydrates, and sugar were contained in wild-herb/fish/meat Doenjang. Carbohydrate in the fish/meat Doenjang was higher than in ordinary Doenjang, and ordinary Doenjang had the lowest amount of sugar. Fat was shown to be the most abundant in fish/meat Doenjang, and the least abundant in ordinary Doenjang. Fish/meat Doenjang contained the most Na content, while wild-herb/fish/meat Doenjang had the lowest Na content. In terms of dietary fiber, ordinary Doenjang contained the most, while a wild-herb/fish/meat Doenjang contained the least. Ca content was shown to be most abundant in Doenjang, and the least abundant in fish/meat Doenjang.

Estimation of Ruminal Degradation and Intestinal Availability of Crude Protein in the Animal-Origin Feedstuffs Using Mobile Nylon Bag Technique

  • Lee, S.C.;Moon, Y.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.2
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    • pp.210-214
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    • 1997
  • Ruminal degradation characteristics and intestinal availability of crude protein (CP) in four animal-origin feeds (fish meal, meat meal, viscera meal, feather meal) were estimated by mobile nylon bag technique. Three ruminally and duodenally cannulated Holstein dairy cows (average body wt. 550kg) fed a diet containing 40% concentrate and 60% orchard grass hay on a dry matter (DM) basis. Assuming that the outflow rate of diet in rumen is 5% per hour (k =0.05), contents of quickly degradable CP (QDP), slowly degradable CP (SDP), and undegradable CP (UDP) in the rumen were 27.6%, 9.4%, 63.0% for fish meal, 34.3% 28.1%, 37,6% for meat meal, 43.9%, 12.5%, 43.6% for viscera meal, and 14.4%, 15.8%, 69.8% for feather meal, respectively. Intestinal CP degradability was 51.0% for fish meal, 27.2% for meat meal, 37.9% for viscera meal and 56.2% for feather meal. Available UDP in the intestinal tract was contained 288 g, 217 g, 246 g and 423 g per kilogram DM of diet in fish meal, meat meal, viscera meal and feather meal, respectively.

The Effects of High Pressure and Various Binders on the Physico-chemical Properties of Restructured Pork Meat

  • Hong, Geun-Pyo;Park, Sung-Hee;Kim, Jee-Yeon;Min, Sang-Gi
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.10
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    • pp.1484-1489
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    • 2006
  • This study was carried out to investigate the effect of high pressure and the addition of non-meat proteins on the physico-chemical and binding properties of restructured pork. Pressurizations were carried out at up to 200 MPa and non-meat proteins used as a binder were isolated soy protein (ISP), sodium caseinate (SC), whey protein concentrate (WPC) and egg white powder (EWP). The pH values of all treatments were affected by the level of pressure. $L^*$-value of all treatments increased significantly (p<0.05), while both $a^*$-value and $b^*$-value of all treatments showed a significant decrease (p<0.05) with increasing pressure level. Binders could contribute only additive effects on both pH and color of the treatments. It was found that high pressure improved the water binding capacities and binding strength of the treatments. Binders also improved the binding strength of restructured pork. However, SC and WPC had no effect on water binding properties under high pressure. These results indicate that the application of high pressure had more significant effect on restructuring meat than binders.

Nutritional Improvement of Masoor(Lens esculenta) by Supplementation with Different Kinds of Meat

  • Nighat Bhatty;Nagra, Saeed-Ahmad
    • Nutritional Sciences
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    • v.3 no.2
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    • pp.66-70
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    • 2000
  • The study was conducted to determine the nutiritional value of Masoor (Lens esculenta) in raw and cooked forms. Supplement value of various types of meat i.e. poultry, mutton and beef at 10, 15, and 20 percent levels for dite containing cooked Masoor was also assessed. Nutritional value of Masoor was determined by chemical analysis as well as through rat assay. Masoor contained an average of 23.18 percent protein and less than two percent fibre. Conventional method of cooking resulted in about 2 per cent increase in Masoor protein. Masoor had 0.83 percent of lysine and cooking destroyed 18 percent of it. Other amino acids in Masoor also showed losses on cooking. Protein efficiency ratio (PER) of diets containing raw Masoor was 1.49 and was reduced to 1.44 by cooking. Cooking of Masoor did not alter true digestibility (TD) percentage. However, net protein utilization (NPU) was improved from 44.60 in raw to 47.77 in cooked. Diets containing cooked Masoor and supplemented with different types of meat significantly improved PER (1.45 to 1.65), TD 76.03 to 87.84 percent and NPU 42.84 to 50.72 percent over non supplemented diets. 20 percent level of supplemented meat showed comparatively better results than other levels in case of improvement in PER, TD and NPU.

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Effects of Meat Meal, Blood Meal or soybean Meal as a Dietary Protein Source Replacing Fish Meal in Parrot Fish, Oplegnathus fasciatus (돌돔사료의 대체 단백질원으로서 육분, 혈분 및 대두박의 효과)

  • 강용진;이상민;양상근;배승철
    • Journal of Aquaculture
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    • v.12 no.3
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    • pp.205-212
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    • 1999
  • A feeding trial was conducted to evaluate dietary protein sources replacing fish meal for parrot fish, Oplegnathus fasciatus. A control diet with white fish meal as a protein source was included. White fish meal in the isonitrogenous diet was replaced with each of 22% meat meal, 17% blood meal, or 31% soybean meal. Triplicate groups of 25 fish initially averaging 26 g were fed four experimental diets for 7 weeks in a flow-through tank system. Weight agin and feed efficiency in fish fed diet containing 17% blood meal (this diet also contained 45% white fish meal) were not significantly different from those in fish fed the control diet (P>0.05). Fish fed diet containing 22% meat meal had lower weight gain and feel efficiency than those of fish fed the control diet (P<0.05). weight gain of fish fed diet containing 31% soybean meal was not different from that of fish fed the control diet, but feed efficiency of fish fed the diet was lower than that of fish fed the control diet.

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Evaluation of antioxidant property of heat shock protein 90 from duck muscle

  • Zhang, Muhan;Wang, Daoying;Xu, Xinglian;Xu, Weimin
    • Animal Bioscience
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    • v.34 no.4
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    • pp.724-733
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    • 2021
  • Objective: The objectives of this study were to investigate the direct antioxidative effect of 90 Kda heat shock protein (Hsp90) obtained from duck muscle. Methods: The interaction of Hsp90 with phospholipids and oxidized phospholipids was studied with surface plasmon resonance (SPR), and their further oxidation in the presence of Hsp90 was evaluated with thiobarbituric acid reactive substances (TBARS) assay. The scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) was measured, and the electron paramagnetic resonance (EPR) spectroscopy in combination with 5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide and 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) was utilized to determine the abilities of Hsp90 in scavenging hydroxyl and PTIO radicals. Results: SPR showed Hsp90 could bind with both phospholipids and oxidized phospholipids, and prevent their further oxidation by the TBARS assay. The DPPH and ABTS scavenging activity increased with Hsp90 concentration, and could reach 27% and 20% respectively at the protein concentration of 50 μM. The EPR spectra demonstrated Hsp90 could directly scavenge ·OH and PTIO· radicals. Conclusion: This suggests that Hsp90, a natural antioxidant in meat, may play an important role in cellular defense against oxidative stress, and may have potential use in meat products.

Effects of Wheat Fiber on the Quality of Meat Batter (Wheat Fiber 첨가가 Meat Batter의 품질에 미치는 영향)

  • Choi, Yun-Sang;Lee, Mi-Ai;Jeong, Jong-Youn;Choi, Ji-Hun;Han, Doo-Jeong;Kim, Hack-Youn;Lee, Eui-Soo;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.27 no.1
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    • pp.22-28
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    • 2007
  • The purpose of this study was to investigate the effect of wheat fiber as a replacement for isolated soy protein (ISP) or concentrated soy protein (CSP) on the quality characteristics of meat batter. Meat batter formulations were prepared with 2% levels of ISP, CSP, or wheat fiber. The pH values of each formulation were not significantly different (p>0.05). The L-values of meat batter containing wheat fiber, ISP, or CSP were higher than the control, however the a- and b-values were lower than the control (p<0.05). Relative to the control meat batter, the water holding capacity, viscosity, hardness, gumminess, and chewiness of meat batter containing ISP, CSP, or wheat fiber was significantly higher than the control (p<0.05). All supplemented meat batters had significantly lower cooking loss and emulsification stability than the control (p<0.05), with no significant difference among the supplemented meat batters. The results of this study show that the use of wheat fiber as an ISP or CSP replacer does not affect the overall quality of meat batter.