• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.20 no.11
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• pp.1071-1077
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• 2010

The reduction of the vehicle interior noise has been the main interest of noise and vibration harshness(NVH) engineers. A passenger vehicle has various and complicated transmission paths of sound and vibration. In order to identify the mechanism of transfer path, estimation of excitation force and exact modeling of transfer path are required. This paper presents method for estimating the noise source contribution on the road noise of the vehicle in a multiple input system where the input sources may be coherent with each other. And vector synthesis technique is employed to identify the characteristics of road noise and its transmission to vehicle compartment through noise and vibration analysis. Vibration reduction efficiency of each transfer path is evaluated by comparing individual vector components obtained virtual simulation.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.9
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• pp.4567-4587
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• 2017

With the growth of the Internet and the extensive applications of image editing software, it has become easier to manipulate digital images without leaving obvious traces. Copy-move is one of the most common techniques for image forgery. Image blind forensics is an effective technique for detecting tampered images. This paper proposes an improved copy-move forgery detection method based on the discrete cosine transform (DCT). The quantized DCT coefficients, which are feature representations of image blocks, are truncated using a truncation factor to reduce the feature dimensions. A method for judging whether two image blocks are similar is proposed to improve the accuracy of similarity judgments. The main transfer vectors whose frequencies exceed a threshold are found to locate the copied and pasted regions in forged images. Several experiments are conducted to test the practicability of the proposed algorithm using images from copy-move databases and to evaluate its robustness against post-processing methods such as additive white Gaussian noise (AWGN), Gaussian blurring, and JPEG compression. The results of experiments show that the proposed scheme effectively detects both copied region and pasted region of forged images and that it is robust to the post-processing methods mentioned above.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.163-169
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• 2001

Despite the high potency of the retrovirus vector system in gene transfer, one of the main drawbacks of has been difficulty in preparing highly concentrated virus stock. Numerous efforts to boost the virus titer have ended in unsatisfactory results mainly due to fragile property of retrovirus envelope protein. In this study, to overcome this problem, we constructed our own retrovirus vector system producing vector viruses encapsulated with VSV-G (vesicular stomatitis virus G glycoprotein). Concentration process of the virus stock by ultracentrifuge did not sacrifice the virus infectivity, resulting in more than 108 to 109 CFU (colony forming unit) per ml on most of the target cell lines tested. Application of this high-titer retrovirus vector system was tested on chicken embryos. Injection of virus stock beneath the blastoderms of pre-incubated fertilized eggs resulted in chick embryos expressing E. coli LacZ gene with 100% efficiency. Therefore, our results suggest that it is possible to transfer the foreign gene into chicken embryo using our high-titer retrovirus vector.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.8
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• pp.1376-1385
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• 2000

The phase characteristic of delay line in feedfarward linearizer has been changed due to variation of operating temperature. In this paper, design method of fixed phase control circuit of group delay line using adaptive vector control is derived. To maintain transfer characteristics of nominal operating temperature, the error correlated signals, which are changed adaptively due to changing of temperature, are added to main signals. The proposed method maintains transfer characteristics under 0.06dB of insertion loss and 0.36$^{\circ}$ of phase variation in case of 1-tone(880 MHz) and under 0.07 dB of insertion loss and 0.35$^{\circ}$ of phase variation in case of 2-tones(877 MHz, 882 MHz) for 10dB input power dynamic range and +/-10$^{\circ}$ phase variation respectively.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.1
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• pp.26-34
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• 2008

This paper summarizes a design procedure of radiated noise from engine blocks of marine engines. This air-borne noise is one of the significant noise contributors including the aeroacoustic noise due to intake and exhaust and the re-radiation due to structure-borne noise. Excitation forces by engine operations are evaluated taking into account the power generation mechanism from the burning process to the subsequence motion of internal parts; piston, connecting rod, and crank shaft. The acoustic transfer vector method is incorporated to effectively simulate the radiated noise field under the various operation conditions. A contribution analysis for the various excitations to the radiated noise is conducted. It is found that the firing pressure is the main source of the radiated noise, and so the structure of the cylinder can be modified to significantly reduce the radiated noise from the engine block.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.333-341
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• 1996

One of the biggest problems involved in transgenic animal production is lack of appropriate market genes. To overcome this problem, we tested whether the genes of SEAP (secreted alkaline phosphatase) and GFP (green fluorescence protein) on our retrovirus vectors can be applicable to the transgenic animal production. The main advantage of these marker genes over other generally mainpulation can be selected without sacrificing viability. The results obtained in this study are summarized as follows: 1. Removal of zona pellucida from the mouse zygotes did not affect embryo developments to blastocysts. 2. Co-culture of zona-free embryos with virus-producing cells for 6 hours also did not affect embryo developments to blastocysts. 3. Among 58 blastocysts developed from the zona-free zygotes co-cultured with the virus-producing cells, SEAP expression was observed from the 6 blastocysts. 4. Expression of the GFP gene was detected from the virus- producing cells but no embryo expressing the gene was counted among 50 blastocysts developed from the zona-free zygotes co-cultured with the virus-producing cells.

• Proceedings of the IEEK Conference
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• 2002.06e
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• pp.95-98
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• 2002

This paper presents a study on DSP(TMS320F240) controller design for multi-axes transportation system using BLDC servo motor. This BLDC servo motor controller was realized with DSP(Digital Signal Processor) and IPM (Intelligent Power Module). The multi-axes transportation system needs torque, speed, position control of servo motor for variable action. This paper implements those servo control with vector control and space vector modulation technique. As CPU of controller DSP(TMS320F240) is adopted because, it has PWM(Pulse Width Modulation) waveform generator, A/D(Analog to Digital) converter, SPI(Serial Peripheral Interface) port and input/output port etc. The controller of multi-axes transportation system consists of 3-level hierarchy structure that main host PC manages three sub DSP system which transfer downword command and are monitoring the states of end servo controllers. Each sub DSP system operates eight BLDC servo controllers which control BLDC servo motor using DSP and IPM Between host system and middle digital signal processor communicate with RS-422, between main processor and controller communicate with SPI port.

• Proceedings of the KSRS Conference
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• 2007.10a
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• pp.153-155
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• 2007

Korea Meteorological Administration (KMA) has operationally produced Atmospheric Motion Vector (AMV) from the consecutive MTSAT-1R satellite image dataset. Comparing with radiosonde data, our current AMV scheme shows more than 10 m/s RMSE. Therefore we need to improve continuously its accuracy. Many AMV producers have stated that the bad performance of the Height Assignment (HA) algorithm is the main reason of degrading the accuracy of AMV. The uncertainties in AMV HA can occur in the algorithm itself, used NWP profiles, and the performance of Radiative Transfer Model (RTM) etc. This study introduces currently operated AMV HA schemes and the impacts of NWP profile data and RTM that these schemes use were investigated. Finally we analyzed the relationship between vectors by vector tracking and heights assigned to each vector by using collocated wind profile dataset with radiosonde data. This study is a preliminary work to improve the accuracy of AMV by removing or decreasing the uncertainties in AMV estimation.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.4
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• pp.308-316
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• 2006

Objectives Despite considerable advances in technique, experience and skill, the precise place of surgery in the treatment of facial nerve injury remains uncertain. We designed a facial nerve crush injury model in rats and evaluated the recovery of crushed nerve which is the most common injury type of facial nerve using adenovirus vector mediated in vivo gene transfer of Brain derived neurotrophic factor(BDNF). Materials and methods In 48 Sprague Dawley rats, we made a facial nerve crush injury model to main trunk before the furcation, and injected a $10^{11}$pfu adenoviral BDNF in experimental group(BDNF adenoviral injection group; ad-BDNF) and $3{\mu}l$ saline in control group(Saline injection group; saline). After a period of regeneration from 10 to 40 days, nerve regeneration was evaluated with functioinal test (vibrissae and ocular movement), electrophysiologic study(threshold, peak voltage, conduction velocity) and histomorphometric study of axon density. Results Vibrissae and ocular movement, threshold and conduction velocity improved as time elapse in both group, however axon density was increased significantly only in experimental group. Functional test in 10 days and 20 days showed no difference between experimental group and control group. Vibrissae movement, threshold, conduction velocity and axon density in 30 days revealed that the regeneration in quality of experimental group was significantly superior to that of control group. Conclusion In general, there is tendency for nerve regeneration in experimental group (BDNF-adenovirus injection group) during 40 days, functional recovery was detected successfully after facial nerve crush in 30 days postoperatively.

• Korean Journal of Head & Neck Oncology
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• v.19 no.1
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• pp.25-33
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• 2003

Background and Objectives: The Herpes Simplex type 2 Defective Infectious Single Cycle virus (DISC virus) is attenuated virus originally produced as viral vaccines but are also efficient gene transfer vehicle. The main goals of this study were to examine the efficiencies of the gene transfer using DISC vectors for various head and neck squamous cell carcinoma cell lines and to evaluate the efficacy of vaccination with DISC virus carrying a immunomodulatory genes (GM-CSF) as cancer therapy in a organotopic oral cavity squamous cell cancer model. Materials and Methods : We determinated the gene transfer efficiency of DISC virus by x-gal stain method and proved gene and protein expression of DISC-GMCSF transfected SCCVII cells by RT-PCR and ELISA method. Also we evaluated the ex vivo vaccination effects of SCCVII/GMCSF (DISC-GMCSF transfected SCCVII vaccine) vaccine on preventing the recurrence of micro-residual tumor. After the vaccination of SCCVII/GMCSF, specific cytotoxic T-cell responses was evaluated by CTL assay. Results: At an MOI of 10 DISC virus showed 64-88% of transfection rates in various head and neck squamous cancer cell lines. SCCVII cells transduced by DISC virus vector (MOI=10) carrying the GM-CSF gene, produced 4.5 nanogram quantities of GM-CSF per $10^6$ cells. In vivo vaccination using tumor cells transduced ex vivo with DISC-GMCSF resulted in better protection rate against subsequent tumor recurrence in organotopic oral cavity cancer model. Although tumor free survival rate was not statistically significantly increased in vaccination group (p=0.078), tumor specific cytotocic T-cell responses were significantly increased in SCCVII/GMCSF vaccination group. Conclusion: These data demonstrate that; 1) The DISC virus vector is capable of efficient gene transfer to various head and neck squamous cancer cell lines, 2) GM-CSF secreting genetically modified tumor vaccine (SCCVII/GMCSF) efficiently protected against tumor recurrence in organotopic micro-residual oral cavity cancer model and produced tumor specific cytotoxic T-cell response. DISC virus-mediated, cytokine gene transfer may prove to be useful as a clinical therapy for head and neck cancers.