• Title/Summary/Keyword: marine soils

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카라기난 분해효소 생산균의 분리, 동정 및 효소생산 최적 조건

  • Yang, Sung-Tack;Joo, Dong-Sik;Park, Jung-Je;Lee, Jung-Suck;Kim, Myung-Sik;Lee, Eung-Ho
    • Microbiology and Biotechnology Letters
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    • v.24 no.6
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    • pp.652-656
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    • 1996
  • The 80 strains which produce carrageenan degrading enzyme were isolated from soils, mud, seaweed, marine moluscus and echonodermata samples. Among them, one isolated strain, which showed the highest activity to produce carrageenan degrading enzyme, was used for this study. The isolated strain was identified as Pseudomonas alcaligenes through its morphological, biochemical, and physiological characteristics. The best conditions for enzyme production were 0.7% nutrient broth and 0.2% carrageenan as nitrogen and carbon source, respectively. The optimal pH, NaCl, temperature and culture time for carrageenan degrading enzyme were 7.0, 1.5%, 30* and 96hrs, respectively.

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Comparison of CPTU and DMT Results on Inchon International Airport Marine Soils (인천국제공항 부지 해성 세립토에 대한 CPTU와 DMT 결과 비교)

  • 김주형;김영웅;조성민;김명모
    • Journal of the Korean Geotechnical Society
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    • v.16 no.6
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    • pp.23-33
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    • 2000
  • 인천국제 공항에 분포하는 해성세립토 지반에서 일련의 CPTU와 DMT를 실시하고 그결과로부터 구한 비배수전단강도($s_{u}$ ), 과입밀도(OCR), 압밀계수($c_{h}$) 등과 같은 지반 정수와 기존의 시험자료 등을 분석하여 두 시험 결과를 비교하였다. 이 분석에의하면, CPTU와 DMT 두시험은 모두 피에조콘 정수, $B_{q}$ )로 분류하여 점성토인 지반에서 보다 신뢰성이 높은결과를 주었으며, 또한 해석방법에 따라 지반정수 산정결과에 큰 편차를 보여, 적절한 해석방법의 선택이 신뢰성있는 지반정수의 산정을 위한 주요한 인자임을 알았다. 그리고, 소성적 성질이 낮은 세립토 지반에서의 CPTU와 DMT를 이용한 비배수전단 강도 또한 과압밀비의 산정은 상당한 오차를 유발할 수 있다고 판단되었다.

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Behavior Analysis of the Saemangum Waterproof Embankment Applying Geotextile Tube Method and Example of Field Test - In Concentration of Reviewing the Construction and Design Process - (지오텍스타일 튜브공법을 적용한 새만금방수제의 거동분석 및 시험시공 사례 - 설계과정 및 시공성 검토 중심으로 -)

  • Park, Jong Gun;Ko, Jeong Hee;Park, Tae Seup
    • Journal of The Korean Society of Agricultural Engineers
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    • v.55 no.6
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    • pp.155-165
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    • 2013
  • Recently, geotextile tube method can be widely applied to the river, costal and marine in the construction field, such as embankment, groin, breakwater, dyke structures and so on, in advanced countries of the world. And that has been constructed at the temporary road for incheon, ilsan-bridge construction and coast erosion protection in republic of korea. Geotextile tube is a tube shaped geotextile product and hydraulic pumping filled with dredged soils. In this paper, the numerical analysis was performed to investigate the behavior of geotextile tube with various properties of material character, shape condition, construction pressure and so on. Also, the field test was conducted in order to identify the construction ability of Samangum waterproof embankment using geotextile tube. According to the applied of field test, geotextile tube was 65 m long and 4.0 m diameter. Also, the permeability coefficient and ultimate tensile strength of geotextile tube is $1.6{\times}10^{-1}$ cm/sec and 205.26 kN/m, respectively. As a result of filed test, when filled, geotextile tube does not attain the same as its unfilled theoretical diameter, but may reach approximately of 55 percent of the theoretical diameter. At the time, geotextile tubes were 12.56 m in circumference and filled to a height of about 2.2 m. This paper presents case study on field application and behavior analysis of the saemangum waterproof embankment donggin 1 division construction using geotextile tube.

Liming Materials and Desalinization of Marine Originated Tidal Soil (석회(石灰)의 종류(種類)와 해성간척지(海成干拓地) 토양(土壤)의 제염(除鹽))

  • Oh, Wang-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.107-113
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    • 1990
  • In comparision with calcium sulphate, the effect of calcium-carbonate, -silicate and -hydroxide on desalinization of tidal saline soil was investigated in a continuous leaching column experiments after mixing with an equivalent amount of Ca to sodium plus magnessium in the saline soil. One half of liming materials was mixed to the top one-tenth of column soil and the remainder was spread on the surface. Results obtained are as follows ; 1. Gypsum made easy to percolate and desaline (Na) tidal marine soil but accumulated magnessium in subsoil. 2. $Ca(OH)_2$, $CaCO_3$, and $CaSO_3$ precipitated Mg in the soil which limes were mixed, but they washed down magnessium more severely from the immediate bellow the limed soil and less from the subsequent soil layers. This leaching was more severer at the treatment of $Ca(OH)_2$and lowest at the treatment of $CaSiO_3$. 3. The alkalinity of lime in addition to the dissociation of exchangeable Na raised pH of limed leached tidal soil and slowed down the percolation rate which retarded desalining Na from limed saline soils. This effect was most severe in the $Ca(OH)_2$ treated soil. 4. pH of leached soils was correlated possitively with exchangeable Na and negatively with exchangeable Mg giving follwing relationship pH= 7.77+0.489 Na/Mg r = 0.845.

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Genesis and Mineralogical Characteristics of Acid Sulfate Soil in Gimhae Plain -I. Transformation of Pyrite and Jarosite (김해평야(金海平野)에 분포(分布)한 특이산성토(特異酸性土)의 생성(生成)과 광물학적(鑛物學的) 특성(特性) -I. Pyrite와 Jarosite의 생성(生成))

  • Jung, Pil-Kyun;Yoo, Sun-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.26 no.3
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    • pp.204-214
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    • 1993
  • The purpose of this study was to elucidate the chemical changes and formation of sulfur minerals following reduction and subsequent oxidation of the acid sulfate soils derived from the fluvio-marine plains in Gimhae area. Changes in pH, Eh and water soluble $SO_4$, Fe, Al, K, na and Ca were determined in the soil under the reduced and oxidized conditions. These chemical properties were related to the formation of the pyrite and jarosite, the major sulfur minerals in the acid sulfate soils. On incubation, suspension pH tended to increase with decreaseing Eh in the reduction periods. Jarosite formation was favored by maintaining continuous low pH(below 4.0) and high Eh(above 400mV) during the oxidation periods, however, the conditions were not favorable for the soils with $Ca(OH)_2$. Water soluble K increased by reduction but decreased by oxidation, while the jarosite of the soil with $Ca(OH)_2$ was dissolved even under the oxidation conditon, resulting in rapid increase of water soluble K. The water soluble Ca decreased rapidly, indicating that gypsum was formed with $Ca(OH)_2$ during the oxidation periods. The formation of jarosite was favored by the oxidation condition, and hindered by the reduction condition. But the formation of pyrite was favored by reduction and hindered by oxidation. When the troll was treated with $Ca(OH)_2$, Jarosite was dissolved in both oxidized and reduced conditions.

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Comparative Evaluation on Geotechnical Information 3D Visualization Program for Dredging Quantity Estimation (준설 물량 산출을 위한 지반정보 3차원 가시화 프로그램 비교 평가)

  • Lee, Boyoung;Hwang, Bumsik;Kim, Han-Saem;Cho, Wanjei
    • Journal of the Korean GEO-environmental Society
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    • v.17 no.7
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    • pp.35-42
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    • 2016
  • There are many reclamation projects domestically and internationally which requires large quantity of reclaimable materials. To provide enough reclaimable soils which are limited in land, there have been various research focusing on the dredged soils in the marine environments. As a part of this research, a GIS based 3D dredging reclamation visualization program was developed for the volume estimation of dredged soils in 2015. The developed program is based on the digitized spatial information of the site investigation data with a consideration of the reliability of the data. Prior to the validation with the comparisons with the actual dredged volume measurement data, the developed program was compared with the commercial 3D visualization program with 3D visualized results from the test site near the Gunjang harbor. The validation of the developed program was performed in terms of the degree of visualized precision, the sectional and profiling of soil layers and the dredged volume estimation results. Based on the comparisons, both commercial and developed program show similar dredged volume with minor discrepancies in soil layers.

A Study on the Characteristics of Fluvio Marine Soils developed in the West South Coastal area (서남해안(西南海岸) 간석지토양(干潟地土壤)의 특성(特性)에 관(關)한 조사연구(調査硏究))

  • Shim, Jae-Hwan;Jung, Jung-Hwa;An, Yeul
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.4
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    • pp.280-284
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    • 1989
  • The soil texture and the physico-chemical characteristics of 442,000ha reclaimable tide land in the south-western of korean peninsular were analysed. The subsidence which may occur as the soil ripened was studied. The results were as follows : 1. Among the 257,000ha of existing reclaimed tidal land 53.0% was tine silty soil and 36.0% coarse silty, 6.0% coarse loamy and 5.0% sandy soils, respectively. 2. Out of the total 442,000ha of reclaimable tidal land, 51.0% was coarse silty soil, and 20% sandy, 15.0% coarse loamy and 14.0% fine silty textural family, respectively. The coarse silty deposits were mainly distributed in the Gyeong gi and Jeonnam coast, while the coarse deposits(Coarse Loamy-sandy) exist in the Jeonbuk coastal area, but in the Chungnam areas there were various textural grades. 3. Reclaimable tidal Land in the south-western part of the peninsular was Classified into saline and alkaline soil. Electric Conductivity in saturation extract was extremely high that was 46~51 mmhos/cm, E.S.P was more than 25% and pH was ranged around 7.5~8.0 4. Reclaimed to cultivated field the subsidence reclaimable tide land to be expected when was about 18% in Soil and 21% in Sicl soils calculated down to 1.25m of the profile.

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Effect of Average and Cyclic Shear Stress on Undrained Cyclic Behavior of Marine Silty Sand (해양 실트질 모래의 비배수 동적 거동에 대한 평균 및 반복전단응력의 영향)

  • Muhammad, Safdar;Son, Su-Won;Kim, Jin-Man
    • Journal of the Korean Geotechnical Society
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    • v.30 no.1
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    • pp.17-25
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    • 2014
  • Offshore wind turbine foundations are subjected to wind, current and wave loadings. Hence, both static and cyclic behaviors of foundation's soil are important for the design of offshore wind turbine foundation. Undrained cyclic behaviors of soils depend upon the number of loading cycles, vertical effective stress, cyclic shear strain, relative density, and the combination of cyclic and average shear stresses. In order to evaluate the effect of average and cyclic shear stresses on the undrained cyclic behavior of marine silty sand, cyclic direct simple shear (CDSS) tests are performed with relative density of 85%, vertical effective stress of 200 and 300 kPa, and failure criteria of either 15% double amplitude cyclic shear strain (${\gamma}_{cyc}$) or permanent shear strain (${\gamma}_{p}$). The results are presented in the form of design graphs or contour diagrams. The undrained cyclic behavior of marine silty sand is found to be dependent on cyclic and average shear stresses and/or the combination of both shear stresses. It is found that when significant average shear stress exists the permanent or progressive shear strain is the govering failure criteria instead of cyclic shear strain.

Characterization and phylogenetic analysis of halophilic bacteria isolated from rhizosphere soils of coastal plants in Dokdo islands (독도 해안식물로부터 분리된 호염성 세균들의 특성 및 계통학적 분석)

  • You, Young-Hyun;Park, Jong Myong;Lee, Myung-Chul;Kim, Jong-Guk
    • Korean Journal of Microbiology
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    • v.51 no.1
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    • pp.86-95
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    • 2015
  • To study the halobacterial diversity at the rhizospheric soil of coastal plant native to Dokdo islands, several host plant were selected and its rhizospheric soil was sampled. Soil sample was diluted serially and pure isolation was done by sub-culture using marine agar media. 26 halophilic strains cultivable at the marine medium containig concentration of 9.0% sodium chloride were selected among total 161 isolates. Their partial 16S rRNA gene sequences extracted from genomic DNA were analyzed and partially identified. Furthermore, to identify their genetic relationship, phylogenetic tree was deduced. Total 26 strains were belongs to Firmicutes (30.8%), Gamma proteobacteria (53.8%), Bacteroidetes (7.7%), Alpha proteobacteria (7.7%), and Actinobacteria (7.7%). These results showed the specific difference from previous researches which has been reported the microbial flora of soil or sea water around the Dokdo islands. Furthermore, 4 among 26 halophilic strains grew at above 12.0% NaCl concentrated marine broth, and 2 strains Idiomarina abyssalis LM4H23 and Halomonas huangheensis AS4H13 grew at 15.0% concentration. These halophilic strains thought to overcoming the severe stress like high salt concentration or variation derived from Dokdo-specific climate and might have unknown, specific relationship with their host coastal plant native to Dokdo islands.

Diversity and Phylogenetic Analysis of Culturable Marine Bacteria Isolated from Rhizosphere Soils of Suaeda japonica Makino in Suncheon Bay (순천만 칠면초의 근권으로부터 분리된 해양세균의 다양성 및 계통학적 분석)

  • You, Young-Hyun;Park, Jong Myong;Nam, Yoon-Jong;Kim, Hyun;Lee, Myung-Chul;Kim, Jong-Guk
    • Journal of Life Science
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    • v.25 no.2
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    • pp.189-196
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    • 2015
  • Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.