• Title/Summary/Keyword: malacology

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Ultrastructure of the neuromuscular Junction in the Mantle Edge of the African Giant Snail, Achatina fulica (아프리카 왕달팽이(Achatina fulica) 외투막내 신경근연접의 미세구조)

  • 장남섭;한종민;황선종
    • The Korean Journal of Malacology
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    • v.14 no.2
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    • pp.75-83
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    • 1998
  • 아프리키 왕달팽이(Achatina fulica) 외투막(mantle edge) 내 근육 속의 신경근연접에 관한 미세구조 연구결과는 다음과 같았다. 신경근연접종말의 형태는 방추형이거나 불규칙형이었으며, 연접막과 근형질막상이 연접간극(synaptic cleft)의 넓이는 30mm 정도로 나타났다. 연접강속에는 전자밀도가 높은 둥근 과립(직경 100mm)과 중등도는 과립(직경 70mm)그리고 전자밀도가 낮은 소포(직경70mm)등 3종류가 관찰되었으며, 연접종말의 외측에는 0.2 - 0.4$\mu\textrm{m}$ 정도크기의 전자밀도가 높은 둥근 과립들이 존재하였다. 전자밀도가 중등도인 둥근 과립 중 일부는 연접막 밖으로 배출(exocytosis) 되었으며, 근형질막과 연접막이 합체되는 현상도 관찰되었다. 근형질(sarcoplasm)내에서 수축성단백질인 액틴과 미오신섬유의 배열은 불규칙하였으며, 세포질성 치밀체(cytoplasmic dense body)도 관찰되었다.

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Bioconcentration of Polycyclic Aromatic Hydrocarbons and Biochemical Changes in Oyster, Crassostrea gigas: a model study with fluoranthene (참굴에서의 Polycyclic Aromatic Hydrocarbons의 축정성과 생화학적 변화I: Fluoranthene을 model 물질로 한 연구)

  • 서영호;정의영;김강전;임완수;김희연;류동기;최선남;황인영;김정상
    • The Korean Journal of Malacology
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    • v.14 no.2
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    • pp.103-111
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    • 1998
  • 참굴에 다환성방향족탄화수소(PAHs)의 축적성과 이 물질들이 미치는 독성을 평가하기 위해 해양환경에서 빈번히 검출되는 PAHs 중의 하나인 fluoranthene을 사용한 모델연구를 수행하였다. Fluoranthene을 0.01-1ppm의 농도로 2주간 참굴에 노출시켰을 때 참굴 조직내의 fluoranthene의 농도는 노출기간 및 노출농도에 비례하여 최고 40-70배까지 증가하였다. 소화맹낭과 폐각근의 지질 과산화물가는 노출기간 및 노출농도에 관련하여 현저히 증가하였으며 수용성단백질의 함량은 노출기간이 길어짐에 따라 서서히 감소하였다. 그러나 glycogen 함량, nucleoside/nucleotide, DNA 및 RNA 함량은 변화하지 않았다. 이 결과로 fluoranthene은 굴의 조직내로 원활하게 축적함을 확인하였다. 또한 과산화지질의 함량은 fluoranthene에의 오염에 대해 신속히 반응하는 지표의 하나로 추정되며 수용성단백질의 감소도 비록 시간적으로 다소 둔감하기는 하나 또 다른 지표가 될 수 있을 것으로 사료된다.

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A Genetic Analysis of the Common Squid, Todarodes pacificus in the Korean Waters

  • Kim, Yeong-Hye;Kang, Yong-Joo;Park, Jung-Youn
    • The Korean Journal of Malacology
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    • v.18 no.1
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    • pp.9-14
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    • 2002
  • In order to estimate the genetic variability and differentiation in common squid, eleven isozymic loci, coded for nine enzymes detected by starch gel electrophoresis, were scored from nine spawning cohorts in four localities. The expected average heterozygosity ranged from 0.00019 (between II-S$_2$ and Na-W) to 0.00814 (Between Bu-S and Na-W) in nine different spawning cohorts in four localities. A dendrogram, based on genetic distance mentioned, illustrated that nine different spawning cohorts were divided into three groups, similar to the result estimated by their ecological characterizations. From these results, we estimate that the common squid distributed throughout Korean waters will maintain this gene exchange. It is postulated that either the summer or the autumn spawning cohort has developed a local population that is isolated by hydrographic factors.

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Chromosomal Studies of Two Species of Diplommatina (Mesogastropoda: Diplommatinidae) in Korea (깨알달팽이 속 (Diplommatina) 2종의 염색체연구)

  • 이준상;권오길
    • The Korean Journal of Malacology
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    • v.18 no.1
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    • pp.23-26
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    • 2002
  • Comparative karyological analyses of the Korean land snail, Diplommatina (Sinica) paxillus and Diplommatina (Sinica) changensis, were peformed by the Giemsa-staining and air-drying method. The karyotypes of both species were the same (2n = 26). However, the chromosome lengths and arm ratios, and relative chromosome lengths of the two species were distinctly different.

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Chromosome Study of Two Similar Lymnaeid Snail Species, Korean Austropeplea ollula and an Exotic Species in Australia (Pulmonata: Lymnaeidae)

  • Younghun Jung;Park, Gab-Man;Chung, Pyung-Rim
    • The Korean Journal of Malacology
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    • v.18 no.1
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    • pp.61-65
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    • 2002
  • The chromosomes of two similar lymnaeid snail species, Austropeplea ollula from Korea and "Lymnaea"sp. introduced to Australia, were karyologically investigated by using an air-dry method. The diploid chromosome number found in A. ollula was 32, and that of "Lymnaea" sp. was 30. The mitotic chromosome complements of A. ollula were 5 metacentric, 9 submetacentric, and 2 subtelocentric pairs. "Lymnaea" sp. had 5 metacentric, 8 submetacentric, and 2 subtelocentric pairs. Austropeplea ollula is distinguishable from "Lymnaea" sp. by their chromosome numbers.

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Comparative Anatomy of the Family Bithyniidae (Prosobranchia : Mesogastropoda)

  • Kim, Jae-Jin
    • The Korean Journal of Malacology
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    • v.21 no.2 s.34
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    • pp.133-145
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    • 2005
  • Eight species of bithyniids, Bithynia leachi, B. tentaculata, B. siamensis, B. misella, B. kiusiuensis, B. striatula, Bithynia sp. from Nepal and Gabbia australis were collected from 1957 to 1988. The samples were relaxed with pentobarbital and fixed with 70% ethyl alcohol. There was no significant difference in internal structure of soft body, except the male reproductive system. The alimentary canal of the bithyniids consisted of mouth, buccal mass, esophagus, stomach, intestine, rectum and anus. The heart consisted of an auricle and a ventricle, located at left side of the stomach in the visceral hump. The ctenidium comprised 40-70 subtriangular lamellae. Eight ganglia were located around upper esophagus. The female reproductive system comprised ovary, oviduct, spermaaheca, and albumin gland. The male reproductive system was composed of testis, seminal vesicle, prostate gland, vas deferens, penial duct, and penis in order. Male was distinguished externally from the female by a bifid penis.

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Bioconcentration of Polycyclic Aromatic Hydrocarbons and Biochemical Changes in Oyster, Crassostrea gigas: a model study with anthracene (참굴에서의 Polycyclic Aromatic Hydrocarbons의 축적성과 생화학적 변화II: Anthracene을 model 물질로 한 연구)

  • 서영호;정의영;김강전;배주현;류동기;황인영;김정상;박관하
    • The Korean Journal of Malacology
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    • v.14 no.2
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    • pp.113-120
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    • 1998
  • 참굴에 다환성방향족탄화수소(PAHs)의 축적성과 이 물질들이 미치는 독성을 평가하기 위해 해양환경에서 빈번히 검출되는 PAHs 중의 하나인 anthracene을 사용한 모델연구를 수행하였다. Anthracene을 0.01-1ppm의 농도로 2주간 참굴에 노출시켰을 때 참굴 조직내의 anthracene의 농도는 노출기간 및 노출강도에 비례하여 최고 25-85배까지 증가하였다. 소화맹낭과 폐각근의 지질 과산화물가는 노출기간 및 노출농도에 관련하여 현저히 증가하였으나 수용성단백질, glycogen, nucleoside/nucleotide 및 핵산의 함량은 변화하지 않았다. 이 결과로 anthracene은 굴의 조직내로 원할하게 축적됨을 확인하였으며 과산화지질의 함량은 anthracene에의 오염에 대한 예민한 생화학적 지표가 될 수 있을 것으로 사료된다.

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Biomphalaria glabrata (Pulmonata: Planorbidae): A Potential Second Molluscan Intermediate Host of A Human Intestinal Fluke, Echinostoma cinetorchis (Trematoda: Echinostomatidae)

  • Chung, Pyung-Rim;Younghun Jung;Joo, Chong-Yoon
    • The Korean Journal of Malacology
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    • v.17 no.1
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    • pp.57-61
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    • 2001
  • The present study examines the potential involvement of Biomphalaria glabrata, a known molluscan intermediate host of Schistosoma mansoni, in the life cycle of Echinostoma cinetorchis, one of the echinostomes that are ubiquitous parasites of vertebrates and are of importance in human and veterinary medicine and wildlife diseases. Echinostomes can be maintained easily and inexpensively in the laboratory and provide good models for biological research ranging from the molecular to the organismal. In the present study, no echinostome cercariae were released from the B. glabrata experimentally infected with E. cinetorchis miracidia, whereas all the Biomphalaria snails infected with E. cinetorchis cercariae were found to be infected with the metacercariae. This is the first report that B. glabrata can experimentally serve as the second intermediate host of E. cinetorchis, and that it might be employed as one of the target molluscs for establishing a biological research model with E. cinetorchis in the laboratory.

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An Immunohistochemical and Ultrastructural Study of the Heavy Metals accumulated in the Kidney of an Antarctic Clam, Laternula elliptica (중금속이 축적된 남극 큰띠조개의 신장에 대한 면역조직학적 및 미세구조적 연구)

  • 이용석;안인영;김완종;정계헌
    • The Korean Journal of Malacology
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    • v.18 no.1
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    • pp.15-21
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    • 2002
  • The kidney of bivalve mollusks often contains remarkably high concentrations of both essential and non-essential metals and perform regulating and detoxicating activities. The kidney has also been proposed as a biological indicator for radioactive as well as for stable metals in the sea. The present study of the Antarctic clam, Laternula elliptica, concerns the functional morphology of the kidney epithelium, which contains highly accumulated heavy metals. The immunohistochemical and ultrastructural study was undertaken in order to find out the localization of metallothionein and heavy metals accumulated in the kidney of Laternula elliptica. In the immunohistochemical investigation, an intense metallothionein immunostaining reaction was found in the epithelial cells of the kidney of Laternula elliptica. Transmission electron microscopy showed that the epithelial cells contained numerous electron-dense inclusion bodies which were considered to be accumulated heavy metals.

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Report on Bivalve Mollusks from Beach Death Assemblages in Gangwon and Gyeongsangbuk Provinces, Korea (East Sea)

  • Konstantin A. Lutaen;Je, Jong-Geel;Shin, Sang-Ho
    • The Korean Journal of Malacology
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    • v.18 no.1
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    • pp.27-40
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    • 2002
  • Beach death assemblages of bivalve mollusks were studied with respect to their species composition in six localities along the eastern coast of Korea (Gangneung, Jumunjin, Daejin, Gallam, Jukbyeon, Hupo and Chooksan). In all, thirty-nine species belonging to 30 genera and 19 families were recorded, and species diversity between the sampling sites varied from 5 to 21. Biogeographical analysis showed that studied fauna has warm-water character with the predominance of subtropical and subtropical-lowboreal species and the presence of tropical-subtropical species (in total, 77 %), and only 23 % of mollusks found are cold-water and temperate species. The most abundant species were Septifer virgatus (Wiegmann, 1837), Mytilus galloprovinciallis Lamarck, 1819, Gomphina melanaegis Romer, 1861, Ruditapes philippinarum (Adams et Reeve, 1850), Mactra chinensis Philippi, 1847 and Spisula sachalinensis (Schrenck, 1862).

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