• Title/Summary/Keyword: malt

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Production of Inulin Fructotransferase(depolymerizing) from Flavobacterium sp. LC-413

  • Cho, Chul-Man;Lim, Young-Soon;Kang, Soo-Kyung;Jang, Kyung-Lib;Lee, Tae-Ho
    • Preventive Nutrition and Food Science
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    • v.1 no.1
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    • pp.121-126
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    • 1996
  • A bacterial strain LC-413, producing extracellular inulin fructotransferase which converts inulin into di-D-fructofuranose dianhydride(DFAIII) and amount of oilgosaccharides, was isolated from soil and pre-sumed as Flavobacteium sp. LC-413. The enzyme production was induced by inulin as carbon source and enhanced by the addition of 0.3% malt extract and 0.2% {TEX}$NaNO_{3}${/TEX} as nitrogen source. The enzyme activity in the culture supernatant reached at the maximum, 78.6units/ml, after 11 hours of cultivation in the medium composition of 1.5% inulin, 0.2% {TEX}$NaNO_{3}${/TEX}, 0.05% {TEX}$K_{2}${/TEX}{TEX}$HPO_{4}${/TEX}, 0.05% {TEX}$MgSO_{4}${/TEX}.7{TEX}$H_{2}${/TEX}O, 0.05% KCI, a trace amount of {TEX}$FeSO_{4}${/TEX}.7{TEX}$H_{2}${/TEX}O, and 0.3% malt ext. at 3$0^{\circ}C$. The oilgosaccharide produced by enzyme reaction from inulin was identified as DFA III by and {TEX}${13}^C${/TEX}-NMR spectrosocpy.

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Antioxidant Activity of Indigenous Trametes Species in Korea

  • Kim, Minkyeong;Ahn, Chorong;Kim, Changmu
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.433-440
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    • 2021
  • The fungal genus Trametes is globally distributed and comprises various wood-decay species, including the well-known medicinal mushroom Trametes versicolor, a popular remedy in traditional Asian medicine. Trametes species produce antioxidants, which have a wide range of health benefits. The pressent study evaluated seven indigenous Trametes species from Korea, which were cultivated in three different media (dextrose-yeast extract, DY; malt extract-yeast extract, MY; malt extract broth, MEB) and tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. We found that the medium consumption rate did not significantly differ between the media and among the strains (72-76%). However, the T. versicolor strains had a relatively low consumption rate (14-65%). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) tests demonstrated that culture filtrates of T. cf. junipericola, T. orientalis, T. suaveolens, and T. versicolor possessed antioxidant activity against damage from free radicals. In particular, T. cf. junipericola (DY) and T. versicolor (MY) had >80% activity in the DPPH and ABTS assays, compared with that of the positive control (ascorbic acid). Thus, our study identified promising candidates with substantial antioxidant activity among the indigenous strains of Trametes spp. from Korea.

A Case of Mucosa-Associated Lymphoid Tissue Lymphoma (MALT Lymphoma) in Hypopharynx: A Case Report (하인두에 발생한 점막 연관성 림프조직 림프종 1예: 증례 보고)

  • Min-Ju Kang;Youn-Sun Park;Ju-Eun Lee;Hyun-Bum Kim
    • Korean Journal of Head & Neck Oncology
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    • v.39 no.1
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    • pp.33-36
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    • 2023
  • Malignant lymphoma rarely occurs in the larynx and hypopharynx. Few cases of malignant lymphoma in the larynx were reported in Korea. However, malignant lymphoma in the hypopharynx had been not reported in Korea. A 68-year-old woman came to the outpatient clinic with a foreign body sensation in her throat. A round, smooth margin, bright pink-colored mass was confirmed by the laryngoscopy. The patient took neck computer tomography. A small bulging of mucosa was observed, but there was no peripheral infiltration or abnormally enlarged lymph nodes. We did excision using CO2 LASER. She was finally diagnosed with mucosa-associated lymphoid tissue lymphoma (MALT lymphoma). After diagnosis, several image studies confirmed that there was no metastatic lesion. She got only radiotherapy after that and kept a complete response state for over 2 years.

Quality Characteristics of Syrup made with Saccharified Barley Liquid (보리당화액을 첨가한 시럽의 제조와 품질특성)

  • Kim, Ji-Hyun;Cho, Eun-Ju
    • Culinary science and hospitality research
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    • v.21 no.6
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    • pp.242-254
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    • 2015
  • Barley is a main food source, along with rice, in our dietary life that is easy to buy and process. It is required to develop a beverage base and barley syrup for desserts in order to raise utilization of barley beyond its present use in Sikhye (sweet fermented rice drink) and Jocheong (grain syrup) production. In pursuit of the goal to increase the usability of barley as an ingredient of processed food, this study examined the optimal preparation conditions of barley mash for barley syrup. In addition, the study prepared a barley syrup using saccharified barley liquid, analyzed quality characteristics, and conducted a sensory evaluation. Saccharified barley liquid(barley mash) was prepared with ratios of adding malt of unhulled barley germinated as 0 g(CON), 10 g(BM10), 20 g(BM20), and 30 g(BM30). The results of measuring pH under the varied conditions of saccharification temperatures from $50^{\circ}C$, $60^{\circ}C$, and $70^{\circ}C$, as well as time from 1 to 8 hours, treveal that the optimum condition for malt saccharification is $60^{\circ}C$ for 5 hours. By adding oligosaccharides and lemon juice to the saccharified barley liquid at the different ratios of added malt, this study measured quality characteristics(color value, viscosity, pH, sweetness) of barley syrup warmed up for 1 hour. As the result of measuring color in the barley syrup prepared by saccharified barley liquid, higher levels of added malt saw L-value decreased while a-value and b-value both increased. BMs30 showed the highest viscosity of $1,202.67{\pm}3.06$. As for pH, BMs30 was the highest at pH $3.57{\pm}0.02$. The result of the sensory evaluation of barley syrup showed the superior sensory characteristics of BMs20 in terms of color, flavor, sweetness, viscosity and overall quality.

Studies on the Response of Isogenic lines of Compact and lax Type to Fertilization and Plant Density in Malting Barley (맥주맥에 있어서 직두형 및 수두형 Isogenic line의 시비량 및 재식밀도에 대한 반응)

  • Chang-Hwan Cho;Eun-Sup Lee;Je-Cheon Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.3
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    • pp.366-374
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    • 1986
  • Taiwan land race, Taichung 2 rowed barley #1 was crossed with England cultivar, Deba abed in 1972, and two isogenic lines-lax and compact heads-were made in 1982. So as to three fertilizer and three seeding density levels were treated at Dan-Kook Agricultural College farm located in Cheonan in 1984-1985. Lax head type had longer spike length and rachis than compact type. The traits uninfluenced by different head types were heading time, number of grains per spike, yield and soluble nitrogen content. However, the traits related to malting quality such as 1,000 grain weight, assortment ratio, protein content of grain and malt, malt extract, malt total nitrogen content, Kolbach index and diastatic power were significantly affected by head types. The number of spikes per m2, yield, assortment ratio, crude protein content of grain and malt yield ratio, extract yield ratio, malt total nitrogen, souble nitrogen content, Kolbach index and diastatic power were affected by fertilizer and seeding density levels in both isogenic lines. The malting quality was best at 12-12-9 kg/l0a fertilizer level and the yield was greatest at 15-12-9 kg/l0a level. Narrow-spaced or drilling seeding had greater yield and better malting quality than conventional row seeding. The plant with lax head type had some advantages in malting quality, suggesting that this trait would be considered as an selection criterion fer good malting quality lines.

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Primary Orbital Lymphoma : A Retrospective Analysis of Results of Radiation Therapy (원발성 안와 림프종의 방사선치료 성적에 관한 후향적 분석)

  • Kim Sussan;Ahn Seung Do;Chang Hyesook;Kim Kyoung Ju;Lee Sang-wook;Choi Eun Kyung;Kim Jong Hoon;Huh Jooryung;Suh Cheol Won;Kim Sung Bae
    • Radiation Oncology Journal
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    • v.20 no.2
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    • pp.139-146
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    • 2002
  • Purpose : This study evaluated the treatment outcomes, patterns of failure, and treatment related complications of primary lymphoma patients who received definitive radiation therapy. Materials and Methods : A retrospective analysis was undertaken for 31 patients with primary orbital lymphoma at the Asan Medical Center between February 1991 and April 2001. There were 18 males and 13 females with ages ranging from 3 to 73 years (median, 44 years). The involved sites were 9 conjunctivae, 12 eyelids and 10 other orbits. The histological types were 28 MALT lymphomas (low-grade B-cell lymphoma of mucosa-associated lymphoid tissue type), 1 diffuse large B-cell lymphoma, 1 anaplastic large cell lymphoma and 1 lymphoblastic lymphoma. The Ann Arbor stages were all IE $(100\%)$. Ann Arbor stage III or IV patients were excluded from this study, Bilateral orbital involvement occurred in 6 cases. Radiation therapy was given with one anterior port of high energy electrons $(6\~16\;MeV)$ for the lesions located at the anterior structures like the conjunctivae or eyelids. Lesions with a posterior extension or other orbital lesions were treated with 4 or 6 MeV photons with appropriately arranged portals. In particular, lens blocks composed of lead alloy were used in conjunctival or eyelid lesions. Twelve patients received chemotherapy. The median follow-up period was 53 months. Results : The 5-year overall, cause-specific, and disease-free survival was $91\%,\;96\%,\;and\;80\%$, respectively. The complete response rate 6 months after radiation therapy was $100\%$. Local recurrences were observed in 2 patients at 16 and 18 months after completion of radiation treatment. They were salvaged with additional radiation therapy. Two patients developed distant metastases. A MALT lymphoma patient with a lung relapse was successfully salvaged with radiotherapy, but the other lymphoblastic lymphoma patient with bone marrow relapse expired. There were no severe complications but 5 patients developed radiation-induced cataracts and 2 patients developed dry eye. Conclusion : Most primary orbital lymphomas consisted of MALT lymphomas. Radiation therapy was a successful treatment modality for orbital lymphoma without any severe complications. In cases of local relapses, radiation therapy is also a very successful salvage treatment modality.

Cultural Condition for the Mycelial Growth of Phellinus igniarius on Chemically Defined Medium and Grains (화학합성배지 및 곡물을 이용한 Phellinus igniarius의 균사체 배양조건)

  • Jung, In-Chang;Kim, Seon-Hee;Kwon, Yong-Il;Kim, So-Yeun;Lee, Jong-Suk;Park, Shin;Park, Kyung-Sook;Lee, Jae-Sung
    • The Korean Journal of Mycology
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    • v.25 no.2 s.81
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    • pp.133-142
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    • 1997
  • The chemical media composition and culture conditions were optimized for mycelial growth of Phellinus igniarius 26005. The method of solid-state fermentation, cultivation of basidiomycetal strains in various grains, was developed. Media composition for optimal growth of Phellinus igniarius 26005 was made of 7.0% malt extract, 0.3% bacto soytone, and 0.2% yeast extract. The optimum condition for mycelial growth was $28^{\circ}C$ and pH 7.0, respectively. For the mass cultivation of mycelia, the hydrated grains with cold water, were put into the plastic bottle. The mycelial growth rate in the bottled grains was high in the early stage with inoculation of homogenized mycelium. The activity of mycelium was maintained by adding sterilized water in the middle of cultivation. The glucosamine content which determins the mycelial growth rate in solid material was in the order of job's tears>barley>black soybean>wheat>malt soybean>brown rice>sorghum>glutinous rice.

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Cultural characteristics of unrecorded species Lentinula spp. in Korea (국내 미기록 표고속 종들(Lentinula spp.)의 배양적 특성)

  • Kim, Kwang-Sang;Kim, Gyeong-Je;Kim, Hyun-Seok;Jin, Seong-Woo;Kim, Jin-Kyoung;Ban, Seung-Eon
    • Journal of Mushroom
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    • v.7 no.4
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    • pp.193-199
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    • 2009
  • The culture condition of unrecorded species L. aciculospora, L. boryana and L. raphanica was investigated with recorded species of L. edodes as the control group in order to analyze diversity and examed relations of the species belong to Lentinula. The optimal temperature and media for the mycelial growth of L. aciculospora and L. boryana were $22^{\circ}C$ and PDA, MCM medium. L. raphanica was $28^{\circ}C$ and ME1 medium. Each of L. aciculospora, L. boryana, L. raphanica is pH 6, pH 7, pH 5 in the optimal pH respectvely. The optimal carbon and nitrogen source of L. aciculospora were glucose and malt extract. That of L. boryana was glucose and urea that of L. raphanica was sucrose and potassium nitrate. The optimal vitamin of L. aciculospora was Myo-inositol. That of L. boryana and L. raphanica were Riboflabin.

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Physiological and Ecological Studies on Mycelia of Armillariella mellea (뽕나무 버섯 균사체의 생리.생태학적 연구)

  • Choi, Mi-Ja;Lee, Ji-Yul
    • The Korean Journal of Mycology
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    • v.11 no.2
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    • pp.79-84
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    • 1983
  • To study physiological and ecological characters of the secondary mycelia of Armillariella mellea, it was cultivated on the various media. It was grown very well on the malt extract medium compared with its growth on yeast extract medium, potato dextrose medium and Hamada medium. The temperature of $27^{\circ}C$ gave the best condition for it to grow. The highest rate of growth was shown in medium of pH 6. Heteroauxin of 5 ppm concentration showed a increase as 26.2% in growth in compared with the control group. Gibberelline 10ppm, Tomatoton of 10,000 dilution and Adoton of 5,000 dilution showed positive effect. It was shown that the use of above hormones in appropriate concentration brought the effect of growth, but overuse of them brought inhibitory effect. Under the same condition of 200ppm concentration, vitamin A gave the highest growth of 18.2% than that of the control used.

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Quality Characters and Identification of Breeding Materials in Malting Barley (맥주보리 품질 관련 형질과 고품질 육종소재 탐색)

  • 오세관;현종내;서득용;서세정
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.3
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    • pp.184-190
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    • 2003
  • Germplasms and breeding lines of malting barley at National Yeongnam Agricultural Experiment Station (NYAES) were evaluated for malting barley quality improvement. Among the numerous malting barley quality parameters, the mean of protein content was 12.3% between 10.7% to 14.0% and range of the $\beta$-glucan content was from 3.5% to 5.8%, the mean was 4.6 percent. The length of acrospire, non-germination rate, friability, the speed of filtration, extract yield, kolbach index, diastatic power were significantly different between the individual varieties, however the other traits were not significantly different. The results of correlation analysis among 15 quality parameters showed significant positive correlation between crude protein content and malt protein content. However, other quality parameters such as sugar content, fiablity, extract yield, and kolbach index had negative correlation with crude protein content. Therefore, crude protein content could be one of the major factors that deteriorate quality. The varieties of Viva, Nishino-chikara, Kinukei 9, Kinukei 12, Sacheon 6 and Jinyangbori that showed over the 80% in extract yield and the higher diastatic power, will be used by crossing parents for improve the quality of malting barley.