This study aims at favorable environmental conditions by river otter residing in Hongcheon river ecosystems using spraints along the river. Otter spraints were indicative of habitat use pattern and marking a territory in the areas. Nae-chon and Kuneob-chon river otter spraints were collected totaling 478 with 8 times during 2009-2011, and based on the number river use patterns were analyzed with the technique of index of dispersion. Results with larger than one indicated that river otter habitat use pattern were not random; instead they used preferred areas for habitat use. 'I' values greater than one indicate a clumped distribution and lower than one indicate random distribution. This study also demonstrated that we need a sophiscated linear model that should be developed to identify key habitat elements in river ecosystems.
Unfortunately, there is no technique which is stable and repetitive to produce transgenic chicken, although various ways of gene transfer including PGC-and embryonic cell-mediated gene transfer, DNA microinjection, virus inoculation and sperm cells have been employed. The aims of this study were 세 develop and establish such a stable, repetitive and efficient way of gene transfer giving a faithful gene expression during development after the reconstruction of embryo in an UV-irradiated egg. A dual reporter plasmid (pJJ9), a fusion gene containing lacZ and GFP driven by a CMV promoter was used to exploit either merits of both reporting markers. lacZ with strong signal or GFP with vital marking. Electroporated embryonic blastodermal cells (EBCs) in the presence of the pJJ9 DNA faithfully showed 377 bp PCR product and lacZ or GFP expressions in the identical cells in vitro of in vivo. Furthermore, analyses of expression pattern of the foreign DNA demonstrated that microinjected EBCs cells into the UV-irradiated recipient egg should participate in normal developmental process, for example, proliferation and differentiation into various tissues. Thirty percentages of the manipulated eggs showed lacZ expression in their tissues. These results together with the specific procedures used in this study should facilitate avian transgenesis.
KSCE Journal of Civil and Environmental Engineering Research
/
v.39
no.5
/
pp.631-636
/
2019
Over the last decade, many researchers have investigated a number of vision-based construction object detection algorithms for the purpose of construction site monitoring. However, previous methods require the ground truth labeling, which is a process of manually marking types and locations of target objects from training image data, and thus a large amount of time and effort is being wasted. To address this drawback, this paper proposes a vision-based construction object detection framework that employs an active learning technique while reducing manual labeling efforts. For the validation, the research team performed experiments using an open construction benchmark dataset. The results showed that the method was able to successfully detect construction objects that have various visual characteristics, and also indicated that it is possible to develop the high performance of an object detection model using smaller amount of training data and less iterative training steps compared to the previous approaches. The findings of this study can be used to reduce the manual labeling processes and minimize the time and costs required to build a training database.
The current clinical technique for occlusal vertical dimension recording is based on marking the skin reference points on the patient's face and measuring between these points using caliper-like device. And it is difficult to achieve reliable measurements by this technique because of movable soft tissue. The purpose of this study is to reveal the stability of skin reference points by comparing the relative movement between extra-oral skin reference points and intra-oral reference points using X-ray fluoroscope. 10 test subjects were divided into 2 groups : Group I (natural dentition) and Group II (denture-wearer whose vertical dimension was lost) and Group III consists of identical test subjects to Group II with their upper denture removed and record base inserted. Attaching the 3 mm diameter steel ball to nose tip, lower lip, chin and to existing denture (or record base), fluoroscopic examination and recording were taken during 2 jaw opening and closing movements. After subsequent digitization using personal computer, 1219 still pictures with 0.1 second interval were made. Using the 2 dimensional graphic software, measurements between reference points were executed. Dividing the entire jaw movement into 3 ranges (total, 1st half opening, 2nd half opening), rate of movement and relative movement between extra-oral and intra-oral reference points were calculated and statistically analyzed. The results of this study are as follows. 1 Within the same experimental group, no statistical difference was found in the stability of skin reference between lower lip point and chin point during total range of jaw opening and closing movement (p>.05) 2. In the first half range of jaw opening, statistical difference was found between Group I (natural dentition) and Group II (denture wearer) (p<.05) Group I has greater skin reference stability than Group II. 3. In the first half range of jaw opening, statistical difference was found between Group I and Group III (record base wearer) (p<.05). Group I has greater skin reference stability than Group III. 4. In the first half range of jaw opening, no statistical difference was found in the stability of skin reference between Group II and Group III (p>.05). 5. In the second half range of jaw opening, no statistical difference was found in the stability of skin reference between any experimental groups (p>.05). 6. In patients with their occlusal vertical dimension lost, employing other measuring references rather than skin is recommended because of low stability.
Human hydatid disease (cystic echinococcosis, CE) is a chronic parasitic infection caused by the larval stage of the cestode Echinococcus granulosus. As the disease mainly affects the liver, approximately 70% of all identified CE cases are detected in this organ. Optical molecular imaging (OMI), a noninvasive imaging technique, has never been used in vivo with the specific molecular markers of CE. Thus, we aimed to construct an in vivo fluorescent imaging mouse model of CE to locate and quantify the presence of the parasites within the liver noninvasively. Drug-treated protoscolices were monitored after marking by JC-1 dye in in vitro and in vivo studies. This work describes for the first time the successful construction of an in vivo model of E. granulosus in a small living experimental animal to achieve dynamic monitoring and observation of multiple time points of the infection course. Using this model, we quantified and analyzed labeled protoscolices based on the intensities of their red and green fluorescence. Interestingly, the ratio of red to green fluorescence intensity not only revealed the location of protoscolices but also determined the viability of the parasites in vivo and in vivo tests. The noninvasive imaging model proposed in this work will be further studied for long-term detection and observation and may potentially be widely utilized in susceptibility testing and therapeutic effect evaluation.
This paper describes experiments made to develop a technique for marking insects and get the informations for ecological studies as a means of tagging flies with radioactive phosphorus, and also to learn the effect on the subsequent generation of flies. Tests were conducted with house flies, Musca domestica L. emerged front the larvae that had reared in a media containing various concentrations of P-32. Radioactivity of the flies was measured with end-window type $(2mg/cm^2)$ Geiger-Muller counter and proportional counter made by Aloka. In following the daily increases in counting rates by 6-day-old larvae it was apparent the larvae in the starting media containing the higher concentrations of P-32 were showing high counting rates. It was found that about 13.2 per cent of the radioactive material remained in the empty puparia after the flies emerged. Radioactivity of the emerged flies decreased gradually and the biological halflife of phosphorus-32 was approximately 6.14 days with considerable variation. A significant amount of P-32 were detected in the abdominal and thoracic sections as compared with other parts of adult. The activity of phosphorus·32 in eggs and larvae produced by adults reared in medium containing 0.2 microcurie or more of P-32 per gram were readily detected with Geiger-Mi·lier counter. On the other hand, the radioactivity of eggs and larvae produced by the treated males crossed with untreated normal females could be only detected in the medium containing more of 2.0 microcurie with G.M. counter. There was no apparent damage to the flies at dosages as high as 0.5 microcurie per gram of medium. For field studies a concentration of 0.5 microcurie of P-32 per gram of artificial medium appears to give adequate labeling for positive identification of flies and their egg masses.
The purpose of this research is to develop stereotactic localization and radiation measurement system for the efficient and precise radiosurgery. The algorithm to obtain a 3-D stereotactic coordinates of the target has been developed using a Fisher CT or angio localization. The procedure of stereotactic localization was programmed with PC computer, and consists of three steps: (1) transferring patient images into PC; (2) marking the position of target and reference points of the localizer from the patient image; (3) computing the stereotactic 3-D coordinates of target associated with position information of localizer. Coordinate transformation was quickly done on a real time base. The difference of coordinates computed from between Angio and CT localization method was within 2 mm, which could be generally accepted for the reliability of the localization system developed. We measured dose distribution in small fields of NEC 6 MVX linear accelerator using various detector; ion chamber, film, diode. Specific quantities measured include output factor, percent depth dose (PDD), tissue maximum ratio (TMR), off-axis ratio (OAR). There was small variation of measured data according to the different kinds of detectors used. The overall trends of measured beam data were similar enough to rely on our measurement. The measurement was performed with the use of hand-made spherical water phantom and film for standard arc set-up. We obtained the dose distribution as we expected. In conclusion, PC-based 3-D stereotactic localization system was developed to determine the stereotactic coordinate of the target. A convenient technique for the small field measurement was demonstrated. Those methods will be much helpful for the stereotactic radiosurgery.
The social consciousness on fake reviews has triggered researchers to suggest ways to cope with them by analyzing contents of fake reviews or finding ways to discover them by means of structural characteristics of them. This research tried to collect data from blog posts in Naver and detect habitual patterns users use unconsciously by variables extracted from blogs and blog posts by a machine learning model and wanted to use the technique in predicting fake reviews. Data analysis showed that there was a very high relationship between the number of all the posts registered in the blog of the writer of the related writing and the date when it was registered. And, it was found that, as model to detect advertising reviews, Random Forest is the most suitable. If a review is predicted to be an advertising one by the model suggested in this research, it is very likely that it is fake review, and that it violates the guidelines on investigation into markings and advertising regarding recommendation and guarantee in the Law of Marking and Advertising. The fact that, instead of using analysis of morphemes in contents of writings, this research adopts behavior analysis of the writer, and, based on such an approach, collects characteristic data of blogs and blog posts not by manual works, but by automated system, and discerns whether a certain writing is advertising or not is expected to have positive effects on improving efficiency and effectiveness in detecting fake reviews.
Today, consumers can access Internet from everywhere, therefore most commercial and other organizations provide their services on the Web. As the result, countless Web service systems are already on the Internet and more systems are under construction. Therefore, many researches of verifying that the system to be constructed will not have any deadlock and will run successfully without any problem at the early stage of design have been performed. Several Petri net based verification methods have also been published. However, they have focused on building Petri net models of Web service systems and none of them introduces efficient analysis methods. As a mathematical technique with which we can find the minimum duration time needed to fire all the transitions at least once and coming back to the initial marking in a timed net, the minimum cycle time method has been widely used in computer system analysis. A timed net is a modified version of a Petri net where a transition is associated with a delay time. A delay time used in a timed net is a constant even though the duration time associated with an event in the real world is a stochastic number in general. Therefore, this paper proposes 'Mixedly Distributed Stochastic Timed Net' where a transition can be associated with a stochastic number and introduce a minimum cycle time analysis method for 'Mixedly Distributed Stochastic Timed Net'. We also introduce a method of analysing a Web service system's response time with the minimum cycle time analysis method for 'Mixedly Distributed Stochastic Timed Net.'.
As a mathematical technique with which we can find the minimum duration time needed to fire all the transitions at least once and coming back to the initial marking in a timed net, the minimum cycle time method has been widely used in computer system analysis. A timed net is a modified version of a Petri net where a transition is associated with a delay time. A delay time used in a timed net is a constant even though the duration time associated with an event in the world is a stochastic number in general. In the consequence, the result of minimum cycle time analysis is not realistic. Therefore, we propose ‘Stochastic Timed Net' where a transition can be associated with a stochastic number and introduce a minimum cycle time analysis method for ‘Stochastic Timed Net’ As an example of the application of ‘Stochastic Timed Net’, we introduce a ‘Stochastic Timed Net' model of a Location Based Service Providing Multimedia System and the result of minimum cycle time analysis of it. Whereas the typical form of the result of the existing minimum cycle time analysis is 'It takes at least 10 time units', the typical form of the result of minimum cycle time analysis of a ‘Stochastic Timed Net' is in the probability form such as "The probability of the events in which it finishes its job within 10 time units is 85%."
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