• 한국수산과학회지
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• 제17권4호
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• pp.321-326
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• 1984

열대수역에 서식하는 3종류의 Mangrove 굴을 대상으로 하여 각각의 형태적 특성을 비교하였다. Venezuella산인 Crassostrea rhizophorae는 가장 둥근형태이며 각내용적(殼內容積)이 가장 컸다. 비만도도 가장 높아 $9.63\%$였다. Liberia산인 Crassostrea tulipa는 외형에서 가장 길죽한 형태이며 각내용적은 크지만 비만상태는 $8.15\%$로 중간이었다. Indonesia산인 Crassostrea belcheri는 Venezuella산과 갈이 둥근 형태이며, 전체 무게와 껍데기 무게는 가장 컸지만 비만도는 가장 낮아 $6.62\%$였다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.535-538
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• 2006

The coastline of the Choa Phraya Rivermouth in the Upper Gulf of Thailand has been changed drastically. The western side especially at Ban Laem Sing was eroded for 400 meter in 26 years or with the rate of 15 meter per year on the average. The Metropolitan Electricity Authority has granted five years research project to us to study the efficiency of the electric pole fence as a mean to reduce the wave force and increase sedimentation rate inside for mangrove replantation. If the method works efficiently, it will be used to reclaim the coastal land loss in other areas in Thailand. The project was done since 2005 after the fence was constructed completely in October 2005. The oceanographic surveys were conducted before and after the construction in northeast monsoon season to study the impact of physical oceanographic forces on the coastline change. The sedimentation rates were measured each month. It is rather low about 2 cm/month. The subsiding rate from the load of deltaic sediment and groundwater withdraw using boring data is about 30 mm/year. The cumulative sinking rate is 55.8 cm from 1978 to 1995. We have tried to design the method of mangrove plantation in this area. The remote sensing data such as LANDSAT and aerial photos from 1987 to 2002 for 15 years were used to compute the rate of coastline change at each 50 m section along the western side of the rivermouth.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2127-2137
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• 2016

A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Two-dimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple "omics" analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.

• Advances in Traditional Medicine
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• 제7권4호
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• pp.379-384
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• 2007

Two medicinal plant of Sundarbans mangrove forest has been tested for the evaluation of growth inhibitory and antibacterial activity. The methanol extract of Xylocarpus granatum stem bark showed potent wheat rootlet ($IC_{50}=0.01{\mu}g/ml$) and shoot ($IC_{50}=0.23{\mu}g/ml$) growth inhibitory activity in a concentration related manner. The growth inhibitory activity was markedly decreased in residual methanol extract. The methanol extract showed antibacterial activity (MIC > 3 mg/ml) against Bacillus subtilis, Staphylococcus aureous and Proteus vulgaris. The residual methanol extract did not show any antibacterial activity. The results suggest the bioactive principle(s) of Xylocarpus granatum may be relatively non polar compound(s). The methanol extract and residual methanol extract of Sarcolobus globosus stem showed poor wheat rootlet and shoot growth inhibitory activity and no antibacterial activity.

• Natural Product Sciences
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• 제10권1호
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• pp.11-15
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• 2004

The leaves of Rhizophora apiculata, a plant belonging to the family Rhizophoraceae were collected from the mangrove forest of Sunderbans, West Bengal, India. Alcoholic extract of the leaves of this plant was prepared and hypoglycemic/anti-hyperglycemic activity was studied in fed rats, glucose loaded rats and streptozotocin induced diabetic rats. The results of this study reveal that this plant extract has potential hypoglycemic action.

• Mycobiology
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• 제43권2호
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• pp.137-149
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• 2015

In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration ($LC_{50}$) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1034-1043
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• 2014

This study determined the effect of light intensity and photoperiod on the dry cell weight and total amount of carotenoids in four isolates of purple non-sulfur bacteria obtained from shaded and exposed microhabitats of a mangrove ecosystem in Kota Kinabalu, Sabah, Malaysia. The initial isolation of the bacteria was carried out using synthetic 112 medium under anaerobic conditions (2.5 klx) at $30{\pm}2^{\circ}C$. On the basis of colony appearance, cell morphology, gram staining, motility test, and 16S rRNA gene sequencing analyses, all four bacteria were identified as Afifella marina. One of the bacterial isolates, designated as Af. marina strain ME, which was extracted from an exposed mud habitat within the mangrove ecosystem, showed the highest yield in dry cell weight ($4.32{\pm}0.03g/l$) as well as total carotenoids ($0.783{\pm}0.002mg/g$ dry cell weight). These values were significantly higher than those for dry cell weight ($3.77{\pm}0.02g/l$) and total carotenoid content ($0.706{\pm}0.008mg/g$) produced by the isolates from shaded habitats. Further analysis of the effect of 10 levels of light intensity on the growth characteristics of Af. marina strain ME showed that the optimum production of dry cell weight and total carotenoids was achieved at different light intensities and incubation periods. The bacterium produced the highest dry cell weight of 4.98 g/l at 3 klx in 72 h incubation, but the carotenoid production of 0.783 mg/g was achieved at 2.5 klx in 48 h incubation. Subsequent analysis of the effect of photoperiod on the production of dry cell weight and total carotenoids at optimum light intensities (3 and 2.5 klx, respectively) revealed that 18 and 24 h were the optimum photoperiods for the production of dry cell weight and total carotenoids, respectively. The unique growth characteristics of the Af. marina strain ME can be exploited for biotechnology applications.

• 한국산학기술학회논문지
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• 제14권6호
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• pp.3114-3119
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• 2013

망그로브 생태계는 수생태계로 유입되는 중금속을 받아들이는 기능을 가지고 있다. 중금속에 오염된 퇴적물에 노출됨에도 불구하고 망그로브는 중금속에 내성을 가지고 있다. 이 연구에서 우리는 망그로브로부터 중금속 저항성 관련 유전자를 클로닝하고, 중금속 노출에 유전자 발현 변화를 분석하였다. 미크로네시아 축라군의 웨노섬에서 채취한 Rhizophora stylosa의 잎과 뿌리조직으로부터 CTAB 방법을 이용하여 RNA를 분리하였고, gene specific primers를 이용하여 phytochelatin synthase 1(PCS1) 유전자를 클로닝하였다. R. stylosa 태생종자를 100 ppb의 Cd과 10 ppb의 Cu에 노출하였을 때 각각 1.91배 및 2.72배 발현이 증가하였다. 이러한 결과는 PCS1 유전자의 발현분석이 망그로브 생태계의 건강성을 평가하기 위한 좋은 도구가 될 수 있음을 나타낸다.

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.280-291
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• 2021

The study examined the influence of climate and soil dynamics on vegetation health across the ecological zones in Rivers State, Nigeria. MODIS imagery was used to assess the vegetation health through NDVI and point grid pattern of meteorological data for total precipitation (TP), air temperature (AT), soil moisture (SM) and soil temperature (ST) of 2000, 2003, 2006, 2009, 2012 and 2015 were used for the study. Descriptive and inferential statistics were used for data analysis. Findings showed that NDVI ranged between 0.420 and 0.612 in the freshwater swamp (FWS) while between 0.465 and 0.611 in the rainforest and the NDVI in the mangrove was generally low. The highest mean AT was experienced in the mangrove ecological zone and the least was experienced in the rainforest. The mean SM was generally highest in the rainforest with highest value in 2000 (774.44 m³/m³). The ST was highest in the mangrove and the least was experienced in the rainforest while the TP was highest in the mangrove. NDVI correlated significantly with SM (r=0.720; p<0.05) and ST (r= -0.493; p<0.05). NDVI, SM, TP and ST significantly varied among the ecological zones. Regression analysis showed that vegetation health was significantly related to the combination of soil temperature and soil moisture (R²=0.641; p=0.000). Thus, monitoring the factors that affect vegetation health in a changing climate and soil environments is highly required.

• Journal of Microbiology and Biotechnology
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• 제19권7호
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• pp.675-680
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• 2009

There is considerable interest in the isolation of potent radical scavenging compounds from natural resources to treat diseases involving oxidative stress. In this report, four new fungal metabolites including one new bisdihydroanthracenone derivative (1, eurorubrin), two new seco-anthraquinone derivatives [3, 2-O-methyl-9-dehydroxyeurotinone and 4, 2-O-methyl-4-O-(${\alpha}$-D-ribofuranosyl)-9-dehydroxyeurotinone], and one new anthraquinone glycoside [6,3-O-(${\alpha}$-D-ribofuranosyl)-questin], were isolated and identified from Eurotium rubrum, an endophytic fungal strain that was isolated from the inner tissue of the stem of the marine mangrove plant Hibiscus tiliaceus. In addition, three known compounds including asperflavin (2), 2-0-methyleurotinone (5), and questin (7) were also isolated and identified. Their structures were elucidated on the basis of spectroscopic analysis. All of the isolated compounds were evaluated for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity.