• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.69-74
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• 2000

The medium for erythritol production by Torula sp. in a 500-ml baffled flask was optimized to be 300 g/I sucrose, 10 g/I yeast extract, 3 g/I $KH_2PO_4$, and 10 mg/I $CuSO_4{\cdot}5H_2O{\;}at{\;}34^{\circ}C$ with initial pH of 5.5. Using this optimal medium, erythritol of 166 g/I was obtained after 140 h of cultivation, corresponding to 55.3% of the erythritol yield from sucrose with a productivity of 1.11 g/I/h. Optimal concentrations of carbbon and nitrogen sources in a fermentor were higher than that in a flask due to the higher oxygen supply of the fermentor. Employing the medium containing 300 g/I or 400 g/I sucrose for the determination of optimal C/N ratio, the C/N ratio was found to be more important than the nitrogen concentration for effective erythritol production, The optimal ratio of yeast extract to sucrose (g/g) was 20. The yield and productivity of erythritol were maximal in the medium containing 400 g/I sucrose and 20 g/I yeast extract. when dissolved oxygen in the culture was increased, the cell mass increased but the erythritol production was manimal in the range of 5 to 10% of dissolved oxygen. Under the optimal the rane of 5 to 10% of dissolved oxygen. Under the optimal culture condition of the fermentor, a final erythritol concentration of 200 gI was obtained after 120 h with a yield of 50% and the productivity was 1.67 g/I/h. The yield was the highest among erythritol-producting microorganisms

• Ocean and Polar Research
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• 제25권1호
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• pp.31-40
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• 2003

In August-September 2001, 15 samples of bottom sediments were collected in the inner, middle and open parts of Amursky Bay near Vladivostok, Russia, and barfin plaice Pleuronectes pinnifasciatus was sampled from the inner and the middle locations of the bay. In the sediments from all three sites elevated concentrations of several heavy metals, i.e. Zn ($102-115{\mu}/g$ dry weight), Ni $(70-73{\mu}g/g)$ and Cu $(27-35{\mu}g/g)$ were discovered. The contents of oil hydrocarbons were very close to or slightly higher than the maximal normal environmental background level, $100{\mu}g/g$ dry weight. The sediments contained negligible amounts of hexachlorocyclohexane, while DDT concentrations were quite high (1.7-16.3ng/g dry weight). Generally, there were no substantial differences in the pollution levels of the locations studied and our results resembled those reported for Amursky Bay in the 1990s. Surprisingly, in 2001 'fiesh' DDT comprised 70-85% of the total DDT content in sediment from all the locations studied. In fish liver total DDTs concentrations were 212.8 and 122.54 ng/g wet weight for the inner and the middle locations, respectively, and 'fresh' DDT comprised 35 and 64% of DDTs, respectively. These results provide evidence of recent input of DDT from an unknown source into the ecosystem of Amursky Bay. Histopathological changes revealed in the plaice liver (vacuolization of hepatocytes, coagulative necrosis of hepatocytes, inflammatory reaction, and necrosis of epithelial cells of bile ducts) are probably connected with an intensive metabolism of DDT in the fish organism. No histological and histomorphometric differences were found in the state of the interrenal tissue. Similar condition of the liver and the interrenal tissue in barfin plaice sampled from the inner and the middle locations of Amursky Bay may be explained by the absence of great differences in the pollution levels of these sites.

• 한국미생물·생명공학회지
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• 제17권6호
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• pp.545-551
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• 1989

알칼리성 Bacillus속 8-16 균주의 내열ㆍ알칼리성단백질 분해효소를 정제하여 그 특성을 조사하였다. 본 균주의 알칼리성 protease를 아세톤침전, CM-셀룰로즈크로마토그래피, Sephadex G-100 및 G-75 젤 여과법으로 정제하였고 비활성이 37배되게 하여 단일단백질이 될 때까지 순수정제하였다. 이 효소는 7$0^{\circ}C$ pH 11에서 pH 12 사이에서 최대 활성을 나타냈고 6$0^{\circ}C$에서는 한시간 동안 안정하였다. 이 효소의 $K_m$치는 1.3mg/$m\ell$이며 분자량 33,000으로 추정된다. 또한 이 효소는 Cu$^{2+}$ 및 Mn$^{2+}$에 의해서 약간 활성화되고 Ag$^+$, Hg$^{2+}$ 및 PMSF에 의해서 저해되므로 활성부위에 serine기가 관여하는 것으로 추정된다.

• 약학회지
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• 제54권4호
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• pp.226-231
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• 2010

Oral fluid has become increasingly popular as an alternative specimen in the field of driving under the influence of drugs (DUID) and work place drug testing. In this study, an analytical method for the detection and quantification of ${\Delta}^9$-tetrahydrocannabinol (THC) and its metabolite, 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THC-COOH) in oral fluid by SPE and GC-MS was established and fully validated. The stability of THC and THC-COOH in oral fluid during storage was also determined by examining the THC and THC-COOH concentration changes depending on time and container materials. Oral fluid samples were kept over 21 days at room temperature, $-4^{\circ}C$ and $-20^{\circ}C$ in two different specimen collection tubes; glass and polypropylene tubes. Three replicates for each condition with different temperature and types of a container were analyzed at five different time points over 21 days. When oral fluid samples were stored in glass tubes, the loss of both THC and THC-COOH was less than 10% at all room temperature, $-4^{\circ}C$ and $-20^{\circ}C$. However, in polypropylene tubes, the loss of both THC and THC-COOH increased significantly over the study period. In particular, the concentration of THC decreased more rapidly than that of THC-COOH at room temperature and the maximal percentage of THC lost was 90.3% after 21 days. The result indicates that it would be necessary to collect oral fluid samples in glass containers and cool the samples until analysis in order to prevent the degradation of analytes.

• 수산해양교육연구
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• 제27권1호
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• pp.63-73
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• 2015

The purpose of this study was to examine EMG activities and VMO/VL ratio of the vastus medialis oblique, and vastus lateralis during step up exercise according to ankle and knee positions in soccer players with patellofemoral pain syndrome. Methods: Subject(patellofemoral pain syndrome, PFPS: n=8 and without PFPS, non PFPS; NPFPS: n=8) perfomed step up exercise at each knee and ankle position(knee flexion $30^{\circ}$, $60^{\circ}$, and $90^{\circ}$, ankle internal rotation $30^{\circ}$, neutral, and external rotation $30^{\circ}$) while EMG activity was collected. The EMG signals were expressed by the % maximal voluntary isometric Contraction(%MVIC) values. Statistical analysis consisted of two way repeated measures analysis of variance with post hoc analysis. Results: Main results were as follows: 1) EMG of VMO, and VL was tend to be lower in PFPS compared to NPFPS. 2) EMG of VMO and VL with knee flexrion $60^{\circ}$ was significantly higher the results with knee flexion $30^{\circ}$, and $90^{\circ}$. VMO and VL with ankle external rotation $30^{\circ}$ was significantly higher the results with internal rotation $30^{\circ}$ and neutral position. Conclusion: Considering the EMG activity was reduced due to the to the PFPS and that performing step up with knee flexion $60^{\circ}$ with ankle external rotation $30^{\circ}$ position may provide the most effective condition for patients with patellofemoral pain syndrome.

• Journal of Oral Medicine and Pain
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• 제20권2호
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• pp.489-496
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• 1995

The purpose of this study was to evaluate an effect of change on head posture initial occlusal contacts with measuring the distances between initial occlusal contacts and maximum intercuspal position at different head posture in TMDs patient. For this study, 24 patients from age 13 to 36 were selected, they were examined health history taken, patients who have sign and symptoms of TMDs were examine before the study. For the normal group, 21 adults from age 23 to 25 were selected. They have normal or class I molar relationship, and have no other prosthetic restorations. Difference on distance between initial occlusal contact and maximum intercuspal position with mandibular kinesiograph$(MKG^R)$(K6 diagnostic system, Myo-tronic Inc, USA) in upright, supine, 45$^{\circ}$ extension, 30$^{\circ}$ flexion position of the head were measured. The Frankfort horizontal plane was used as a reference plane. The results were as follows : 1. There were significant differences between initial occlusal contacts of the normal and patient group on upright position and 30$^{\circ}$ flexion of the head(p<0.05, p<0.01) 2. The position of the initial occlusal contacts have a tendency to place anterior and inferior to maximal intercuspal position in upright position and 30( flexion of the head as well as posterior and inferior in supine position and 45$^{\circ}$ extension of the head in the normal and patient groups. 3. There were significant differences among the initial occlusal contacts between uptight and supine position; upright and 45$^{\circ}$ extension of the head(p<0.05); supine position and 30$^{\circ}$ flexion of the head, .and 30(flexion and 45$^{\circ}$ extension of the head in the patient group(p<0.01) The result have shown that after treatment on the supine position, it may be necessary to check occlusal contact on the upright position as well ass flexion of the head. It may need careful adjustment in occlusal condition on upright position of TMDs patient.

• The Journal of Advanced Prosthodontics
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• 제9권1호
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• pp.45-51
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• 2017

PURPOSE. This study aims to investigate the effects of four different lateral occlusion schemes and different excursions on peri-implant strains of a maxillary canine implant. MATERIALS AND METHODS. Four metal crowns with different occlusion schemes were attached to an implant in the maxillary canine region of a resin model. The included schemes were canine-guided (CG) occlusion, group function (GF) occlusion, long centric (LC) occlusion, and implant-protected (IP) occlusion. Each crown was loaded in three sites that correspond to maximal intercuspation (MI), 1 mm excursion, and 2 mm excursion. A load of 140 N was applied on each site and was repeated 10 times. The peri-implant strain was recorded by a rosette strain gauge that was attached on the resin model buccal to the implant. For each loading condition, the maximum shear strain value was calculated. RESULTS. The different schemes and excursive positions had impact on the peri-implant strains. At MI and 1 mm positions, the GF had the least strains, followed by IP, CG, and LC. At 2 mm, the least strains were associated with GF, followed by CG, LC, and IP. However, regardless of the occlusion scheme, as the excursion increases, a linear increase of peri-implant strains was detected. CONCLUSION. The peri-implant strain is susceptible to occlusal factors. The eccentric location appears to be more influential on peri-implant strains than the occlusion scheme. Therefore, adopting an occlusion scheme that can reduce the occurrence of occlusal contacts laterally may be beneficial in reducing peri-implant strains.

• 식물조직배양학회지
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• 제26권1호
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• pp.7-14
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• 1999

형질전환된 소리쟁이 모상근의 기내 배양시 그 생장 특성과 anthraquinone생산성을 조사하기 위하여 소리쟁이의 모상근을 Agrobacterium rhirogenes strain $A_4$ 접종에 의하여 잎 절편으로부터 유기하여 부정근과 비교하였다. 잎 절편에서의 부정근 형성은 5 $\mu$M NAA를 첨가한 MS배지에서 가장 양호하였다. Paper electrophoresis를 통한 opine분석 결과 모상근에서만 mannopine이 검출되고 부정근에서는 검출되지 않았다. 부정근과 모상근 모두에서 2차근인 측근 근단은 호르몬을 첨가하지 않은 배지에서 신장만 할 뿐 분지하지 않았으나, 1차근인 주근의 근단은 많은 측지를 내며 빠르게 생장하였다. Anthraquinone 함량 증대에 따른 부정근과 모상근의 생장에는 MS기본 배지가 가장 양호하였다. 부정근과 모상근 배양시 생장량과 anthraquinone함량은 5% sucrose를 포함한 0.05 $\mu$M NAA와 0.1 $\mu$M kinetin으로 조성된 MS 액체 배지에서 가장 높은 것으로 나타났으며, 모상근의 anthraquinone함량은 배양 일수에 따라 증가하다가 배양 25일 이후에는 감소하였다.

• 대한전자공학회논문지TC
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• 제41권6호
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• pp.1-7
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• 2004

직접 변환 방식 수신기법은 SDR기반 미래 광대역 또는 다중 대역 무선통신 시스템을 위한 중요한 해의 하나로 인식되고 있다. 본 논문에서는 광대역의 대역폭을 가지면서도 시스템의 유연성을 극대화할 수 있도록 SDR기반 직접 변환 수신기에 적용가능한 I 및 Q 신호의 생성에 관하여 연구하였다. 먼저 실제의 SDR 기반 통신 환경을 고려한 직접 변환 SDR 시스템을 모델링하고, 수신기에서의 위상 오류의 영향을 분석하며 이에 따른 I/Q 채널의 준최적 재생 알고리즘을 제안하였다. 제안된 알고리즘은 실시간 early-late compensator 구조를 통하여 송신단과 수신단의 위상 오류를 실시간으로 보정하고 랜덤한 채널 잡음환경에서도 보다 안정된 성능을 유지하게 한다. 컴퓨터 시뮬레이션을 통하여, $45{\~}55$도의 랜덤 위상 오류가 난 경우, 제안된 시스템은 기존 시스템과 비교하여 약 4dB이상의 성능 개선이 있음을 확인하였다.

• 대한물리의학회지
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• 제11권4호
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• pp.11-17
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• 2016

PURPOSE: There are several standard interventions for managing Achilles tendinitis, including eccentric exercise and calf muscle stretches, orthoses, electrotherapy, and taping. However, no study has determined the effect of non-elastic taping on deloading the Achilles tendon while vertical jumping. Therefore, this study determined the effect of non-elastic taping on ankle dorsiflexion and the triceps surae muscle activity while vertical jumping in healthy subjects. METHODS: The study recruited 17 participants. A motion analysis system was used to measure the angle of ankle dorsiflexion and wireless surface electromyography was used to measure the soleus and gastrocnemius activities while vertical jumping. Non-elastic taping was applied on randomized leg side. All subjects performed maximal effort vertical jumps without and with non-elastic taping, with three trials for each condition. The mean peak dorsiflexion and muscle activities during the three trials were calculated and paired t-tests were used to compare the mean values without and with non-elastic taping. Significance was defined as (p<.05). RESULTS: The maximum angle of ankle dorsiflexion and activity of the gastrocnemius muscle decreased significantly when non-elastic tape was applied (p<.05), while there was no significant difference in the soleus activity between no-taping and taping (p>.05). CONCLUSION: We introduce non-elastic taping as a method to decrease maximum ankle dorsiflexion and gastrocnemius activity while vertical jumping.