• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.3
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• pp.43-53
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• 2019

It is well-known fact that the stone-mastic asphalt (SMA) mixture has shown superior rut resistance, compared with the dense-graded asphalt (DGA) mixture in highway pavements. However, the SMA is measured to be inferior to DGA mixes when tested by well-known high-temperature test methods, such as the wheel tracking (WT), asphalt pavement analyzer, the Marshall Stability and Kim Test. Therefore, this study examined the reasons why it was measured to be inferior, and devised a potential procedure by which the superiority of SMA could be measured at $60^{\circ}C$. The strength against deformation ($S_D$), which was known to show very high correlation with WT results for DGA mixes, was measured by the Kim Test on the specimen confined in the compacted mold. In standard Kim Test, which used the specimen without confinement, the DGA was measured to show higher $S_D$ than the SMA. But by confining specimen, it was found that the $S_D$ of SMA was measured to be higher than that of DGA. Therefore, the confined static test protocol devised in this study was found to be feasible for evaluating rut resistance of SMA mix.

• Journal of Oral Medicine and Pain
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• v.36 no.3
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• pp.147-160
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• 2011

Eugenol (4-allyl-2-methoxyphenol) is a natural phenolic constituent extensively used in dentistry as a component of zinc oxide eugenol cement and is applied to the mouth environment. Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. This study was undertaken to investigate the synergistic apoptotic effect of co-treatment with a natural product, CGM and natural phenolic compound, eugenol on SCC25 human tongue squamous cell carcinoma cell line. To investigate whether the co-treatment with eugenol and CGM compared to each single treatment efficiently reduces the viability of SCC25 cells, MTT assay was conducted. Induction and augmentation of apoptosis were confirmed by Hoechst staining, TUNEL staining and DNA hypoploidy. Westen blot analysis and immunofluorescent staining were performed to study the alterations of the expression level and the translocation of apoptosis-related proteins in co-treatment. In this study, co-treatment of with eugenol and CGM on SCC25 cells showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the increase and decrease of Bax and Bcl-2, decrease of DNA content, the release of cytochrome c into cytosol, translocation of AIF and DFF40 (CAD) onto nuclei, and activation of caspase-3, caspase-6 caspase-7, caspase-9, PARP, Lamin A/C and DFF45 (ICAD) whereas each single treated SCC25 cells did not show or very slightly these patterns. Although the single treatment of 40 ${\mu}g$/ml CGM and 0.5 mM eugenol for 24 h did not induce apoptosis, the co-treatment of these reagents prominently induced apoptosis. Therefore our data provide the possibility that combination therapy with CGM and eugenol could be considered as a novel therapeutic strategy for human oral squamous cell carcinoma.

• International Journal of Oral Biology
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• v.34 no.2
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• pp.61-72
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• 2009

Chios gum mastic (CGM) is a resin produced from the stem and leaves of Pistiacia lentiscus L var chia, a plant which grows only on Chios Island in Greece. CGM has been used for many centuries as a dietary supplement and folk medicine for stomach and duodenal ulcers in many Mediterranean countries and is known also to induce cell cycle arrest and apoptosis in some cancer cells. In this study, we further investigated the induction and mechanisms underlying the apoptotic response to CGM treatment in the SCC25 human tongue squamous cell carcinoma cell line. The viability of SCC25 cells, human normal keratinocytes (HaCaT cells) and human gingival fibroblasts (HGF-1 cells), and the growth inhibition of SCC25 cells were assessed by MTT assay and clonogenic assay, respectively. Staining with Hoechst and hemacolor dyes and TUNEL assays were employed to detect SCC25 cells undergoing apoptosis. SCC25 cells were treated with CGM, and this was followed by western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses. CGM treatment of SCC25 cells was found to result in a time- and dosedependent decrease in cell viability, a dose-dependent inhibition of cell growth, and apoptotic cell death. Interestingly, CGM showed a remarkable level of cytotoxicity in SCC25 cells but not in normal cells. Tested SCC25 cells also showed several lines of apoptotic manifestation. Taken together, our present findings demonstrate that CGM strongly inhibits cell proliferation by modulating the expression of G1 cell cycle-related proteins and induces apoptosis via the proteasome, mitochondria and caspase cascades in SCC25 cells.

• International Journal of Oral Biology
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• v.41 no.2
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• pp.53-62
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• 2016

In the present study, we evaluated the effect of CGM on osteogenic differentiation of cultured osteoblasts, and determined whether combination treatment with LLLT had synergistic effects on osteogenic differentiation. The results indicated that CGM promoted proliferation, differentiation, and mineralization of osteoblasts at the threshold concentration of $10{\mu}g/ml$; whereas, CGM showed cytotoxic properties at concentrations above $100{\mu}g/ml$. ALP activity and mineralization were increased at concentrations above $10{\mu}g/ml$. CGM in concentrations up to $10{\mu}g/ml$ also increased the expression of osteoblast-activated factors including type I collagen, BMP-2, RUNX2, and Osterix. The CGM ($50{\mu}g/ml$) and LLLT (80 mW for 15 sec) combination treatment group showed the highest proliferation levels, ALP activity, and mineralization ratios. The combination treatment also increased the levels of phosphorylated forms of p38, ATF2, PKD, ERK, and JNK. In addition, the osteoblast differentiation factors including type I collagen, BMP-2, RUNX2, and Osterix protein levels were clearly increased in the combination treatment group. These results suggested that the combination treatment of CGM and LLLT has synergistic effects on the differentiation and mineralization of osteoblastic cells.

• Journal of the Society of Disaster Information
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• v.7 no.2
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• pp.87-95
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• 2011

The skid resistant of pavement surface is an important parameter since it is directly related to the traffic safety under moving vehicular loads. In particular, it should be considered as a major factor in pavement performance evaluations to reduce the traffic accident from vehicular sliding. In this study, a portable and an automatic skid resistance tests were used to evaluate the skid resistances of the in-situ pavements. The test results showed that the skid resistance of the conventional dense graded pavement was more noticeable than the other pavement types such as the drainage pavement and the stone mastic asphalt(SMA) pavement as the service life of pavement was increased.

• International Journal of Highway Engineering
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• v.11 no.4
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• pp.25-31
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• 2009

In general, it is well known fact that the stone mastic asphalt (SMA) pavement has a high resistance against rutting. However, performance of SMA is not well measured by general method used in the laboratory. The objective of this study is to investigate an applicability of deformation strength ($S_D$) for performance estimation of SMA, and to find out the correlation between rut depth and dynamic stability, and $S_D$ of SMA. This study carried out wheel tracking test and Kim-test with optimum asphalt content (OAC) determined by mix design. The results indicated that the $S_D$ of SMA was very poorer than those of dense-graded asphalt mixtures. $S_D$ showed similar WT dynamic stability and rut-depth level. It was found that Kim-test was not reflected higher rutting resistance of SMA like as indirect tensile strength (ITS) test and Marshall stability test. Also, it was revealed that dynamic stability and rut-depth of WT had some problems to estimate rutting resistance of SMA mixtures.

• Journal of the Korea Institute of Building Construction
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• v.19 no.1
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• pp.19-29
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• 2019

In this study, a revised oil leakage evaluation method for assessing oil leakage stability of asphalt mastics used in upper slabs of below-grade residential parking lots was developed and presented. In order to verify the reliability and reproducibility of leakage results, the parameters the revised evaluation was carried out for three products with actual leakage history, and it was confirmed the leaks could be reproduced whereas the existing methods could not. To quantitatively verify the reproducibility, the filler content of the leaked samples was derived and the maximum filler content was 64% lower than that of the normal sample. The same results was found with the samples from the actual leakage site, thus verifying the reliability of the revised evaluation method.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.299-304
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• 2003

In this research, eight waterproofing membranes were selected from commercial market and the tensile adhesive characteristics of the waterproofing system (WPS) on concrete bridge deck were investigated in view of various factor in asphalt pavement. Tensile adhesive strength (TAS) test of different asphalt pavement types showed that TAS of WPS under SMA (Stone Mastic Asphalt) pavement was greater than that under dense asphalt pavement. Tensile adhesive strength (TAS) of sheet membranes was improved as the compaction temperature of asphalt concrete increase, but TAS of liquid membranes were not. TAS of sheet membranes after wheel tracking test were in the order of the sites under wheel path (UWP), before wheel tracking (BWT) and nearby wheel path (NWP), Since TAS of the same WPS of UWP was higher than TAS of BWT, wheel loading had function of pressing WPS resulting in higher adhesive strength, But liquid membranes were variable on types, The feature of detached interface after T AS test showed that sheet types were all detached in between deck concrete and WPS, and liquid types were detached in between asphalt pavement and WPS,

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.3D
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• pp.421-431
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• 2011

In this paper, actual bridges constructed with SMA (Stone Mastic Asphalt) deck pavement and virtual bridges substituted the deck pavement with polymer concrete under the same conditions were statically analyzed to investigate applicability of the thin polymer concrete bridge deck pavements. PSC (prestressed Concrete) girder bridge, steel box girder bridge, PSC box girder bridge, and RC (Reinforced Concrete) rahmen bridge constructed with the SMA deck pavement were analyzed and compared to evaluate various types of the bridge. The bridge deck and pavement were assumed to be fully bonded and the stress and deformation during the construction were ignored while those due to pavement weight and vehicle loading were analyzed. According to the analysis results, the stress and deformation of the bridges using the polymer concrete due to the pavement weight were smaller than those using the SMA because of smaller self weight due to lighter unit weight and thinner thickness of the pavement. The stress and deformation of the bridges using the polymer concrete due to the vehicle loading were larger than those using the SMA because of the smaller area moment of inertia due to the thinner pavement thickness. In case that the pavement weight and vehicle loading applied simultaneously, the stress and deformation of the bridges using the polymer concrete were smaller because effect of self weight reduction was more dominant. Investigation of performance of the bridge deck pavement and analysis of economical efficiency are warranted.

• Journal of Advanced Research in Ocean Engineering
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• v.1 no.4
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• pp.211-226
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• 2015

Membrane type Mark III cargo containment system (CCS) is considered in this study to investigate its strength capability under applied loads due to liquefied natural gas (LNG) cargo. A rectangular plated structure supported by inner hull structure is exemplified from Mark III CCS according to classification society's guidance and it is assumed as multi-layered structure by stacking plywood, triplex, reinforced polyurethane (PU) foam and series of mastic upon inner hull structure. Commercially available general purpose finite element analysis package is used to have reliable FE models of Mark III CCS plate. The FE models and anisotropic failure criteria such as maximum stress, Hoffman, Hill, Tsai-Wu and Hashin taking into account the direction dependent material properties of Mark III CCS plate components and their material properties considering a wide variation of temperature due to the nature of LNG together form the strength analysis procedure of Mark III CCS plate. Strength capability of Mark III CCS plate is understood by its initial failure and post-initial failure states. Results are represented in terms of failure loads and locations when initial failure and post-initial failures are occurred respectively. From the results the basic design information of Mark III CCS plate is given.