• Title/Summary/Keyword: mass of the suspension

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Micropropagation of Cassava by Suspension Culture Derived from its Nodal Explants (마디 절편의 현탁배양에 의한 카사바의 미세증식)

  • Yoon, Sil;Cho, Duck-Yee;Soh, Woong Young
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.185-189
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    • 2000
  • For the micropropagation, node explants of cassava were cultured in liquid MS medium with various concentrations of cytokinins on a rotary shaker (100 rpm) for 2 weeks. The adventitious roots and shoots from the explants were differentiated more efficiently in liquid medium than in solid. But root formation was not inhibited in medium with BAP and kinetin at low concentration (>0.05 mg 1/sup -1/), while in medium added with BAP and zeatin at high level (<0.25 mg 1/sup -1/), it was inhibited by callus forming on cut end of the cuttings. However, all of plantlets grown in liquid medium for more than 2 weeks showed symptoms of hyperhydricity. The plantlets grown in liquid medium were transferred into culture bottles filled with fine sand or artificial soil (pitmoss:perlite:vermiculite, 1:1:1 v/v) wetted with half strength of Knop's solution. After transplanted to culture bottles, some of vitriscent leaves were defoliated and new leaves were normally formed from shoot apex. Most of plantlets (>95%) were hardened-off successfully only in culture bottles with fine sand, and grew into 3-5 cm seedlings possessing 4-6 nodes after 4 weeks. Thus, the mass propagation of cassava on medium containing cytokinin could be established based on the suspension culture using node explants.

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A numerical application of Bayesian optimization to the condition assessment of bridge hangers

  • X.W. Ye;Y. Ding;P.H. Ni
    • Smart Structures and Systems
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    • v.31 no.1
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    • pp.57-68
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    • 2023
  • Bridge hangers, such as those in suspension and cable-stayed bridges, suffer from cumulative fatigue damage caused by dynamic loads (e.g., cyclic traffic and wind loads) in their service condition. Thus, the identification of damage to hangers is important in preserving the service life of the bridge structure. This study develops a new method for condition assessment of bridge hangers. The tension force of the bridge and the damages in the element level can be identified using the Bayesian optimization method. To improve the number of observed data, the additional mass method is combined the Bayesian optimization method. Numerical studies are presented to verify the accuracy and efficiency of the proposed method. The influence of different acquisition functions, which include expected improvement (EI), probability-of-improvement (PI), lower confidence bound (LCB), and expected improvement per second (EIPC), on the identification of damage to the bridge hanger is studied. Results show that the errors identified by the EI acquisition function are smaller than those identified by the other acquisition functions. The identification of the damage to the bridge hanger with various types of boundary conditions and different levels of measurement noise are also studied. Results show that both the severity of the damage and the tension force can be identified via the proposed method, thereby verifying the robustness of the proposed method. Compared to the genetic algorithm (GA), particle swarm optimization (PSO), and nonlinear least-square method (NLS), the Bayesian optimization (BO) performs best in identifying the structural damage and tension force.

Sediment Erosion and Transport Experiments in Laboratory using Artificial Rainfall Simulator

  • Regmi, Ram Krishna;Jung, Kwansue;Nakagawa, Hajime;Kang, Jaewon;Lee, Giha
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.4
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    • pp.13-27
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    • 2014
  • Catchments soil erosion, one of the most serious problems in the mountainous environment of the world, consists of a complex phenomenon involving the detachment of individual soil particles from the soil mass and their transport, storage and overland flow of rainfall, and infiltration. Sediment size distribution during erosion processes appear to depend on many factors such as rainfall characteristics, vegetation cover, hydraulic flow, soil properties and slope. This study involved laboratory flume experiments carried out under simulated rainfall in a 3.0 m long ${\times}$ 0.8 m wide ${\times}$ 0.7 m deep flume, set at $17^{\circ}$ slope. Five experimental cases, consisting of twelve experiments using three different sediments with two different rainfall conditions, are reported. The experiments consisted of detailed observations of particle size distribution of the out-flow sediment. Sediment water mixture out-flow hydrograph and sediment mass out-flow rate over time, moisture profiles at different points within the soil domain, and seepage outflow were also reported. Moisture profiles, seepage outflow, and movement of overland flow were clearly found to be controlled by water retention function and hydraulic function of the soil. The difference of grain size distribution of original soil bed and the out-flow sediment was found to be insignificant in the cases of uniform sediment used experiments. However, in the cases of non-uniform sediment used experiments the outflow sediment was found to be coarser than the original soil domain. The results indicated that the sediment transport mechanism is the combination of particle segregation, suspension/saltation and rolling along the travel distance.

Optimal variables of TMDs for multi-mode buffeting control of long-span bridges

  • Chen, S.R.;Cai, C.S.;Gu, M.;Chang, C.C.
    • Wind and Structures
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    • v.6 no.5
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    • pp.387-402
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    • 2003
  • In the past decades, much effort has been made towards the study of single-mode-based vibration controls with dynamic energy absorbers such as single or multiple Tuned Mass Dampers(TMDs). With the increase of bridge span length and the tendency of the bridge cross-section being more slender and streamlined, multi-mode coupled vibrations as well as their controls have become very important for large bridges susceptible to strong winds. As a simple but effective device, the TMD system especially the semi-active one has become a promising option for such coupled vibration controls. However, despite various studies of optimal controls of single-mode-based vibrations with TMDs, research on the corresponding controls of the multi-mode coupled vibrations is very rare so far. For the development of a semi-active control strategy to suppress the multi-mode coupled vibrations, a comprehensive parametric analysis on the optimal variables of this control is substantial. In the present study, a multi-mode control strategy named "three-row" TMD system is discussed and the general numerical equations are developed at first. Then a parametric study on the optimal control variables for the "three-row" TMD system is conducted for a prototype Humen Suspension Bridge, through which some useful information and a better understanding of the optimal control variables to suppress the coupled vibrations are obtained. This information lays a foundation for the design of semi-active control.

Production of Single-Cell Protein from Starchy Material by the Fusant (전분이용성 세포융합 효모를 이용한 단세포단백질 생산)

  • 정건섭;최신양;구영조;신동화
    • Microbiology and Biotechnology Letters
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    • v.16 no.2
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    • pp.105-110
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    • 1988
  • The production of single cell protein using the amylolytic fusant obtained from cell fusion between Hansenula anomala and Saccharomyces cerevisiae was studied. The fusant12 strain was selected for single cell protein production from starchy materials among five fusants. Optimum nitrogen source and its concentration for the growth of fusant12 were ammonium sulfate and 0.1%, respectively. Optimum concentration of soluble starch and optimum pH of the basal medium were lord and pH 5.6, respectively. Autolysis of fusant12 was effectively carried out by addition of 5% (v/v) ethyl acetate to the cell suspension and liquidization for 30 min before incubation for 24 hr at 3$0^{\circ}C$. Coculture of fusant12 and non-amylolytic yeast, Torulopsis candida YA-l5, resulted in the increase of the mass as compared to the monoculture of fusant12. The cell mass on tapioca medium was increased about 2.5 times as on soluble starch medium. The content of crude protein and nucleic acid of the dry cell were 39% and 5.8%, respectively.

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A Numerical Study on Shear Behavior of the Interface between Blasted Rock and Concrete (발파 암반-콘크리트 경계면에서의 전단거동특성에 대한 수치해석적 연구)

  • Min, Gyeong-Jo;Ko, Young-Hun;Fukuda, Daisuke;Oh, Se-Wook;Kim, Jeong-Gyu;Chung, Moon-Kyung;Cho, Sang-Ho
    • Explosives and Blasting
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    • v.37 no.4
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    • pp.26-35
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    • 2019
  • In designing a gravity-type anchorage of earth-anchored suspension bridge, the contact friction between a blasted rock mass and the concrete anchorage plays a key role in the stability of the entire anchorage. Therefore, it is vital to understand the shear behavior of the interface between the blasted rock mass and concrete. In this study, a portable 3D LiDAR scanner was utilized to scan the blasted bottom surfaces, and rock surface roughness was quantitatively analyzed from the scanned profiles to apply to 3D FEM modelling. In addition, based on the 3D FEM model, a three-dimensional dynamic fracture process analysis (DFPA-3D) technique was applied to study on the shear behavior of the interface between blasted rock and concrete through direct shear tests, which was analyzed under constant normal load (CNL). The effects of normal stress and the joint roughness on shear failure behavior are also analyzed.

Simple Mass-screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici (토마토 시들음병에 대한 간편 대량 저항성 검정법)

  • Park, Myung Soo;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.110-116
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    • 2013
  • This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than $1{\times}10^7\;conidia{\cdot}mL^{-1}$) of FOL was poured directly on the roots. The infected plants were cultivated in a growth room at $25-30^{\circ}C$ for 4 weeks with 12-hours light a day.

A Study on the Factors Affecting the High Fluid Mortar Containing Ground Granulated Blast-furnace Slag (고로슬래그 미분말을 함유한 고유동 모르터의 유동성상에 미치는 영향 요인에 관한 연구)

  • 김재훈;윤상천;지남용
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2002.11a
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    • pp.29-36
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    • 2002
  • High fluid concrete unlike OPC concrete is made with various material, and the phase of fresh concrete is considerably different. In order to understand fluidity phase and mix properties of high fluid concrete, concrete is required to access as suspension structure which consists of aggregate and paste. The focus of this paper is to analyze the test results and quantify the effect of mix proportions of molar and fineness modulus of ,and on the properties of fresh mortar. The effect of water-binder ratio, sand-binder ration, content; of ggbs (by mass of total cementitious materials), and various contents of water reducing agent on the yield stress and plastic viscosity of the mix is studied. Based on the experimental results, the following conclusion; can be drawn: (1) The mixing time needed (or high fluid mortar was approximately two times more than that of ordinary portland mortar. (2) The fluidity phase of mortar could be explained by yield stress of mix and the fluidity of mortar. (3) As the content of ggbs increased, yield stress of mortar was decreased and plastic viscosity of it was increased. (4) For the high fluid mortar, it was appeared that sand-binder ratio should be below 1.5.

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Primary Squamous Cell Carcinoma of the Posterior Glottis (성문후부(Posterior Glottis)에서 원발한 편평상피세포암종 1례)

  • 조승호;김형태;조재홍;김민식
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.9 no.2
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    • pp.160-163
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    • 1998
  • The glottis consists of two parts : The intermembranous portion or the anterior glottis, and the posterior glottis. The posterior glottis has been described by various inappropriate terms such as posterior commissure and interarytenoid region. The structure surrounding the posterior glottis consists of three portions ; The cartilaginous portion of the vocal fold, the lateral wall of the posterior glottis, and the posterior wall of the glottis. The posterior glottis may be a very rare site for primary squamous cell carcinoma of the larynx because of its embriologic and histologic differences from the anterior glottis. Recently we have experienced a case of a 81-year-old woman who had been presented with throat discomfort and voice change for 10months. She had the smoking history of 40 pack-year. The physical examination revealed poorly demarcated, papillary, whitish-pink colored mass on the posterior glottis. The vocal cord mobility was not affected. Biopsy under the suspension larygoscope showed moderately differentiated squamous cell carcinoma. No regional and distant metastasis was found. She was irradiated with 7000cGy over 7 weeks at a daily dose of 200cGy. No evidence of recurrence was found by the fifteenth month following radiation therapy. We report this case with a review of literature.

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Model-Driven Design Framework for Future Combat Vehicle Development based on Firepower and Mobility: (1) Integrated Performance Modeling (화력과 기동의 통합성능을 고려한 미래 전투차량의 해석 기반 설계 프레임웍 연구: (1) 통합성능분석 모델개발)

  • Lim, Sunghoon;Lim, Woochul;Min, Seungjae;Lee, Tae Hee;Ryoo, Jae Bong;Pyun, Jai-Jeong
    • Korean Journal of Computational Design and Engineering
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    • v.19 no.4
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    • pp.316-323
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    • 2014
  • This paper proposes the 3D modeling and simulation technique for predicting the integrated performance of combat vehicle. To consider the practical driving and firing condition of a combat vehicle, the full vehicle model, which can define the six degrees-of-freedom of vehicle motion and various firing angles, is developed. The critical design parameters such as the stiffness and damping coefficient of suspension system are applied to construct the analysis model of vehicle. A simple ballistic model, which incorporates the empirical interior ballistic model and the point mass trajectory model, is built to estimate the firing range and the firing recoil force. To predict the integrated performance and analyze the effect of system parameters, MATLAB/SIM-ULINK model of a combat vehicle for performing the real time simulation is also developed. Several simulation tests incorporating the road bump and the firing recoil force are presented to confirm the effectiveness of the proposed vehicle model.