• 한국발생생물학회지:발생과생식
• /
• 제22권1호
• /
• pp.9-18
• /
• 2018

This study was conducted to investigate the skeletal development of bullhead torrent catfish, Liobagrus obesus larvae and to utilize them as basic data for the taxonomic study of Liobagrus larvae. Skeletal development was observed by being divided into cranium, visceral skeleton, shoulder girdle bone, pelvic girdle bone and vertebra. On the first day after hatching, the pre-larvae had an average total length of 7.92 mm, and a line-shaped parasphenoid ossified in the cranium. In the jaw bone, the dentary supporting the lower jaw and the maxillary supporting the upper jaw were ossified. In the anterior abdominal vertebrae of the vertebra, seven centrums began to ossify and five neural spines ossified simultaneously. On the 3 day after hatching, pre-larvae had an average total length of 8.95 mm, and the prefrontal ossified in cranium. The number of abdominal vertebrae was increased to 14, and three parapophysis developed from the front side. On the 24th day after hatching, post-larvae had an average total length of 15.2 mm and the epural bone ossified in coccyx. The parhypural bone was ossified, and ossification of coccyx and pelvic girdle bone was completed. On the 30th day after hatching, the average total length of the juvenile was 17.8 mm, and the ossification of cranium and visceral skeleton was all completed while the preorbital and three suborbitals were ossified in the orbital region of the cranium.

• 수산해양교육연구
• /
• 제26권2호
• /
• pp.245-256
• /
• 2014

잉어, Cyprinus carpio와 동자개, Pseudobagrus fulvidraco의 상악 하악 수염을 조직학적으로 조사하였다. 동자개의 수염은 연골성 증축(axial rod of cartilage), 신경섬유다발(bundle of nerve fiber), 표피(epidermis), 평활근 층(smooth muscle layer) 및 미뢰(taste bud)로 구성되었으며, 잉어의 수염은 표피, 신경섬유다발, 혈관(blood vessel) 및 미뢰로 구성되었다. 수염 길이에서 잉어는 상악 바깥쪽 수염(second maxillary barbel)이 상악 안쪽 수염(first maxillary barbel) 보다 길게 나타났으며, 동자개는 하악 안쪽(inner mandibular barbel), 상악 위쪽(upper maxillary barbel), 하악 바깥쪽(outer mandibular barbel), 상악 아래쪽(lower maxillary barbel) 순으로 길게 나타났다(P<0.05). 미뢰의 수를 고려하였을 때, 동자개와 잉어간의 미각에 대한 유의적 차이가 없었다(P>0.05). 아울러, 두 어종의 모든 수염에서 수염 상부의 미뢰 수가 하부의 미뢰 수 보다 높게 나타났다(P<0.05). 본 연구 결과, 동자개의 수염은 딱딱하며 굴절성인 수염(flexible and stiff type)이었으며 잉어의 수염은 연하고 유연한 수염(tender and yielding type)으로 파악되었다.

• Genomics & Informatics
• /
• 제21권4호
• /
• pp.51.1-51.5
• /
• 2023

The genus Neoarius, known as marine catfish, is a group of the family Ariidae, composed of 10 species found in Oceania. None of the species in this genus have their mitochondrial genome described, which is highly valuable in phylogenetic and molecular evolution studies. For the present work, eight species from the Neoarius genus were selected: Neoarius utarus, Neoarius midgleyi, Neoarius graeffei, Neoarius leptaspis, Neoarius berenyi, Neoarius paucus, Neoarius pectoralis, and Neoarius aff. graeffei. DNA sequences of the eight species were obtained through the NCBI Sequence Read Archive (SRA) database, and the mitochondrial genomes were assembled using the NOVOplasty tool on the Galaxy platform, subsequently annotated with the MitoAnnotator tool. We then utilized the protein-coding genes from the mitogenomes to estimate the phylogenetic relationships within the group, including seven additional mitogenomes available in the NCBI. In all species, the mitochondrial genomes presented 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 D-loop.

• 한국어류학회지
• /
• 제11권2호
• /
• pp.126-133
• /
• 1999

무지개송어 (Oncorhynchus mykiss), 메기 (Silurus asotus) 그리고 미꾸리 (Misgurnus anguillicaudatus)의 성숙 난자의 방사대 (zona radiata) 및 난문의 미세구조적 특징을 광학, 주사 및 투과 전자현미경을 이용하여 조사하였다. 무지개송어와 메기의 난에 있는 난문은 깔대기 형태의 전정과 방사대를 통과하는 끝이 작아지는 통로로 구성되어 있었다. 무지개송어와 메기의 난문은 편평한 구멍과 긴 통로로 구성된 구조를 나타내었다. 메기 난문의 구조는 무지개송어 난문의 구조와 일치되었다. 무지개송어의 난문을 구성하는 내부 벽은 시계방향(우선형)의 구조를 나타내었다. 미꾸리의 난문은 짧은 통로를 지닌 얕은 구멍을 가진 형태를 나타내었고, 시계방향의 반대방향으로 난문의 벽은 방향성율 나타내었다. 미꾸리의 이러한 나선형의 방향은 무지개송어의 방향과 일치하지 않는 것으로 나타내었다. 개구부 바로 주변부에 있는 표면은 실험에 이용된 3가지 어류에서 모두 여러 가지 크기의 작은 구멍이 존재하였다. 상호 연결부위 (ridge)가 모든 어류의 미수정된 난자에서 관찰되었다. 본 논문에서는 난성숙시 난막과 난문의 형태를 알아보기 위해서 3가지 양식어종 난자의 표면형태와 난문의 미세구조를 상호 비교하였다.

• Genomics & Informatics
• /
• 제21권3호
• /
• pp.39.1-39.15
• /
• 2023

DNA barcoding without assessing reliability and validity causes taxonomic errors of species identification, which is responsible for disruptions of their conservation and aquaculture industry. Although DNA barcoding facilitates molecular identification and phylogenetic analysis of species, its availability in clariid catfish lineage remains uncertain. In this study, DNA barcoding was developed and validated for clariid catfish. 2,970 barcode sequences from mitochondrial cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes and D-loop sequences were analyzed for 37 clariid catfish species. The highest intraspecific nearest neighbor distances were 85.47%, 98.03%, and 89.10% for COI, Cytb, and D-loop sequences, respectively. This suggests that the Cytb gene is the most appropriate for identifying clariid catfish and can serve as a standard region for DNA barcoding. A positive barcoding gap between interspecific and intraspecific sequence divergence was observed in the Cytb dataset but not in the COI and D-loop datasets. Intraspecific variation was typically less than 4.4%, whereas interspecific variation was generally more than 66.9%. However, a species complex was detected in walking catfish and significant intraspecific sequence divergence was observed in North African catfish. These findings suggest the need to focus on developing a DNA barcoding system for classifying clariid catfish properly and to validate its efficacy for a wider range of clariid catfish. With an enriched database of multiple sequences from a target species and its genus, species identification can be more accurate and biodiversity assessment of the species can be facilitated.

• 한국발생생물학회지:발생과생식
• /
• 제21권2호
• /
• pp.193-204
• /
• 2017

The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the $G_l-$, the S- and the $G_2+M-phase$ fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.

• 환경생물
• /
• 제39권1호
• /
• pp.55-67
• /
• 2021

메기(Silurus asotus)를 대상으로 210일간 기아 시 계측형질 및 체 절단면 형질을 측정하여 그 기아 효과를 조사하였다. 실험 종료시 섭이군의 생존율은 92.2±0.47%였으며, 기아군에서는 74.4±2.59%의 생존율을 보였다. Ls, DALPO/Ls, DADOP/Ls, ED/Ls, CH/Ls, BDAA/Ls, BDMA/Ls 및 BWAA/Ls의 8개 항목에서 유의한 차이를 보였으며(p<0.05), 다른 16개 항목 DALAD/Ls, DPDPL/Ls, HPLAA/Ls, HALAV/Ls, HALOP/Ls, DAUPO/Ls, DADAV/Ls, DADAA/Ls, Max BL/Ls, Man BL/Ls, LD/Ls, HWOP/Ls, DAUF/HL, DAUS/HL, IW/HL 및 DAUE/HL에서는 초기군, 섭이군 및 기아군의 세 그룹 간 유의한 차이가 없었다(p>0.05). 섭이군은 각 체 절단면에서 기아군보다 더 높은 전체 높이(THX), 더 넓은 너비(WX) 및 더 넓은 면적(AX)을 보였다. 본 연구는 메기의 기아 상태에 대한 외형 및 체 절단면 형질에 대한 정보를 제공한다. 이는 메기에 대한 먹이 조절에 대한 방법을 보조하고 영양상태를 나타내는 기본 기초자료로 활용될 수 있을 것이다. 또한, 본 연구에서 사용한 조사 분석 방법을 타 어종에서 적용 시 그 영양 상태에 대한 정보를 파악하는 데 유용하게 활용될 수 있을 것으로 사료된다.

• 한국해양바이오학회지
• /
• 제3권1호
• /
• pp.31-37
• /
• 2008

Off the 1,572 km long coastline of Namibia lies known as the Benguela upwelling system, a very rich source of marine life supporting traditional and modern forms of fishery. Commercial fishing and fish processing is one of the fastest-growing sectors of the Namibian economy in terms of employment, export earnings, and contribution to GDP. The fishing industry has grown to the extent that it is currently Namibia's second biggest export earner of foreign currency after mining (90% of national output is marketed for export). In 2005, Namibia harvested about 552,164 tonnes of fish. The final value of processed products (export value) that year was around US$ 376.0 million. Besides the marine captured fisheries, Namibia also has a small but vibrant aquaculture sector. Inland captured fisheries exist in the north-east and north-west of Namibia where as commercial freshwater aquaculture of tilapia and catfish is also undertaken. The inland fisheries are mainly subsistence based and typically labour intensive, with low catch per unit effort. However the subsistence fisheries from these regions play a significant role in the lives of rural community. The domestic market for marine fish products is extremely limited due to the small size of the population (2 million). The fishing industry is a source of considerable employment for many Namibians. Huge potential to increase production exists in Namibia, unpolluted high quality marine waters, high natural primary productivity of the seawater, availability of inexpensive fish by-products from established fish processing sector for inclusion in wet aqua-feeds and well-established processing, packaging and marketing systems due to the marine capture fisheries that can be adopted for aquaculture purpose.

• 한국어병학회지
• /
• 제22권3호
• /
• pp.343-352
• /
• 2009

Natural-killer-cell-enhancing factor (NKEF) belongs to the newly defined peroxiredoxin (Prx) family. It was originally isolated from human erythroid cells. The black rockfish NKEF cDNA was identified through the expressed sequence tag (EST) analysis of PBLs libraries. The full-length NKEF cDNA was 1433 bp long and contained an open reading frame (ORF) of 594 bp that encoded 198 amino-acid residues. The 5' UTR had a length of 39 bp, and the 3’UTR 800 bp. The deduced amino-acid sequence of the black rockfish had a density 93.4, 92.9, 87.8, 85.8, 84.8, 83.8, 80.3, 79.7, 77.2, and 75.2% that of the pufferfish, olive flounder, channel catfish, zebrafish, chicken, common carp, Myotis lucifugus, cattle, human PrxI, rat PrxI, human NKEF-A, and Xenopus tropicalis, respectively. The NKEF gene was expressed in all the tissues of the black rockfish. The RT-PCR indicated that the NKEF transcripts were predominantly in the spleen and gill, less dominantly in the PBLs, head kidney, trunk kidney, and liver, and least in the intestine and muscles. This is the first report on the existence of the NKEF-A gene in black rockfish.

• 한국어류학회지
• /
• 제13권1호
• /
• pp.85-88
• /
• 2001

메기 Silurus asotus에서 염색체조작을 효과적으로 하기 위하여, 여러 온도 조건에서의 난할율과 제2, 제3 난할 시간의 평균화에 의한 체세포분열 간격지수 (${\tau}_o$)를 조사하였다. 빠른 난 발생과 비동시적 발생은 고온에서 더욱 뚜렷하게 나타났다. 메기의 체세포분열 간격지수는 $22^{\circ}C$에서 $21.5{\pm}1.4$분, $24^{\circ}C$에서 $18.5{\pm}1.2$분 그리고 $26^{\circ}C$에서 $14.0{\pm}2.1$분이었다. 3가지 수온조건과 ${\tau}_o$ 간에는 Y=-1.85X+21.9 ($R^2=0.9868$, Y는 ${\tau}_o$ 그리고 X는 수온)의 직선회귀가 성립되었다.