• Applied Biological Chemistry
• /
• 제31권3호
• /
• pp.241-248
• /
• 1988

서양종(西洋種) 꿀벌(Apis mellifera L)에 대(對)한 살충제의 독성(毒性) 및 해독능력(解毒能力)을 조사(調査)하고 농약한계사용량(農藥限界使用量) 결정(決定)에 기여(寄與)하기 위하여 7가지 대표적(代表的)인 살충제의 꿀벌에 대한 독성(毒性)및 해독효소(解毒酵素)의 활성(活性)을 조사(調査)하였다. 효소활성(酵素活性)은 해독효소(解毒酵素)로 알려진 microsomal oxidases, glutathione S-transferases, esterases 와 DDT-dehydrochlorinase를 조사(調査)했고 성충(成？)일별의 중장(中腸)을 사용(使用)하여 측정(測定)하였다. $LC_{50}$치(値)의 측정결과(測定決果) 공시(供試) 살충제중(殺？劑中) DDT가 58ppm으로 독성(毒性)이 가장 낮았고 EPN이 1.61ppm으로 독성(毒性)이 가장 강(强)했다. 준치사농도(準致死濃度)의 농약(農藥)이 성충(成？)일벌의 microsomal oxidase에 미치는 영향은 malathion 및 permethrin 처리(處理)가 aldrin epoxidase 활성(活性)을 저해(沮害)시켰고 N-demethylase 활성(活性)은 diazinon 처리구(處理區)에서 증대(增大)되었다. Ghtathione S-transferarse(DCNB conjugation) 활성(活性)은 diazinon과 permethrin 처리구(處理區)에서 증대(增大)되었다. Esterase는 malathion 및 permethrin 처리구(處理區)에서 ${\alpha}-NA$ esterase활성(活性)의 저해(沮害)를 보였고 diazinon처리구(處理區)에서 carboxylesterase활성(活性)이 증대(增大)되었으며 AChE 활성(活性)은 거의 영향(影響)이 없었다. DDT-dehy drochlorinase활성(活性)은 carbaryl 및 permethrin 처리구(處理區)에서 증대(增大)되었다.

• 분석과학
• /
• 제31권1호
• /
• pp.39-46
• /
• 2018

온라인 고체상 추출을 이용한 LC/MS/MS를 사용하여 carbaryl과 유기인계 농약 7 종을 동시에 분석하는 방법을 확립하였다. 분석 대상농약은 carbaryl, diazinon, methyl-demeton, fenitrothion, malathion, parathion, phenthoate, EPN 등 8 종이다. 본 연구에서는 온라인 고체상 추출과 LC/MS/MS의 ESI positive mode로 전처리 없이 물 시료 $500{\mu}L$를 직접 주입하여 10분안에 분석하였다. 농약 8 종의 회수율은 86.8~100 % 였고, 정확도는 90.6~98.8 %로 90 %이상이었고, 정밀도는 1.3~5.2 %로 나타났으며 검출한계 및 정량한계는 각각 $0.05{\sim}0.28{\mu}g/L$, $0.16{\sim}0.89{\mu}g/L$ 였다. $0.5{\mu}g/L$에서 $8.0{\mu}g/L$까지 다섯 개의 농도로 검량선을 작성한 결과 모든 물질의 상관계수(r2)가 0.99이상을 나타냈다. 방법의 유효성 평가를 한 결과 모든 내용에서 적합하므로확립한 분석방법으로 수질 시료에서 농약을 조사한 결과 모두 검출되지 않았다.

• 한국응용곤충학회지
• /
• 제35권4호
• /
• pp.297-301
• /
• 1996

온도가 살충제의 저항성과 감수성계통, 복숭아혹진덧물의 발육에 미치는 영향을 조사하기 위하여 핵형이 정상인 감수성 계통(URY-O)과 저항성 계통(ABURABI : 유일) 및 핵형이상(AI, 3 전좌)으로 저항성인 O-RY 계통을 사용하였다. $25{\circ}C$에서의 약충 기간은 저항성과 감수성 계통 간에 차이가 없었으나, $30{\circ}C$에서는 감수성 인 URY-O 계통은 8.3일간에 성층이 되었었음에 비하여 저항성인 O-RY 계통은 조사기간인 20일까지 약충상태로서 성충으로 발육하지 못하고 사망하였다. 감수성인 URY-O 계통과 저항성인 ABURABI 계통의 산자수는 $28^{\circ}C\;와\;25^{\circ}C$에서 서로 차이가 없었으나, 저항성인 O-RY 계통의 산자 수는 $28^{\circ}C\;에서\;25^{\circ}C$에서보다 1/10밖에 자충을 낳지 못하였다. 또한 충체중은 $28^{\circ}C$에서 URY-O와 유일은 각각 0.22와 0.27 mg/♀이었으나, O-RY는 0.16 mg/♀로서 차이가 컸다. 가수분해활성(n mol/IS min/female)이 19인 O-RY(-)9t 88인 O-RY(+)는 $28^{\circ}C에서\;G_1$, sub-strain의 산자수는 각각 3.4마리와 0.8마리로서 에스테라제 활성이 높은 O-RY(+)계통이 에스테라제 활성이 낮은 O-RY(-)계통보다 새끼수를 적게 낳았다.

• 대한화학회지
• /
• 제39권12호
• /
• pp.902-909
• /
• 1995

Ion trap 질량분석기와 Quadrupole 질량분석기를 이용한 밀가루 중 유기인제(dimethoate, diazi-non, parathion-methyl, fenithion, malathion) 및 인삼 중 BHC 이성질체들의 정성 정량 분석에 대한 유용성을 평가하기 위해 검출한계를 구하고, 검량선을 작성하였다. 이온화 방법으로 EI와 CI를 사용하여 mass fragmentation pattern을 비교하여 정성분석에 적용하였으며, scan과 selected ion monitoring(SIM) mode로 미량분석법으로서의 유용성을 평가하였다. 검출한계는 ion trap에서 scan시 0.008-0.225ng이었으며, quadru-pole에서는 scan시 0.23-3.1ng, SIM 측정시 0.032-0.68ng으로ion trap이 quadrupole의 scan시 보다 2-300배, SIM보다 4-30배까지 검출한계가 낮았다. 검량선의 직선성은 ion trap과 quadrupole모두 0.9이상이었다. 밀가루 추출물은 Bio-Bead S-X3를 사용하여 정제하였고, 인삼은 Florisil column으로 정제하였다. 5종의 유기인제의 회수율은 80% 이상이었으며, BHCs의 회수율은 60-90%이었다.

• 한국식품위생안전성학회지
• /
• 제37권1호
• /
• pp.1-8
• /
• 2022

본 연구는 2021년 2월부터 7월까지 잡곡 106건을 수거하여 잔류농약 실태조사를 하였다. 대형, 중소형 및 온라인 유통매장을 중심으로 국내 잡곡 40건과 수입 잡곡 66건을 수거하였고, GC/MSMS, GC/ECD, GC/NPD, LC/MSMS, UPLC/PDA, HPLC/FLD를 이용하여 다종농약 다성분분석법으로 잔류농약 341종을 분석하였다. 잔류농약이 검출된 잡곡은 대형유통매장 1건, 중소형 유통매장 2건, 온라인 유통매장 5건으로 총 8건(7.5%)이었고, 5건(4.7%)에서는 농약잔류허용기준을 초과하였다. 이들 잡곡에서는 MGK-264, chlorpyrifos, thiamethoxam, malathion, piperonyl butoxide, pirimiphos-methyl 등 6종의 농약 성분이 검출되었다. 검출된 잡곡은 강낭콩(1건), 녹두(6건), 수수(1건)이다. 검출된 잡곡 중 농약잔류허용기준을 초과한 품목은 수입 녹두(5건)로 미얀마산이었고, 초과한 농약 성분은 thiamethoxam이었다.

• Journal of Microbiology and Biotechnology
• /
• 제12권4호
• /
• pp.706-709
• /
• 2002

Bacterial bioluminescence has been known to be a highly valuable reporter system for its potential application as an effective and simple environmental monitoring method for toxic compounds. In this short report, we constructed a streptomycetes-Escherichia coli shuttle vector-containing bioluminescence system and evaluated its potential application for toxic compounds monitoring. The luxAB biolurninescence genes from Vibrio harveyi were cloned into a streptornycetes-E. coli shuttle vector (named pESK004) and functionally expressed in Streptomyces lividans. The recombinant S. lividans containing pESK004 exhibited an optimal biolurninescence at the optical density ($OD_{600\;nm}$) of 0.4-0.5 and aldehyde concentration of 0.005%. When the recombinant bioluminescence streptomycetes was exposed to a toxic compound such as heavy metals, chlorinated phenols, or pesticides, the bioluminescence was decreased proportionally to the concentration of toxic compound in the assay mixture. The $EC_{50}$ (effective concentration to decrease 50% of the bioluminescence prior to exposure) values in the recombinant biolurninescence streptomycetes for mercury, 2,4-dichlorophenol, and malathion were measured at 2.2 ppm, 144.0 ppm, and 82.4 ppm, respectively. The degree of sensitivity and specificity pattern toward these toxic compounds characterized in this recombinant bioluminescence streptomycetes were unique when compared with previously reported bacterial bioluminescence systems, and this revealed that a recombinant bioluminescence streptomycetes might provide an alternative or complementary system for potential environmental monitoring.

• Applied Biological Chemistry
• /
• 제41권4호
• /
• pp.213-217
• /
• 1998

An assay for cytochrome P450 monooxygenase activity by determination of the products of organophosphate oxidation via inhibition of acetylcholinesterase was described. Extracts from strains of Oryzaephilus surinamensis selected for resistance to chlorpyrifos-methyl (QVOS 102), fenitrothion (VOS F) and malathion (VOS 3), and a standard susceptible strain VOS 48, were incubated with an organophosphate in the presence of a NADPH-generating system and acetylcholinesterase. The degree of inhibition of the acetylchoinesterase activity was converted to manooxygenase activity using standard curves for the inhibition of acetylcholiesterase by chlorpyrifos-methyl-oxon, fenitrooxon and malaoxan. Activity was detectable in VOS 48 and was present at different increased levels with the different organophosphates in the three resistant strains, suggesting that different forms of P450 might be involved in organophosphate oxidation in these insects. The assays were carried out using crude insect homogenates and much smaller samples of insect material than the standard aldrin to dieldrin assay. It should be possible to use the method for determination of monooxygenase activity in single insert.

• 한국환경보건학회지
• /
• 제10권1호
• /
• pp.47-54
• /
• 1984

The toxicity value of 13 kinds of public health insecticides against the adult female cockroach, Periplaneta fuliginosa, Setville in Korea were evaluated by the topical application method of insecticides. The results obtained were summarized as follows 1. S-biolline showed the most strong susceptibility with LD$_{50}$ of 1.9${\mu}g$ per cockroach (1,100 mg) out of 13 kinds of insecticides and other synthetic pyrethroides insecticides (bio-resmethrin 2.8${\mu}g$/cockroach, permethrin 4.3 ${\mu}g$/cockroach) showed some degree of susceptibility to the smoky brown cockroach. 2. Fenitrothion showed 2.5 $\mu$g per cockroach which was the most strong susceptibility out of 8 kinds of organophosphorus and most of them were good susceptibile but there was clear evidence for developed resistance against malathion with LD$_{50}$ of 80.5 {$\mu}g$ per cockroach (1,100 mg). 3. Lindane showed some degree of susceptibility with LD$_{50}$ of 13.9 {$\mu}g$ per cockroach (1,100 mg) but DDT showed the most strong resistance to the smoky brown cockroach out of 13 kinds of insecticides tested.

• 한국동물학회지
• /
• 제3권1호
• /
• pp.9-12
• /
• 1960

The present status of insecticide resistance of Korean mosquitoes is still unknown. The experiment cited below is a part of our series of resistance tests on Korean mosquitoes. The test was carried out during the summer of 1959 to ascertain the degree of resistance of Culex pipiens to insecticides currently used. Culex pipiens eggs were collected from ditches in Seoul City and reared in laboratory with media consisted of the following : Distrilled water 1.0 liter, Magnesium sulfate (MgSO$_4$.7$H_2O$) 1.0gram , Calcium sulfate (CasO$_4$.2$H_2O$) 0.5gram ; Sodium cholride 0.5 gram. A Sufficient amount of beer yeast was also used as a larvar food. Late third and early fourth instar larvae were selected for testing. The results are summarized in the following. THe LC-50 of Culex pipiens to insecticides were 0.061 ppm in p, p-DDT , 0.045 ppm in ${\gamma}$-BHC and0.029 ppm in malathion. Comparing these with LC-50 doses of Orlando laboratory strain, it can be pointed out that the present resistance of Seoul strain shows about a ten-fold increase in DDt, and a fourfold increase in ${\gamma}$-BHC . The resistance development of the Seoul strain has, however, not arisen so markedly as compared with data observed in other parts of Far Eastern areas received heavy insecticidal operations.

• 한국물환경학회지
• /
• 제21권2호
• /
• pp.205-210
• /
• 2005

There has been a growing interest in NF membrane for drinking water treatment, because it can remove simultaneously both hardness and hazardous micro pollutants such as pesticides and THM precursors. In this work, a hollow fiber NF membrane known as a composite membrane was employed for the rejection properties of both hydrophilic solutes and micro organic pollutants, where the former was used to evaluate the molecular sieving effect of this membrane and the latter was employed for the investigation of solute-membrane interaction as hydrophobic materials. This membrane effectively rejected the hydrophilic solutes and the permeation of them was mainly controlled by the molecular sieving effects such as molecular weight and molecular width. In the case of all micro organic pollutants, the rejections were varied from 42.2% for Simazine to 91.6% for Malathion, and the parameters related to the steric hindrance could significantly play an important role in the rejection. In the batch type adsorption experiments, all micro organic pollutants were entrapped mildly on the membrane in spite of lower degree compared with that of aromatic compounds, and they were correlated with log K.