• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.628-635
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• 2001

Recombinant E. coli DH5$\alpha$ harboring a dextransucrase gene (dsrB742) produced an extracellular dextransucrase in a 2% sucrose medium. The enzyme was purified by DEAE-Sepharose and Phenyl-Sepharose column chromatographies upto a 142.97-fold purification with a 11.11% recovery to near homogeneity. The enzyme had a calculated molecular mass of 168.6 kDa, which was in good agreement with the activity band of 170 kDa on a nondenaturing SDS-PAGE. An expression plasmid was constructed by inserting the dsrB742 into a pRSET expression vector. The activity after expression in E. coli BL21(DE3)pLysS increased about 6.7-fold compared to the extracellular dextransucrase from L. mesenteroides B-742CB. The expressed and purified enzyme from the clone showed similar biochemical properties (acceptor reaction, size of active dextransucrase, optimum pH, and temperature) to B-742CB dextransucrase, however, the ability to synthesize ${\alpha}$-(1$\rightarrow$3) branching decreased in comparison to that of L. mesenteroides B-742CB dextransucrase.

• PNF and Movement
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• 제7권4호
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• pp.37-43
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• 2009

Purpose : The purpose of this study was to evaluate the effect of core stability training at deep abdominal muscle for balance control of hemiplegic patient. Method : The subject of this study was a 47-year-old man with right hemiplegia. He was treated five times a week for three weeks with core stability training at deep abdominal muscles. Evaluation tool was used Functional reach test(FRT), timed up and go test(TUG) and one leg standing for stroke patients. Result : The FRT distance increase, TUG time decrease, one leg standing time increase core stability training at deep abdominal muscles for right hemiplegia improved was the ability for maintain balance. Posture and control of trunk stability are changing posture, and so which showed significant improve of total balance control. Conclusion : The result of this study showed that core stability training at deep abdominal muscles is an effective treatment for balance control. Therefore, it could be considered as a treatment method in the rehabilitation of stroke patient with poor postural control and imbalance, although further studies are needed.

• 한국CDE학회논문집
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• 제1권1호
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• pp.33-44
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• 1996

This study deals with the development of wire path searching module as a part of automobile wire harness design system. Wire path searching module manages the free space, finds transition locations, and creates bundle paths to dramatically reduce a tedious iterative routing process which results in easy optimization of the bundle paths. A prime policy in the system configuration is to compromise between man's and computer's ability, and make it possible a designer's leading role in designing process. Human input is indispensable to cope with the special cases which were not considered in the initial design stage of the system. In this study, we improve the previous shortest-path-finding algorithm, (VGraph and RCA method) into a new method called Extended RCA. Bundles, connectors and transitions are handled as objects so one can manage and modify physical properties of the objects easily. Therefore a verification is allowed at any desired stage of design. The reuse of previous result is facilitated by using Dependency Structure, which represents the mutual relations among connectors, transitions, and bundles. Dependency Structure makes it possible the elimination of redundant calculating process, and consequently shorter routing time.

• 시큐리티연구
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• 제13호
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• pp.305-326
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• 2007

21세기 사회에 진입하면서 시민의 안전한 삶을 위협하는 범죄와 테러같은 위험요인들이 증가하고 있다. 따라서 개인의 생명, 신체, 재산 등의 아전의 욕구는 증가 할 수 밖에 없다. 국가중요시설은 적의공격으로부터 파괴되거나 기능이 마비가 될 경우에 국가적으로 중대한 영향을 미치는 시설을 말하며, 특수경비원들이 담당하고 있는 국가중요시설은 적 또는 불순분자, 테러리스트들의 제일의 공격목표가 될 수 밖에 없기 때문에 우리나라에서는 경비가 강화외도 있는 실정이다. 본 연구의 특수경비제도의 현황과 문제점을 검토해보고 발전방안을 모색해보는데 연구의 목적을 두고 있다. 특수경비제도의 발전은 한국 민간경비산업의 발전으로 이어지기 때문에 특수경비제도의 발전을 위해서는 이 분야에 근무하는 경비근무자들의 근무환경개선, 유능한 자원의 확보, 보수의 현실화, 전문자격증제도의 검색시스템의 개선을 행함으로써 한국민간경비산업의 발전에 가속도를 붙일 수 있을 것이다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.251.2-251.2
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• 2013

Natural photosynthesis utilizes two proteins, photosystem I and photosystem II, to efficiently oxidize water and reduce NADP+ to NADPH. Artificial photosynthesis which mimics this process achieve water splitting through a two-step Z-schematic water splitting process using man-made synthetic materials for hydrogen fuel production. In this study, Z-scheme system was achieved from the hybrid materials which composed of hydrogen production part as photosystem I protein and water oxidizing part as semiconductor BiVO4. Utilizing photosystem I as the hydrogen evolving part overcomes the problems of existing hydrogen evolving p-type semiconductors such as water instability, expensive cost, few available choices and poor red light (>600 nm) absorbance. Some problems of photosystem II, oxygen evolving part of natural photosynthesis, such as demanding isolation process and D1 photo-damage can also be solved by utilizing BiVO4 as the oxygen evolving part. Preceding research has not suggested any protein-inorganic-hybrid Z-scheme composed of both materials from natural photosynthesis and artificial photosynthesis. In this study, to realize this Z-schematic electron transfer, diffusion step of electron carrier, which usually degrades natural photosynthesis efficiency, was eliminated. Instead, BiVO4 and Pt-photosystem I were all linked together by the mediator gold. Synthesized all-solid-state hybrid materials show enhanced hydrogen evolution ability directly from water when illuminated with visible light.

• Physical Therapy Rehabilitation Science
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• 제11권4호
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• pp.471-477
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• 2022

Objective: The greatest motor impairment after stroke is a decreased ability to walk. Most stroke patients achieve independent gait, but approximately 70% do not reach normal speed, making it difficult to reach a standard of daily living. Therefore, a wearable exoskeleton is recommended for optimal independent gait because different residual disorders hinder motor function after stroke. This review synthesized the effect on gait speed in randomized controlled trials (RCTs) in which gait training using a wearable exoskeleton was performed on post-stroke patients for qualitative and quantitative analysis. Design: A systematic review and meta-analysis of a randomized controlled trials Methods: RCTs using wearable exoskeletons in robotic rehabilitation of post-stroke patients were extracted from an international electronic database. For quality assessment and quantitative analysis, RevMan 5.4 was used. Quantitative analysis was calculated as the standardized mean difference (SMD) and presented as a random effect model. Results: Five studies involving 197 post-stroke patients were included in this review. As a result of the analysis using a random effect model, gait training using a wearable exoskeleton in post-stroke patients showed a significant improvement in gait speed compared to the non-wearing exoskeleton (SMD=1.15, 95% confidence interval: 0.52 to 1.78). Conclusions: This study concluded that a wearable exoskeleton was more effective than conventional gait training in improving the gait speed in post-stroke patients.

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.1869-1874
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• 2010

By reducing PVP with $H_2NOH$.HCl and NaOH 2:2:1 mass ratios in aqueous ethanol, poly-N-vinyl pyrrolidone oxime [PVPO] was prepared with 92% yield. Applying the sol-gel concept, orthosilicic acid [OSA] was made by hydrolyzing TEOS with ethanol in 1:0.5 molar ratios using 1 N KOH aqueous solution as a catalyst. The OSA + PVPO + $_L$-Valine ($\alpha$-amino acid) were mixed with pure ethanolic medium in 1:2:2 mass ratios and refluxed at $78^{\circ}C$ and 6 pH for 6.5 h. A white residue of poly-N-vinyl pyrrolidone oximo-L-valyl-siliconate [POVS] appeared after 5 h. The heating of reaction mixture was stopped and the contents were brought to NTP. The residue formation of POVS was intensified with lowering a temperature and completely solidified within 5 h, was filtered using a vacuum pump with Whatmann filter paper no. 42. The residue of POVS was washed several times with 20% aqueous cold ethanolic solution and dried in vacuum chamber at $25^{\circ}C$ for 24 h. The MP was noted above $350^{\circ}C$. Structural and internal morphology were analyzed with IR and $^1H$-NMR, and SEM respectively. A drug loading and transporting ability of the POVS in water and at pH = 5 and 8 was determined chromatographically.

• 한국미생물·생명공학회지
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• 제16권4호
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• pp.316-319
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• 1988

토양으로부터 분리한 알카리내성 Bacillus sp. YA-14의 Pectate lyase(PL) 유전자를 E. coli에 cloning하여 제조한 재조합 plasmid pYPC29는 삽입 된 1.6kb 단편내에 PL 유전자를 함유하고 있었으며, 이 외래 DNA가 Bacillus sp. YA-14의 chromosomal DNA에서 유래된 것임을 Southern hybridization을 통하여 확인하였다. 재조합 plasmid pYPC29는 E. coli내에서 안정하게 존재하였으며 이를 함유한 재조합체의 전체 PL 활성 중 약 70%가 periplasmic space에 존재하였다.

• Nuclear Engineering and Technology
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• 제47권5호
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• pp.546-558
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• 2015

Chord length sampling method in Monte Carlo simulations is a method used to model spherical particles with random sampling technique in a stochastic media. It has received attention due to the high calculation efficiency as well as user convenience; however, a technical issue regarding boundary effect has been noted. In this study, after analyzing the distribution characteristics of spherical particles using an explicit method, an alternative chord length sampling method is proposed. In addition, for modeling in finite media, a correction method of the boundary effect is proposed. Using the proposed method, sample probability distributions and relative errors were estimated and compared with those calculated by the explicit method. The results show that the reconstruction ability and modeling accuracy of the particle probability distribution with the proposed method were considerably high. Also, from the local packing fraction results, the proposed method can successfully solve the boundary effect problem. It is expected that the proposed method can contribute to the increasing of the modeling accuracy in stochastic media.

• 방사성폐기물학회지
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• 제19권1호
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• pp.39-49
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• 2021

The behaviors of various desorption agents were investigated during the desorption of cesium (Cs) from samples of clay minerals and actual soil. Results showed that polymeric cation exchange agents (polyethyleneimine (PEI)) efficiently desorbed Cs from expandable montmorillonite, whereas acidic desorption solutions containing HCl or PEI removed considerable Cs from hydrobiotite. However, most desorption agents could desorb only 54% of Cs from illite because of Cs's specific adsorption to selective adsorption sites. Cs desorption from an actual soil sample containing Cs-selective clay mineral illite (< 200 ㎛) and extracted from near South Korea's Kori Nuclear Power Plant was also investigated. Considerable adsorbed 137Cs was expected to be located at Cs-selective sites when the 137Cs loading was much lower than the sample's cation exchange capacity. At this low 137Cs loading, the total Cs amount desorbed by repeated washing varied by desorption agent in the order HCl > PEI > NH4+, and the highest Cs desorption amount achieved using HCl was 83%. Unlike other desorption agents with only cation exchange capabilities, HCl can attack minerals and induce dissolution of metallic elements. HCl's ability to both alter minerals and induce H+/Cs+ ion exchange is expected to promote Cs desorption from actual soil samples.