• Title/Summary/Keyword: mass spectral method

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PROPERTIES OF DUST IN EARLY-TYPE GALAXIES BASED ON THE ALL-SKY-SURVEY DATA AND NEAR-INFRARED SPECTRA

  • Mori, T.;Oyabu, S.;Kaneda, H.;Ishihara, D.;Yamagishi, M.
    • Publications of The Korean Astronomical Society
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    • v.27 no.4
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    • pp.263-264
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    • 2012
  • We present the properties of dust and the near-infrared spectral features in nearby early-type galaxies. The properties of dust are obtained from the AKARI far-infrared all-sky survey diffuse map. The AKARI/IRC is used for the near-infrared spectra. We improve spectral data with the new dark subtraction method on the basis of the knowledge acquired in our laboratory experiments of the engineering-model detector for the IRC. We have succeeded in fitting the continuum by a power-law function and detecting CO and SiO absorption features in early-type galaxy spectra. Comparing the properties of dust and near-infrared spectral features, we find that the power-law slope depends on dust temperature, but not on the dust mass, which suggests that low-luminosity AGNs may contribute to the changes in the power-law slope and dust temperature.

Mass function of star clusters in the nuclear starburst region of NGC 253

  • Lim, Sungsoon;Lee, Myung Gyoon
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.1
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    • pp.39.2-39.2
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    • 2014
  • We present a photometric study of star clusters in the nuclear starburst region of NGC 253 using gVI, YJ, and H band images in the Hubble Space Telescope archive. We find about one thousand star clusters in about 200"x200" field by visual inspection with I<21. We also find about ten thousand star clusters in the same field by automated classification method with magnitude range of 21< I $$\leq_-$$ 24. Ages and masses of star clusters are estimated using spectral energy distribution fitting method. Age distribution of star cluster shows two distinguished young populations with peak ages at 3.5 Myr and 18 Myr. Old populations (>100 Myr) are exist, but their number is small. About thirty young massive star clusters (<10 Myr, ) are found in nuclear region of NGC 253 which are regarded as a result of the recent starburst. Mass function of young star clusters in NGC 253 is somewhat different with those of star clusters in other galaxies. This result suggests that initial cluster mass functions (ICMFs) for star clusters are not universial. Especially ICMF in starburst galaxies may be distinguishable compared with those in normal spiral galaxies. We discuss the implications of these results.

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Evaluation of MALDI Biotyping for Rapid Subspecies Identification of Carbapenemase-Producing Bacteria via Protein Profiling

  • Somboro, Anou M.;Tiwari, Dileep;Shobo, Adeola;Bester, Linda A.;Kruger, Hendrik G.;Govender, Thavendran;Essack, Sabiha Y.
    • Mass Spectrometry Letters
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    • v.5 no.4
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    • pp.110-114
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    • 2014
  • The method of direct mass spectrometry profiling is reliable and reproducible for the rapid identification of clinical isolates of bacteria and fungi. This is the first study evaluating the approach of MALDI-TOF mass spectrometry profiling for rapid identification of carbapenemase-resistant enterobacteriaceae (CRE). Proof of concept was achieved by the discrimination of CRE using MALDI Biotyper MS based on the protein. This profiling appears promising by the visual observation of consistent unique peaks, albeit low intensity, that could be picked up from the mean spectra (MSP) method. The Biotyper MSP creation and identification methods needed to be optimized to provide significantly improved differences in scores to allow for subspecies identification with and without carbapenemases. These spectra were subjected to visual peak picking and in all cases; there were pertinent differences in the presence or absence of potential biomarker peaks to differentiate isolates. We also evaluated this method for potential discrimination between different carbapenemases bacteria, utilizing the same strategy. Based on our data and pending further investigation in other CREs, MALDI-TOF MS has potential as a diagnostic tool for the rapid identification of even closely related carbapenemases but would require a paradigm shift in which Biotyper suppliers enable more flexible software control of mass spectral profiling methods.

Volatile flavor components of Korean ginger(Zingiber officinale Roscoe)extracted with liquid carbon dioxide (액체 이산화탄소 추출법에 의한 생강 (Zingiber officinale Roscoe)의 향기성분)

  • Kim, Myung-Kon;Na, Mun-Su;Hong, Jai-Sik;Jung, Soon-Taek
    • Applied Biological Chemistry
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    • v.35 no.1
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    • pp.55-63
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    • 1992
  • The essential oil of Korean ginger(Zingiber officinale Roscoe) was isolated by liquid carbon dioxide extraction method and fractionated into one hydrocarbon fraction and two oxygenated hydrocarbon fractions by using silica gel column chromatography. The compositions of the resulting oils were investigated by GC and GC-MS spectrometry. Out of 102 identified compounds, 44 were identified by comparing GC retention time and mass spectral data with authentic samples and 58 were tentatively identified according to mass spectral data only. The major compounds of hydrocarhon fraction were $zingiberene,\;{\beta}-sesquiphellandrene,\;{\gamma}-bisabolene,\;{\gamma}-cardinene,\;ar-curcumene$, and those of oxygenated hydrocarbon fractions wee geranal, sesquisabinene hydrate, borneol and zingiberenol. The major compounds of ginger oil were zingiberene, $citronellol+{\beta}-sesquiphellandrene,\;geranial,\;{\gamma}-bisabolene\;and\;ar-curcumene+geranyl\;acetate$, and ginger oil contained higher amounts of sesquiterpene hydrocarbons. The yield of extract was 6.96%.

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Improvement of protein identification performance by reinterpreting the precursor ion mass tolerance of mass spectrum (질량스펙트럼의 펩타이드 분자량 오차범위 재해석에 의한 단백질 동정의 성능 향상)

  • Gwon, Gyeong-Hun;Kim, Jin-Yeong;Park, Geon-Uk;Lee, Jeong-Hwa;Baek, Yung-Gi;Yu, Jong-Sin
    • Bioinformatics and Biosystems
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    • v.1 no.2
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    • pp.109-114
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    • 2006
  • In proteomics research, proteins are digested into peptides by an enzyme and in mass spectrometer, these peptides break into fragment ions to generate tandem mass spectra. The tandem mass spectral data obtained from the mass spectrometer consists of the molecular weights of the precursor ion and fragment ions. The precursor ion mass of tandem mass spectrum is the first value that is fetched to sort the candidate peptides in the database search. We look far the peptide sequences whose molecular weight matches with precursor ion mass of the mass spectrum. Then, we choose one peptide sequence that shows the best match with fragment ions information. The precursor ion mass of the tandem mass spectrum is compared with that of the digested peptides of protein database within the mass tolerance that is assigned by users according to the mass spectrometer accuracy. In this study, we used reversed sequence database method to analyze the molecular weight distribution of precursor ions of the tandem mass spectra obtained by the FT LTQ mass spectrometer for human plasma sample. By reinterpreting the precursor ion mass distribution, we could compute the experimental accuracy and we suggested a method to improve the protein identification performance.

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Explicit Transient Simulation of SH-waves Using a Spectral Element Method (스펙트럴 요소법을 이용한 SH파 전파의 외연적 시간이력해석)

  • Youn, Seungwook;Kang, Jun Won
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.31 no.2
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    • pp.87-95
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    • 2018
  • This paper introduces a new explicit spectral element method for the simulation of SH-waves in semi-infinite domains. To simulate the wave motion in unbounded domains, it is necessary to reduce the infinite extent to a finite computational domain of interest. To prevent the wave reflection from the trunctated boundaries, perfectly matched layer(PML) wave-absorbing boundary is introduced. The forward problem for simulating SH-waves in PML-truncated domains can be formulated as second-order PDEs. The second-order semi-discrete form of the governing PDEs is constructed by using a mixed spectral elements with Legendre-gauss-Lobatto quadrature method, which results in a diagonalized mass matrix. Then the second-order semi-discrete form is transformed to a first-order, whose solutions are calculated by the fourth-order Runge-Kutta method. Numerical examples showed that solutions of SH-wave in the two-dimensional analysis domain resulted in stable and accurate, and reflections from truncated boundaries could be reduced by using PML boundaries. Elastic wave propagation analysis using explicit time integration method may be apt for solving larger domain problems such as three-dimensional elastic wave problem more efficiently.

Vibration Analysis of Frame Structural Systems by the Receptance Method

  • Han, S.Y.;Kim, K.C.
    • Selected Papers of The Society of Naval Architects of Korea
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    • v.1 no.1
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    • pp.52-64
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    • 1993
  • There exist many frame structural systems having some attachments reducible to damped spring-mass systems, concentracted masses and spring supports. For free and forced vibration analyses of such a system an analytical method based on the receptance method is presented. A framed structure having attachments is considered as a combined system composed of various Timoshenko beam and bar elements and the attachments. So, the vibration characteristics of the system are calculated by synthesizing receptances and Support Displacement Transfer Ratio (SDTR) of beam and bar elements In spectral and/or closed forms, and receptances of the attachments. In forced vibration analysis, arbitrary excitation forces at a point on the structure and displacement excitations at boundaries are considered. Numerical investigations are carried out for verification of the presented method, and the results show good accuracy and very high computational efficiency.

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Reflectance of Geological Media by Using a Field spectrometer in the Ungsang Area, Kyungsang Basin

  • Kang, Kyung-Kuk;Song, Kyo-Young;Ahn, Chung-Hyun;Won, Joong-Sun
    • Korean Journal of Remote Sensing
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    • v.17 no.2
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    • pp.165-181
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    • 2001
  • Using a field spectrometer having a spectral range of 0.4$\mu\textrm{m}$~2.5$\mu\textrm{m}$ with a spectral resolution of 1nm, the researchers measured the reflectance of granite, andesitic rocks, sedimentary rocks, and pyrophyllite ore in the Ungsang area, Kyungsang Basin, South Korea. Spectral characteristics of the geological media were investigated from the analysis. The in-situ measured sites were selected in well exposed rock outcrops. In case of unfavorable weather conditions, rocks were sampled and remeasured under natural solar condition. The reflectance of field data was measurd at three sistes for granite, six sites for andesitic rock three sites for sedimentary rocks, and two sites for pyrophyllite ore. The vibrational absorption bands for pyrophyllite are detected in the spectral range of 2.0$\mu\textrm{m}$~2.5$\mu\textrm{m}$. The absorption band for granites in study area is not distinctive. The reflectance measured under normal field conditions showed strong absorption at wavelengths of 1.4$\mu\textrm{m}$ and 1.9$\mu\textrm{m}$ due to the effect of moisture in the atmosphere. After the bands of 1.4$\mu\textrm{m}$ and 1.9$\mu\textrm{m}$ were removed, Hull Quotient method was applied to characterize absorption bands. The reflectances of field data were calculated to estimate the band ratio corresponding to the Landsat TM and EOS Terra ASTER. The researchers suggest here that the TM band2, band3, band4, and band7 or ASTER band2, band3, band4, and band9 are the best combination for discriminating outcrops. The researchers tested and demonstrated using a Landsat TM image in the study area. For geologic applications, decorrelation stretch is also an effective tool to enhance the exposed rock mass in images.

Analysis of Volatile Components of Sancho(Zanthoxylum schinifolium)by Solid Phase Micro Extraction (Solid Phase Micro Extraction을 이용한 산초의 휘발성 성분 분석)

  • 장희진
    • The Korean Journal of Food And Nutrition
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    • v.12 no.2
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    • pp.119-123
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    • 1999
  • Test of the optimum condition of solid phase micro extraction(SPME) was performed by use of 5 vol-atile components in dilute aqueous solution. Volatile components of Sancho(Zanthoxylum schinifolium) were isolated by SPME method and were analyzed by GC/MSD and compared with volatile compone-nts isolated by simultaneous distillation extraction (SDE) method. Total 31 components were identified by comparing gas chromatography retention time and mass spectral data. The major compounds were limonene geranyl acetate $\beta$-phellandrene phellandral mycene linalool rose oxide caproic acid and caprylic acid SPME sampling procedure was found to be a good method for qualitative analysis of the volatile components.

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Stochastic response spectra for an actively-controlled structure

  • Mochio, Takashi
    • Structural Engineering and Mechanics
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    • v.32 no.1
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    • pp.179-191
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    • 2009
  • A stochastic response spectrum method is proposed for simple evaluation of the structural response of an actively controlled aseismic structure. The response spectrum is constructed assuming a linear structure with an active mass damper (AMD) system, and an earthquake wave model given by the product of a non-stationary envelope function and a stationary Gaussian random process with Kanai-Tajimi power spectral density. The control design is executed using a linear quadratic Gaussian control strategy for an enlarged state space system, and the response amplification factor is given by the combination of the obtained statistical response values and extreme value theory. The response spectrum thus produced can be used for simple dynamical analyses. The response factors obtained by this method for a multi-degree-of-freedom structure are shown to be comparable with those determined by numerical simulations, demonstrating the validity and utility of the proposed technique as a simple design tool. This method is expected to be useful for engineers in the initial design stage for structures with active aseismic control.