• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.206-206
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• 2017

Sorghum is a crop with a various plant height depending on the planting density. If the height exceeds 1.8m, which is the harvestable height of the combine, loss is caused by clogging of the installation, entrance of the threshing section and the threshing section. The purpose of this study is to set the planting distance and number of plants per hill suitable for combine harvesting as the plant length does not exceed 1.8m. The experimental variety was Nampungchal. The experiment design was a split-plot design with three replications. The treatments were as follow: Main-plot were 1 and 2 plants as number of plants per hill and sub-plots were $60{\times}20cm$ (practice), $70{\times}15$, 20, 25, 30 cm as planting distance. The amount of nitrogen, phosphate and potassium fertilization were 100, 70, $80kg\;ha^{-1}$. Data were collected: (1) grain yield: weight of grain in $kg\;ha^{-1}$, (2) 1000 grain weight: average weight of 1000 grain, (3) plant height: distance from soil to top of panicle, (4) ear length: distance from top of stem to top of ear in cm, (5) stem diameter: diameter of second internode, (6) tiller number per hill. Analyses of variance were performed using R version 3.3.1(https://www. r- project. org). The Duncan's multiple range test(DMR) was used to separate treatment means at P < 0.05. As number of plants per hill increased, plant height and yield increased and tiller number decreased. As planting distance increased, plant height and yield decreased and tiller number increased. At 1 plant per hill, the plant height did not exceed 1.8m at all planting distance. At 2 plants per hill, the plant height did not exceed 1.8m from the planting distance of $70{\times}25cm$. At 1 plant per hill, the tiller number increased to 0.23, 0.27, 0.60 and 0.70 as the planting distance increased to $70{\times}15$, 20, 25 and 30 cm, respectively. At 2 plants per hill, the tiller number increased to 0.03, 0.03, 0.14 and 0.40 as the planting distance increased to $70{\times}15$, 20, 25 and 30 cm, respectively. At 1 plant per hill, the yield decreased to 6030, 4280, 3400 and $3230kg\;ha^{-1}$ as the planting distance increased. At 2 plant per hill, the yield decreased to 7850, 5770, 5720 and $4960kg\;ha^{-1}$ as the planting distance increased. We recommend that the optimum number of plants per hill and planting distance is 2 and $70{\times}25cm$ suitable for combine harvesting.

• ALGAE
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• 제18권2호
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• pp.129-134
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• 2003

In order to examine the effects of deep seawater (mesopelagic water in the broad sense) on the growth of macroalgae, the growth and nutrient uptake (nitrate and phosphate) of Ulva sp. (Ulvophyceae, Chlorophyta) were investigated by cultivation in deep seawater (taken from 687 m depth at Yaizu, central Japan, in August 2001), surface seawater (taken from 24 m depth), and a combination of the two. Culture experiments were carried out in a continuous water supply system and an intermittent water supply system, in which aerated 500-mL flasks with 4 discs of Ulva sp. (cut sections of ca. 2 $cm_2$) were cultured at 20$^{\circ}C$ water temperature, 100 $\mu$mol photons $m^{-2}{\cdot}s^{-1}$ light intensity, and a 14:10 light:dark cycle. Nutrient uptake by Ulva sp. was high in all seawater media in both culture systems. The frond area, dry weight, chlorophyll a content, dry weight per unit area, and chlorophyll a content per unit area of Ulva sp. at the end of the experimental period were the highest in deep seawater and the lowest in surface seawater in both culture systems. These values, except for dry weight per unit area and chlorophyll a content per unit area, for each seawater media in the intermittent water supply system were higher than those in the continuous water supply system. We conclude that not only deep seawater as the culture medium but also the seawater supply system is important for effective cultivation of macroalgae.

• 한국유기농업학회지
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• 제13권2호
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• pp.197-209
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• 2005

of Hangalku per plant sold for wild vegetables were 9.1g and 0.9g, and number of leaves was 10.8. Root fresh and dry weights of Hangalku per plant were 19.2g and 4.1g. Thirty five soil samples were collected from the native soils grown Cirsium japonicum DC., and mycorrhizal spores in soils were separated using wet-sieving methods. Number of mycorrhizal spores per 30g fresh soil sized over 500${\mu}$m, 355~500${\mu}$m, 251~354${\mu}$m, 107~250${\mu}$m and 45~106${\mu}$m were 0.6, 2.1, 6.0, 55.3 and 126, etc. Total number of mycorrhizal spores per 30g fresh soil were 190. Root infection by vesicles, hyphae and arbuscules were 13%, 4% and 3%, respectively. As a result of identification, mass propagated mycorrhizal spores by the host plant of Sudangrass were Glomus sp., Gigaspora sp., and Acaulospora sp., and so on.

• BMB Reports
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• 제28권5호
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• pp.375-381
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• 1995

Two forms of glucoamylase (GI and GII) from starch-grown Lipomyces kononenkoae CBS 5608 mutant were purified to apparent homogeneity by means of ultrafiltration, Sephacryl S-200 gel filtration and DEAE Sephadex A-50 chromatography. The apparent molecular weight was calculated as ca. 150 kDa for GI and ca. 128 kDa for GII, respectively. Both enzymes were glycoproteins with isoelectric points of 5.6 (GI) and 5.4 (GII). They had a pH optimun of 4.5 and were stable from pH 5 to 8. The temperature optimum for both enzymes was $60^{\circ}C$, but they were rapidly inactivated above $70^{\circ}C$. The $K_m$ values toward starch were estimated to be 6.57 mg per ml for GI and 4.52 mg per ml for GII, and the $V_{max}$ values were 16.28 ${\mu}M$ per mg for GI and 32.25 ${\mu}M$ per mg for GII, respectively. The $K_m$ and $V_{max}$ values of GII for ${\alpha}-$ or ${\beta}-cyclodextrin$ were estimated to be 0.15 mg per ml and 2.0 mg per ml, respectively ($K_m$) and 1.02 ${\mu}M$ per mg or 1.02 ${\mu}M$ per mg, respectively ($V_{max}$). Neither enzyme exhibited pullulanase activity but they released only glucose from starch or cyclodextrin. Amino acid analysis indicated that both glucoamylases were enriched in proline and acid amino acids. Glucoamylase GII strongly cross-reacted with a monoclonal antibody raised against GI enzymes, and the two enzymes shared very similar amino acid composition. Western blot analysis indicated that L. kononenkoae CBS 5608 mutant produced two forms of glucoamylase on starch, and that synthesis of them was subject to glucose repression.

• The Korean Journal of Pain
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• 제35권4호
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• pp.413-422
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• 2022

Background: The neocortex, including the medial prefrontal cortex (mPFC), contains many neurons expressing nitric oxide synthase (NOS). In addition, increasing evidence shows that the nitric oxide (NO) and opioid systems interact in the brain. However, there have been no studies on the interaction of the opioid and NO systems in the mPFC. The objective of this study was to investigate the effects of administrating L-arginine (L-Arg, a precursor of NO) and N(gamma)-nitro-L-arginine methyl ester (L-NAME, an inhibitor of NOS) into the mPFC for neuropathic pain in rats. Also, we used selective opioid receptor antagonists to clarify the possible participation of the opioid mechanism. Methods: Complete transection of the peroneal and tibial branches of the sciatic nerve was applied to induce neuropathic pain, and seven days later, the mPFC was cannulated bilaterally. The paw withdrawal threshold fifty percent (50% PWT) was recorded on the 14th day. Results: Microinjection of L-Arg (2.87, 11.5 and 45.92 nmol per 0.25 µL) increased 50% PWT. L-NAME (17.15 nmol per 0.25 µL) and naloxonazine (an antagonist of mu opioid receptors, 1.54 nmol per 0.25 µL) inhibited anti-allodynia induced by L-Arg (45.92 nmol per 0.25 µL). Naltrindole (a delta opioid receptor antagonist, 2.45 nmol per 0.25 µL) and nor-binaltorphimine (a kappa opioid receptor antagonist, 1.36 nmol per 0.25 µL) were unable to prevent L-Arg (45.92 nmol per 0.25 µL)-induced antiallodynia. Conclusions: Our results indicate that the NO system in the mPFC regulates neuropathic pain. Mu opioid receptors of this area might participate in pain relief caused by L-Arg.

• 한국산학기술학회논문지
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• 제16권4호
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• pp.2924-2932
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• 2015

본 연구는 초등학교, 중학교 및 고등학교의 급수설비 사용수량의 분석 통해 급수량 산출자료를 추정하여 학교시설 급수시스템의 적정 급수계획에 필요한 기초자료를 얻고자 하였다. 이를 위하여, 강원지역 6개 도시에 소재하는 초 중 고등학교 54개 학교를 대상으로 5년간의 급수설비 사용수량을 분석하였다, 분석결과, 1) 월평균 사용수량의 평균은 $600.7(m^3/mon)$이었으며, 초등학교와 중학교가 $484.2(m^3/mon)$, 고등학교가 $833.8(m^3/mon)$로 분석 되었다. 2) 학급당 월평균 사용수량의 평균은 $27.0(m^3/mon/class)$이었으며, 초등학교와 중학교가 $24.4(m^3/mon/class)$, 고등학교가 $32.(m^3/mon/class)$로 분석 되었다. 3) 1인1일 평균 사용수량의 평균은 55.8(l/c/d)이었으며, 초등학교와 중학교가 54.4(l/c/d), 고등학교가 58.6(l/c/d)로 분석 되었다.

• 한국미생물·생명공학회지
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• 제3권1호
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• pp.41-48
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• 1975

시판정제효소제(당정)와 L. bulgaricus를 이용하여 탁주류를 제조하면서 성분분석과 품질을 검토하였다. 1. 유기산의 함양에 있어서, 밀가루, 옥수수, 팔, 눌린 보리쌀 등 4종류의 원요를 통하여 917~1,350mg/ml의 lactic acid가 생성되었으며 succinic acid는 6~32mg/100m1이다. 종내의 누주중의 유기담으로서 는 lactic acid와 succinic acid가 대표적으로 많이 합유된 유기산인데 비하면 suceinic acid의 함양은 현저하게 적다. 이외에 종내의 탁주에서 검출되여 알려진 유기산에는 citric acid, malic acid, maleic acid, fumaric, malonic acid, oxalic acid 등이 있으며, citric acid와 malic acid는 비교적 그외의 산보다 많은데 비하여 본실험의 것에서는 fumaric acid와 malonic acid는 검출되지 않았였고 눌린 보리쌀을 사용했을때, 6.5mg/100m1의 citric acid를, 그리고 밀가루와 옥수수를 사용하였을때 각각 1.7 mg/100ml와 1.4 mg/ml의 malic acid를 생성하였을뿐 그외는 흔적으로 검출되었다. 2. fusel oil의 조성은 원요와 효소제의 종유에 관계없이 대동소이하다. 즉, i-amyl alcohol는 (1.50~2.77)$\times$$10^{-2}$g/100m1로서 공통적으로 가장 많고, i-butyl alcohol는 (0.68~1.30)$\times$$10^{-2}$g/100m1이며 n-propyl alcohol는 (0.20~0.34)$\times$$10^{-2}$g/100m1, $\beta$-phenyl ethyl alcohol는 0.18$\times$$10^{-2}$g/100m1 이하의 순으로 적다. 종내의 효소제에 의한 것도 누룩을 사용하였을때 0.70$\times$$10^{-2}$g/100m1의 $\beta$-phenyl ethyl alcohol을 예외로 하면 각각 상기범전에 들어있다. 3. 아미노산의 조성분은 홍등의 실험에서와 같이 17종을 함유하고 있으며 총아미노기양에 있어서 본실험의 것이 31mg/100m1로서 비교적 적으나 주유의 조미성분으로서 중요한 glycine과 alanine이 0.6023 mg/100m1와 4.3129 ml/100ml 검출되었다.

• 농업과학연구
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• 제23권1호
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• pp.159-176
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• 1996

This study is aimed at reviewing the methods of joint cost allocation and allocating the joint cost of estuary dam with specially repect to Kumgang Large-scale Agricultural Comprehensive Development Project. Apart from the water resource development project propelled by Water Resource Development Corporation in connection with Law of Multipurpose Dam Development, the Largescale Comprehensive Agricultural Development Projects couldn't ins-titutionally be carried out cost allocation of common facilities, even though it were concerned with irrigation, municipal and industrical water supply, flood control, sightseeing and industrial zone development components. To decrease farmer's burden of the project costs and, operation and maintenance costs, the joint costs of common facilities like estuary dam included in agricultural development projects have to be allocated by suitable method as alternative cost-remaining benefit method and the analytical activity should be supported by revising the concerned laws as Rural Development and Promotion and, Rural Rearrangement conpatible with the law for multipurpose dam development. Kumgang Agricultural Comprehensive Development Project was selected as a case study for the estimation of socio-economic benefits by project components and joint cost allocation of the estuary dam. The main results of the study are as follows; Joint cost allocation and unit charges by components 1. The project area will be 25,554ha with total project cost of 624,860 million won including the estuary dam cost of 120,843 million won. The project costs were ex-pressed by 1994 constant price. 2. Total quantity of water was estimated 365 million tons which were consisted of 245 million tons for irrigation, 73 million tons for municipal water and 47 million tons for industrial water. 3. The rates of joint cost allocation were amounted to 34.2% for agriculture, 2.5% for sightseeing, 45.7% for transportation, 11.8% for M & I water supply and 5.8% for flood control respectively. 4. The unit financial charges by project components were estimated at 7.88 won per ton for irrigation, 16.11won for M & I water, 1,686won per vehicle one pass, 977won per Pyeong according to the capital recovery method. The financial charges using straitline method for depreciation were estimated at 7.88won per ton for irrigation, 9.12won per ton for M & I water, 624won per vehicle one pass for transportation and 331won per Pyeong for sightseeing area. 5. The unit economic charges by project components were estimated at 21.1 won per ton for irrigation, 15.2won for M & I water, 977won per vehicle one pass, 977won per Pyeong according to the capital recovery method. The economic charges using straitline method for depreciation were estimated at 11.72won per ton for irrigation, 8.61won per ton for M & I water, 331won per vehicle one pass for transportation. Policy recommendation 1. The unit operation and maintenance costs for irrigation water in the paddy field couldn't be imposed as the water resource cost untreated. 2. The dam costs including investment cost and O & M cost, as a joint cost, had to be allocated by each benefited components as transportation, M & I water supply, flood control, irrigation and drainage, and sightseeing. But the agricultural comprehensive project have been dealt as an irrigation project without any appraisal socio-economic benefits and any allocating the joint cost of estuary dam. 3. All the associated project benefits and costs must be evaluated based on accounting principle and rent recovery rate of the project costs and O & M costs should be regulated by the laws concerned. 4. The rural development and promotion law and rural rearrangement law have to be revised comprising joint cost allocation considering free rider problems. 5. The government subsidy for the agricultural base development project has to be covered all the project costs. In case of common facilities representing joint cost allocation problems, all the allocated casts for other purposes like transportation and M & I water supply etc. should be recovered for formation in investment fund for agricultural base development and to procure O & M costs for irrigation facilities.

• 한국응용곤충학회지
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• 제51권2호
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• pp.83-90
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• 2012

반촉성재배 시설고추에 발생하는 4종의 해충에 대한 천적이용 효과를 660 $m^2$의 면적에서 조사하였다. 진딧물은 3포트의 진디벌-뱅커플랜트와 콜레마니진디벌(Aphidius colemani)을 $m^2$당 4월16일 0.8 마리와 4월 23일 2.3 마리를 방사하여 진딧물을 성공적으로 방제할 수 있었다. 총채벌레 방제를 위해 미끌애꽃노린재(Orius laevigatus)를 5월 11일과 6월 12일 $m^2$당 총 3.0 마리를 방사하여 작기 동안 총채벌레 밀도를 꽃 당 0.3 마리 이내로 억제하였다. 지중해이리응애(Amblyseius swirskii)를 5월 11일과 5월 30일 $m^2$당 총 113.7 마리를 방사하여 작기동안 담배가루 이 밀도를 트랩 당 171.0마리 이내, 차응애 밀도는 잎 당 0.8 마리 이내로 억제하였다. 차먼지응애는 지중해이리응애 방사 12일 후에 완전방제 되었다. 4종 해충의 방제에 소요된 천적사용 비용은 660 $m^2$당 42만원이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제7권3호
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• pp.343-346
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• 1994

A research study was undertaken to quantify fascioliasis in both live and slaughtered zebu cattle at Savar, Bangladesh. Eggs of Fasciola gigantica per gram of feces (EPG) was determined in a total of 213 fasciola infested live cattle. The EPG per animal ranged from 100 to 400 (mean $138.03{\pm}4.27SE$). Counting of F. gigantica was made in a total of 63 fasciola infested livers of slaughtered cattle. Number of immature flukes per liver ranged from 0 to 37 (mean $8.74{\pm}0.85SE$) and mature flukes ranged from 2 to 121 (mean $20.54{\pm}2.23SE$). Total load of flukes recovered per liver varied from 4 to 132 (mean $29.28{\pm}2.42SE$). Significantly higher EPG (p < 0.05) and higher load of flukes in the livers (p < 0.01) were observed from September to December (post monsoon and winter). The EPG and fluke counts were found significantly higher (p < 0.01) in animals after one year of age and these were also higher in female animals (p < 0.05) than the males.