• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.8
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• pp.1206-1212
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• 2010

This study was performed to establish optimum extraction condition of Tricholoma matsutake. A central composite design was applied to investigate the effects of independent variables, extraction temperature ($X_1$), extraction time ($X_2$) and water per sample ($X_3$) on dependent variables such as soluble solids contents ($Y_1$), total phenolics contents ($Y_2$), reducing sugar contents ($Y_3$), electron donating ability ($Y_4$) and nitrite scavenging ability ($Y_5$). The optimum extraction conditions were predicted and monitored by response surface methodology using SAS program based regression analysis. Soluble solids content, electron donating ability and nitrite scavenging ability were highly affected by water per sample. However, the contents of total phenolics and reducing sugar were affected by water per sample and extraction temperature as well. The optimum extraction conditions for soluble solids were 34.84 mL/g (water/sample) at $78.85^{\circ}C$, for 3.33 hr. In contrast, the optimum extraction conditions of electron donating ability were temperature of $91.00^{\circ}C$, time of 1.62 hr and water per sample of 39.42 mL/g. Taken together, the optimum ranges for hot water extraction of Tricholoma matsutake were $70{\sim}90^{\circ}C$, 2~4 hr and 30~50 mL/g.

• (The)Study of the Eastern Classic
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• no.62
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• pp.145-176
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• 2016

This paper aims to define the leadership of Gunja (君子, translated into prince, gentleman, or ideal man) based on Confucian Classics which offer the general values and norms of individuals' virtue and social virtuous acts. Thus, humanitarianism is regarded as true value, and the values of a virtuous person who properly practices social human relationships are discussed. The real worth of Gunja image is discussed as a true human image of "self-completion and completion of all things" (成己成物) which involves the convergence of truth, good and beauty where there is a sense of harmony and balance, where there is stern self discipline and self cultivation and where win-win values of human relationships are created. Confucian saint (聖人), wise man (賢人), great man (大人), and gentleman (君子) mean social leaders. They practice human morals, enlighten and beautify society with teachings, and are indicated as equipped with mental and material harmony, good character and competence, and economic power and morality. People today pursue their own personal growth according to their material preferences rather than pure intellectual cultural values, and are engrossed in visually beautiful external unlimited competition. In this digital age, we are supposed to demonstrate our individuality, but many people are obsessed with appearance, go on severe diet, and lose their health beauty, and consequently suffer mental stress. This trend fuels obsession with appearance and the sick practice of valuing appearance. As an alternative method to overcome this phenomenon, we need a leader image with the convergence of truth, good and beauty, which is characterized by internal self cultivation, external professionalism, and handsome and solid character. Confucian thoughts consist in practicing the Way of disciplining oneself for governing others (修己治人). Self discipline involves developing personal virtuous ability for cultivating a virtuous character, and governing others involves interacting to work together in society and to have right human relationships. Thus, leaders should impress not only themselves but also others. Self discipline for governing others means cultivating virtue for oneself and leading others. A true leader has self introspection and establishes himself through self discipline so that he can govern others or reach the realm of settling others where people live together. As all things have a value and a virtue, humans endeavor to cultivate character and virtue by learning and studying for securing their professionalism, reliability, character and ability, so as to create their own brand value. Personal character does not come from a high position, wealth and power. Character is a personal virtue, and is cultivated as immaculate and fresh through self discipline. As such, it well matches with a clean and clear spirit. This offers the ideal leader as the Guja image who has an extremely humane character, as well as being equipped with inherent virtues of intellect, benevolence and courage. Self development can foster virtue and self management through self leadership and self discipline. The leader in the relationship area can practice his virtue through virtuous acts, in other words, even think from another person's perspective. Such leader is mentioned as the principle of measuring square in the Great Learning. In our viewpoint, the beauty of character can breed the seed of virtue through intellect, benevolence and courage, the beauty of win-win can realize the right virtue by showing exemplary acts to others through considerateness, and the beauty of harmony can love and care for others like me through the principle of measuring square, thereby realizing the universal principle of virtue and harmony, which is like my mind. As such, the ideal leader, when his virtue and mind of being considerate of others all blending well, can exercise his ability to the full, can live together and coexist with many people, and can grow again into a triumphant relationship.

• Journal of Korean Academy of Nursing
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• v.4 no.2
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• pp.32-43
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• 1974

A steady increase in the aged population poses a great challenge to nurses with their diversities of health care needs, especially of psychological nature, such as an alienation problems among the aged. The purpose of this study is to examine the nature of alienation problems among the aged in urban areas to provide basic informations for a better understanding of aged people. In this study, the investigator has tried to identify causes of alienation in the aged, and to determine the extent of alienation in relation to sex, family structure, religion, economic status and participation in social activities. The survey was done during the period from the last part of August to the end of September 1973. After a day's training, 10 interviewers have interviewed 210 stratified random samples of people over the age of 65 residing in Seoul with the free-ended questionnaire prepared by the investigator. The result of the study was tested by Critical Ratio. Following Hypothesis have guides formulation of the study: I. The extent of alienation in old man would be higher than in old woman, II. The extent of alienation in the aged who live alone would be higher than those living with their family. III. The extent of alienation in the aged who have no spouse would be higher than who live with their spouse. IV. The extent of alienation in the people without religious beliefs would be higher than the people with religious biles. V. The extent of alienation in the aged would be higher in proportion to decreased level of their economic productivity. Ⅵ. The extent of alienation in the aged who do not participate in social activities would be higher than that of the aged who actively participate. The analytic results of the study are as fellows: 1. The extent of alienation in woman was revealed higher than that of man by showing significant difference at P〈0.01 level. (CR=3.66) Accordingly hypothesis I was denied. 2. The extent of alienation in the aged wile live alone was revealed higher than that of the aged who live with their family by showing a significant difference at P<0.01 level. (CR=7.31) So hypothesis II was supported. 3. The extent of alienation of the aged who have no spouse was revealed higher than that of the aged who live with their spouse at the significant level of P <0.01. CR=4.65) Accordingly hypothesis III was supported, 4. There was no significant difference in the extent of alienation between the people with and-without religion. Thus hypothesis IV was rejected. 5. The extent of alienation in the aged was found to be higher in proportion to decreased level of their economic ability. a. The aged with greatest income showed least alienation b. The aged with no income had a greater extent of alienation than the aged with minimal income by showing significant difference at P <0.01 level . (CR=4.82) c. The difference between the greatest income group and the minimal income group was. found to be less significant than the difference between minimal income group and the-people without income. Thus hypothesis V was positively supported. 6. The extent of alienation in the aged who do not participate in social activities was higher than that of the aged who actively participate. (P <0.01, CR=6.24) According1y hypothesis Ⅵ was supported.

• Molecules and Cells
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• v.38 no.7
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• pp.663-668
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• 2015

hBMSCs are multipotent cells that are useful for tissue regeneration to treat degenerative diseases and others for their differentiation ability into chondrocytes, osteoblasts, adipocytes, hepatocytes and neuronal cells. In this study, biodegradable elastic hydrogels consisting of hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(${\varepsilon}$-caprolactone) (PCL) scaffolds were evaluated for tissue engineering because of its biocompatibility and the ability to control the release of bioactive peptides. The primary cultured cells from human bone marrow are confirmed as hBMSC by immunohistochemical analysis. Mesenchymal stem cell markers (collagen type I, fibronectin, CD54, $integrin1{\beta}$, and Hu protein) were shown to be positive, while hematopoietic stem cell markers (CD14 and CD45) were shown to be negative. Three different hydrogel scaffolds with different block compositions (PEG:PCL=6:14 and 14:6 by weight) were fabricated using the salt leaching method. The hBMSCs were expanded, seeded on the scaffolds, and cultured up to 8 days under static conditions in Iscove's Modified Dulbecco's Media (IMDM). The growth of MSCs cultured on the hydrogel with PEG/PCL= 6/14 was faster than that of the others. In addition, the morphology of MSCs seemed to be normal and no cytotoxicity was found. The coating of the vascular endothelial growth factor (VEGF) containing scaffold with Matrigel slowed down the release of VEGF in vitro and promoted the angiogenesis when transplanted into BALB/c nude mice. These results suggest that hBMSCs can be supported by a biode gradable hydrogel scaffold for effective cell growth, and enhance the angiogenesis by Matrigel coating.

• Journal of Sasang Constitutional Medicine
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• v.4 no.1
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• pp.107-148
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• 1992

The definitions of Four Constitution (Tae-Yang-In, So-Yang-In, Tae-Eum-In, So-Eum-In) in Four-Beginning-Theory (四端論) and Distribution-Theory (擴充論) were morphologically diagrammatized. the method of measuring the five parts came from the diagrams. The five parts are the followings; Top-Line is the distance between Rt. & Lt.Shoulders. Bosom-Line is the horizontal distance passing through the Rt. & Lt.nipples. Stomach-Line is the horizontal distance passing through the Rt. & Lt. acupuncture point Bool-Yong (不容穴). Navel-Line is the horizontal distance passing through the navel. Bottom-Line is the distance between the Rt.& Lt. anterior superior iliac spines. According to it, 311 people were measured in the clinic. Through the measured numerical values, to find out the objective propriety of the morphological diagrammings and the discriminating ability of Four Constitutions by the method of measuring the five, the author tried this study and got the following results. 1. On the ground of the Internal Organs-Theory (臟腑論), the definitions of Four Constitutions were classified and diagrammatized step by step and it was possible to draw the morphological graphs satisfying the reasonable condition objectively. 2. It was found that the graphings by Mean, Standard Deviation and 95% Confidence Interval for mean of the numerical values of five measured parts and the morphological diagrammings of the definitions in Four-Beginning Theory and Distribution-Theory drove to the same figures. 3. Through the method of the Analysis of variance and the Discriminant Analysis, it was discovered that the measured numerical values of five parts could distinguish the Four Constitutions. 4. Tae-Yang-In has the longest Top-Line and the shortest Bottom-Line. The Mean head of Confidence Interval from Top to Bottom is downwardly decreased at the rate of 3.7, 1.5, 1.8, 3.4. 5. So-Yang-In has the longest Top-Line and the shortest Bottom-Line. The Mean head of confidence Interval from Top to Bottom is downwardly decreased at the rate of 2.3, 1.5, 1.5, 1.1. 6. Tae-Eum-In has the longest Navel-Line. The Mean head of Confidence Interval from Navel to Top is upwardly decreased at the rate of 0.5, 0.4, 2.5 and downwardly decreased at the rate of 2.7 between Navel and Bottom. 7. So-Eum-In has the longest Bottom-Line. The Mean head of Confidence Interval from Bottom to Top is upwardly decreased at the rate of 1.1, 1.1, 1.8, 2.3. From the above findings, it was possible to draw the morphological graphings satisfying the objectively reasonable conditions in the definitions of Four-Beginning-Theory and Distribution-Theory and it was proved that the results of the Positive Analysis, carried with the measured numerical values of five parts from the diagramming, could show the objective propriety of the morphological graphings and the discerning ability of Four Constitutions by the method of measuring the five parts.

• Food Science of Animal Resources
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• v.35 no.6
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• pp.765-771
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• 2015

The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B₁₂ content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B₁₂ was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B₁₂ detection. The calibration curve showed good linearity with the coefficient of correlation (r²) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B₁₂ recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B₁₂ content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B₁₂. This method could be implemented in laboratories that require time and labor saving.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1071-1077
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• 2017

A rise in the occurrence of allergic diseases is attributed to the dysregulated balance of type 1/type 2 immunity, where type 2 T-helper (Th2) cells predominate over type 1 T-helper (Th1) cells, leading to an abnormally increased production of IgE in response to unharmful antigens. Kimchi, a traditional Korean fermented food, is a rich source of beneficial lactic acid bacteria. In this study, we investigated the ability of Enterococcus faecium FC-K derived from kimchi to induce type I immunity in the presence of Th2 polarizing conditions in vitro and in vivo. Stimulation of mouse peritoneal macrophages with E. faecium FC-K induced the production of tumor necrosis factor alpha, interleukin (IL)-6, and IL-12. Under the in vitro Th2 conditions in which splenic T cells were activated in the presence of IL-4, E. faecium FC-K enhanced the ability of T cells to produce interferon $(IFN)-{\gamma}$. Using the ovalbumin (OVA)-induced allergy model, male BALB/c mice receiving E. faecium FC-K reduced the serum level of total IgE, but not that of OVA-specific IgE. Furthermore, the population of activated splenic B cells during OVA immunization was decreased in E. faecium FC-K-treated mice, accounting for a reduction of total IgE in the serum. Restimulating splenocytes from OVA-immunized mice with OVA ex vivo resulted in an increased production of $IFN-{\gamma}$, with no effect on IL-4, in E. faecium FC-K-treated mice. These observations provide the evidence that E. faecium FC-K can be a beneficial probiotic strain that can modulate the Th2-mediated pathologic response.

• Food Science and Preservation
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• v.22 no.2
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• pp.267-274
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• 2015

This study was conducted to evaluate the antioxidant activities and the quality characteristics of 70% ethanol extracts from onion peels with different extraction methods (autoclave extraction, AE; low temperature high pressure extraction, LTPE; reflux extraction, RE; and stirrer extraction, SE). The yields of AE, LTPE, RE and SE were 9.00%, 5.39%, 13.21% and 12.41%, respectively. The total polyphenol and flavonoid contents in the AE were significantly higher than in the other extracts. The DPPH radical scavenging abilities with a concentration of 100 mg% (w/v) were : AE, 28.9%; RE, 26.07%; LTPE, 24.35%; and SE, 19.53%. The ABTS radical scavenging ability and the nitrite scavenging activity showed the same tendency as that of the DPPH radical scavenging ability. The angiotensin-converting enzyme (ACE) inhibitory activities of the LTPE and AE were higher than those of the RE and SE. The ${\alpha}$-Glucosidase inhibitory activity of the RE was higher than that of the extracts with other extraction methods. The nitrite scavenging activities with a concentration of 10 mg/mL were: AE. 33.97%; RE, 35.47%; LTPE, 21.86%; and SE, 21.71%. The ferrous ion chelating activity of the LTPE (54.73%) was significantly higher than that of the other extracts. These results suggest that AE is the superior method for the enhancement of anti-oxidant activity, and onion peel can be used as a natural antioxidant material for health foods and can be a good ingredient of functional foods.

• The Journal of Pediatrics of Korean Medicine
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• v.19 no.2
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• pp.175-195
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• 2005

Objective: This experimental study was performed to examine the in vitro and in viva anti-inflammatory and anti-allergic effects of Mori Cortex. Methods: Water extract of Mori Cortex was studied to its ability to stimulate or inhibit macrophage 264.7 cells to produce inflammatory and allergic mediators. Cytokines such as $IL-1{\beta}$, IL-6, IL-10 and $TNF-{\alpha}$ were measured by immunochemical assay. In vitro, the macrophages 264.7 were classified into four groups. One group was a normal group. The other group was a (-) control group stimulated with LPS. And the third group was a (+) control group pretreated for 1 hour with hydrocortisone. And the fourth group was a sample group pretreated for 1 hour with Mori Cortex. After pretreatment, macrophage were incubated with lipopolysaccharide(LPS) $100\;ng/m{\ell}$ for 12 hour and media collected and $IL-1{\beta}$, IL-6, IL-10 and $TNF-{\alpha}$ concentrations in supernatants were measured each by Enzyme linked immuno-soubent assay. Mori Cortex were used $50\;{\mu}g/m{\ell},\;100\;{\mu}g/m{\ell},\;250\;{\mu}g/m{\ell},\;500\;{\mu}g/m{\ell},\;and\;1,000\;{\mu}g/m{\ell}$. Hydrocortisones were used $10^{-8}M,\;10^{-7}M,\;10^{-6}M,\;10^{-5}M\;and\;10^{-4}M$. In vivo, the SD rats were classified into three groups. One group was a normal group injected with normal saline into the abdominal cavity. The other was a control group prescribed to compound 48/80 after normal saline injection. And the third was a sample group prescribed to compound 40/80 after Mori Cortex injection. Then, the release of histamine, IL-6 and $TNF-{\alpha}$ were measured. Results : In vitro, Man Cortex significantly increased the release of $IL-1{\beta}\;and\;TNF-{\alpha}$ by LPS-stimulated macrophage 264.7 cells. And it significantly decreased the release of IL-10. In IL-6, Mori Cortex of low concentration significantly decreased the release of IL-6, but that of high concentration acted in reverse. In vivo, Man Cortex didn't show significant inhibitory effects on the release of histamine and IL-6 in comparison with that of the control group. But it significantly increased the release of $TNF-{\alpha}$ in comparison with that of the control group.

• Journal of the Korean Geographical Society
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• v.32 no.1
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• pp.113-124
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• 1997

The purpose of this article is to analyze some problems of the present curriculum of social studies education and develop a newer hierarchical curriculum system for the 7th Curriculum Reformation in the primary and secondary school of Korea. Most of the major problems of social studies education come from the mixture of different subject matters without reasonable education logic. Most of teachers feel some difficulties in teaching the social studies for the lack of other subject knowledges. For the better teaching, it is needed to control the integrating level according to the learner's intellectual ability from the elementary school to high school. So, it is necessary to enhance students' thinking skills by their own ability of restructuring knowledges from all subject matters in more advanced curriculum. Also, it is required to develop a new hierarchical system of curriculum concerning the geographical view of the world. Students can have many kinds of geographical view points if teachers can well organize learning activities logically for the students to enhance their understanding of geography by adjusting scale of regional unit, geographical theme, and resolution level of interpretation. And then, in the elementary school curriculum, the students are required to understand the spatial variation of places, and various environments in the world, scale. In the junior high school, students have to understand the relationship between man and nature in the context of large scale environments. The high school curriculum needs to be focused on the student's understanding of the spatial contexts of places by theoretical application in the smaller scale region different from elementary and junior high school curriculums.