• Animal Bioscience
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• v.36 no.8
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• pp.1180-1189
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• 2023

Objective: Discovering the mechanism of cell specification is important to manipulate cellular lineages. To obtain lineage-specific cell lines, the target lineage needs to be promoted, and counterpart lineages should be suppressed. Embryos in the early blastocyst stage possess two different cell populations, the inner cell mass (ICM) and trophectoderm. Then, cells in the ICM segregate into epiblasts (Epi) and primitive endoderm (PrE). PrE cells in embryos show specific expression of platelet-derived growth factor (PDGF) and its receptor, PDGF receptor A (PDGFRA). In this study, we suppressed PDGF signaling using two methods (CRISPR/Cas9 injection and inhibitor treatment) to provide insight into the segregation of embryonic lineages. Methods: CRISPR/Cas9 RNAs were injected into parthenogenetically activated and in vitro fertilized embryos. The PDGF receptor inhibitor AG1296 was treated at 0, 5, 10, and 20 µM concentration. The developmental competence of the embryos and the number of cells expressing marker proteins (SOX2 for ICM and SOX17 for PrE) were measured after the treatments. The expression levels of the marker genes with the inhibitor were examined during embryo development. Results: Microinjection targeting the PDGF receptor (PDGFR) A reduced the number of SOX17-positive cell populations in a subset of day 7 blastocysts (n = 9/12). However, microinjection accompanied diminution of Epi cells in the blastocyst. The PDGF receptor inhibitor AG1296 (5 µM) suppressed SOX17-positive cells without reducing SOX2-positive cells in both parthenogenetic activated and in vitro fertilized embryos. Within the transcriptional target of PDGF signaling, the inhibitor significantly upregulated the Txnip gene in embryos. Conclusion: We identified that PDGF signaling is important to sustain the PrE population in porcine blastocysts. Additionally, treatment with inhibitors was a better method to suppress PrE cells than CRISPR/Cas9 microinjection of anti-PDGF receptor α gene, because microinjection suppressed number of Epi cells. The PDGF receptor might control the number of PrE cells by repressing the proapoptotic gene Txnip. Our results can help to isolate Epi-specific cell lines from blastocysts.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.2
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• pp.153-159
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• 2007

In our previous study, overexpression of extracellular proteinase inhibitor (Expi) gene accelerated apoptosis of mammary epithelial cells, and induced expression of B cell activating factor (BAFF) gene. In this study, we found induction of BAFF-receptor (BAFF-R) gene expression in the Expi-transfected cells. A proliferation-inducing ligand (APRIL) gene is another TNF family member and the closest known relative of BAFF. We found induction of APRIL gene expression in the Expi-overexpressed apoptotic cells. NF-${\kappa}$B gene was also induced in the Expi-overexpressed cells. Expression patterns of BAFF and APRIL pathway-related genes were examined in in vivo mouse mammary gland at various reproductive stages. Expression levels of BAFF gene were very low at early pregnancy, increased from mid-pregnancy, and peaked at lactation, and thereafter decreased at involution stages of mammary gland. Expression of BAFF-R gene was highly induced in involution stages compared to lactation stages. Thus, expression patterns of BAFF-R gene were correlated to apoptotic status of mammary gland: active apoptosis of mammary epithelial cells occurs at involution stage of mammary gland. Expression levels of NF-${\kappa}$B gene were higher in involution stages compared to lactation stages. We analyzed mRNA levels of bcl-2 family genes from different stages of mammary development. Bcl-2 gene expression was relatively constant during lactation and involution stages. There was a slight increase in bcl-xL gene expression in involution stages compared to lactation state. Bax gene expression was highly induced in involution stage. Our results suggest that signaling pathways activated by both BAFF and ARRIL in mammary gland point towards NF-${\kappa}$B activation which causes upregulation of bax.

• The Journal of Korean Physical Therapy
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• v.14 no.3
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• pp.226-237
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• 2002

This study was performed to investigate the effect of low power laser irradiation on epidermal growth factor(EGF) expression in the burned skin of the rats. Burns of about 3cm in diameter were created with 75'c water on the back of the rats, and the lesion of experimental group were irradiated on days 1, 2, and 3 postwounding. Control tensions were not irradiated. After burns, low power laser irradiation was applied by using 1000Hz, 830nm GaAlAs (Gallium-aluminum- arsenide) semiconductor diode laser. The expression of epidermal growth factor evaluated immunohistochemistry on mouse anti-EGF. The results of this study were as follows 1. In expression of EGF, the lesion of experimental group made EGF to more induce significantly than control tensions. 2. EGF immunoreactivity in burned skin were increased markedly 3 days after burns, and increased gradually from 1 day to 2 days in burns which is laser irradiation These data suggest that low power laser have wound healing effect in the burned skin of the rats.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.22 no.1
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• pp.65-80
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• 2023

In this study, a classification analysis was performed based on the type of traffic situation. The purpose was to derive the major variable factors that could represent the traffic situation. The TTI(Travel Time Index) was used as a criterion for determining traffic conditions, and analysis was performed using data generally detected by the Vehicle Detecting System(VDS). First, the major factors influencing the traffic situation were selected through factor analysis, and traffic conditions were clustered through a cluster analysis of the major factors. After that, variance analysis for each cluster was performed based on the TTI, and similar clusters were merged to categorize the type of traffic situation. The analysis derived, the maximum queue length and occupancy as major factors that could represent the traffic situation. Through this study, it is expected that efficient management of traffic congestion would be possible by just concentrating on the main variable factors that affect the traffic situation.

• Journal of Korean Medical classics
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• v.24 no.2
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• pp.161-181
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• 2011

In spite of the development of scientific and medical technology, the rate of prevalence and death of the chronic disease gets higher. Therefore people come to know prevention is more important than treatment in disease. In Western, it is nearly after 1970's when it is revealed that the major factor of chronic disease comes from habitual behaviors in everyday life. But that is common idea in Oriental Medicine since thousand years. In Oriental Medicine, the main cause of disease is composed of natural factor including climate condition, living-life factor, psycho-social factor, constitutional factor, inbody-pathologic-substance factor, and other factors including habits of using drug and medical service, injury, environmental pollutions, and occupational etiologic factors. Among these, the major factors of disease in modern society are lifestyle factors like the tendency of choosing and intaking food, the level of physical activity and labor, smoking and drinking alcohol, psychological tendency, the habits of using drug and medical service and so on. This is a practical aspect of oriental medicine where the most important etiologic factor comes from human behaviors, not from external factors like germ or virus. So that etiology in Oriental Medicine where improvement in the way of life is needed to prevent disease would play an important role in modern society.

• Journal of the Korean Data and Information Science Society
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• v.19 no.3
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• pp.761-774
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• 2008

Agility is vital to real-time enterprises in comtemporary dynamic business environment. This study aims to investigate the relationships between traditional shipping and port logistics firms' customer agility obtained by adopting Internet business, and their corporate image and customer satisfaction. Using questionnaire data, factor analyses were used to figure out five major agility factors, corporate image factor, and customer satisfaction factor. The agility factors were then used to investigate how they improve the firms' corporate image and customer satisfaction. The results of the regression analyses show that agility factors significantly influence the firms' corporate image and customer satisfaction factors.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.228.2-229
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• 2003

Estrogen Receptor is a ligand-activated transcription factor. The concentration of the receptor is a major component that regulates expression of estrogen-responsive genes. We have studied mechanism of estrogen receptor alpha (ER${\alpha}$) downregulation by HIF-1 using HIF-1${\alpha}$/VP16 constructs. ER${\alpha}$ is known to be downregulated under hypoxic condition. Transcriptional response under hypoxia is mediated through Hypoxia-inducible factor-1 (HIF-1), a transcription factor that is usullaly degraded but stabilized under hypoxia. (omitted)

• Archives of Pharmacal Research
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• v.10 no.4
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• pp.245-249
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• 1987

Cardiovascular disease is at present the leading cause of deth in the United States and other in dustrilized countries. A major contributing factor of cardiovascular disease is essential hypertension. Untreated, essential hypertension is considered a risk factor for sudden death due to myocardial infarctions, as well as a risk factor for cerebral vascular disease, renal failure and congestive heart failure. During the last decade, significant progress has been made in the basic knowledge of the pathogenesis of hypertension as well as in the development of new antihypertensive drugs.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.32 no.2
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• pp.205-214
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• 1996

• Geomechanics and Engineering
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• v.11 no.2
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• pp.235-252
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• 2016

This paper presents a new numerical solution of the ultimate lateral capacity of rectangular piles in clay. The two-dimensional plane strain finite element was employed to determine the limit load of this problem. A rectangular pile is subjected to purely lateral loading along either its major or minor axes. Complete parametric studies were performed for two dimensionless variables including: (1) the aspect ratios of rectangular piles were studied in the full range from plates to square piles loaded along either their major or minor axes; and (2) the adhesion factors between the soil-pile interface were studied in the complete range from smooth surfaces to rough surfaces. It was found that the dimensionless load factor of rectangular piles showed a highly non-linear function with the aspect ratio of piles and a slightly non-linear function with the adhesion factor at the soil-pile interface. In addition, the dimensionless load factor of rectangular piles loaded along the major axis was significantly higher than that loaded along the minor axis until it converged to the same value at square piles. The solutions of finite element analyses were verified with the finite element limit analysis for selected cases. The empirical equation of the dimensionless load factor of rectangular piles was also proposed based on the data of finite element analysis. Because of the plane strain condition of the top view section, results can be only applied to the full-flow failure mechanism around the pile for the prediction of limiting pressure at the deeper length of a very long pile with full tension interface that does not allow any separation at soil-pile interfaces.