• KSBB Journal
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• v.22 no.2
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• pp.91-96
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• 2007

The saccharogenic liquid (SFW) obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes was carried out by the cultivation supernatant of Tricoderma inhamatum KSJ1 culture. Acetobacter xylinum KJ1 was employed for the BC production culture. Under the scaled-up aeration condition of 1.0 vvm, 5.64 g/L of BC was produced in 3 days cultivation in 50 L air circulation bioreactor using SFW medium with addition of 0.4% agar. The productivity was similar to that of 10 L air circulation bioreactor (5.84 g/L). This cultivation method with 50 L air circulation bioreactor decreasing shear stress and increasing oxygen transfer coefficient ($k_La$) was very useful in BC mass production. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BC produced by the static culture (A), the air circulation culture using 10 L bioreactor (B) and 50 L bioreactor (C) were investigated. The number average molecular weight of BCs produced under the different culture conditions (A-C) showed 2,578,000, 1,975,000, and 1,809,000, respectively. Tensile strength was 1.72 $kg/mm^2$, 1.19 $kg/mm^2$, and 1.18 $kg/mm^2$, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 86.2$\sim$87.8%. BC production by the air circulation culture mode brought more favorable results in terms of the physical properties and its ease of scale-up. Therefore, it is expected that the new BC production method, the air circulation culture using SFW, would contribute greatly to BC-related manufacturing.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.13 no.4
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• pp.99-105
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• 2014

The domestic airline industry undertakes the production of finished products by assembling existing self-described components via a design process which involves assembly and production steps, after which many of the finished products are exported. However, high reliability and stability must be guaranteed, because customers require high-precision components at the time of manufacturing. In the aircraft parts industry, the mass production of high-value-added parts is limited. Therefore, a small production scale depending on the part is used, as many types of conventional CNC lathe machines with X-axis and Z-axis as well as Z-axis and C-axis CNC milling are used. The parts also rely on high-pressure air to increase production. The most important factors are good stability during processing, as high-precision parts are required, as noted above. It was found that as the C-axis rotation speed increased, the diameter of the cutting tool decreased with a decrease in the surface roughness, while the workpiece rotation speed increased with an increase in the surface roughness.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.466-469
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• 1999

Cladocera are important food organism for seed production of finfishes. freshwater cladocera such as Daphnia and Moina are well known food organisms for the larval rearing of freshwater fishes and are easy for mass culture. However, mass culture technique for marine cladocera are not yet developed, The only mass produced food organisms available these days for the larval production of marine finfishes are rotifer and Artemia. An estuarine cladoceran, Diaphanosoma celebensis, has a high possibility of being used as a food organism for the larval rearing of marine finfishes because this species is much easier to mass culture than marine ones. Therefore many studies are needed for this species. In this study, the effects of the volumes of culture container, 40, 1,500 and 15,000 ml, on the stable production of this species were tested and results are as follow: The maximum densities of this species in each of the culture volumes were reached after 14 days in 40 ml, 12 days in 1,500 ml, and 21 days in 15,000 ml with values of 3.4 $\pm$ 0.4, 14.2 $\pm$ 2.1 and 2.5 $\pm$ 1.6 per ml, respectively. The relative population growth index (RPGI) was stable in the culture volume of 1,500 ml. Moreover, possible harvesting number(individual/ml/day) was much higher in the 1,500 ml container than the other culture volumes. Therefore, optimum culture volume among the tested volumes for mass production of this species was 1,500 ml.

• KSBB Journal
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• v.15 no.4
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• pp.342-345
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• 2000

A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage size and complexity of the HPLC operations for peroxidase purification. This process is readily scalable to a pilot plant and eventually to a production environment where mass production of material are expected to be produced.

• Membrane and Water Treatment
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• v.6 no.6
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• pp.451-476
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• 2015

Membrane distillation (MD), which can utilize low-grade thermal energy, has been extensively studied for desalination. By incorporating solar thermal energy, the solar membrane distillation desalination system (SMDDS) is a potential technology for resolving the energy and water resource problems. Small-scale SMDDS (s-SMDDS) is an attractive and viable option for the production of fresh water for small communities in remote arid areas. The minimum-cost design and operation of s-SMDDS are determined by a systematic method, which involves a pseudo steady state approach for equipment sizing and the dynamic optimization using overall system mathematical models. The s-SMDDS employing three MD configurations, including the air gap (AGMD), direct contact (DCMD) and vacuum (VMD) types, are optimized. The membrane area of each system is $11.5m^2$. The AGMD system operated for 500 kg/day water production rate gives the lowest unit cost of $5.92/m^3$. The performance ratio and recovery ratio are 0.85 and 4.07%, respectively. For the commercial membrane employed in this study, the increase of membrane mass transfer coefficient up to two times is beneficial for cost reduction and the reduction of membrane heat transfer coefficient only affects the cost of the DCMD system.

• Journal of Dairy Science and Biotechnology
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• v.39 no.3
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• pp.87-93
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• 2021

This study analyzed and compared growth characteristics under large-scale fermentation at 35℃ of three microorganisms with the ability to reduce odor-producing substances in livestock. The three microorganisms (Bacillus subtilis FWC1, Bacillus amyloliquefaciens NAAS1, and Pichia farinosa NAAS2) evaluated in this study have been proven effective in reducing odor-inducing substances. Bacillus subtilis FWC1 exhibited the highest viable cell count when using 2% maltodextrin as carbon source, 0.05% soy-peptone as nitrogen source, and 0.3% yeast extract. The optimum media composition for B. amyloliquefaciens NAAS1 was 1.2% modified-starch with 0.8% yeast extract. The spore formation rate in the mass production of the Bacillus strains was over 90%, indicating that optimal growth medium compositions have been identified. In the case of P. farinosa NAAS2, our optimized growth medium [2% (w/v) glucose and 1% (w/v) yeast extract] improved biomass production.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.2
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• pp.217-220
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• 2002

Mulberry being a foliage crop is grown extensively for feeding of silkworms and are also used for cattle feeding. These loaves are highly nutritious, which contain various mineral elements and bio-molecules such as carbohydrates, proteins, lipids and other essential amino acids, etc. In the present study, deproteinized mulberry leaf juice was used for preparation of the medium for cultivation of various types of microbes. Results revealed that deproteinized mulberry leaf juice medium is best for isolation of fungi, bacteria and actinomycetes and this medium can be substituted with synthetic media, which are haying the costly ingredients for isolation and identification of bacteria, fungi and actinomycetes. Further, this deproteinized mulberry juice medium can also be used in mass multiplication of useful/beneficial microbes to enhance soil microflora to improve soil fertility and to avoid root diseases. Perspective enterprises can take up the mass multiplication/large-scale production of useful microbes such as Trichoderma, Rhizobium, Pseudomonas and Bacillus to use in mulberry and in other agricultural crops using deproteinized mulberry leaf juice.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.221-227
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• 2015

We analyzed the small-scale purse-seine gear that is used along the North Coast of Java, Indonesia, using computer-aided tools to modify the gear. Data from the middle position of the leadline showed that the maximum depth reached by the net was 30 m. A similar result was also calculated. According to the calculated result, the mean sinking speed of the current gear at the middle position of the leadline was 0.13 m/s, and the maximum tension during pursing was 1,794 kgf. The best sinking performance was found in modified gear that used a 30.3 mm mesh knotless polyester net. The maximum depth reached by the net was 38 m, and mean sinking speed was 0.16 m/s at the middle position of the leadline. The maximum tension during pursing was 1,044 kgf. Independent sample t-test results show that the mean sinking depth and sinking speed in the simulated and measured results did not differ (P > 0.05). These results are expected to improve the efficiency and selectivity of small-scale purse seine gear.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.1511-1516
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• 2009

In recent years, the large-scale shaft have been appling in domestic mine for mass production using a large machine, the safety of mine also have been increasing. And the new trial that shotcrete of tunnel field was apply to mine support was progressed. But, the conditions of domestic mine was different from that of tunnel, so, the batch plant of tunnel could not be installed in mine field because of low economical efficiency and difficulty for selection of site. Ready-mixed Shotcrete that mixed with high quality materials and could be controled shotcrete quality is producted in plants and transported to field, so do not need to batch plant. In this study, The Field test of the Ready-mixed Shotcrete was performed in the large-scale mine and was compared with the quality of Field mix shotcrete. As the result of the Field test, compressive strength and rebound of Ready-mixed Shotcrete were superior to these of Field mix shotcrete.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.4
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• pp.245-249
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• 2007

High quality of purified alginate and fucoidan is required for the medical uses to prevent the unexpected side effects from the contaminated endotoxin present in the materials. We attempted to establish an efficient and fast mass scale production method for the highly purified poly-G alginate from the sea weeds. In addition, lab scale method was established to obtain the fraction of higher purity from the commercially available fucoidan source (about 85% purity).