• Journal of Korean Academy of Nursing
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• v.16 no.3
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• pp.78-96
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• 1986

The sympathico-adrenergic system and the hypophyseal-adrenocortical system mediates the regulation of the internal milieu. And the kidneys regulate both water and electrolyte balance of the body fluid. The kidneys are the sites of production of renin which participate indirectly in maintaining renin. angiotensin-aldosterone system. These system de-serve special attention in the context of adjustment the effects on the body function. And so, maximal exercise and work load are associated with home-osthetic function. The nurses working in the hospital have been complained of fatigue and stress by frequent duty changes and overload. In order to define this, the possible changes of hormonal excretion during three-shift nursing practice were investigated. Urine samples were collected at pre-duty and post-duty, and were measured with chemical assay and radioimmunoassay in 30 nursing students, in nursing practice and 43 nursing students, in studying. The results obtained were as follows. 1. In nursing practice, urinary norepinephrine concentration showed a marked increase during day duty, urinary cortisol concentration showed a marked increase during evening duty, and urinary renin concentration was increased in night duty, 2. Corrected ratio of urinary sodium excreted by the urinary excretion of creatinin (UNa/UCr) and UCl/UCr showed a marked decrease during night duty. Nursing practice did not affect on the UK/UCr and urinary concentrating ability. From these results, it is suggested that further studies the define the effects on some physiological function of the three-shift nursing practice against circadian rhythm are needed for better working condition of nurses.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.286-297
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• 1994

The optimum nutritional and cultural conditions of polygalacturonase by Ganoderma lucidum in liquid culture were studied. The optimal temperature, pH, and the duration of culture for production of the enzyme was $30^{\circ}C$, 5.5 and 14 days, respectively. The maximal production of the enzyme was obtained in a synthetic medium containing 10 g of pectin, 10 g of soluble starch, 1 g of yeast extract, 2 g of peptone, 1 g of phenylalanine, 2 g of $KH_2PO_4$, 0.2 g of $MgSO_4{\cdot}7H_2O$, 0.05 g of $CaCI_2$ and 100 g of $thiamin{\cdot}HCI$ in 1000 ml of distilled water.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.1
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• pp.75-81
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• 1997

This study was wnducte to investigate the effects of treatment level and seasons of sluny hm bovine feces on the productivity of rye, N efficiency and improvement of soil fertility under the Korean climate condition. The results obtained fiom this study summarized as follows ; 1. The highest dry matter yield of rye was obtained in the partial fertilization of sluny in spring or autumn. There is no differences of dry matter yield between spring and autumn application. 2. With increasing the amount of slurry-N, the dry matter yield of rye was signigicantly increased up to 100Kg sluny Nha. As the level of slurry-N rises above about 100Kg N/ha, the maximal yield of dry matter was unchanged or declined. 3. As the level of sluny fertilization rises, the crude protein content of rye increases significantly. However the contents of crude protein was less affected by the application seasons. 4. The amount of nitrogen which produced 6om rye is dependent upon the level of slurry-N. The highest nitrogen yield of rye was obtained by the partial fertilization of sluny-N. 5. The season or amount of slurry treatments did not affect the organic matter content in soil. N-content in soil was the lowest by the partial fertilization of slurry in spring or autumn. However, N-content was increased with the higher level of sluny-N.

• Journal of environmental and Sanitary engineering
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• v.12 no.2
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• pp.1-12
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• 1997

This research aims to develop biofilm process for the nutrient removal of piggery wastewater. The developed process is the four stage anoxic-oxic biofilm process with recirculation of the final effluent. In summery, the results are as follows: 1. Nitrification in the piggery wastewater built up nitrite because of the high strength ammonia nitrogen. The nitrification of nitrobacter by free ammonia was inhibited in the total ammonia nitrogen loading rate with more than 0.2 kgNH$_{3}$-N/m$^{3}$·d. 2. The maximal total ammonia nitrogen removal rate was obtained at 22$\circ $C and without being affected by the loading rate. But total oxidized nitrogen production rate was largely affected by loading rate. 3. Autooxidation by the organic limit was a cause of the phosphorus release in the aerobic biofilm process. But the phosphorus removal rate was 90 percent less than the influent phosphorus volumetric loading rate of above 0.1 kgP/m$^{3}$·d. Therefore, the phosphorus removal necessarily accompanied the influent loading rate. 4. On the anoxic-oxic BF process, the total average COD mass balance was approximately 67.6 percent. Under this condition, the COD mass removal showed that the cell synthesis and metabolism in aerobic reactor was 42.8 percent and that the denitrification in anoxic reactor was 10.7 percent, respectively.

• The Korean Journal of Food And Nutrition
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• v.12 no.3
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• pp.271-278
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• 1999

We isolated activnmycetes LAM-98-80 as strain producing an effective antibiotic for cephalosporin re-sistant pathogenic PSeudomonas sp. and identified as Streptomyces sp. LAM-98-80 from cultural and phyisological characteristics. We investigated the optimal culture conditions for producation of an anti-biotic becoming effective for cephalsporin-resistant pathogenic Pseudomonas sp. It was found that 1.5% soluble starch and 1.0% yeast extract were good as carbon and nitrogen source respectively. The pro-duction of antibiotic was also activated by 0.04% Mn2+ as 80% degree. The optimum initial pH on pro-ductio of antibiotic was pH 7.0. The culture condition for the maximal productivity of the antibiotic was at 3$0^{\circ}C$ for 5 days. The cephalosporin-resistant pathogenic Pseudomonas sp. as test bacteria was rev-ealed to resist antibiotic of cepha families but revealed to not resist those of $\beta$-lactam families ampicil-lin and amoxicillin. Parital purified antibiotic was stable for the pH from 3 to 9 and was also stable when treated at 70 $^{\circ}C$ for 1 hour, This antbiotic was effective against all gram positive and negative bac-teria but was not effective against molds and yeasts.

• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.450-455
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• 1991

A protease hyperproducer, ampicillin resistant mutant, Serratia sp. SMNT-1 was selected from Serratia marcescens ATCC 21074 by mutagenesis. The protease productivity of this strain was about 11 times as much as that of the parental strain. The enzyme showed maximal activity at pH 9.0 and $40^{\circ}C$ and was stable over the pH range from 6.0 to 10.0 at $4^{\circ}C$, whereas it was unstable at $37^{\circ}C$ in alkaline condition. the enzyme was inactivated by heating at $60^{\circ}C$ for 10 min. The enzyme was inactivated by EDTA and reactivated by $Zn^{2+}, Co^{2+},\; and \; Mn^{2+}$, but the proteoiytic activity of the enzyme was not affected by DFP. From the above results, the protease produced by Serratia sp. SMNT-1 was classified as a metalloprotese.

• Food Science of Animal Resources
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• v.18 no.2
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• pp.142-148
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• 1998

This study was conducted to investigate the quality change of beef muscle, which was thawed by different thawing rate in order to utilize it as fundamental data for establishing optimal and thawing condition. Chilled beef round which was purchased at a commercial market was used. The samples were frozen for 30min(time required to pas through the maximal ice forming zone, -1$^{\circ}C$∼-7$^{\circ}C$) and the thawing conditions were 3.9cm/hr, 0.21cm/hr, 0.13cm/hr. Thawing losses of rapidly thawed meat(3.9cm/hr) were significantly(p＜0.05) lower(6.10%), but cooking and total losses were the highest as 48.17% and 56.44%, respectively(p＜0.05). Characteristics such as color, flavor, texture and overall quality at different the thawing rates showed similar scores, but slightly increased after storage for 24hr. Juiciness of rapidly thawed meat was significantly(p＜0.05) higher than that compared to the other thawing rates.

• Physical Therapy Korea
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• v.14 no.2
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• pp.29-36
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• 2007

Muscle tone (stiffness/hardness) or muscle compliance changes during muscle contraction. The purposes of this study were to assess the intrarater and interrater reliabilities of the Myotonometer$^{(R)}$, electronic device that quantifies muscle tone. Two raters used the Myotonometer to assess the right bicep brachia and quadriceps muscles of 30 voluntary persons without any orthopedic or neurological problems (age range, 18~21 yrs). Muscles were measured in a relaxed state and during brief sustained voluntary maximal isometric contraction. Intrarater correlation coefficients were calculated for each muscle and for each condition (relaxed and contracted). Intrarater reliabilities (intraclass correlation coefficients, ICCs) ranged from .778 to .954, relaxed, biceps brachia), .926 to .963 (contracted, biceps brachia), .935 to .990 (relaxed, quadriceps) and .679 to .952(contracted, quadriceps). Interrater reliabilities ranged from .652 to .790 (relaxed, biceps brachii), .813 to .907 (contracted, biceps brachii), .831 to .950 (relaxed, quadriceps) and .849 to .937 (contracted, quadriceps). Myotonometer measurements had high to very high intrarater and interrater reliability for measurements of the biceps brachia and quadriceps muscles.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.49-56
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• 2000

The 2-O-$\alpha$-D-glucopyranosyl L-ascorbic acid (AA-2G) which was enzymatically glucosylated with the cyclodextrin glucanotransferase (CGTase) [EC 2.4.1.19] from Bacillus sp. JK-43 has been reported previously. The presnet experiments examined the optimal conditons for the productio of AA-2G from AA and soluble starch, and characterized the properties of the CGTase from Bacillus sp. JK-43. The reaction mixture for the maximal production of AA-2G was followings; 12% total substrate concentration, 1,400 usits/mL of CGTase and a mixing ratio of 2 : 3(g or AA : g of soluble starch). Under this condition, 1.76mM of AA-2G, which corresponded to 2.53% yield based on AA, was produced after incubation for 24hrs at 45$^{\circ}C$ (pH 5.5). The optimum pH and temperature for the CGTase activity were 6.0 and 45$^{\circ}C$, respectively. The enzyme was stable at pH 5.5 to 9.5, and at temperature up to 5$0^{\circ}C$. The thermostability of the enzyme could be enhanced up to 6$0^{\circ}C$ by the addition of 30mM CaCl2.

• KSBB Journal
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• v.13 no.2
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• pp.203-208
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• 1998

We investigated the effects of dissolved oxygen (D.O.) and fructose (C-source) on cell growth and biosynthesis of cyclosporin A (CyA) produced as a secondary metabolite by a wild-type filamentous fungus, Tolypocladium inflatum. This was performed by controlling the level of D.O. and the residual C-source, as required, through adjustment of medium flow rate, medium concentration and agitation rate in fed-batch cultures. CyA production was furned out to be maximal, when D.O. level was controlled around 10% saturated D.O. and concentration of the C-source was maintained sufficiently low (below 2 g/L) not to cause carbon catabolite repression. Under this culture condition, we obtained the highest values of CyA concentration (507.14 mg/L), Qp (2.11 mg CyA/L/hr), $Y_x/s$ (0.49 g DCW/g fructose), $Y_p/s$<(22.56 mg CyA/g fructose), and YTEX>$_p/x$ (48.31 mg CyA/g DCW), but relatively lower values of cell concentration (11.98 g DCW/L) and cell productivity (0.043 g DCW/L/hr), in comparison with other parallel fed-batch fermentation conditions. These results implied that, in the carbon-limited culture with 10% saturated D.O. level, the producer microorganism utilized the C-source more efficiently for secondary metabolism.