• 제목/요약/키워드: mealworm (Tenebrio molitor)

검색결과 70건 처리시간 0.032초

치어기 넙치(Paralichthys olivaceus)의 저어분 사료 내 어분 대체원으로써 동애등에(Hermetia illucens)와 갈색거저리(Tenebrio molitor) 이용성 평가 (Evaluation of Black Soldier Fly Hermetia illucens and Mealworm Tenebrio molitor as a Fish Meal Substitute in a Low-Fish Meal Diet for Juvenile Olive Flounder Paralichthys olivaceus)

  • 송상현;임현운;이경준
    • 한국수산과학회지
    • /
    • 제56권6호
    • /
    • pp.861-869
    • /
    • 2023

  • This study aimed to evaluate the effectivity of full-fat black soldier fly Hermetia illucens (BSF) and defatted mealworm Tenebrio molitor (MW) larvae meal as a fish meal (FM) substitute in a low-fish meal (LFM) diet for juvenile olive flounder Paralichthys olivaceus. The LFM diet comprising 45% FM with tankage meal, poultry byproduct meal, soy protein concentrate and wheat gluten was the control diet. Three experimental diets were 10% FM in Con with BSF, MW and a mixture of both at the same ratio (designated as B10, M10 and B5M5, respectively). Four hundred and forty-fourth juvenile P. olivaceus (34.3±0.1 g) were randomly distributed into 12 tanks (425 L) in three replicate groups per treatment and fed the experimental diets for 15 weeks. At the end of the feeding trial, growth performance, survival, biological indices (condition factor, viscerosomatic index, hepatosomatic index), non-specific immune responses (lysozyme, myeloperoxidase) and intestinal histology (villi length and goblet cells) were not significantly affected by treatments. Feed utilization was significantly decreased in M10 compared to the control group. Alanine aminotransferase level was significantly higher in M10 than in the control group. Glucose level was significantly lower in B10 than in the control group. These results suggest that BSF and MW can be used as FM substitutes. However, considering feed conversion ratio and AST level, MW availability is thought to be lower than that of BSF, and feeding fish with a diet containing MW for an extended period is thought to adversely affect fish growth.

  • https://doi.org/10.5657/KFAS.2023.0861 인용 PDF HTML

SH-SY5Y 인간 신경모세포종 세포에서 MPTP 유발 세포 독성에 대한 거저리(Tenebrio molitor) 추출물의 보호효과 (Protective effects of mealworm (Tenebrio molitor) extract on N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced cellular toxicity in SH-SY5Y neuroblastoma cells)

  • 조인호;김유지;김선태
    • 대한임상독성학회지
    • /
    • 제21권2호
    • /
    • pp.81-91
    • /
    • 2023

  • Purpose: Edible insect extracts have been used as an alternative source for medicinal supplements due to their significant antioxidative and anti-inflammatory activity. Recent studies have reported that anti-microbial peptides from insects have neuroprotective effects on dopamine toxins. The purpose of this study was to investigate the protective functions of mealworm (Tenebrio molitor) extract (MWE) on N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced cellular toxicity in SH-SY5Y neuroblastoma cells. Methods: Cellular toxicity induced by the MPTP toxin and the impact of MWE on cell survival were analyzed using MTT assays. DAPI staining was performed to observe apoptotic phenomena caused by MPTP. Changes in caspase-3 activity and protein expression were observed using enzyme activity assays and western blot assays, respectively. Results: MWE exerted significant antioxidant activity, which was measured by both DPPH and ABTS radical assays, with a dose-dependent relationship. Furthermore, MWE resulted in cellular proliferation in SHSY5Y cells in a dose-dependent manner. Furthermore, MWE pretreatment significantly inhibited MPTP-induced cytotoxicity, with a dose-dependent relationship. The morphological characteristics of apoptosis and increased reactive oxygen species induced by MPTP were also significantly reduced by MWE pretreatment. Conclusion: MWE treatment significantly attenuated MPTP-induced changes in the levels of proteins associated with apoptosis, such as caspase-3 and PARP. These findings suggest that MWE exerts neuroprotective effects on human neuroblastoma SH-SY5Y cells subject to MPTP-induced dopaminergic neurodegeneration.

  • https://doi.org/10.22537/jksct.2023.00021 인용 PDF